NHRF3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NHRF3_HUMAN
• UniProt AC: Q5T2W1
• Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• Gene Name: PDZK1
• Organism: Homo sapiens (Human).
• Sequence Length: 519
• Subcellular Localization: Membrane ; Peripheral membrane protein . Cell membrane . Associated with peripheral membranes. Localizes to the apical compartment of proximal tubular cells and to sinusoidal liver membranes.
• Protein Description: A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. Required for normal cell-surface expression of SCARB1. Plays a role in maintaining normal plasma cholesterol levels via its effects on SCARB1. Plays a role in the normal localization and function of the chloride-anion exchanger SLC26A6 to the plasma membrane in the brush border of the proximal tubule of the kidney. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function (By similarity)..
• Protein Sequence: MTSTFNPRECKLSKQEGQNYGFFLRIEKDTEGHLVRVVEKCSPAEKAGLQDGDRVLRINGVFVDKEEHMQVVDLVRKSGNSVTLLVLDGDSYEKAVKTRVDLKELGQSQKEQGLSDNILSPVMNGGVQTWTQPRLCYLVKEGGSYGFSLKTVQGKKGVYMTDITPQGVAMRAGVLADDHLIEVNGENVEDASHEEVVEKVKKSGSRVMFLLVDKETDKRHVEQKIQFKRETASLKLLPHQPRIVEMKKGSNGYGFYLRAGSEQKGQIIKDIDSGSPAEEAGLKNNDLVVAVNGESVETLDHDSVVEMIRKGGDQTSLLVVDKETDNMYRLAHFSPFLYYQSQELPNGSVKEAPAPTPTSLEVSSPPDTTEEVDHKPKLCRLAKGENGYGFHLNAIRGLPGSFIKEVQKGGPADLAGLEDEDVIIEVNGVNVLDEPYEKVVDRIQSSGKNVTLLVCGKKAYDYFQAKKIPIVSSLADPLDTPPDSKEGIVVESNHDSHMAKERAHSTASHSSSNSEDTEM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MTSTFNPRE ------CCCCCCHHH	36.15	-
78	Phosphorylation	VVDLVRKSGNSVTLL HHEEEECCCCEEEEE	32.42	28857561
81	Phosphorylation	LVRKSGNSVTLLVLD EEECCCCEEEEEEEC	21.72	28857561
83	Phosphorylation	RKSGNSVTLLVLDGD ECCCCEEEEEEECCC	17.75	28857561
108	Phosphorylation	DLKELGQSQKEQGLS CHHHHCHHHHHCCCC	41.23	-
120	Phosphorylation	GLSDNILSPVMNGGV CCCCCCCHHHHCCCC	16.20	28857561
144	Phosphorylation	YLVKEGGSYGFSLKT EEEECCCCCCEEEEE	32.70	28857561
148	Phosphorylation	EGGSYGFSLKTVQGK CCCCCCEEEEEECCC	25.29	28857561
151	Phosphorylation	SYGFSLKTVQGKKGV CCCEEEEEECCCCEE	24.29	28857561
192	Phosphorylation	GENVEDASHEEVVEK CCCCCCCCHHHHHHH	43.69	-
250	Phosphorylation	IVEMKKGSNGYGFYL EEEEECCCCCCCEEE	35.64	28857561
261	Phosphorylation	GFYLRAGSEQKGQII CEEEECCCCCCCEEE	35.55	28857561
316	Phosphorylation	RKGGDQTSLLVVDKE HCCCCCCEEEEEECC	17.67	22210691
328	Phosphorylation	DKETDNMYRLAHFSP ECCCCCCCHHHHCCH	14.55	22210691
334	Phosphorylation	MYRLAHFSPFLYYQS CCHHHHCCHHHEEEC	12.60	-
348	Phosphorylation	SQELPNGSVKEAPAP CCCCCCCCCCCCCCC	36.33	28857561
356	Phosphorylation	VKEAPAPTPTSLEVS CCCCCCCCCCCEEEC	41.11	26657352
358	Phosphorylation	EAPAPTPTSLEVSSP CCCCCCCCCEEECCC	48.71	30278072
359	Phosphorylation	APAPTPTSLEVSSPP CCCCCCCCEEECCCC	24.60	30278072
363	Phosphorylation	TPTSLEVSSPPDTTE CCCCEEECCCCCCCC	27.96	30278072
364	Phosphorylation	PTSLEVSSPPDTTEE CCCEEECCCCCCCCC	45.70	28188228
368	Phosphorylation	EVSSPPDTTEEVDHK EECCCCCCCCCCCCC	41.71	23312004
369	Phosphorylation	VSSPPDTTEEVDHKP ECCCCCCCCCCCCCC	37.21	28857561
401	Phosphorylation	AIRGLPGSFIKEVQK HHCCCCHHHHHHHHH	23.12	24719451
451	Phosphorylation	QSSGKNVTLLVCGKK HHCCCEEEEEEECCC	24.62	28857561
472	Phosphorylation	AKKIPIVSSLADPLD HCCCCEEECCCCCCC	21.98	29978859
