PPARG_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PPARG_MOUSE
• UniProt AC: P37238
• Protein Name: Peroxisome proliferator-activated receptor gamma
• Gene Name: Pparg
• Organism: Mus musculus (Mouse).
• Sequence Length: 505
• Subcellular Localization: Nucleus . Cytoplasm. Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner (By similarity). NOCT enhances its nuclear translocation..
• Protein Description: Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels. [PubMed: 19041764]
• Protein Sequence: MGETLGDSPVDPEHGAFADALPMSTSQEITMVDTEMPFWPTNFGISSVDLSVMEDHSHSFDIKPFTTVDFSSISAPHYEDIPFTRADPMVADYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNRPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKNIPGFINLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKNLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKVLQKMTDLRQIVTEHVQLLHVIKKTETDMSLHPLLQEIYKDLY

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
46	Phosphorylation	WPTNFGISSVDLSVM CCCCCCCCCCEEEHH	25.37	-
51	Phosphorylation	GISSVDLSVMEDHSH CCCCCEEEHHCCCCC	18.24	-
54 (in isoform 2)	O-linked_Glycosylation	-	50.87	22226965
57	Phosphorylation	LSVMEDHSHSFDIKP EEHHCCCCCCCCCCC	32.90	29895711
63	Sumoylation	HSHSFDIKPFTTVDF CCCCCCCCCCEEECH	35.60	-
77 (in isoform 2)	Sumoylation	-	38.03	-
78	Phosphorylation	SSISAPHYEDIPFTR HHCCCCCCCCCCCCC	17.78	-
84	O-linked_Glycosylation	HYEDIPFTRADPMVA CCCCCCCCCCCCCCC	21.42	22226965
107	Sumoylation	QEYQSAIKVEPASPP HHHHHCCCCCCCCCC	40.02	-
112	Phosphorylation	AIKVEPASPPYYSEK CCCCCCCCCCCCCCC	36.80	8240342
133	Phosphorylation	PHEEPSNSLMAIECR CCCCCCCCEEEEEEE	24.70	-
173	Phosphorylation	TIRLKLIYDRCDLNC HHHHHHHHHCCCCCC	14.05	-
268	Acetylation	ARAILTGKTTDKSPF HHHHHCCCCCCCCCE	42.91	51560799
273	Phosphorylation	TGKTTDKSPFVIYDM CCCCCCCCCEEEEEC	26.78	20651683
293	Acetylation	GEDKIKFKHITPLQE CCCCCCCCCCCCCHH	29.15	51560803
296	Phosphorylation	KIKFKHITPLQEQSK CCCCCCCCCCHHCCH	20.05	-
317	Phosphorylation	FQGCQFRSVEAVQEI HCCCCCCCHHHHHHH	26.12	20139300

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
16	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
21	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
78	Y	Phosphorylation	Kinase	SRC	P12931	PSP
112	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
112	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
112	S	Phosphorylation	Kinase	MAPK	-	Uniprot
133	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
133	S	Phosphorylation	Kinase	MAP2K1	Q02750	GPS
273	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
273	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
296	T	Phosphorylation	Kinase	CDK5	Q00535	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	Ahr	P30561	PMID:31653699

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
112	S	Phosphorylation	Ser-112 phosphorylation levels are reduced by 65% in brown adipose tissue compared to white adipose tissue.	21035761
112	S	Phosphorylation	The phosphorylated Ser-112 form is recognized by PER2 and repressed, dephosphorylation at Ser-112 induces adipogenic activity.	21035761

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PPARG_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RXRA_HUMAN	RXRA	physical	10517671
MED1_HUMAN	MED1	physical	12037571
MEN1_MOUSE	Men1	physical	19596783
HDAC1_MOUSE	Hdac1	physical	16431920
SIR1_MOUSE	Sirt1	physical	15175761
NCOR2_HUMAN	NCOR2	physical	16219912
MED14_MOUSE	Med14	physical	20194623
PRGC1_MOUSE	Ppargc1a	physical	15687259
TGFI1_MOUSE	Tgfb1i1	physical	15687259
TGFI1_HUMAN	TGFB1I1	physical	15687259
SP1_MOUSE	Sp1	physical	11777901
UBP7_MOUSE	Usp7	physical	24072712
NCOA2_MOUSE	Ncoa2	physical	22664386
PIAS2_MOUSE	Pias2	physical	15123625
VIME_MOUSE	Vim	physical	23297177
DESM_MOUSE	Des	physical	23297177
NCOR1_HUMAN	NCOR1	physical	11903058
RXRA_MOUSE	Rxra	physical	22573555
PPM1B_MOUSE	Ppm1b	physical	23320500
ABL1_MOUSE	Abl1	physical	25368164
PRGC1_MOUSE	Ppargc1a	physical	25368164
FBX9_MOUSE	Fbxo9	physical	27197753
SNX19_HUMAN	SNX19	physical	27197753
PLD2_HUMAN	PLD2	physical	27197753
TRADD_HUMAN	TRADD	physical	27197753
ADA22_HUMAN	ADAM22	physical	27197753
ITPA_HUMAN	ITPA	physical	27197753
FBX9_HUMAN	FBXO9	physical	27197753
PDYN_HUMAN	PDYN	physical	27197753
TNF11_HUMAN	TNFSF11	physical	27197753
ACTB_HUMAN	ACTB	physical	27197753
M3K7_HUMAN	MAP3K7	physical	27197753
ZC3H3_HUMAN	ZC3H3	physical	27197753
ACTBL_HUMAN	ACTBL2	physical	27197753
ACTG_HUMAN	ACTG1	physical	27197753
ZRAB3_HUMAN	ZRANB3	physical	27197753
PK1L3_HUMAN	PKD1L3	physical	27197753
DUOX1_HUMAN	DUOX1	physical	27197753
RTCB_HUMAN	RTCB	physical	27197753
TARA_HUMAN	TRIOBP	physical	27197753
LAMC2_HUMAN	LAMC2	physical	27197753
MLH1_HUMAN	MLH1	physical	27197753
5HT3D_HUMAN	HTR3D	physical	27197753
ABCA5_HUMAN	ABCA5	physical	27197753
PPARG_HUMAN	PPARG	physical	27197753
AP3D1_HUMAN	AP3D1	physical	27197753
HECD2_HUMAN	HECTD2	physical	27197753
TRPV6_HUMAN	TRPV6	physical	27197753
DCST1_HUMAN	DCST1	physical	27197753
TOPB1_HUMAN	TOPBP1	physical	27197753
ITA8_HUMAN	ITGA8	physical	27197753
AIFM3_HUMAN	AIFM3	physical	27197753
TITIN_HUMAN	TTN	physical	27197753
ZN404_HUMAN	ZNF404	physical	27197753
KI18B_HUMAN	KIF18B	physical	27197753
TIGD1_HUMAN	TIGD1	physical	27197753
GARL3_HUMAN	GARNL3	physical	27197753
COPRS_HUMAN	COPRS	physical	27197753
HYAS1_HUMAN	HAS1	physical	27197753
SCAPE_HUMAN	SCAPER	physical	27197753
ITA9_HUMAN	ITGA9	physical	27197753
CHD1_HUMAN	CHD1	physical	27197753

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.