dbPTM

BECN1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	BECN1_HUMAN
UniProt AC	Q14457
Protein Name	Beclin-1
Gene Name	BECN1
Organism	Homo sapiens (Human).
Sequence Length	450
Subcellular Localization	Cytoplasm . Golgi apparatus, trans-Golgi network membrane Peripheral membrane protein . Endosome membrane Peripheral membrane protein . Endoplasmic reticulum membrane Peripheral membrane protein . Mitochondrion membrane Peripheral membrane protein
Protein Description	Plays a central role in autophagy. [PubMed: 23184933]
Protein Sequence	MEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQFYNK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEGSKTSN -------CCCCCCCC	13.69	22814378
6	Phosphorylation	--MEGSKTSNNSTMQ --CCCCCCCCCCCCE	38.08	28985074
7	Phosphorylation	-MEGSKTSNNSTMQV -CCCCCCCCCCCCEE	37.44	27251275
10	Phosphorylation	GSKTSNNSTMQVSFV CCCCCCCCCCEEEEE	29.52	27251275
11	Phosphorylation	SKTSNNSTMQVSFVC CCCCCCCCCEEEEEE	17.47	27251275
15	Phosphorylation	NNSTMQVSFVCQRCS CCCCCEEEEEECCCC	8.62	27251275
26	Ubiquitination	QRCSQPLKLDTSFKI CCCCCCCCCCCCEEE	51.50	-
29	Phosphorylation	SQPLKLDTSFKILDR CCCCCCCCCEEEECE	46.64	23403867
30	Phosphorylation	QPLKLDTSFKILDRV CCCCCCCCEEEECEE	24.98	23403867
32	Ubiquitination	LKLDTSFKILDRVTI CCCCCCEEEECEEEH	41.97	21890473
32	Ubiquitination	LKLDTSFKILDRVTI CCCCCCEEEECEEEH	41.97	21890473
38	Phosphorylation	FKILDRVTIQELTAP EEEECEEEHHHHCCC	20.17	20068231
43	Phosphorylation	RVTIQELTAPLLTTA EEEHHHHCCCHHHCC	26.02	26657352
48	Phosphorylation	ELTAPLLTTAQAKPG HHCCCHHHCCCCCCC	27.98	28122231
49	Phosphorylation	LTAPLLTTAQAKPGE HCCCHHHCCCCCCCC	19.42	28122231
53	Ubiquitination	LLTTAQAKPGETQEE HHHCCCCCCCCCCCC	41.28	-
57	Phosphorylation	AQAKPGETQEEETNS CCCCCCCCCCCCCCC	47.39	19369195
62	Phosphorylation	GETQEEETNSGEEPF CCCCCCCCCCCCCCC	37.65	26657352
64	Phosphorylation	TQEEETNSGEEPFIE CCCCCCCCCCCCCCC	55.81	29255136
72	Phosphorylation	GEEPFIETPRQDGVS CCCCCCCCCCCCCCC	20.96	28122231
90	Phosphorylation	IPPARMMSTESANSF CCCHHHCCCCCCCCE	21.49	23878393
91	Phosphorylation	PPARMMSTESANSFT CCHHHCCCCCCCCEE	18.96	30624053
93	Phosphorylation	ARMMSTESANSFTLI HHHCCCCCCCCEEEE	32.60	23878393
96	Phosphorylation	MSTESANSFTLIGEA CCCCCCCCEEEEEEC	21.23	30624053
98	Phosphorylation	TESANSFTLIGEASD CCCCCCEEEEEECCC	19.79	30624053
104	Phosphorylation	FTLIGEASDGGTMEN EEEEEECCCCCCHHH	32.27	22210691
108	Phosphorylation	GEASDGGTMENLSRR EECCCCCCHHHHHHH	27.17	-
113	Phosphorylation	GGTMENLSRRLKVTG CCCHHHHHHHHHHCC	27.26	22210691