473	Phosphorylation	KKIPIVSSLADPLDT CCCCEEECCCCCCCC	19.38	29978859
480	Phosphorylation	SLADPLDTPPDSKEG CCCCCCCCCCCCCCC	44.39	28192239
484	Phosphorylation	PLDTPPDSKEGIVVE CCCCCCCCCCCEEEE	38.08	29978859
492	Phosphorylation	KEGIVVESNHDSHMA CCCEEEECCCCHHHH	28.05	21082442
505	Phosphorylation	MAKERAHSTASHSSS HHHHHHHHHCCCCCC	25.36	28857561
506	Phosphorylation	AKERAHSTASHSSSN HHHHHHHHCCCCCCC	23.47	28857561
508	Phosphorylation	ERAHSTASHSSSNSE HHHHHHCCCCCCCCC	24.80	28857561
510	Phosphorylation	AHSTASHSSSNSEDT HHHHCCCCCCCCCCC	32.73	28857561
511	Phosphorylation	HSTASHSSSNSEDTE HHHCCCCCCCCCCCC	28.28	28857561
512	Phosphorylation	STASHSSSNSEDTEM HHCCCCCCCCCCCCC	47.60	28857561
514	Phosphorylation	ASHSSSNSEDTEM-- CCCCCCCCCCCCC--	38.50	28857561
517	Phosphorylation	SSSNSEDTEM----- CCCCCCCCCC-----	29.87	25072903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
505	S	Phosphorylation	Kinase	PKACA	P17612	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NHRF3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NHRF3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SL9A3_HUMAN	SLC9A3	physical	14531806
NPT1_HUMAN	SLC17A1	physical	14531806
MRP2_HUMAN	ABCC2	physical	10496535
S22A4_HUMAN	SLC22A4	physical	14531806
AKA10_HUMAN	AKAP10	physical	14531806
S22AC_HUMAN	SLC22A12	physical	14531806
PEX5_HUMAN	PEX5	physical	16189514
UBC9_HUMAN	UBE2I	physical	14531806
PDZ1I_HUMAN	PDZK1IP1	physical	14531806
NHRF2_HUMAN	SLC9A3R2	physical	14531806
FARP2_HUMAN	FARP2	physical	14531806
SLK_HUMAN	SLK	physical	14531806
NHRF1_HUMAN	SLC9A3R1	physical	14531806
PDZ1I_HUMAN	PDZK1IP1	physical	12837682
PDZ1I_HUMAN	PDZK1IP1	physical	12754212
SCRB1_HUMAN	SCARB1	physical	12119305
CLCN3_HUMAN	CLCN3	physical	12471024
CFTR_HUMAN	CFTR	physical	12471024
CFTR_HUMAN	CFTR	physical	11051556
KLH17_RAT	Klhl17	physical	12063253
DLG4_RAT	Dlg4	physical	12063253
A4_HUMAN	APP	physical	21832049
MRP4_HUMAN	ABCC4	physical	18045536
PI2R_MOUSE	Ptgir	physical	23457445
CDC37_HUMAN	CDC37	physical	24869908
AKT1_HUMAN	AKT1	physical	24869908
NPT2A_HUMAN	SLC34A1	physical	14531806
MRP2_HUMAN	ABCC2	physical	21059598
RHG17_HUMAN	ARHGAP17	physical	28514442
ASND1_HUMAN	ASNSD1	physical	28514442
MRP4_HUMAN	ABCC4	physical	28514442
BCR_HUMAN	BCR	physical	28514442
FRYL_HUMAN	FRYL	physical	28514442
SHKB1_HUMAN	SHKBP1	physical	28514442
KCTD3_HUMAN	KCTD3	physical	28514442
SH2D5_HUMAN	SH2D5	physical	28514442
KCD17_HUMAN	KCTD17	physical	28514442
SC6A6_HUMAN	SLC6A6	physical	28514442
S22A5_HUMAN	SLC22A5	physical	28514442
ZBED1_HUMAN	ZBED1	physical	28514442
KCTD2_HUMAN	KCTD2	physical	28514442
COL12_HUMAN	COLEC12	physical	28514442
S4A7_HUMAN	SLC4A7	physical	28514442
KCTD5_HUMAN	KCTD5	physical	28514442
KLH17_HUMAN	KLHL17	physical	28514442
SC5A6_HUMAN	SLC5A6	physical	28514442
OTUL_HUMAN	OTULIN	physical	28514442
SCRB1_HUMAN	SCARB1	physical	28514442
ZBTB5_HUMAN	ZBTB5	physical	28514442
S19A3_HUMAN	SLC19A3	physical	28514442
DECR2_HUMAN	DECR2	physical	28514442
KLH24_HUMAN	KLHL24	physical	28514442
ECI2_HUMAN	ECI2	physical	28514442
CK5P1_HUMAN	CDK5RAP1	physical	28514442
EAA1_HUMAN	SLC1A3	physical	28514442
CSK22_HUMAN	CSNK2A2	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.