dbPTM

KPYM_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	KPYM_MOUSE
UniProt AC	P52480
Protein Name	Pyruvate kinase PKM
Gene Name	Pkm
Organism	Mus musculus (Mouse).
Sequence Length	531
Subcellular Localization	Cytoplasm . Nucleus . Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
Protein Description	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation (By similarity)..
Protein Sequence	MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	"N6,N6,N6-trimethyllysine"	-----MPKPHSEAGT -----CCCCCCCCCC	54.81	-
3	Methylation	-----MPKPHSEAGT -----CCCCCCCCCC	54.81	-
3	Ubiquitination	-----MPKPHSEAGT -----CCCCCCCCCC	54.81	22790023
3 (in isoform 2)	Ubiquitination	-	54.81	22790023
6	Phosphorylation	--MPKPHSEAGTAFI --CCCCCCCCCCCHH	37.35	23649490
10	Phosphorylation	KPHSEAGTAFIQTQQ CCCCCCCCCHHHHHH	25.99	23649490
25	Phosphorylation	LHAAMADTFLEHMCR HHHHHHHHHHHHHHC	22.15	29514104
31	S-nitrosocysteine	DTFLEHMCRLDIDSA HHHHHHHHCCCCCCC	4.16	-
31	S-nitrosylation	DTFLEHMCRLDIDSA HHHHHHHHCCCCCCC	4.16	21278135
37	Phosphorylation	MCRLDIDSAPITARN HHCCCCCCCCCEECC	35.96	26824392
41	Phosphorylation	DIDSAPITARNTGII CCCCCCCEECCCEEE	20.64	22817900
49	S-nitrosocysteine	ARNTGIICTIGPASR ECCCEEEEEECCCHH	1.83	-
49	Glutathionylation	ARNTGIICTIGPASR ECCCEEEEEECCCHH	1.83	24333276
49	S-nitrosylation	ARNTGIICTIGPASR ECCCEEEEEECCCHH	1.83	24895380
49	S-palmitoylation	ARNTGIICTIGPASR ECCCEEEEEECCCHH	1.83	28526873
50	Phosphorylation	RNTGIICTIGPASRS CCCEEEEEECCCHHH	20.68	25266776
55	Phosphorylation	ICTIGPASRSVEMLK EEEECCCHHHHHHHH	28.37	26745281
57	Phosphorylation	TIGPASRSVEMLKEM EECCCHHHHHHHHHH	21.67	25266776
62	Acetylation	SRSVEMLKEMIKSGM HHHHHHHHHHHHCCC	43.88	23806337
62	Succinylation	SRSVEMLKEMIKSGM HHHHHHHHHHHHCCC	43.88	23806337
62	Ubiquitination	SRSVEMLKEMIKSGM HHHHHHHHHHHHCCC	43.88	27667366
62 (in isoform 2)	Ubiquitination	-	43.88	22790023
66	Acetylation	EMLKEMIKSGMNVAR HHHHHHHHCCCCEEE	39.38	23806337
66	Succinylation	EMLKEMIKSGMNVAR HHHHHHHHCCCCEEE	39.38	-
66	Succinylation	EMLKEMIKSGMNVAR HHHHHHHHCCCCEEE	39.38	23806337
66	Ubiquitination	EMLKEMIKSGMNVAR HHHHHHHHCCCCEEE	39.38	27667366
66 (in isoform 2)	Ubiquitination	-	39.38	22790023
67	Phosphorylation	MLKEMIKSGMNVARL HHHHHHHCCCCEEEE	32.14	22210690
77	Phosphorylation	NVARLNFSHGTHEYH CEEEEECCCCCCCHH	21.67	27742792
80	Phosphorylation	RLNFSHGTHEYHAET EEECCCCCCCHHHHH	13.20	22499769
83	Phosphorylation	FSHGTHEYHAETIKN CCCCCCCHHHHHHHH	9.95	22499769
87	Phosphorylation	THEYHAETIKNVREA CCCHHHHHHHHHHHH	37.73	22499769
89	Acetylation	EYHAETIKNVREATE CHHHHHHHHHHHHHH	57.73	22826441
89	Ubiquitination	EYHAETIKNVREATE CHHHHHHHHHHHHHH	57.73	22790023
89 (in isoform 2)	Ubiquitination	-	57.73	22790023
95	Phosphorylation	IKNVREATESFASDP HHHHHHHHHHHCCCC	27.66	22499769
97	Phosphorylation	NVREATESFASDPIL HHHHHHHHHCCCCCC	23.04	22499769
100	Phosphorylation	EATESFASDPILYRP HHHHHHCCCCCCCCE	41.74	26824392