117	Ubiquitination	ENLSRRLKVTGDLFD HHHHHHHHHCCCHHH	35.62	20501938
119	Phosphorylation	LSRRLKVTGDLFDIM HHHHHHHCCCHHHHH	24.32	17446862
203	Ubiquitination	QELEDVEKNRKIVAE HHHHHHHHHHHHHHH	63.00	-
206	Acetylation	EDVEKNRKIVAENLE HHHHHHHHHHHHHHH	51.42	20167786
206	Ubiquitination	EDVEKNRKIVAENLE HHHHHHHHHHHHHHH	51.42	-
214	Ubiquitination	IVAENLEKVQAEAER HHHHHHHHHHHHHHH	42.70	-
229	Phosphorylation	LDQEEAQYQREYSEF CHHHHHHHHHHHHHH	19.31	27642862
233	Phosphorylation	EAQYQREYSEFKRQQ HHHHHHHHHHHHHHH	18.71	-
234	Phosphorylation	AQYQREYSEFKRQQL HHHHHHHHHHHHHHC	31.63	-
237	Ubiquitination	QREYSEFKRQQLELD HHHHHHHHHHHCCCH	45.29	-
248	Ubiquitination	LELDDELKSVENQMR CCCHHHHHHHHHHHH	50.86	-
263	Ubiquitination	YAQTQLDKLKKTNVF HHHHHHHHHHHCCCC	71.45	-
266	Ubiquitination	TQLDKLKKTNVFNAT HHHHHHHHCCCCEEE	56.63	-
279	Phosphorylation	ATFHIWHSGQFGTIN EEEEEEECCCCCEEC	21.20	-
295	Phosphorylation	FRLGRLPSVPVEWNE EECCCCCCCCCCHHH	43.58	-
324	Ubiquitination	LANKMGLKFQRYRLV HHHHHCCCEEEEEEE	33.45	-
337	Phosphorylation	LVPYGNHSYLESLTD EECCCCCHHHHHHCC	35.10	-
352	Phosphorylation	KSKELPLYCSGGLRF CCCCCCEEECCCCEE	5.22	-
388	Phosphorylation	EEVEKGETRFCLPYR HHHHCCCCEEEEEEE	37.40	-
402	Ubiquitination	RMDVEKGKIEDTGGS EEEEECCEEEECCCC	54.73	21890473
406	Phosphorylation	EKGKIEDTGGSGGSY ECCEEEECCCCCCCE	30.56	30619164
409	Phosphorylation	KIEDTGGSGGSYSIK EEEECCCCCCCEEEE	41.13	25159151
412	Phosphorylation	DTGGSGGSYSIKTQF ECCCCCCCEEEEEEC	21.16	26699800
413	Phosphorylation	TGGSGGSYSIKTQFN CCCCCCCEEEEEECC	20.07	26699800
414	Phosphorylation	GGSGGSYSIKTQFNS CCCCCCEEEEEECCC	21.21	26699800
417	Phosphorylation	GGSYSIKTQFNSEEQ CCCEEEEEECCCHHH	36.40	30619164
421	Phosphorylation	SIKTQFNSEEQWTKA EEEEECCCHHHHHHH	43.74	24850871
426	Phosphorylation	FNSEEQWTKALKFML CCCHHHHHHHHHHHH	13.21	30619164
427	Ubiquitination	NSEEQWTKALKFMLT CCHHHHHHHHHHHHH	49.08	-
430	Acetylation	EQWTKALKFMLTNLK HHHHHHHHHHHHHHH	33.32	126474619
430	Ubiquitination	EQWTKALKFMLTNLK HHHHHHHHHHHHHHH	33.32	-
437	Acetylation	KFMLTNLKWGLAWVS HHHHHHHHHHHHHHH	41.09	126474625
437	Ubiquitination	KFMLTNLKWGLAWVS HHHHHHHHHHHHHHH	41.09	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
15	S	Phosphorylation	Kinase	ULK1	O75385	PSP
30	S	Phosphorylation	Kinase	ULK1	O75385	PSP
57	T	Phosphorylation	Kinase	ATM	Q13315	PSP
90	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
90	S	Phosphorylation	Kinase	MAPKAPK2	P49137	PSP
90	S	Phosphorylation	Kinase	AMPK	Q9Y478	Uniprot
90	S	Phosphorylation	Kinase	MAPKAPK3	Q16644	PSP
90	S	Phosphorylation	Kinase	DAPK3	O43293	PSP
