PKHO1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PKHO1_MOUSE
• UniProt AC: Q9JIY0
• Protein Name: Pleckstrin homology domain-containing family O member 1
• Gene Name: Plekho1
• Organism: Mus musculus (Mouse).
• Sequence Length: 408
• Subcellular Localization: Cell membrane ; Peripheral membrane protein . Nucleus . Cytoplasm . Predominantly localized to the plasma membrane through the binding to phosphatidylinositol 3-phosphate. In C2C12 cells, with the absence of growth factor, it is found in the nucleus.
• Protein Description: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation (By similarity)..
• Protein Sequence: MKKSGSGKRGPPDGNHQSAAPEKVGWVRKFCGKGIFREIWKNRYVVLKGDQLYVSEKEVKDEKNSQEVFDLSDYEKCEELRKSKSRSKKNHSKFTLARCRQPGTTAPNLIFLAVSPEEKESWINALSSAITRAKNRILDEVTVEEDSYLAHPTRDRAKIQHSRRPPTRGHLMAVASTSTSDGMLTLDLIQEEDPSPEEPASCAESFRVDLDKSVAQLAGSRRRADSDRIQPSSQRASSLSRPWEKPDKGAPYTPQALKKFPSTEKSRCASLEEILSQRDTAPARPLHLQAEESLPPVPAQPGQLSRIQDLVARKLEKTQELLAEVQGLGDGKRKAKDPPQSPPDSESEQLLLETERLLGEASSNWSQAKRVLQEVRELRDLYRQMDLQTPDSHLRQTSQHSQYRKSLM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Phosphorylation	----MKKSGSGKRGP ----CCCCCCCCCCC	32.45	30635358
6	Phosphorylation	--MKKSGSGKRGPPD --CCCCCCCCCCCCC	48.51	30635358
153	Phosphorylation	DSYLAHPTRDRAKIQ CCCCCCCCCCCHHHC	35.02	28059163
213	Phosphorylation	FRVDLDKSVAQLAGS HCCCCCHHHHHHHHH	23.50	28066266
220	Phosphorylation	SVAQLAGSRRRADSD HHHHHHHHHCCCCCC	19.60	19144319
226	Phosphorylation	GSRRRADSDRIQPSS HHHCCCCCCCCCCCH	28.01	26824392
233	Phosphorylation	SDRIQPSSQRASSLS CCCCCCCHHHHHHCC	30.30	-
237	Phosphorylation	QPSSQRASSLSRPWE CCCHHHHHHCCCCCC	33.72	23737553
238	Phosphorylation	PSSQRASSLSRPWEK CCHHHHHHCCCCCCC	29.40	27180971
240	Phosphorylation	SQRASSLSRPWEKPD HHHHHHCCCCCCCCC	37.94	23737553
252	Phosphorylation	KPDKGAPYTPQALKK CCCCCCCCCHHHHHH	30.44	28066266
253	Phosphorylation	PDKGAPYTPQALKKF CCCCCCCCHHHHHHC	14.09	27180971
262	Phosphorylation	QALKKFPSTEKSRCA HHHHHCCCCCHHHCC	52.94	22942356
263	Phosphorylation	ALKKFPSTEKSRCAS HHHHCCCCCHHHCCC	47.90	22942356
266	Phosphorylation	KFPSTEKSRCASLEE HCCCCCHHHCCCHHH	26.76	23984901
270	Phosphorylation	TEKSRCASLEEILSQ CCHHHCCCHHHHHHC	39.68	23527152
314	Ubiquitination	IQDLVARKLEKTQEL HHHHHHHHHHHHHHH	51.79	22790023
341	Phosphorylation	KAKDPPQSPPDSESE CCCCCCCCCCCCHHH	44.14	29899451
345	Phosphorylation	PPQSPPDSESEQLLL CCCCCCCCHHHHHHH	49.12	25293948
347	Phosphorylation	QSPPDSESEQLLLET CCCCCCHHHHHHHHH	34.30	25293948
354	Phosphorylation	SEQLLLETERLLGEA HHHHHHHHHHHHHHH	26.78	20531401
397	Phosphorylation	PDSHLRQTSQHSQYR CCHHHHHHHHHHHHH	24.74	30635358
398	Phosphorylation	DSHLRQTSQHSQYRK CHHHHHHHHHHHHHH	19.98	30635358
401	Phosphorylation	LRQTSQHSQYRKSLM HHHHHHHHHHHHHHC	22.67	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
341	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
341	S	Phosphorylation	Kinase	GSK3B	Q9WV60	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PKHO1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PKHO1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
IN35_MOUSE	Ifi35	physical	17197158
NMI_MOUSE	Nmi	physical	17197158
PKHO1_MOUSE	Plekho1	physical	17197158
GSK3B_MOUSE	Gsk3b	physical	24777252

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"The phagosomal proteome in interferon-gamma-activated macrophages.";
Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.;
Immunity 30:143-154(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220; SER-226 ANDSER-270, AND MASS SPECTROMETRY.
PubMed: 19144319