GPD2_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GPD2_YEAST
• UniProt AC: P41911
• Protein Name: Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, mitochondrial
• Gene Name: GPD2
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 440
• Subcellular Localization: Cytoplasm. Mitochondrion.
• Protein Description: Catalyzes the production of glycerol under anaerobic growth conditions. Glycerol production serves as a redox sink by consuming the excess cytosolic NADH during anaerobic metabolism..
• Protein Sequence: MLAVRRLTRYTFLKRTHPVLYTRRAYKILPSRSTFLRRSLLQTQLHSKMTAHTNIKQHKHCHEDHPIRRSDSAVSIVHLKRAPFKVTVIGSGNWGTTIAKVIAENTELHSHIFEPEVRMWVFDEKIGDENLTDIINTRHQNVKYLPNIDLPHNLVADPDLLHSIKGADILVFNIPHQFLPNIVKQLQGHVAPHVRAISCLKGFELGSKGVQLLSSYVTDELGIQCGALSGANLAPEVAKEHWSETTVAYQLPKDYQGDGKDVDHKILKLLFHRPYFHVNVIDDVAGISIAGALKNVVALACGFVEGMGWGNNASAAIQRLGLGEIIKFGRMFFPESKVETYYQESAGVADLITTCSGGRNVKVATYMAKTGKSALEAEKELLNGQSAQGIITCREVHEWLQTCELTQEFPLFEAVYQIVYNNVRMEDLPEMIEELDIDDE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
70	Phosphorylation	EDHPIRRSDSAVSIV CCCCCCCCCCCEEEE	26.52	22369663
72	Phosphorylation	HPIRRSDSAVSIVHL CCCCCCCCCEEEEEE	31.64	22369663
75	Phosphorylation	RRSDSAVSIVHLKRA CCCCCCEEEEEECCC	20.71	22369663
80	Acetylation	AVSIVHLKRAPFKVT CEEEEEECCCCEEEE	31.47	24489116
80	Ubiquitination	AVSIVHLKRAPFKVT CEEEEEECCCCEEEE	31.47	17644757
85	Ubiquitination	HLKRAPFKVTVIGSG EECCCCEEEEEEECC	35.62	17644757
100	Ubiquitination	NWGTTIAKVIAENTE CHHHHHHHHHHHCCC	30.26	17644757
125	Ubiquitination	RMWVFDEKIGDENLT EEEEEECCCCCCCHH	54.56	17644757
143	Ubiquitination	NTRHQNVKYLPNIDL HCCCCCCCCCCCCCC	48.93	17644757
165	Ubiquitination	PDLLHSIKGADILVF HHHHHHCCCCCEEEE	51.57	17644757
184	Ubiquitination	QFLPNIVKQLQGHVA HHHHHHHHHHCCCCC	41.30	17644757
201	Acetylation	VRAISCLKGFELGSK HHHHHHHCCCCCCHH	66.92	24489116
207	Phosphorylation	LKGFELGSKGVQLLS HCCCCCCHHHHHHHH	38.57	27017623
243	Phosphorylation	EVAKEHWSETTVAYQ HHHHHHHCCEEEEEE	27.29	19684113
253	Acetylation	TVAYQLPKDYQGDGK EEEEECCCCCCCCCC	77.11	24489116
260	Acetylation	KDYQGDGKDVDHKIL CCCCCCCCCCCHHHH	60.36	24489116
265	Acetylation	DGKDVDHKILKLLFH CCCCCCHHHHHHHHC	45.32	24489116
268	Acetylation	DVDHKILKLLFHRPY CCCHHHHHHHHCCCC	46.76	24489116
327	Ubiquitination	LGLGEIIKFGRMFFP HCHHHHHHHCCCCCC	47.64	17644757
327	Acetylation	LGLGEIIKFGRMFFP HCHHHHHHHCCCCCC	47.64	24489116
337	Ubiquitination	RMFFPESKVETYYQE CCCCCHHHHHHHHHC	42.29	17644757
369	Ubiquitination	KVATYMAKTGKSALE EEEEEEHHHCHHHHH	41.08	23749301
372	Ubiquitination	TYMAKTGKSALEAEK EEEHHHCHHHHHHHH	37.47	22817900
379	Ubiquitination	KSALEAEKELLNGQS HHHHHHHHHHHCCCC	61.79	15699485
379	Acetylation	KSALEAEKELLNGQS HHHHHHHHHHHCCCC	61.79	24489116

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources