dbPTM

ENOA_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ENOA_MOUSE
UniProt AC	P17182
Protein Name	Alpha-enolase
Gene Name	Eno1
Organism	Mus musculus (Mouse).
Sequence Length	434
Subcellular Localization	Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity). ENO1 is localized to the M-band.
Protein Description	Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses (By similarity). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production (By similarity)..
Protein Sequence	MSILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSQAVEHINKTIAPALVSKKVNVVEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKTAIAKAGYTDQVVIGMDVAASEFYRSGKYDLDFKSPDDPSRYITPDQLADLYKSFVQNYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAASEKSCNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSKAKFAGRSFRNPLAK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSILRIHAR ------CCCEEEEEH	30.26	-
2	Phosphorylation	------MSILRIHAR ------CCCEEEEEH	30.26	29514104
14	Phosphorylation	HAREIFDSRGNPTVE EEHHHHHCCCCCEEE	31.19	20469934
19	Phosphorylation	FDSRGNPTVEVDLYT HHCCCCCEEEEEEEE	33.04	25367039
25	Phosphorylation	PTVEVDLYTAKGLFR CEEEEEEEECCCHHH	10.53	25177544
26	Phosphorylation	TVEVDLYTAKGLFRA EEEEEEEECCCHHHH	29.23	25266776
28	Acetylation	EVDLYTAKGLFRAAV EEEEEECCCHHHHHC	48.63	22826441
28	Succinylation	EVDLYTAKGLFRAAV EEEEEECCCHHHHHC	48.63	23954790
28	Ubiquitination	EVDLYTAKGLFRAAV EEEEEECCCHHHHHC	48.63	-
37	Phosphorylation	LFRAAVPSGASTGIY HHHHHCCCCCCCCHH	40.15	28542873
40	Phosphorylation	AAVPSGASTGIYEAL HHCCCCCCCCHHHHH	30.60	25521595
41	Phosphorylation	AVPSGASTGIYEALE HCCCCCCCCHHHHHH	27.37	25521595
44	Phosphorylation	SGASTGIYEALELRD CCCCCCHHHHHHCCC	9.11	26824392
54	Acetylation	LELRDNDKTRFMGKG HHCCCCCCCCCCCCC	48.10	23236377
54	Ubiquitination	LELRDNDKTRFMGKG HHCCCCCCCCCCCCC	48.10	22790023
55	Phosphorylation	ELRDNDKTRFMGKGV HCCCCCCCCCCCCCH	31.81	25367039
60	Acetylation	DKTRFMGKGVSQAVE CCCCCCCCCHHHHHH	44.33	23806337
60	Malonylation	DKTRFMGKGVSQAVE CCCCCCCCCHHHHHH	44.33	26320211
60	Succinylation	DKTRFMGKGVSQAVE CCCCCCCCCHHHHHH	44.33	-
60	Succinylation	DKTRFMGKGVSQAVE CCCCCCCCCHHHHHH	44.33	23806337
60	Ubiquitination	DKTRFMGKGVSQAVE CCCCCCCCCHHHHHH	44.33	-
63	Phosphorylation	RFMGKGVSQAVEHIN CCCCCCHHHHHHHHH	22.53	22324799
71	Acetylation	QAVEHINKTIAPALV HHHHHHHHCHHHHHH	40.48	23954790
71	Malonylation	QAVEHINKTIAPALV HHHHHHHHCHHHHHH	40.48	26320211
71	Ubiquitination	QAVEHINKTIAPALV HHHHHHHHCHHHHHH	40.48	-
72	Phosphorylation	AVEHINKTIAPALVS HHHHHHHCHHHHHHC	20.20	22324799
79	Phosphorylation	TIAPALVSKKVNVVE CHHHHHHCCCCCEEE	27.84	25521595
80	Acetylation	IAPALVSKKVNVVEQ HHHHHHCCCCCEEEH	53.94	23864654
