dbPTM

EAA1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	EAA1_HUMAN
UniProt AC	P43003
Protein Name	Excitatory amino acid transporter 1 {ECO:0000303\|PubMed:16042756, ECO:0000303\|PubMed:8647279}
Gene Name	SLC1A3
Organism	Homo sapiens (Human).
Sequence Length	542
Subcellular Localization	Cell membrane Multi-pass membrane protein .
Protein Description	Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. [PubMed: 7521911]
Protein Sequence	MTKSNGEEPKMGGRMERFQQGVRKRTLLAKKKVQNITKEDVKSYLFRNAFVLLTVTAVIVGTILGFTLRPYRMSYREVKYFSFPGELLMRMLQMLVLPLIISSLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGIIIVIIIHPGKGTKENMHREGKIVRVTAADAFLDLIRNMFPPNLVEACFKQFKTNYEKRSFKVPIQANETLVGAVINNVSEAMETLTRITEELVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNEAIMRLVAVIMWYAPVGILFLIAGKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRVTRFVLPVGATINMDGTALYEALAAIFIAQVNNFELNFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNETEKPIDSETKM

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MTKSNGEEP ------CCCCCCCCC	43.08	24719451
3	Ubiquitination	-----MTKSNGEEPK -----CCCCCCCCCC	40.40	-
4	Phosphorylation	----MTKSNGEEPKM ----CCCCCCCCCCC	40.68	24719451
10	Ubiquitination	KSNGEEPKMGGRMER CCCCCCCCCCCHHHH	54.15	-
26	Phosphorylation	QQGVRKRTLLAKKKV HHHHHHHHHHHHHHH	29.20	22912867
31	Ubiquitination	KRTLLAKKKVQNITK HHHHHHHHHHCCCCH	53.20	-
32	Ubiquitination	RTLLAKKKVQNITKE HHHHHHHHHCCCCHH	48.63	-
37	Phosphorylation	KKKVQNITKEDVKSY HHHHCCCCHHHHHHH	35.61	-
38	Acetylation	KKVQNITKEDVKSYL HHHCCCCHHHHHHHH	48.39	20167786
38	Ubiquitination	KKVQNITKEDVKSYL HHHCCCCHHHHHHHH	48.39	-
67	Phosphorylation	VGTILGFTLRPYRMS HHHHHHCCCCCCCCC	21.76	24719451
79	Ubiquitination	RMSYREVKYFSFPGE CCCCCEECCCCCCHH	35.51	-
82	Phosphorylation	YREVKYFSFPGELLM CCEECCCCCCHHHHH	27.69	-
191	Ubiquitination	EACFKQFKTNYEKRS HHHHHHHHHCCCCCC	33.11	-
223	Phosphorylation	NVSEAMETLTRITEE CHHHHHHHHHHHHHH	21.98	29759185
228	Phosphorylation	METLTRITEELVPVP HHHHHHHHHHHCCCC	21.81	29759185
384	Ubiquitination	EENNGVDKRVTRFVL HHCCCCCCCEEEEEE	46.49	-
482	Phosphorylation	LDRLRTTTNVLGDSL HHHHHHHCCHHCCHH	23.79	12911626
503	Ubiquitination	HLSRHELKNRDVEMG HHHHHHHHCCCCCCC	47.13	-
512	Phosphorylation	RDVEMGNSVIEENEM CCCCCCCCHHCCCCC	20.81	25159151
520	Ubiquitination	VIEENEMKKPYQLIA HHCCCCCCCCEEEEC	44.40	21906983
521	Ubiquitination	IEENEMKKPYQLIAQ HCCCCCCCCEEEECC	47.88	21906983
521	Acetylation	IEENEMKKPYQLIAQ HCCCCCCCCEEEECC	47.88	26051181
523	Phosphorylation	ENEMKKPYQLIAQDN CCCCCCCEEEECCCC	25.49	21945579
532	Phosphorylation	LIAQDNETEKPIDSE EECCCCCCCCCCCCC	56.41	21945579
534	Ubiquitination	AQDNETEKPIDSETK CCCCCCCCCCCCCCC	55.48	21906983
538	Phosphorylation	ETEKPIDSETKM--- CCCCCCCCCCCC---	48.13	21945579
540	Phosphorylation	EKPIDSETKM----- CCCCCCCCCC-----	36.04	21945579
541	Ubiquitination	KPIDSETKM------ CCCCCCCCC------	38.26	21906983

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
26	T	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
37	T	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	NEDD4L	Q96PU5	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of EAA1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of EAA1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of EAA1_HUMAN !!

Drug and Disease Associations

Kegg Disease
H00749	Episodic ataxias
OMIM Disease
612656	Episodic ataxia 6 (EA6)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of EAA1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512, AND MASSSPECTROMETRY.	18669648 [Abstract]