dbPTM

UBP33_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	UBP33_HUMAN
UniProt AC	Q8TEY7
Protein Name	Ubiquitin carboxyl-terminal hydrolase 33
Gene Name	USP33
Organism	Homo sapiens (Human).
Sequence Length	942
Subcellular Localization	Cytoplasm, perinuclear region . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with centrosomes predominantly in S and G2 phases but less in G1 phase (PubMed:23486064). Isoform 3: Golgi apparatus .
Protein Description	Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains..
Protein Sequence	MTGSNSHITILTLKVLPHFESLGKQEKIPNKMSAFRNHCPHLDSVGEITKEDLIQKSLGTCQDCKVQGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTQPSLPHVRQPHQIQENSVQDFKIPSNTTLKTPLVAVFDDLDIEADEEDELRARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPTTLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEVEEDPQTITTEETMEEDKSQSDVDFQSCESCSNSDRAENENGSRCFSEDNNETTMLIQDDENNSEMSKDWQKEKMCNKINKVNSEGEFDKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDLSTPQILPSNEGVNPRLSASPPKSGNLWPGLAPPHKKAQSASPKRKKQHKKYRSVISDIFDGTIISSVQCLTCDRVSVTLETFQDLSLPIPGKEDLAKLHSSSHPTSIVKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQNFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGVPPRKAGYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAEKIEKRRKTELEIFIRLNRAFQKEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGNVMLRQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDILQAEEKIEVETRSL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MTGSNSHIT ------CCCCCCCEE	49.68	28851738
4	Phosphorylation	----MTGSNSHITIL ----CCCCCCCEEEE	26.45	28851738
6	Phosphorylation	--MTGSNSHITILTL --CCCCCCCEEEEEE	20.26	28851738
9	Phosphorylation	TGSNSHITILTLKVL CCCCCCEEEEEEEEH	12.43	28851738
12	Phosphorylation	NSHITILTLKVLPHF CCCEEEEEEEEHHHH	22.23	28851738
19	Ubiquitination	TLKVLPHFESLGKQE EEEEHHHHHHCCCCC	6.87	21963094
31	Acetylation	KQEKIPNKMSAFRNH CCCCCCCHHHHHHHH	28.95	25953088
34	Ubiquitination	KIPNKMSAFRNHCPH CCCCHHHHHHHHCCC	12.15	32015554
50	Ubiquitination	DSVGEITKEDLIQKS CCCCCCCHHHHHHHH	55.69	21963094
65	Ubiquitination	LGTCQDCKVQGPNLW HCCCCCCCCCCCCHH	45.97	32015554
79	Ubiquitination	WACLENRCSYVGCGE HHHHHCCCCCCCCCC	5.33	23000965
95	Ubiquitination	QVDHSTIHSQETKHY CCCCCCCCCCCCCEE	24.42	29967540
120	Ubiquitination	WCYACSKEVFLDRKL HHHHCCCCHHHCCCC	23.39	29967540
126	Ubiquitination	KEVFLDRKLGTQPSL CCHHHCCCCCCCCCC	51.43	29967540
129	Phosphorylation	FLDRKLGTQPSLPHV HHCCCCCCCCCCCCC	47.98	28555341
132	Phosphorylation	RKLGTQPSLPHVRQP CCCCCCCCCCCCCCC	44.72	28348404
151	Ubiquitination	ENSVQDFKIPSNTTL CCCCCCCCCCCCCCC	63.09	29967540
182	Ubiquitination	EEDELRARGLTGLKN CHHHHHHHCCCCCCC	33.93	21963094