80	Malonylation	IAPALVSKKVNVVEQ HHHHHHCCCCCEEEH	53.94	26320211
80	Ubiquitination	IAPALVSKKVNVVEQ HHHHHHCCCCCEEEH	53.94	-
81	Acetylation	APALVSKKVNVVEQE HHHHHCCCCCEEEHH	31.38	7743707
81	Malonylation	APALVSKKVNVVEQE HHHHHCCCCCEEEHH	31.38	26320211
81	Ubiquitination	APALVSKKVNVVEQE HHHHHCCCCCEEEHH	31.38	-
89	Acetylation	VNVVEQEKIDKLMIE CCEEEHHHHHHHEEE	56.77	23806337
89	Malonylation	VNVVEQEKIDKLMIE CCEEEHHHHHHHEEE	56.77	26320211
89	Succinylation	VNVVEQEKIDKLMIE CCEEEHHHHHHHEEE	56.77	-
89	Succinylation	VNVVEQEKIDKLMIE CCEEEHHHHHHHEEE	56.77	23806337
89	Ubiquitination	VNVVEQEKIDKLMIE CCEEEHHHHHHHEEE	56.77	-
92	Acetylation	VEQEKIDKLMIEMDG EEHHHHHHHEEECCC	43.35	23806337
92	Malonylation	VEQEKIDKLMIEMDG EEHHHHHHHEEECCC	43.35	26320211
92	Ubiquitination	VEQEKIDKLMIEMDG EEHHHHHHHEEECCC	43.35	-
103	Acetylation	EMDGTENKSKFGANA ECCCCCCCHHHCHHH	49.11	23806337
103	Succinylation	EMDGTENKSKFGANA ECCCCCCCHHHCHHH	49.11	23806337
103	Ubiquitination	EMDGTENKSKFGANA ECCCCCCCHHHCHHH	49.11	-
105	Acetylation	DGTENKSKFGANAIL CCCCCCHHHCHHHHH	49.80	22826441
119	S-nitrosocysteine	LGVSLAVCKAGAVEK HHHHHHHHHHCHHHC	1.76	-
119	Glutathionylation	LGVSLAVCKAGAVEK HHHHHHHHHHCHHHC	1.76	24333276
119	S-nitrosylation	LGVSLAVCKAGAVEK HHHHHHHHHHCHHHC	1.76	24895380
126	Acetylation	CKAGAVEKGVPLYRH HHHCHHHCCCCCHHH	59.62	23806337
126	Malonylation	CKAGAVEKGVPLYRH HHHCHHHCCCCCHHH	59.62	26320211
131	Phosphorylation	VEKGVPLYRHIADLA HHCCCCCHHHHHHHC	8.28	19854140
157	Phosphorylation	FNVINGGSHAGNKLA EEEECCCCCCCCCHH	16.15	26745281
162	Acetylation	GGSHAGNKLAMQEFM CCCCCCCCHHCCEEE	36.38	23954790
176	Phosphorylation	MILPVGASSFREAMR EEECCCHHHHHHHHH	24.87	26643407
177	Phosphorylation	ILPVGASSFREAMRI EECCCHHHHHHHHHH	27.99	26643407
189	Phosphorylation	MRIGAEVYHNLKNVI HHHCHHHHHHHHHHH	4.05	22817900
193	Acetylation	AEVYHNLKNVIKEKY HHHHHHHHHHHHHHH	55.26	23806337
193	Malonylation	AEVYHNLKNVIKEKY HHHHHHHHHHHHHHH	55.26	26320211
193	Ubiquitination	AEVYHNLKNVIKEKY HHHHHHHHHHHHHHH	55.26	-
197	Acetylation	HNLKNVIKEKYGKDA HHHHHHHHHHHCCCC	43.82	132883
199	Acetylation	LKNVIKEKYGKDATN HHHHHHHHHCCCCCC	55.06	23201123
202	Acetylation	VIKEKYGKDATNVGD HHHHHHCCCCCCCCC	40.89	23806337
202	Malonylation	VIKEKYGKDATNVGD HHHHHHCCCCCCCCC	40.89	26320211
202	Ubiquitination	VIKEKYGKDATNVGD HHHHHHCCCCCCCCC	40.89	-
205	Phosphorylation	EKYGKDATNVGDEGG HHHCCCCCCCCCCCC	40.34	23984901
221	Acetylation	APNILENKEALELLK CCCHHCCHHHHHHHH	34.68	22733758
221	Succinylation	APNILENKEALELLK CCCHHCCHHHHHHHH	34.68	23954790
221	Ubiquitination	APNILENKEALELLK CCCHHCCHHHHHHHH	34.68	-
228	Acetylation	KEALELLKTAIAKAG HHHHHHHHHHHHHCC	48.84	23806337
228	Other	KEALELLKTAIAKAG HHHHHHHHHHHHHCC	48.84	-
228	Succinylation	KEALELLKTAIAKAG HHHHHHHHHHHHHCC	48.84	-
