dbPTM

DDIT3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	DDIT3_HUMAN
UniProt AC	P35638
Protein Name	DNA damage-inducible transcript 3 protein
Gene Name	DDIT3
Organism	Homo sapiens (Human).
Sequence Length	169
Subcellular Localization	Cytoplasm. Nucleus. Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation.
Protein Description	Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response..
Protein Sequence	MAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQGRTRKRKQSGHSPARAGKQRMKEKEQENERKVAQLAEENERLKQEIERLTREVEATRRALIDRMVNLHQA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
14	Phosphorylation	PFSFGTLSSWELEAW CCCCCCCCCHHHHHH	32.99	22817900
15	Phosphorylation	FSFGTLSSWELEAWY CCCCCCCCHHHHHHH	27.90	22817900
30	Phosphorylation	EDLQEVLSSDENGGT HHHHHHHHCCCCCCE	40.70	22817900
31	Phosphorylation	DLQEVLSSDENGGTY HHHHHHHCCCCCCEE	44.79	22817900
49	Phosphorylation	PGNEEEESKIFTTLD CCCHHHHHCCEECCC	34.04	-
54	Phosphorylation	EESKIFTTLDPASLA HHHCCEECCCHHHHH	20.58	-
64	Phosphorylation	PASLAWLTEEEPEPA HHHHHHHCCCCCCCC	30.56	-
77	Phosphorylation	PAEVTSTSQSPHSPD CCCCCCCCCCCCCCC	28.55	22468782
79	Phosphorylation	EVTSTSQSPHSPDSS CCCCCCCCCCCCCCC	25.09	8650547
82	Phosphorylation	STSQSPHSPDSSQSS CCCCCCCCCCCCCCH	33.51	8650547
88	Phosphorylation	HSPDSSQSSLAQEEE CCCCCCCCHHHHHHH	29.30	22468782

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
14	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
14	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
14	S	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM
14	S	Phosphorylation	Kinase	CK2	-	Uniprot
15	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
15	S	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM
15	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
15	S	Phosphorylation	Kinase	CK2	-	Uniprot
30	S	Phosphorylation	Kinase	PRKAA1	Q13131	GPS
30	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
30	S	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM
30	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
30	S	Phosphorylation	Kinase	CK2	-	Uniprot
31	S	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM
31	S	Phosphorylation	Kinase	CK2	-	Uniprot
31	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
31	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
79	S	Phosphorylation	Kinase	P38-SUBFAMILY	-	GPS
79	S	Phosphorylation	Kinase	P38_GROUP	-	PhosphoELM
79	S	Phosphorylation	Kinase	P38A	Q16539	PSP
82	S	Phosphorylation	Kinase	P38-SUBFAMILY	-	GPS
82	S	Phosphorylation	Kinase	P38_GROUP	-	PhosphoELM
82	S	Phosphorylation	Kinase	P38A	Q16539	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of DDIT3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of DDIT3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DHB14_HUMAN	HSD17B14	physical	16189514
MCMBP_HUMAN	MCMBP	physical	16189514
SPOP_HUMAN	SPOP	physical	16189514
BATF_HUMAN	BATF	physical	16189514
CSK21_HUMAN	CSNK2A1	physical	12876286
CEBPB_HUMAN	CEBPB	physical	12706815
CEBPB_HUMAN	CEBPB	physical	8662954
JUND_HUMAN	JUND	physical	10523647
FOS_HUMAN	FOS	physical	10523647
JUN_HUMAN	JUN	physical	10523647
ATF3_HUMAN	ATF3	physical	8622660
CN080_HUMAN	C14orf80	physical	20562859
RTL6_HUMAN	LDOC1L	physical	20562859
CEBPB_HUMAN	CEBPB	physical	20562859
HXA5_HUMAN	HOXA5	physical	20211142
SSX3_HUMAN	SSX3	physical	20211142
RAI1_HUMAN	RAI1	physical	20211142
ZSC31_HUMAN	ZSCAN31	physical	20211142
HDAC1_HUMAN	HDAC1	physical	22242125
SRA1_HUMAN	SRA1	physical	20398657
CSN8_HUMAN	COPS8	physical	23213463
CUL3_HUMAN	CUL3	physical	23213463
CUL1_HUMAN	CUL1	physical	23213463
CSN3_HUMAN	COPS3	physical	23213463
KEAP1_HUMAN	KEAP1	physical	23213463
BACH2_HUMAN	BACH2	physical	23661758
BACH1_HUMAN	BACH1	physical	23661758
MAFG_HUMAN	MAFG	physical	23661758
MAFF_HUMAN	MAFF	physical	23661758
BATF3_HUMAN	BATF3	physical	23661758
BATF2_HUMAN	BATF2	physical	23661758
BATF_HUMAN	BATF	physical	23661758
ATF3_HUMAN	ATF3	physical	23661758
ATF4_HUMAN	ATF4	physical	23661758
FOSL1_HUMAN	FOSL1	physical	23661758
FOS_HUMAN	FOS	physical	23661758
JUNB_HUMAN	JUNB	physical	23661758
JUN_HUMAN	JUN	physical	23661758
ATF2_HUMAN	ATF2	physical	23661758
EPAS1_HUMAN	EPAS1	physical	23661758
DBP_HUMAN	DBP	physical	23661758
NFIL3_HUMAN	NFIL3	physical	23661758
CR3L1_HUMAN	CREB3L1	physical	23661758
CREB3_HUMAN	CREB3	physical	23661758
DDIT3_HUMAN	DDIT3	physical	23661758
THRB_HUMAN	F2	physical	21988832
GP1BA_HUMAN	GP1BA	physical	21988832
IMA1_HUMAN	KPNA2	physical	21988832
NEMO_HUMAN	IKBKG	physical	21988832
IDD_HUMAN	DGCR2	physical	21988832
PPIB_HUMAN	PPIB	physical	24270407
ATF4_HUMAN	ATF4	physical	18330356
BATF_HUMAN	BATF	physical	18330356
CN080_HUMAN	C14orf80	physical	18330356
GIPC1_HUMAN	GIPC1	physical	18330356
EP300_HUMAN	EP300	physical	17872950
TRIB3_HUMAN	TRIB3	physical	17872950
FOSL2_HUMAN	FOSL2	physical	25416956
RBTN2_HUMAN	LMO2	physical	25416956
ZBT25_HUMAN	ZBTB25	physical	25416956
TNF12_HUMAN	TNFSF12	physical	25416956
SNPC5_HUMAN	SNAPC5	physical	25416956
DHB14_HUMAN	HSD17B14	physical	25416956
AMOL2_HUMAN	AMOTL2	physical	25416956
VP37C_HUMAN	VPS37C	physical	25416956
BATF3_HUMAN	BATF3	physical	25416956
EMSY_HUMAN	C11orf30	physical	25416956
ZC3HE_HUMAN	ZC3H14	physical	25416956
ATPF2_HUMAN	ATPAF2	physical	25416956
CC153_HUMAN	CCDC153	physical	25416956
CBP_HUMAN	CREBBP	physical	20829347
CEBPB_HUMAN	CEBPB	physical	1547942
SPOP_HUMAN	SPOP	physical	24990631
GABR2_HUMAN	GABBR2	physical	16081421
CEBPA_HUMAN	CEBPA	physical	27555288
FANCA_HUMAN	FANCA	physical	28215707
PRKN_HUMAN	PARK2	physical	28522833

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
613488	Myxoid liposarcoma (MXLIPO)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of DDIT3_HUMAN

Related Literatures of Post-Translational Modification