dbPTM

NFE2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NFE2_MOUSE
UniProt AC	Q07279
Protein Name	Transcription factor NF-E2 45 kDa subunit
Gene Name	Nfe2
Organism	Mus musculus (Mouse).
Sequence Length	373
Subcellular Localization	Nucleus, PML body. Cytoplasm. The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs). Translocated to the cytoplasm through interaction with ITCH (By similarity)..
Protein Description	Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron..
Protein Sequence	MPPCPPQQNRNRLSQLPVGELGEMELTWQEIMSITELQGLNVPSETSFEPQAPTPYPGPLPPPTYCPCSIHPDAGFSLPPPSYELPASTPHVPELPYSYGNVAIPVSKPLTLSGLLNEPLPDHLALLDIGLPVGQPKPQEDPESDSGLSLNYSDAESLELEGMEAGRRRSEYVDMYPVEYPYSLMPNSLAHPNYTLPPTETPLALESSSGPVRAKPAVRGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLAQYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLSSERERLLRARGEADRTLEVMRQQLAELYHDIFQHLRDESGNSYSPEEYVLQQAADGAIFLVPRGTKMEATD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference	Orthologous Protein Cluster
157	Phosphorylation	LNYSDAESLELEGME CCCCHHHHHHHCCHH	28.94	19966288
170	Phosphorylation	MEAGRRRSEYVDMYP HHHHCCCHHCCCCEE	31.31	19966288

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
157	S	Phosphorylation	Kinase	MAPK8	Q91Y86	Uniprot
170	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
170	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
170	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NFE2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NFE2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ORC2_MOUSE	Orc2	physical	20211142
MBB1A_MOUSE	Mybbp1a	physical	20211142
KAT2B_MOUSE	Kat2b	physical	20211142
PER1_MOUSE	Per1	physical	20211142
REQU_MOUSE	Dpf2	physical	20211142
KDM5C_MOUSE	Kdm5c	physical	20211142
KDM5D_MOUSE	Kdm5d	physical	20211142
SNAI1_MOUSE	Snai1	physical	20211142
SPT4A_MOUSE	Supt4a	physical	20211142
TIAR_MOUSE	Tial1	physical	20211142
TS101_MOUSE	Tsg101	physical	20211142
UBIP1_MOUSE	Ubp1	physical	20211142
ZSCA2_MOUSE	Zscan2	physical	20211142
ASH2L_MOUSE	Ash2l	physical	20211142
TF2H2_MOUSE	Gtf2h2	physical	20211142
ZN292_MOUSE	Zfp292	physical	20211142
PDLI4_MOUSE	Pdlim4	physical	20211142
PLRG1_MOUSE	Plrg1	physical	20211142
TRIP4_MOUSE	Trip4	physical	20211142
ANR49_MOUSE	Ankrd49	physical	20211142
RNF14_MOUSE	Rnf14	physical	20211142
SMCE1_MOUSE	Smarce1	physical	20211142
SIR2_MOUSE	Sirt2	physical	20211142
ASB1_MOUSE	Asb1	physical	20211142
ZDHC6_MOUSE	Zdhhc6	physical	20211142
T2EB_MOUSE	Gtf2e2	physical	20211142
RPB4_MOUSE	Polr2d	physical	20211142
ASB12_MOUSE	Asb12	physical	20211142
ERCC8_MOUSE	Ercc8	physical	20211142
MMS19_MOUSE	Mms19	physical	20211142
T2EA_MOUSE	Gtf2e1	physical	20211142
PBX4_MOUSE	Pbx4	physical	20211142
SIR1_MOUSE	Sirt1	physical	20211142
CSDC2_MOUSE	Csdc2	physical	20211142
CHD4_MOUSE	Chd4	physical	20211142
MED21_MOUSE	Med21	physical	20211142
TLE6_MOUSE	Tle6	physical	20211142
ASH1L_MOUSE	Ash1l	physical	20211142
KDM4B_MOUSE	Kdm4b	physical	20211142
CSDE1_MOUSE	Csde1	physical	20211142
RPB2_MOUSE	Polr2b	physical	20211142
ZN592_MOUSE	Zfp592	physical	20211142
CARTF_MOUSE	Carf	physical	20211142
HIF1N_MOUSE	Hif1an	physical	20211142
GCFC2_MOUSE	Gcfc2	physical	20211142
MK08_MOUSE	Mapk8	physical	19966288
ASH2L_MOUSE	Ash2l	physical	17707229
RBBP5_MOUSE	Rbbp5	physical	17707229
WDR5_MOUSE	Wdr5	physical	17707229
MEN1_MOUSE	Men1	physical	17707229
MAFK_MOUSE	Mafk	physical	17707229
WWP1_HUMAN	WWP1	physical	9753456

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NFE2_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"JNK-mediated turnover and stabilization of the transcription factorp45/NF-E2 during differentiation of murine erythroleukemia cells."; Lee T.L., Shyu Y.C., Hsu P.H., Chang C.W., Wen S.C., Hsiao W.Y.,Tsai M.D., Shen C.K.; Proc. Natl. Acad. Sci. U.S.A. 107:52-57(2010). Cited for: PHOSPHORYLATION AT SER-157, INTERACTION WITH MAPK8, UBIQUITINATION,MASS SPECTROMETRY, AND MUTAGENESIS OF SER-157.	19966288 [Abstract]
"Regulation of the erythroid transcription factor NF-E2 by cyclicadenosine monophosphate-dependent protein kinase."; Casteel D., Suhasini M., Gudi T., Naima R., Pilz R.B.; Blood 91:3193-3201(1998). Cited for: PHOSPHORYLATION AT SER-170, FUNCTION, AND MUTAGENESIS OF SER-170.	9558374 [Abstract]