186	Ubiquitination	LRARGLTGLKNIGNT HHHHCCCCCCCCCCC	39.43	21963094
188	Ubiquitination	ARGLTGLKNIGNTCY HHCCCCCCCCCCCHH	48.11	22817900
195	Ubiquitination	KNIGNTCYMNAALQA CCCCCCHHHHHHHHH	7.43	33845483
199	Ubiquitination	NTCYMNAALQALSNC CCHHHHHHHHHHHCC	8.66	29967540
204	Ubiquitination	NAALQALSNCPPLTQ HHHHHHHHCCCCCHH	39.13	33845483
227	Ubiquitination	ARTDKKPAICKSYLK CCCCCCHHHHHHHHH	28.79	23000965
227 (in isoform 2)	Ubiquitination	-	28.79	-
230	Ubiquitination	DKKPAICKSYLKLMT CCCHHHHHHHHHHHH	35.70	29967540
237	Phosphorylation	KSYLKLMTELWHKSR HHHHHHHHHHHHHCC	38.06	27762562
242	Acetylation	LMTELWHKSRPGSVV HHHHHHHHCCCCCCC	36.26	7977275
258	Ubiquitination	TTLFQGIKTVNPTFR CCHHCCCEECCCCCC	53.93	23000965
267	Phosphorylation	VNPTFRGYSQQDAQE CCCCCCCCCHHHHHH	10.07	28152594
268	Phosphorylation	NPTFRGYSQQDAQEF CCCCCCCCHHHHHHH	24.96	28152594
312	Phosphorylation	ETMEEDKSQSDVDFQ HHHHHHCCCCCCCHH	47.43	-
314	Phosphorylation	MEEDKSQSDVDFQSC HHHHCCCCCCCHHHH	46.85	30576142
320	Phosphorylation	QSDVDFQSCESCSNS CCCCCHHHHHCCCCC	21.61	30576142
323	Phosphorylation	VDFQSCESCSNSDRA CCHHHHHCCCCCCCC	27.46	26471730
325	Phosphorylation	FQSCESCSNSDRAEN HHHHHCCCCCCCCCC	48.96	28985074
327	Phosphorylation	SCESCSNSDRAENEN HHHCCCCCCCCCCCC	16.51	30576142
330	Ubiquitination	SCSNSDRAENENGSR CCCCCCCCCCCCCCC	29.02	21963094
333	Ubiquitination	NSDRAENENGSRCFS CCCCCCCCCCCCCCC	55.24	22817900
334	Ubiquitination	SDRAENENGSRCFSE CCCCCCCCCCCCCCC	66.38	21963094
336	Ubiquitination	RAENENGSRCFSEDN CCCCCCCCCCCCCCC	37.14	22817900
338	Ubiquitination	ENENGSRCFSEDNNE CCCCCCCCCCCCCCC	4.75	33845483
340	Ubiquitination	ENGSRCFSEDNNETT CCCCCCCCCCCCCCE	47.90	29967540
343	Ubiquitination	SRCFSEDNNETTMLI CCCCCCCCCCCEEEE	44.15	33845483
352	Ubiquitination	ETTMLIQDDENNSEM CCEEEEECCCCCCCC	59.19	33845483
352 (in isoform 2)	Ubiquitination	-	59.19	21906983
361	Ubiquitination	ENNSEMSKDWQKEKM CCCCCCCHHHHHHHH	62.77	21963094
365	Ubiquitination	EMSKDWQKEKMCNKI CCCHHHHHHHHHHHH	55.86	21963094
367	Ubiquitination	SKDWQKEKMCNKINK CHHHHHHHHHHHHHH	56.65	22817900
370	Ubiquitination	WQKEKMCNKINKVNS HHHHHHHHHHHHCCC	44.75	29967540
371	Ubiquitination	QKEKMCNKINKVNSE HHHHHHHHHHHCCCC	42.61	29967540
374	Ubiquitination	KMCNKINKVNSEGEF HHHHHHHHCCCCCCC	46.39	33845483
377	Phosphorylation	NKINKVNSEGEFDKD HHHHHCCCCCCCCCC	50.38	25159151
383	Ubiquitination	NSEGEFDKDRDSISE CCCCCCCCCCCCCCE	61.04	21906983
383 (in isoform 1)	Ubiquitination	-	61.04	21906983
383 (in isoform 3)	Ubiquitination	-	61.04	21906983
387	Phosphorylation	EFDKDRDSISETVDL CCCCCCCCCCEEEEC	28.98	30576142
389	Phosphorylation	DKDRDSISETVDLNN CCCCCCCCEEEECCC	31.03	25159151
391	Phosphorylation	DRDSISETVDLNNQE CCCCCCEEEECCCCC	16.72	27690223