100	Ubiquitination	EATESFASDPILYRP HHHHHHCCCCCCCCE	41.74	27667366
105	Phosphorylation	FASDPILYRPVAVAL HCCCCCCCCEEEEEE	17.37	16873679
114	Phosphorylation	PVAVALDTKGPEIRT EEEEEECCCCCCCCC	38.46	22499769
115	Ubiquitination	VAVALDTKGPEIRTG EEEEECCCCCCCCCE	72.62	22790023
115 (in isoform 2)	Ubiquitination	-	72.62	22790023
120	Ubiquitination	DTKGPEIRTGLIKGS CCCCCCCCCEEECCC	22.53	27667366
121	Phosphorylation	TKGPEIRTGLIKGSG CCCCCCCCEEECCCC	41.84	28464351
125	Ubiquitination	EIRTGLIKGSGTAEV CCCCEEECCCCEEEE	52.66	22790023
125 (in isoform 2)	Ubiquitination	-	52.66	22790023
127	Phosphorylation	RTGLIKGSGTAEVEL CCEEECCCCEEEEEE	28.15	22324799
129	Phosphorylation	GLIKGSGTAEVELKK EEECCCCEEEEEECC	22.72	28542873
129	Ubiquitination	GLIKGSGTAEVELKK EEECCCCEEEEEECC	22.72	27667366
135	Acetylation	GTAEVELKKGATLKI CEEEEEECCCCEEEE	35.34	23806337
135	Ubiquitination	GTAEVELKKGATLKI CEEEEEECCCCEEEE	35.34	22790023
135 (in isoform 2)	Ubiquitination	-	35.34	22790023
141	Ubiquitination	LKKGATLKITLDNAY ECCCCEEEEEECCHH	29.32	22790023
141 (in isoform 2)	Ubiquitination	-	29.32	22790023
143	Phosphorylation	KGATLKITLDNAYME CCCEEEEEECCHHHH	26.86	22210690
148	Phosphorylation	KITLDNAYMEKCDEN EEEECCHHHHHCCCC	16.44	28542873
151	Ubiquitination	LDNAYMEKCDENILW ECCHHHHHCCCCEEE	30.38	-
152	S-nitrosocysteine	DNAYMEKCDENILWL CCHHHHHCCCCEEEE	5.17	-
152	Glutathionylation	DNAYMEKCDENILWL CCHHHHHCCCCEEEE	5.17	24333276
152	S-nitrosylation	DNAYMEKCDENILWL CCHHHHHCCCCEEEE	5.17	24895380
162	Ubiquitination	NILWLDYKNICKVVE CEEEEEHHCCEEEEE	39.23	22790023
162 (in isoform 2)	Ubiquitination	-	39.23	22790023
165	S-nitrosocysteine	WLDYKNICKVVEVGS EEEHHCCEEEEEECC	3.70	-
165	Glutathionylation	WLDYKNICKVVEVGS EEEHHCCEEEEEECC	3.70	24333276
165	S-nitrosylation	WLDYKNICKVVEVGS EEEHHCCEEEEEECC	3.70	21278135
166	Acetylation	LDYKNICKVVEVGSK EEHHCCEEEEEECCE	45.58	23806337
166	Succinylation	LDYKNICKVVEVGSK EEHHCCEEEEEECCE	45.58	-
166	Succinylation	LDYKNICKVVEVGSK EEHHCCEEEEEECCE	45.58	23806337
166	Ubiquitination	LDYKNICKVVEVGSK EEHHCCEEEEEECCE	45.58	-
166 (in isoform 2)	Ubiquitination	-	45.58	22790023
175	Phosphorylation	VEVGSKIYVDDGLIS EEECCEEEEECCEEE	11.10	26745281
178	Ubiquitination	GSKIYVDDGLISLQV CCEEEEECCEEEEEE	43.69	27667366
182	Phosphorylation	YVDDGLISLQVKEKG EEECCEEEEEEEHHC	20.14	26643407
186	Ubiquitination	GLISLQVKEKGADFL CEEEEEEEHHCCCEE	40.52	22790023
186 (in isoform 2)	Ubiquitination	-	40.52	22790023
188	Acetylation	ISLQVKEKGADFLVT EEEEEEHHCCCEEEE	54.39	23236377
188	Ubiquitination	ISLQVKEKGADFLVT EEEEEEHHCCCEEEE	54.39	22790023
188 (in isoform 2)	Ubiquitination	-	54.39	22790023
195	Phosphorylation	KGADFLVTEVENGGS HCCCEEEEEEECCCC	36.14	26525534
198	Ubiquitination	DFLVTEVENGGSLGS CEEEEEEECCCCCCC	43.88	27667366
202	Phosphorylation	TEVENGGSLGSKKGV EEEECCCCCCCCCCC	30.84	25521595
205	Phosphorylation	ENGGSLGSKKGVNLP ECCCCCCCCCCCCCC	36.17	20415495
206	Acetylation	NGGSLGSKKGVNLPG CCCCCCCCCCCCCCC	52.31	23236377
