SKP1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SKP1_MOUSE
• UniProt AC: Q9WTX5
• Protein Name: S-phase kinase-associated protein 1
• Gene Name: Skp1
• Organism: Mus musculus (Mouse).
• Sequence Length: 163
• Protein Description: Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2 (By similarity)..
• Protein Sequence: MPTIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQWCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
56	Ubiquitination	NVNAAILKKVIQWCT CHHHHHHHHHHHHHH	38.59	-
78	Ubiquitination	PPEDDENKEKRTDDI CCCCCCCCCCCCCCC	63.67	-
80	Ubiquitination	EDDENKEKRTDDIPV CCCCCCCCCCCCCCC	63.31	-
94	Ubiquitination	VWDQEFLKVDQGTLF CCCHHHHCCCHHHHH	49.43	-
120	S-nitrosocysteine	KGLLDVTCKTVANMI HHHHHHHHHHHHHHH	3.32	-
120	S-nitrosylation	KGLLDVTCKTVANMI HHHHHHHHHHHHHHH	3.32	22178444
128	Ubiquitination	KTVANMIKGKTPEEI HHHHHHHCCCCHHHH	43.52	-
131	Phosphorylation	ANMIKGKTPEEIRKT HHHHCCCCHHHHHHH	44.60	25521595
137	Ubiquitination	KTPEEIRKTFNIKND CCHHHHHHHHCCCCC	64.23	-
142	Ubiquitination	IRKTFNIKNDFTEEE HHHHHCCCCCCCHHH	52.12	-
146	Phosphorylation	FNIKNDFTEEEEAQV HCCCCCCCHHHHHHH	45.40	26525534
155	Ubiquitination	EEEAQVRKENQWCEE HHHHHHHHHHHHHHC	63.26	-
160	S-nitrosocysteine	VRKENQWCEEK---- HHHHHHHHHCC----	3.31	-
160	Glutathionylation	VRKENQWCEEK---- HHHHHHHHHCC----	3.31	24333276
160	S-nitrosylation	VRKENQWCEEK---- HHHHHHHHHCC----	3.31	22178444

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of SKP1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SKP1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SKP1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FBW1B_MOUSE	Fbxw11	physical	11896578
FBW1A_MOUSE	Btrc	physical	10097128
CUL1_MOUSE	Cul1	physical	17242400
MYOG_MOUSE	Myog	physical	17242400
UB2G2_MOUSE	Ube2g2	physical	22306998
UB2D1_HUMAN	UBE2D1	physical	22306998
CKS1_HUMAN	CKS1B	physical	26496610
EPHB4_HUMAN	EPHB4	physical	26496610
FACD2_HUMAN	FANCD2	physical	26496610
PSA1_HUMAN	PSMA1	physical	26496610
PSA3_HUMAN	PSMA3	physical	26496610
PSA6_HUMAN	PSMA6	physical	26496610
PSB2_HUMAN	PSMB2	physical	26496610
PSB6_HUMAN	PSMB6	physical	26496610
SKP2_HUMAN	SKP2	physical	26496610
XPO1_HUMAN	XPO1	physical	26496610
CUL1_HUMAN	CUL1	physical	26496610
RAE1L_HUMAN	RAE1	physical	26496610
FBW1A_HUMAN	BTRC	physical	26496610
KDM2A_HUMAN	KDM2A	physical	26496610
FBX21_HUMAN	FBXO21	physical	26496610
MYCB2_HUMAN	MYCBP2	physical	26496610
FBW1B_HUMAN	FBXW11	physical	26496610
FBX46_HUMAN	FBXO46	physical	26496610
FBX7_HUMAN	FBXO7	physical	26496610
FBX9_HUMAN	FBXO9	physical	26496610
GAR1_HUMAN	GAR1	physical	26496610
FBXW5_HUMAN	FBXW5	physical	26496610
ABCF3_HUMAN	ABCF3	physical	26496610
UTP6_HUMAN	UTP6	physical	26496610
MTUS1_HUMAN	MTUS1	physical	26496610
RT09_HUMAN	MRPS9	physical	26496610
FXL15_HUMAN	FBXL15	physical	26496610
FXL18_HUMAN	FBXL18	physical	26496610
ACTL8_HUMAN	ACTL8	physical	26496610
FBX30_HUMAN	FBXO30	physical	26496610
FBXW9_HUMAN	FBXW9	physical	26496610
KDM2B_HUMAN	KDM2B	physical	26496610
SPRY3_HUMAN	SPRYD3	physical	26496610
TANC1_HUMAN	TANC1	physical	26496610
K2013_HUMAN	KIAA2013	physical	26496610
FXL16_HUMAN	FBXL16	physical	26496610
FBSP1_HUMAN	FBXO45	physical	26496610
CP095_HUMAN	C16orf95	physical	26496610

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.