228	Succinylation	KEALELLKTAIAKAG HHHHHHHHHHHHHCC	48.84	23806337
228	Ubiquitination	KEALELLKTAIAKAG HHHHHHHHHHHHHCC	48.84	-
229	Phosphorylation	EALELLKTAIAKAGY HHHHHHHHHHHHCCC	24.22	22210690
233	N6-malonyllysine	LLKTAIAKAGYTDQV HHHHHHHHCCCCCEE	35.91	-
233	Acetylation	LLKTAIAKAGYTDQV HHHHHHHHCCCCCEE	35.91	-
233	Malonylation	LLKTAIAKAGYTDQV HHHHHHHHCCCCCEE	35.91	-
254	Phosphorylation	AASEFYRSGKYDLDF HHHHHHHCCCCCCCC	28.40	24925903
256	Acetylation	SEFYRSGKYDLDFKS HHHHHCCCCCCCCCC	36.52	23806337
256	Malonylation	SEFYRSGKYDLDFKS HHHHHCCCCCCCCCC	36.52	26320211
256	Succinylation	SEFYRSGKYDLDFKS HHHHHCCCCCCCCCC	36.52	-
256	Ubiquitination	SEFYRSGKYDLDFKS HHHHHCCCCCCCCCC	36.52	-
257	Phosphorylation	EFYRSGKYDLDFKSP HHHHCCCCCCCCCCC	25.84	24925903
262	Acetylation	GKYDLDFKSPDDPSR CCCCCCCCCCCCHHH	61.82	23806337
262	Succinylation	GKYDLDFKSPDDPSR CCCCCCCCCCCCHHH	61.82	23954790
263	Phosphorylation	KYDLDFKSPDDPSRY CCCCCCCCCCCHHHC	33.29	25521595
268	Phosphorylation	FKSPDDPSRYITPDQ CCCCCCHHHCCCHHH	44.72	25521595
270	Phosphorylation	SPDDPSRYITPDQLA CCCCHHHCCCHHHHH	17.31	25521595
272	Phosphorylation	DDPSRYITPDQLADL CCHHHCCCHHHHHHH	16.33	27180971
280	Phosphorylation	PDQLADLYKSFVQNY HHHHHHHHHHHHHHC	12.72	25777480
281	Acetylation	DQLADLYKSFVQNYP HHHHHHHHHHHHHCC	44.72	-
281	Other	DQLADLYKSFVQNYP HHHHHHHHHHHHHCC	44.72	-
282	Phosphorylation	QLADLYKSFVQNYPV HHHHHHHHHHHHCCE	19.66	26745281
287	Phosphorylation	YKSFVQNYPVVSIED HHHHHHHCCEEEECC	4.74	26745281
291	Phosphorylation	VQNYPVVSIEDPFDQ HHHCCEEEECCCCCC	22.09	-
308	Phosphorylation	WGAWQKFTASAGIQV CCHHHHHHHCCCCEE	27.09	20415495
310	Phosphorylation	AWQKFTASAGIQVVG HHHHHHHCCCCEEEC	24.78	25521595
321	Phosphorylation	QVVGDDLTVTNPKRI EEECCCCEECCHHHH	32.07	20415495
326	Acetylation	DLTVTNPKRIAKAAS CCEECCHHHHHHHCC	59.51	129405
326	Ubiquitination	DLTVTNPKRIAKAAS CCEECCHHHHHHHCC	59.51	22790023
333	Phosphorylation	KRIAKAASEKSCNCL HHHHHHCCCCCCCEE	50.87	25266776
335	Acetylation	IAKAASEKSCNCLLL HHHHCCCCCCCEEEE	58.79	23806337
335	Malonylation	IAKAASEKSCNCLLL HHHHCCCCCCCEEEE	58.79	26320211
335	Succinylation	IAKAASEKSCNCLLL HHHHCCCCCCCEEEE	58.79	-
335	Ubiquitination	IAKAASEKSCNCLLL HHHHCCCCCCCEEEE	58.79	-
336	Phosphorylation	AKAASEKSCNCLLLK HHHCCCCCCCEEEEE	13.40	23649490
337	Glutathionylation	KAASEKSCNCLLLKV HHCCCCCCCEEEEEH	6.54	24333276
337	S-nitrosylation	KAASEKSCNCLLLKV HHCCCCCCCEEEEEH	6.54	24895380
339	Glutathionylation	ASEKSCNCLLLKVNQ CCCCCCCEEEEEHHH	3.12	24333276
339	S-nitrosylation	ASEKSCNCLLLKVNQ CCCCCCCEEEEEHHH	3.12	24895380
343	Acetylation	SCNCLLLKVNQIGSV CCCEEEEEHHHHCHH	38.82	23806337
343	Phosphoglycerylation	SCNCLLLKVNQIGSV CCCEEEEEHHHHCHH	38.82	-
343	Ubiquitination	SCNCLLLKVNQIGSV CCCEEEEEHHHHCHH	38.82	-
349	Phosphorylation	LKVNQIGSVTESLQA EEHHHHCHHHHHHHH	27.74	21743459
351	Phosphorylation	VNQIGSVTESLQACK HHHHCHHHHHHHHHH	22.89	26643407
353	Phosphorylation	QIGSVTESLQACKLA HHCHHHHHHHHHHHH	19.20	26643407
357	S-nitrosocysteine	VTESLQACKLAQSNG HHHHHHHHHHHHHCC	2.09	-
357	Glutathionylation	VTESLQACKLAQSNG HHHHHHHHHHHHHCC	2.09	24333276
357	S-nitrosylation	VTESLQACKLAQSNG HHHHHHHHHHHHHCC	2.09	24895380
357	S-palmitoylation	VTESLQACKLAQSNG HHHHHHHHHHHHHCC	2.09	28526873
358	Acetylation	TESLQACKLAQSNGW HHHHHHHHHHHHCCC	50.28	22826441
362	Phosphorylation	QACKLAQSNGWGVMV HHHHHHHHCCCEEEE	31.53	26745281
370	Phosphorylation	NGWGVMVSHRSGETE CCCEEEEECCCCCCC	8.62	28638064
373	Phosphorylation	GVMVSHRSGETEDTF EEEEECCCCCCCCCH	35.24	22210690
376	Phosphorylation	VSHRSGETEDTFIAD EECCCCCCCCCHHHH	42.56	22210690
379	Phosphorylation	RSGETEDTFIADLVV CCCCCCCCHHHHHHH	15.65	28464351
389	S-nitrosocysteine	ADLVVGLCTGQIKTG HHHHHHHHCCCCCCC	3.10	-
389	Glutathionylation	ADLVVGLCTGQIKTG HHHHHHHHCCCCCCC	3.10	24333276
389	S-nitrosylation	ADLVVGLCTGQIKTG HHHHHHHHCCCCCCC	3.10	24895380
389	S-palmitoylation	ADLVVGLCTGQIKTG HHHHHHHHCCCCCCC	3.10	28526873
394	Ubiquitination	GLCTGQIKTGAPCRS HHHCCCCCCCCCCCH	32.78	-
399	S-nitrosocysteine	QIKTGAPCRSERLAK CCCCCCCCCHHHHHH	8.08	-
399	Glutathionylation	QIKTGAPCRSERLAK CCCCCCCCCHHHHHH	8.08	24333276
399	S-nitrosylation	QIKTGAPCRSERLAK CCCCCCCCCHHHHHH	8.08	21278135
406	Acetylation	CRSERLAKYNQILRI CCHHHHHHHHHHHHH	49.69	23806337
406	Malonylation	CRSERLAKYNQILRI CCHHHHHHHHHHHHH	49.69	26320211
406	Ubiquitination	CRSERLAKYNQILRI CCHHHHHHHHHHHHH	49.69	-
419	Phosphorylation	RIEEELGSKAKFAGR HHHHHHHHCHHHCCC	41.90	25521595
420	N6-malonyllysine	IEEELGSKAKFAGRS HHHHHHHCHHHCCCC	54.52	-
420	Acetylation	IEEELGSKAKFAGRS HHHHHHHCHHHCCCC	54.52	23806337
420	Glutarylation	IEEELGSKAKFAGRS HHHHHHHCHHHCCCC	54.52	24703693
420	Malonylation	IEEELGSKAKFAGRS HHHHHHHCHHHCCCC	54.52	26073543
420	Succinylation	IEEELGSKAKFAGRS HHHHHHHCHHHCCCC	54.52	23806337
420	Ubiquitination	IEEELGSKAKFAGRS HHHHHHHCHHHCCCC	54.52	-
426	Methylation	SKAKFAGRSFRNPLA HCHHHCCCCCCCCCC	29.23	-
427	Phosphorylation	KAKFAGRSFRNPLAK CHHHCCCCCCCCCCC	27.86	26824392

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of ENOA_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ENOA_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ENOA_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of ENOA_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ENOA_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-263, AND MASSSPECTROMETRY.	17114649 [Abstract]
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-44, AND MASSSPECTROMETRY.	18034455 [Abstract]