206	Ubiquitination	NGGSLGSKKGVNLPG CCCCCCCCCCCCCCC	52.31	22790023
206 (in isoform 2)	Ubiquitination	-	52.31	22790023
207	Acetylation	GGSLGSKKGVNLPGA CCCCCCCCCCCCCCC	70.55	23806337
207	Succinylation	GGSLGSKKGVNLPGA CCCCCCCCCCCCCCC	70.55	23806337
207	Ubiquitination	GGSLGSKKGVNLPGA CCCCCCCCCCCCCCC	70.55	-
207 (in isoform 2)	Ubiquitination	-	70.55	22790023
222	Phosphorylation	AVDLPAVSEKDIQDL CCCCCCCCHHHHHHH	40.82	26525534
224	Acetylation	DLPAVSEKDIQDLKF CCCCCCHHHHHHHCC	53.26	23236377
241	Ubiquitination	EQDVDMVFASFIRKA HHCHHHHHHHHHHHH	3.65	27667366
243	Phosphorylation	DVDMVFASFIRKAAD CHHHHHHHHHHHHHC	15.11	23984901
246	Ubiquitination	MVFASFIRKAADVHE HHHHHHHHHHHCHHH	22.13	27667366
251	Ubiquitination	FIRKAADVHEVRKVL HHHHHHCHHHHHHHH	3.39	27667366
255	Ubiquitination	AADVHEVRKVLGEKG HHCHHHHHHHHCCCC	22.00	27667366
256	Ubiquitination	ADVHEVRKVLGEKGK HCHHHHHHHHCCCCC	47.16	27667366
261	Ubiquitination	VRKVLGEKGKNIKII HHHHHCCCCCCCEEE	73.68	27667366
266	Acetylation	GEKGKNIKIISKIEN CCCCCCCEEEEEECC	43.30	22826441
266	Ubiquitination	GEKGKNIKIISKIEN CCCCCCCEEEEEECC	43.30	27667366
270	Acetylation	KNIKIISKIENHEGV CCCEEEEEECCCHHH	43.56	23806337
270	Ubiquitination	KNIKIISKIENHEGV CCCEEEEEECCCHHH	43.56	27667366
290	Ubiquitination	ILEASDGIMVARGDL HHHHCCCEEEEECCC	2.11	27667366
305	Acetylation	GIEIPAEKVFLAQKM CCCCCHHHHHHHHHH	40.15	23236377
305	Ubiquitination	GIEIPAEKVFLAQKM CCCCCHHHHHHHHHH	40.15	22790023
305 (in isoform 2)	Ubiquitination	-	40.15	22790023
311	Acetylation	EKVFLAQKMMIGRCN HHHHHHHHHHHCCCC	25.48	23806337
311	Succinylation	EKVFLAQKMMIGRCN HHHHHHHHHHHCCCC	25.48	23806337
311	Ubiquitination	EKVFLAQKMMIGRCN HHHHHHHHHHHCCCC	25.48	-
311 (in isoform 2)	Ubiquitination	-	25.48	22790023
317	Glutathionylation	QKMMIGRCNRAGKPV HHHHHCCCCCCCCCE	3.22	24333276
319	Ubiquitination	MMIGRCNRAGKPVIC HHHCCCCCCCCCEEE	48.63	27667366
322	Acetylation	GRCNRAGKPVICATQ CCCCCCCCCEEEEHH	34.82	23806337
322	Succinylation	GRCNRAGKPVICATQ CCCCCCCCCEEEEHH	34.82	-
322	Succinylation	GRCNRAGKPVICATQ CCCCCCCCCEEEEHH	34.82	23806337
324	Ubiquitination	CNRAGKPVICATQML CCCCCCCEEEEHHHH	6.81	27667366
326	S-nitrosocysteine	RAGKPVICATQMLES CCCCCEEEEHHHHHH	3.23	-
326	Glutathionylation	RAGKPVICATQMLES CCCCCEEEEHHHHHH	3.23	24333276
326	S-nitrosylation	RAGKPVICATQMLES CCCCCEEEEHHHHHH	3.23	21278135
328	Phosphorylation	GKPVICATQMLESMI CCCEEEEHHHHHHHH	15.31	29899451
329	Ubiquitination	KPVICATQMLESMIK CCEEEEHHHHHHHHC	18.88	27667366
333	Phosphorylation	CATQMLESMIKKPRP EEHHHHHHHHCCCCC	23.11	29899451
333	Ubiquitination	CATQMLESMIKKPRP EEHHHHHHHHCCCCC	23.11	27667366
346	Phosphorylation	RPTRAEGSDVANAVL CCCCCCCCCHHHHHH	22.03	26239621
358	S-nitrosocysteine	AVLDGADCIMLSGET HHHCCCCEEEEECCC	1.63	-
358	Glutathionylation	AVLDGADCIMLSGET HHHCCCCEEEEECCC	1.63	24333276
358	S-nitrosylation	AVLDGADCIMLSGET HHHCCCCEEEEECCC	1.63	21278135
362	Phosphorylation	GADCIMLSGETAKGD CCCEEEEECCCCCCC	19.73	26239621
