YPK2_YEAST - dbPTM
YPK2_YEAST - PTM Information in dbPTM
Basic Information of Protein
UniProt ID YPK2_YEAST
UniProt AC P18961
Protein Name Serine/threonine-protein kinase YPK2/YKR2
Gene Name YPK2
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length 677
Subcellular Localization Nucleus. Cytoplasm.
Protein Description May act as a downstream kinase in the sphingolipid-mediated signaling pathway. Plays an essential role in the proliferation of yeast cells. Involved in a signaling pathway, required for optimal cell wall integrity, that acts in parallel with the PKC1-SLT2-dependent pathway. A substrate of TOR complex 2 (TORC2) and required for TORC2 to regulate spatial aspects of cell growth. Phosphorylation of residue Thr-501 is indispensable for function..
Protein Sequence MHSWRISKFKLGRSKEDDGSSEDENEKSWGNGLFHFHHGEKHHDGSPKNHNHEHEHHIRKINTNETLPSSLSSPKLRNDASFKNPSGIGNDNSKASERKASQSSTETQGPSSESGLMTVKVYSGKDFTLPFPITSNSTILQKLLSSGILTSSSNDASEVAAIMRQLPRYKRVDQDSAGEGLIDRAFATKFIPSSILLPGSTNSSPLLYFTIEFDNSITTISPDMGTMEQPVFNKISTFDVTRKLRFLKIDVFARIPSLLLPSKNWQQEIGEQDEVLKEILKKINTNQDIHLDSFHLPLNLKIDSAAQIRLYNHHWISLERGYGKLNITVDYKPSKNKPLSIDDFDLLKVIGKGSFGKVMQVRKKDTQKIYALKALRKAYIVSKCEVTHTLAERTVLARVDCPFIVPLKFSFQSPEKLYLVLAFINGGELFYHLQHEGRFSLARSRFYIAELLCALDSLHKLDVIYRDLKPENILLDYQGHIALCDFGLCKLNMKDNDKTDTFCGTPEYLAPEILLGQGYTKTVDWWTLGILLYEMMTGLPPYYDENVPVMYKKILQQPLLFPDGFDPAAKDLLIGLLSRDPSRRLGVNGTDEIRNHPFFKDISWKKLLLKGYIPPYKPIVKSEIDTANFDQEFTKEKPIDSVVDEYLSASIQKQFGGWTYIGDEQLGDSPSQGRSIS
Overview of Protein Modification Sites with Functional and Structural Information
Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations Modification Substrate Peptides
&
Secondary Structure		 ASA (%) Reference Orthologous
Protein Cluster
20PhosphorylationRSKEDDGSSEDENEK
CCCCCCCCCCCCCCC		37.2328889911
21PhosphorylationSKEDDGSSEDENEKS
CCCCCCCCCCCCCCC		55.3824603354
46PhosphorylationGEKHHDGSPKNHNHE
CCCCCCCCCCCCCCC		38.4628889911
60UbiquitinationEHEHHIRKINTNETL
CCHHHEEECCCCCCC		39.4417644757
63PhosphorylationHHIRKINTNETLPSS
HHEEECCCCCCCCCC		37.5919823750
66PhosphorylationRKINTNETLPSSLSS
EECCCCCCCCCCCCC		46.1421551504
69PhosphorylationNTNETLPSSLSSPKL
CCCCCCCCCCCCCCC		47.2020377248
70PhosphorylationTNETLPSSLSSPKLR
CCCCCCCCCCCCCCC		30.2620377248
72PhosphorylationETLPSSLSSPKLRND
CCCCCCCCCCCCCCC		46.6821551504
73PhosphorylationTLPSSLSSPKLRNDA
CCCCCCCCCCCCCCC		31.2029136822
75UbiquitinationPSSLSSPKLRNDASF
CCCCCCCCCCCCCCC		63.1217644757
81PhosphorylationPKLRNDASFKNPSGI
CCCCCCCCCCCCCCC		39.3529136822
83AcetylationLRNDASFKNPSGIGN
CCCCCCCCCCCCCCC		66.5125381059
94UbiquitinationGIGNDNSKASERKAS
CCCCCCCHHHHHHHH		63.2923749301
96PhosphorylationGNDNSKASERKASQS
CCCCCHHHHHHHHCC		41.4919823750
101PhosphorylationKASERKASQSSTETQ
HHHHHHHHCCCCCCC		33.3619823750
103PhosphorylationSERKASQSSTETQGP
HHHHHHCCCCCCCCC		36.4120377248
104PhosphorylationERKASQSSTETQGPS
HHHHHCCCCCCCCCC		23.7219823750
105PhosphorylationRKASQSSTETQGPSS
HHHHCCCCCCCCCCC		48.5819823750
107PhosphorylationASQSSTETQGPSSES
HHCCCCCCCCCCCCC		38.6223749301
112PhosphorylationTETQGPSSESGLMTV