401	Ubiquitination	LNNQETVKVQIHSRA CCCCCEEEEEEEECC	35.52	29967540
404	Ubiquitination	QETVKVQIHSRASEY CCEEEEEEEECCHHH	3.40	21963094
409	Phosphorylation	VQIHSRASEYITDVH EEEEECCHHHEEECC	29.80	28796482
411	Phosphorylation	IHSRASEYITDVHSN EEECCHHHEEECCCC	13.46	28796482
411	Ubiquitination	IHSRASEYITDVHSN EEECCHHHEEECCCC	13.46	29967540
413	Phosphorylation	SRASEYITDVHSNDL ECCHHHEEECCCCCC	30.94	28796482
417	Phosphorylation	EYITDVHSNDLSTPQ HHEEECCCCCCCCCC	31.71	28796482
421	Phosphorylation	DVHSNDLSTPQILPS ECCCCCCCCCCCCCC	40.69	28555341
422	Phosphorylation	VHSNDLSTPQILPSN CCCCCCCCCCCCCCC	26.89	28555341
437	Phosphorylation	EGVNPRLSASPPKSG CCCCCCCCCCCCCCC	28.16	30266825
439	Phosphorylation	VNPRLSASPPKSGNL CCCCCCCCCCCCCCC	38.06	10470851
442	Ubiquitination	RLSASPPKSGNLWPG CCCCCCCCCCCCCCC	74.51	29967540
443	Phosphorylation	LSASPPKSGNLWPGL CCCCCCCCCCCCCCC	38.39	25159151
461	Phosphorylation	HKKAQSASPKRKKQH CCCCCCCCHHHHHHH	35.88	-
481	Ubiquitination	VISDIFDGTIISSVQ HHHHHCCCCCEEEEE	14.20	22817900
481 (in isoform 2)	Ubiquitination	-	14.20	21906983
486	Ubiquitination	FDGTIISSVQCLTCD CCCCCEEEEEEECCC	13.05	33845483
512	Ubiquitination	LSLPIPGKEDLAKLH CCCCCCCHHHHHHHH	42.58	22817900
512 (in isoform 1)	Ubiquitination	-	42.58	21906983
512 (in isoform 3)	Ubiquitination	-	42.58	21906983
517	Ubiquitination	PGKEDLAKLHSSSHP CCHHHHHHHHCCCCC	54.77	33845483
544	Ubiquitination	YAPQGWIAFFMEYVK CCCCCHHHHHHHHHH	5.77	21963094
552	Ubiquitination	FFMEYVKRFVVSCVP HHHHHHHHHHHHHCC	21.39	21963094
575	Ubiquitination	TLQDCLAAFFARDEL EHHHHHHHHHHCHHH	6.27	21963094
583	Ubiquitination	FFARDELKGDNMYSC HHHCHHHCCCCCCCH	63.27	21963094
588	Phosphorylation	ELKGDNMYSCEKCKK HHCCCCCCCHHHHHH	19.20	-
617	Ubiquitination	EILCIHLKRFRHELM CCEEEEHHHHCHHHH	35.02	-
668	Phosphorylation	SVICHHGTASSGHYI HHHCCCCCCCCCCCE	21.00	-
670	Phosphorylation	ICHHGTASSGHYIAY HCCCCCCCCCCCEEE	36.37	-
671	Phosphorylation	CHHGTASSGHYIAYC CCCCCCCCCCCEEEE	27.42	-
684	Ubiquitination	YCRNNLNNLWYEFDD EECCCCCCEEEEECC	34.81	32142685
686	Ubiquitination	RNNLNNLWYEFDDQS CCCCCCEEEEECCCC	7.85	23503661
698	Ubiquitination	DQSVTEVSESTVQNA CCCEEEECHHHHHCE	21.43	22817900
703	Ubiquitination	EVSESTVQNAEAYVL EECHHHHHCEEEEEE	43.43	21963094
714	Acetylation	AYVLFYRKSSEEAQK EEEEEEECCHHHHHH	47.13	155477
725	Ubiquitination	EAQKERRRISNLLNI HHHHHHHHHHHHHHH	42.80	32142685
727	Phosphorylation	QKERRRISNLLNIME HHHHHHHHHHHHHHC	21.44	24719451
727	Ubiquitination	QKERRRISNLLNIME HHHHHHHHHHHHHHC	21.44	23503661
733	Ubiquitination	ISNLLNIMEPSLLQF HHHHHHHHCHHHHHH	6.36	32142685
735	Ubiquitination	NLLNIMEPSLLQFYI HHHHHHCHHHHHHHH	16.94	23503661
736	Phosphorylation	LLNIMEPSLLQFYIS HHHHHCHHHHHHHHH	28.53	24719451
739	Ubiquitination	IMEPSLLQFYISRQW HHCHHHHHHHHHHHH	34.10	22817900