365	Phosphorylation	CIMLSGETAKGDYPL EEEEECCCCCCCCCH	36.88	25367039
368	Ubiquitination	LSGETAKGDYPLEAV EECCCCCCCCCHHHH	36.90	27667366
370	Phosphorylation	GETAKGDYPLEAVRM CCCCCCCCCHHHHHH	20.56	22817900
390	Phosphorylation	REAEAAIYHLQLFEE HHHHHHHHHHHHHHH	7.55	26745281
402 (in isoform 2)	Phosphorylation	-	7.52	28464351
403	Hydroxylation	EELRRLAPITSDPTE HHHHHHCCCCCCHHH	34.67	-
405	Phosphorylation	LRRLAPITSDPTEAA HHHHCCCCCCHHHHH	26.47	23984901
405 (in isoform 2)	Phosphorylation	-	26.47	28464351
406	Phosphorylation	RRLAPITSDPTEAAA HHHCCCCCCHHHHHH	42.17	23984901
408	Hydroxylation	LAPITSDPTEAAAVG HCCCCCCHHHHHHHH	31.72	-
409	Phosphorylation	APITSDPTEAAAVGA CCCCCCHHHHHHHHH	43.15	23984901
423	S-nitrosocysteine	AVEASFKCCSGAIIV HHHHHHEECCCCEEE	1.79	-
423	S-nitrosylation	AVEASFKCCSGAIIV HHHHHHEECCCCEEE	1.79	20925432
424	S-nitrosocysteine	VEASFKCCSGAIIVL HHHHHEECCCCEEEE	4.30	-
424	S-nitrosylation	VEASFKCCSGAIIVL HHHHHEECCCCEEEE	4.30	24926564
432	Phosphorylation	SGAIIVLTKSGRSAH CCCEEEEECCCCCHH	16.57	22006019
433	Acetylation	GAIIVLTKSGRSAHQ CCEEEEECCCCCHHH	46.91	-
434	Phosphorylation	AIIVLTKSGRSAHQV CEEEEECCCCCHHHH	33.92	30635358
434 (in isoform 2)	Phosphorylation	-	33.92	28464351
437	Phosphorylation	VLTKSGRSAHQVARY EEECCCCCHHHHHHH	33.36	25266776
466	Phosphorylation	TARQAHLYRGIFPVL HHHHHHHHCCHHHHH	9.27	25367039
474	S-nitrosocysteine	RGIFPVLCKDAVLNA CCHHHHHCHHHHHHH	3.69	-
474	Glutathionylation	RGIFPVLCKDAVLNA CCHHHHHCHHHHHHH	3.69	24333276
474	S-nitrosylation	RGIFPVLCKDAVLNA CCHHHHHCHHHHHHH	3.69	24895380
475	Acetylation	GIFPVLCKDAVLNAW CHHHHHCHHHHHHHH	44.56	23806337
475	Ubiquitination	GIFPVLCKDAVLNAW CHHHHHCHHHHHHHH	44.56	-
498	Acetylation	NLAMDVGKARGFFKK EEEEHHHHHCCCCCC	34.46	23806337
498	Succinylation	NLAMDVGKARGFFKK EEEEHHHHHCCCCCC	34.46	-
498	Succinylation	NLAMDVGKARGFFKK EEEEHHHHHCCCCCC	34.46	23806337
519	Phosphorylation	LTGWRPGSGFTNTMR ECCCCCCCCCCCCEE	33.16	28066266
522	Phosphorylation	WRPGSGFTNTMRVVP CCCCCCCCCCEEEEE	33.05	23984901
524	Phosphorylation	PGSGFTNTMRVVPVP CCCCCCCCEEEEECC	11.87	23984901

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
105	Y	Phosphorylation	Kinase	FGFR1	P11362	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
305	K	Acetylation		-
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of KPYM_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BRNP1_HUMAN	BRINP1	physical	26496610
FIBG_HUMAN	FGG	physical	26496610
KPYM_HUMAN	PKM	physical	26496610
UBL4A_HUMAN	UBL4A	physical	26496610
GUAA_HUMAN	GMPS	physical	26496610
RBG10_HUMAN	RABGAP1L	physical	26496610
RBG1L_HUMAN	RABGAP1L	physical	26496610
ZFY21_HUMAN	ZFYVE21	physical	26496610

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of KPYM_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-105 AND TYR-148, ANDMASS SPECTROMETRY.	18034455 [Abstract]

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