MYOG_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MYOG_MOUSE
• UniProt AC: P12979
• Protein Name: Myogenin
• Gene Name: Myog
• Organism: Mus musculus (Mouse).
• Sequence Length: 224
• Subcellular Localization: Nucleus . Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos.
• Protein Description: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts..
• Protein Sequence: MELYETSPYFYQEPHFYDGENYLPVHLQGFEPPGYERTELSLSPEARGPLEEKGLGTPEHCPGQCLPWACKVCKRKSVSVDRRRAATLREKRRLKKVNEAFEALKRSTLLNPNQRLPKVEILRSAIQYIERLQALLSSLNQEERDLRYRGGGGPQPMVPSECNSHSASCSPEWGNALEFGPNPGDHLLAADPTDAHNLHSLTSIVDSITVEDMSVAFPDETMPN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
61	S-nitrosocysteine	GLGTPEHCPGQCLPW CCCCCCCCCCCHHHH	3.74	-
61	S-nitrosylation	GLGTPEHCPGQCLPW CCCCCCCCCCCHHHH	3.74	22178444
65	S-nitrosocysteine	PEHCPGQCLPWACKV CCCCCCCHHHHHHHH	6.46	-
65	S-nitrosylation	PEHCPGQCLPWACKV CCCCCCCHHHHHHHH	6.46	22178444
70	S-nitrosocysteine	GQCLPWACKVCKRKS CCHHHHHHHHHCCCC	2.76	-
70	S-nitrosylation	GQCLPWACKVCKRKS CCHHHHHHHHHCCCC	2.76	22178444
77	Phosphorylation	CKVCKRKSVSVDRRR HHHHCCCCCCCCHHH	24.53	-
79	Phosphorylation	VCKRKSVSVDRRRAA HHCCCCCCCCHHHHH	25.95	-
87	Phosphorylation	VDRRRAATLREKRRL CCHHHHHHHHHHHHH	25.93	14751541

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
77	S	Phosphorylation	Kinase	CAMK2G	Q923T9	Uniprot
79	S	Phosphorylation	Kinase	CAMK2G	Q923T9	Uniprot
87	T	Phosphorylation	Kinase	KCC2A	P11798	PhosphoELM
87	T	Phosphorylation	Kinase	CAMK2A	P11275	PSP
87	T	Phosphorylation	Kinase	CAMK2G	Q923T9	Uniprot
87	T	Phosphorylation	Kinase	PRKCA	P17252	GPS
87	T	Phosphorylation	Kinase	PKCA	P05696	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	Fbxo32	Q9CPU7	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
87	T	Phosphorylation	Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MYOG_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EGLN3_HUMAN	EGLN3	physical	17344222
EGLN3_MOUSE	Egln3	physical	17344222
VHL_HUMAN	VHL	physical	17344222
SKP1_MOUSE	Skp1a	physical	17242400
CUL1_MOUSE	Cul1	physical	17242400
CUL2_MOUSE	Cul2	physical	17242400
FBX32_MOUSE	Fbxo32	physical	19631210

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.