93	S	Phosphorylation	Kinase	AMPK	Q9Y478	Uniprot
93	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
93	S	Phosphorylation	Kinase	AMPKA1	Q13131	PSP
96	S	Phosphorylation	Kinase	ULK1	O75385	PSP
96	S	Phosphorylation	Kinase	AMPKA1	Q13131	PSP
96	S	Phosphorylation	Kinase	AMPK	Q9Y478	Uniprot
96	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
108	T	Phosphorylation	Kinase	STK4	Q13043	GPS
119	T	Phosphorylation	Kinase	DAPK1	P53355	Uniprot
229	Y	Phosphorylation	Kinase	EGFR	P00533	PSP
233	Y	Phosphorylation	Kinase	PTK2	Q05397	GPS
233	Y	Phosphorylation	Kinase	EGFR	P00533	PSP
234	S	Phosphorylation	Kinase	AKT1	P31749	PSP
279	S	Phosphorylation	Kinase	ULK1	O75385	PSP
295	S	Phosphorylation	Kinase	AKT1	P31749	PSP
337	S	Phosphorylation	Kinase	ULK1	O75385	PSP
352	Y	Phosphorylation	Kinase	EGFR	P00533	PSP
388	T	Phosphorylation	Kinase	PRKAA1	Q13131	GPS
406	T	Phosphorylation	Kinase	CSNK1G2	P78368	GPS
409	S	Phosphorylation	Kinase	CSNK1G2	P78368	GPS
-	K	Ubiquitination	E3 ubiquitin ligase	TRAF6	Q9Y4K3	PMID:20501938
-	K	Ubiquitination	E3 ubiquitin ligase	NEDD4	P46934	PMID:21936852
-	K	Ubiquitination	E3 ubiquitin ligase	AMBRA1	Q9C0C7	PMID:23974797
-	K	Ubiquitination	E3 ubiquitin ligase	KLHL20	Q9Y2M5	PMID:26687681

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
11	K	ubiquitylation	24443581
Polyubiquitinated by NEDD4, both with 'Lys-11'- and 'Lys-63'-linkages.
119	T	Phosphorylation	19180116
Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.
402	K	ubiquitylation	28445460
'Lys-48'-linked polyubiquitination of Lys-402 leads to degradation.
402	K	ubiquitylation	28445460
Polyubiquitinated at Lys-402 with 'Lys-48'-linkages.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of BECN1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TSC1_HUMAN	TSC1	physical	20562859
PK3C3_HUMAN	PIK3C3	physical	20562859
PI3R4_HUMAN	PIK3R4	physical	20562859
S27A4_HUMAN	SLC27A4	physical	20562859
UVRAG_HUMAN	UVRAG	physical	20562859
DZIP3_HUMAN	DZIP3	physical	20562859
PTOV1_HUMAN	PTOV1	physical	20562859
TBCD7_HUMAN	TBC1D7	physical	20562859
BAKOR_HUMAN	ATG14	physical	20562859
RUBIC_HUMAN	KIAA0226	physical	20562859
TRABD_HUMAN	TRABD	physical	20562859
NRBF2_HUMAN	NRBF2	physical	20562859
ASPP2_HUMAN	TP53BP2	physical	20562859
AMRA1_HUMAN	AMBRA1	physical	20562859
ERF3A_HUMAN	GSPT1	physical	20562859
AFG32_HUMAN	AFG3L2	physical	20562859
SEH1_HUMAN	SEH1L	physical	20562859
PRKDC_HUMAN	PRKDC	physical	20562859
RUBCL_HUMAN	KIAA0226L	physical	20562859
ZWINT_HUMAN	ZWINT	physical	20562859
