SNAI1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SNAI1_MOUSE
• UniProt AC: Q02085
• Protein Name: Zinc finger protein SNAI1
• Gene Name: Snai1
• Organism: Mus musculus (Mouse).
• Sequence Length: 264
• Subcellular Localization: Nucleus . Cytoplasm . Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs..
• Protein Description: Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription. [PubMed: 24239292 SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits]
• Protein Sequence: MPRSFLVRKPSDPRRKPNYSELQDACVEFTFQQPYDQAHLLAAIPPPEVLNPAASLPTLIWDSLLVPQVRPVAWATLPLRESPKAVELTSLSDEDSGKSSQPPSPPSPAPSSFSSTSASSLEAEAFIAFPGLGQLPKQLARLSVAKDPQSRKIFNCKYCNKEYLSLGALKMHIRSHTLPCVCTTCGKAFSRPWLLQGHVRTHTGEKPFSCSHCNRAFADRSNLRAHLQTHSDVKRYQCQACARTFSRMSLLHKHQESGCSGGPR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
11	Phosphorylation	SFLVRKPSDPRRKPN CCCCCCCCCCCCCCC	64.38	-
82	Phosphorylation	ATLPLRESPKAVELT EECCCCCCCCEEEEE	25.85	-
89	Phosphorylation	SPKAVELTSLSDEDS CCCEEEEEECCCCCC	17.70	25619855
90	Phosphorylation	PKAVELTSLSDEDSG CCEEEEEECCCCCCC	38.20	25619855
92	Phosphorylation	AVELTSLSDEDSGKS EEEEEECCCCCCCCC	38.43	25619855
96	Phosphorylation	TSLSDEDSGKSSQPP EECCCCCCCCCCCCC	46.18	12832491
100	Phosphorylation	DEDSGKSSQPPSPPS CCCCCCCCCCCCCCC	50.63	12832491
104	Phosphorylation	GKSSQPPSPPSPAPS CCCCCCCCCCCCCCC	57.01	-
107	Phosphorylation	SQPPSPPSPAPSSFS CCCCCCCCCCCCCCC	36.76	12832491
111	Phosphorylation	SPPSPAPSSFSSTSA CCCCCCCCCCCCCCC	46.23	12832491
112	O-linked_Glycosylation	PPSPAPSSFSSTSAS CCCCCCCCCCCCCCC	28.26	-
115	Phosphorylation	PAPSSFSSTSASSLE CCCCCCCCCCCCHHC	25.11	12832491
119	Phosphorylation	SFSSTSASSLEAEAF CCCCCCCCHHCEEEE	34.91	12832491
143	Phosphorylation	PKQLARLSVAKDPQS HHHHHHHHCCCCCCC	17.93	24719451
203	Phosphorylation	QGHVRTHTGEKPFSC CCCEEECCCCCCCCC	46.56	-
231	Phosphorylation	RAHLQTHSDVKRYQC HHHHHHHHHHHHHHH	47.70	27149854
246	Phosphorylation	QACARTFSRMSLLHK HHHHHHHHHHHHHHH	26.85	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	Btrc	Q3ULA2	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
11	S	Phosphorylation	Phosphorylation at Ser-11 and Ser-104 positively regulates its function in induction of EMT and/or cell survival, respectively.	-
96	S	Phosphorylation	Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96.	-
98	K	ubiquitylation	Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation.	-
100	S	Phosphorylation	Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96.	-
104	S	Phosphorylation	Phosphorylation at Ser-11 and Ser-104 positively regulates its function in induction of EMT and/or cell survival, respectively.	-
104	S	Phosphorylation	Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96.	-
112	S	Phosphorylation	O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.	-
137	K	ubiquitylation	Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation.	-
146	K	ubiquitylation	Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SNAI1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FXL14_HUMAN	FBXL14	physical	19955572
TNAP3_MOUSE	Tnfaip3	physical	28892081
GSK3B_MOUSE	Gsk3b	physical	28892081

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.