CCCCCCCCCCCEEEE		38.6319779198
120UbiquitinationESGLMTVKVYSGKDF
CCCEEEEEEEECCCE		26.7017644757
122PhosphorylationGLMTVKVYSGKDFTL
CEEEEEEEECCCEEE		13.1427017623
123PhosphorylationLMTVKVYSGKDFTLP
EEEEEEEECCCEEEC		42.2127017623
125UbiquitinationTVKVYSGKDFTLPFP
EEEEEECCCEEECCC		43.6717644757
128PhosphorylationVYSGKDFTLPFPITS
EEECCCEEECCCCCC		43.5927017623
142UbiquitinationSNSTILQKLLSSGIL
CCCHHHHHHHHCCCC		48.2017644757
241PhosphorylationKISTFDVTRKLRFLK
CCCCCHHHHHCCCCC		24.7521440633
282UbiquitinationVLKEILKKINTNQDI
HHHHHHHHHCCCCCC		37.4317644757
301UbiquitinationFHLPLNLKIDSAAQI
ECCCCCEEECCCHHH		43.3117644757
335UbiquitinationTVDYKPSKNKPLSID
EEEECCCCCCCCCCC		77.0017644757
337UbiquitinationDYKPSKNKPLSIDDF
EECCCCCCCCCCCCC		51.5817644757
340PhosphorylationPSKNKPLSIDDFDLL
CCCCCCCCCCCCCCC		32.2827017623
348UbiquitinationIDDFDLLKVIGKGSF
CCCCCCCHHHCCCCC		38.8217644757
373AcetylationTQKIYALKALRKAYI
HHHHHHHHHHHHHHE		36.8824489116
499PhosphorylationNMKDNDKTDTFCGTP
CCCCCCCCCCCCCCH		43.0421440633
501PhosphorylationKDNDKTDTFCGTPEY
CCCCCCCCCCCCHHH		26.5125533186
505PhosphorylationKTDTFCGTPEYLAPE
CCCCCCCCHHHHCHH		17.7117330950
508PhosphorylationTFCGTPEYLAPEILL
CCCCCHHHHCHHHHC		14.9030377154
519PhosphorylationEILLGQGYTKTVDWW
HHHCCCCCCCCHHHH		9.3626447709
520PhosphorylationILLGQGYTKTVDWWT
HHCCCCCCCCHHHHH		27.3626447709
522PhosphorylationLGQGYTKTVDWWTLG
CCCCCCCCHHHHHHH		18.8530377154
527PhosphorylationTKTVDWWTLGILLYE
CCCHHHHHHHHHHHH		15.7330377154
533PhosphorylationWTLGILLYEMMTGLP
HHHHHHHHHHHHCCC		9.8030377154
537PhosphorylationILLYEMMTGLPPYYD
HHHHHHHHCCCCCCC		33.9730377154
542PhosphorylationMMTGLPPYYDENVPV
HHHCCCCCCCCCCCH		22.9330377154
543PhosphorylationMTGLPPYYDENVPVM
HHCCCCCCCCCCCHH		23.6630377154
551PhosphorylationDENVPVMYKKILQQP
CCCCCHHHHHHHCCC		14.9030377154
552UbiquitinationENVPVMYKKILQQPL
CCCCHHHHHHHCCCC		18.4217644757
553UbiquitinationNVPVMYKKILQQPLL
CCCHHHHHHHCCCCC		31.0317644757
570UbiquitinationDGFDPAAKDLLIGLL
CCCCHHHHHHHHHHH		51.5217644757
621UbiquitinationPPYKPIVKSEIDTAN
CCCCCCCCCCCCCCC		42.9917644757
622PhosphorylationPYKPIVKSEIDTANF
CCCCCCCCCCCCCCC		28.9627017623
635UbiquitinationNFDQEFTKEKPIDSV
CCCHHHHCCCCCHHH		69.8917644757
641PhosphorylationTKEKPIDSVVDEYLS
HCCCCCHHHHHHHHH		25.4317330950
648PhosphorylationSVVDEYLSASIQKQF
HHHHHHHHHHHHHHH		21.0428889911
650PhosphorylationVDEYLSASIQKQFGG
HHHHHHHHHHHHHCC		23.3717330950
659PhosphorylationQKQFGGWTYIGDEQL
HHHHCCEEEECCCCC		14.4528889911
669PhosphorylationGDEQLGDSPSQGRSI
CCCCCCCCCCCCCCC		25.3428152593
671PhosphorylationEQLGDSPSQGRSIS-
CCCCCCCCCCCCCC-		49.5830377154
Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)
Modified Location Modified Residue Modification Type of Upstream Proteins Gene Name of Upstream Proteins UniProt AC of Upstream Proteins Sources
501TPhosphorylationKinasePKH2Q12236
Uniprot
641SPhosphorylationKinaseTOR2P32600
Uniprot
659TPhosphorylationKinaseTOR2P32600
Uniprot
Functions of PTM Sites
Modified Location Modified Residue Modification Function Reference

Oops, there are no descriptions of PTM sites of YPK2_YEAST !!
Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location Modification Variant Position
(Distance <= 10) 		Residue Change SAP Related Disease Reference

Oops, there are no SNP-PTM records of YPK2_YEAST !!
Protein-Protein Interaction
Interacting Protein Gene Name Interaction Type PPI Reference Domain-Domain Interactions
VATA_YEASTVMA1physical
11805837
YG1A_YEASTYGR016Wphysical
11805837
IF4E_YEASTCDC33physical
11805837
SNF1_YEASTSNF1physical
11805837
YEC3_YEASTYEL023Cphysical
11805837
PRTB_YEASTPRB1physical
11805837
GATB_YEASTPET112physical
11805837
MYO5_YEASTMYO5genetic
16478726
MYO5_YEASTMYO5physical
16478726
ARGR2_YEASTARG81physical
18719252
AVO2_YEASTAVO2genetic
19269370
FPK1_YEASTFPK1genetic
19966303
KIN82_YEASTKIN82genetic
19966303
ATG19_YEASTATG19physical
20489023
FPS1_YEASTFPS1physical
20489023
AMPL_YEASTAPE1physical
20489023
TUS1_YEASTTUS1genetic
11839800
ROM2_YEASTROM2genetic
11839800
RHO2_YEASTRHO2genetic
11839800
KPC1_YEASTPKC1genetic
11839800
BCK1_YEASTBCK1genetic
11839800
MKK1_YEASTMKK1genetic
11839800
BUB1_YEASTBUB1genetic
21127252
GZF3_YEASTGZF3genetic
21127252
KCS1_YEASTKCS1genetic
21127252
RCO1_YEASTRCO1genetic
21127252
SLT2_YEASTSLT2genetic
21127252
MET18_YEASTMET18genetic
21127252
SNF1_YEASTSNF1genetic
21127252
TOR2_YEASTTOR2physical
22207764
AVO1_YEASTAVO1physical
22207764
AVO2_YEASTAVO2physical
22207764
TSC11_YEASTTSC11physical
22207764
LST8_YEASTLST8physical
22207764
BIT61_YEASTBIT61physical
22207764
SSK2_YEASTSSK2genetic
22282571
SWE1_YEASTSWE1genetic
26634277
RPN5_YEASTRPN5genetic
27708008
PSB6_YEASTPRE7genetic
27708008
CDC1_YEASTCDC1genetic
27708008
GPI11_YEASTGPI11genetic
27708008
GPI19_YEASTGPI19genetic
27708008
PSB3_YEASTPUP3genetic
27708008
DPOD2_YEASTPOL31genetic
27708008
PRP19_YEASTPRP19genetic
27708008
NSE1_YEASTNSE1genetic
27708008
NEP1_YEASTEMG1genetic
27708008
DBP6_YEASTDBP6genetic
27708008
HRP1_YEASTHRP1genetic
27708008
TBF1_YEASTTBF1genetic
27708008
HAP3_YEASTHAP3genetic
27708008
RL19A_YEASTRPL19Bgenetic
27708008
RL19B_YEASTRPL19Bgenetic
27708008
VHC1_YEASTVHC1genetic
27708008
SDHF4_YEASTSDH8genetic
27708008
RIM1_YEASTRIM1genetic
27708008
SLX5_YEASTSLX5genetic
27708008
RM01_YEASTMRPL1genetic
27708008
SNA3_YEASTSNA3genetic
27708008
SIW14_YEASTSIW14genetic
27708008
RHO1_YEASTRHO1genetic
28167678
LEM3_YEASTLEM3genetic
28167678
RS5_YEASTRPS5physical
29352143
PLK4_HUMANPLK4physical
27107014
GCP4_HUMANTUBGCP4physical
27107014
CCD33_HUMANCCDC33physical
27107014
LZTS2_HUMANLZTS2physical
27107014
Drug and Disease Associations
Kegg Drug
DrugBank
There are no disease associations of PTM sites.
Regulatory Network of YPK2_YEAST

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Related Literatures of Post-Translational Modification
Phosphorylation
ReferencePubMed
"A multidimensional chromatography technology for in-depthphosphoproteome analysis.";
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
Mol. Cell. Proteomics 7:1389-1396(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-63; SER-72 AND THR-501,AND MASS SPECTROMETRY.
"Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae.";
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.;
J. Proteome Res. 6:1190-1197(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-641 AND SER-650, ANDMASS SPECTROMETRY.
"Tor2 directly phosphorylates the AGC kinase Ypk2 to regulate actinpolarization.";
Kamada Y., Fujioka Y., Suzuki N.N., Inagaki F., Wullschleger S.,Loewith R., Hall M.N., Ohsumi Y.;
Mol. Cell. Biol. 25:7239-7248(2005).
Cited for: FUNCTION, PHOSPHORYLATION AT THR-501 BY PKH2, PHOSPHORYLATION ATSER-641 AND THR-659 BY TOR2, REGULATION BY TOR2, AND MUTAGENESIS OFASP-239; SER-641 AND THR-659.
"Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases.";
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-501, AND MASSSPECTROMETRY.

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