SMC1A_HUMAN	SMC1A	physical	20562859
SMC3_HUMAN	SMC3	physical	20562859
TRAF6_HUMAN	TRAF6	physical	20501938
UBC_HUMAN	UBC	physical	20501938
TNAP3_HUMAN	TNFAIP3	physical	20501938
BECN1_HUMAN	BECN1	physical	20501938
NEDD4_HUMAN	NEDD4	physical	21936852
PK3C3_HUMAN	PIK3C3	physical	21936852
ITCH_HUMAN	ITCH	physical	21936852
AMRA1_HUMAN	AMBRA1	physical	21358617
TAB2_HUMAN	TAB2	physical	21976705
B2CL1_HUMAN	BCL2L1	physical	18628207
VMP1_RAT	Vmp1	physical	23316280
BCL2_HUMAN	BCL2	physical	23316280
BCL2_HUMAN	BCL2	physical	21971985
S10A8_HUMAN	S100A8	physical	21971985
MCL1_HUMAN	MCL1	physical	22219388
B2CL1_HUMAN	BCL2L1	physical	22219388
BCL2_HUMAN	BCL2	physical	23479509
PK3C3_HUMAN	PIK3C3	physical	23974797
WASH1_HUMAN	WASH1	physical	23974797
AMRA1_HUMAN	AMBRA1	physical	23974797
UBP33_HUMAN	USP33	physical	24056301
EXOC2_HUMAN	EXOC2	physical	24056301
BCL2_HUMAN	BCL2	physical	23504944
EXOC8_HUMAN	EXOC8	physical	21241894
BCL2_HUMAN	BCL2	physical	20711182
PK3C3_HUMAN	PIK3C3	physical	20711182
PI3R4_HUMAN	PIK3R4	physical	20711182
AMRA1_HUMAN	AMBRA1	physical	20711182
BAKOR_HUMAN	ATG14	physical	20711182
PI3R4_HUMAN	PIK3R4	physical	19270696
RUBIC_HUMAN	KIAA0226	physical	19270696
PK3C3_HUMAN	PIK3C3	physical	19270696
UVRAG_HUMAN	UVRAG	physical	19270696
BAKOR_HUMAN	ATG14	physical	19270696
BECN1_HUMAN	BECN1	physical	19270696
BCL2_HUMAN	BCL2	physical	19347031
BRCA1_HUMAN	BRCA1	physical	24378767
DACT1_HUMAN	DACT1	physical	24980960
PK3C3_HUMAN	PIK3C3	physical	24980960
BAKOR_HUMAN	ATG14	physical	24980960
RUBIC_HUMAN	KIAA0226	physical	24980960
PK3C3_HUMAN	PIK3C3	physical	24980959
WASH1_HUMAN	WASH1	physical	24980959
BECN1_HUMAN	BECN1	physical	18641390
BCL2_HUMAN	BCL2	physical	18641390
B2CL1_HUMAN	BCL2L1	physical	18641390
UVRAG_HUMAN	UVRAG	physical	18641390
UVRAG_HUMAN	UVRAG	physical	18843052
BAKOR_HUMAN	ATG14	physical	18843052
PK3C3_HUMAN	PIK3C3	physical	18843052
B2CL1_HUMAN	BCL2L1	physical	19049976
BAKOR_HUMAN	ATG14	physical	19050071
UVRAG_HUMAN	UVRAG	physical	19050071
PK3C3_HUMAN	PIK3C3	physical	19050071
BCL2_HUMAN	BCL2	physical	19050071
PI3R4_HUMAN	PIK3R4	physical	19050071
PK3CG_HUMAN	PIK3CG	physical	19050071
PRKN_HUMAN	PARK2	physical	24386307
DAPK1_HUMAN	DAPK1	physical	19180116
B2CL1_HUMAN	BCL2L1	physical	19180116
AMRA1_HUMAN	AMBRA1	physical	20921139
DYL1_HUMAN	DYNLL1	physical	20921139
BCL2_HUMAN	BCL2	physical	23168911
AMRA1_HUMAN	AMBRA1	physical	24587252
BCL2_HUMAN	BCL2	physical	16179260
PK3C3_HUMAN	PIK3C3	physical	16179260
B2CL1_HUMAN	BCL2L1	physical	19713971
PK3C3_HUMAN	PIK3C3	physical	19713971
BCL2_HUMAN	BCL2	physical	19959994
BCL2_HUMAN	BCL2	physical	20622903
PK3C3_HUMAN	PIK3C3	physical	23315026
PK3C3_HUMAN	PIK3C3	physical	23878393
PI3R4_HUMAN	PIK3R4	physical	23878393
BAKOR_HUMAN	ATG14	physical	23878393
UVRAG_HUMAN	UVRAG	physical	23878393
DDB1_HUMAN	DDB1	physical	25499913
AMRA1_HUMAN	AMBRA1	physical	25499913
UBP18_HUMAN	USP18	physical	25906440
PK3C3_HUMAN	PIK3C3	physical	25906440
PK3C3_HUMAN	PIK3C3	physical	26496610
TCOF_HUMAN	TCOF1	physical	26496610
UVRAG_HUMAN	UVRAG	physical	26496610
RUBIC_HUMAN	KIAA0226	physical	26496610
TRIB1_HUMAN	TRIB1	physical	26496610
BAKOR_HUMAN	ATG14	physical	26496610
MDN1_HUMAN	MDN1	physical	26496610
NGDN_HUMAN	NGDN	physical	26496610
LSM3_HUMAN	LSM3	physical	26496610
NRBF2_HUMAN	NRBF2	physical	26496610
PI3R4_HUMAN	PIK3R4	physical	26496610
RN216_HUMAN	RNF216	physical	25484083
BIRC5_HUMAN	BIRC5	physical	26148234
ULK1_HUMAN	ULK1	physical	25891078
IRGM_HUMAN	IRGM	physical	25891078
BAKOR_HUMAN	ATG14	physical	25891078
BCL2_HUMAN	BCL2	physical	25891078
RUBIC_HUMAN	KIAA0226	physical	25891078
BCL2_HUMAN	BCL2	physical	25984893
PK3C3_HUMAN	PIK3C3	physical	25825496
PK3C3_HUMAN	PIK3C3	physical	16390869
EFTU_HUMAN	TUFM	physical	26876213
RUBIC_HUMAN	KIAA0226	physical	26876213
P53_HUMAN	TP53	physical	28128446
NEDD4_HUMAN	NEDD4	physical	28128446
TF65_HUMAN	RELA	physical	28128446
BCL2_HUMAN	BCL2	physical	28128446
PK3C3_HUMAN	PIK3C3	physical	28128446
PK3C3_HUMAN	PIK3C3	physical	25693418
BAKOR_HUMAN	ATG14	physical	25693418
TRAF2_HUMAN	TRAF2	physical	28521610
SPHK1_HUMAN	SPHK1	physical	28521610
PK3C3_HUMAN	PIK3C3	physical	28404643
WDR81_HUMAN	WDR81	physical	28404643
WDR91_HUMAN	WDR91	physical	28404643
BAKOR_HUMAN	ATG14	physical	28404643

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of BECN1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29; SER-30; THR-57;THR-62; SER-64; SER-90; SER-93 AND SER-96, AND MASS SPECTROMETRY.	19369195 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-57 AND SER-64, AND MASSSPECTROMETRY.	18691976 [Abstract]
"DAP-kinase-mediated phosphorylation on the BH3 domain of beclin 1promotes dissociation of beclin 1 from Bcl-XL and induction ofautophagy."; Zalckvar E., Berissi H., Mizrachy L., Idelchuk Y., Koren I.,Eisenstein M., Sabanay H., Pinkas-Kramarski R., Kimchi A.; EMBO Rep. 10:285-292(2009). Cited for: PHOSPHORYLATION AT THR-119, AND INTERACTION WITH DAPK1.	19180116 [Abstract]

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