741	Phosphorylation	EPSLLQFYISRQWLN CHHHHHHHHHHHHHH	5.75	-
743	Phosphorylation	SLLQFYISRQWLNKF HHHHHHHHHHHHHHC	13.67	24719451
744	Ubiquitination	LLQFYISRQWLNKFK HHHHHHHHHHHHHCC	23.33	21963094
747	Ubiquitination	FYISRQWLNKFKTFA HHHHHHHHHHCCCCC	3.65	22817900
752	Ubiquitination	QWLNKFKTFAEPGPI HHHHHCCCCCCCCCC	31.07	21963094
764	Ubiquitination	GPISNNDFLCIHGGV CCCCCCCEEEEECCC	6.96	32142685
766	Ubiquitination	ISNNDFLCIHGGVPP CCCCCEEEEECCCCC	1.87	23503661
778	Ubiquitination	VPPRKAGYIEDLVLM CCCCCCCHHHHHHHH	13.14	22817900
783	Ubiquitination	AGYIEDLVLMLPQNI CCHHHHHHHHCCHHH	4.58	21963094
796	Phosphorylation	NIWDNLYSRYGGGPA HHHHHHHHHHCCCCC	23.38	24719451
824	Ubiquitination	AEKIEKRRKTELEIF HHHHHHHHHHHHHHH	62.41	32142685
826	Ubiquitination	KIEKRRKTELEIFIR HHHHHHHHHHHHHHH	43.95	23503661
832	Ubiquitination	KTELEIFIRLNRAFQ HHHHHHHHHHHHHHH	6.34	32142685
832 (in isoform 2)	Ubiquitination	-	6.34	-
834	Ubiquitination	ELEIFIRLNRAFQKE HHHHHHHHHHHHHCC	4.32	23503661
838	Ubiquitination	FIRLNRAFQKEDSPA HHHHHHHHHCCCCCC	10.87	22817900
843	Ubiquitination	RAFQKEDSPATFYCI HHHHCCCCCCEEEEE	19.95	21963094
846	Ubiquitination	QKEDSPATFYCISMQ HCCCCCCEEEEEEHH	20.65	22817900
851	Ubiquitination	PATFYCISMQWFREW CCEEEEEEHHHHHHH	11.02	21963094
855	Ubiquitination	YCISMQWFREWESFV EEEEHHHHHHHHHHH	2.60	32142685
857	Ubiquitination	ISMQWFREWESFVKG EEHHHHHHHHHHHCC	46.23	23503661
863	Ubiquitination	REWESFVKGKDGDPP HHHHHHHCCCCCCCC	59.23	32142685
865	Ubiquitination	WESFVKGKDGDPPGP HHHHHCCCCCCCCCC	53.47	23503661
869	Ubiquitination	VKGKDGDPPGPIDNT HCCCCCCCCCCCCCC	41.08	22817900
874	Ubiquitination	GDPPGPIDNTKIAVT CCCCCCCCCCEEEEE	60.94	21963094
877	Ubiquitination	PGPIDNTKIAVTKCG CCCCCCCEEEEEECC	34.62	22817900
882	Ubiquitination	NTKIAVTKCGNVMLR CCEEEEEECCCEEEE	33.28	21963094
907	Phosphorylation	ETWNFLQSIYGGGPE HHHHHHHHHHCCCCE	21.67	-
909	Phosphorylation	WNFLQSIYGGGPEVI HHHHHHHHCCCCEEE	18.24	-
941	Phosphorylation	KIEVETRSL------ HHEEEECCC------	45.94	29514088

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	VHL	P40337	PMID:11739384

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of UBP33_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of UBP33_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
IOD2_HUMAN	DIO2	physical	12865408
SBP1_HUMAN	SELENBP1	physical	19118533
KRR1_HUMAN	KRR1	physical	19615732
IFIT5_HUMAN	IFIT5	physical	19615732
ZFR_HUMAN	ZFR	physical	19615732
PR38B_HUMAN	PRPF38B	physical	19615732
ARRB2_HUMAN	ARRB2	physical	19363159
ARRB1_HUMAN	ARRB1	physical	19363159
ROBO1_HUMAN	ROBO1	physical	19706539
CP110_HUMAN	CCP110	physical	23486064
UBP20_HUMAN	USP20	physical	23486064
NEUL4_HUMAN	NEURL4	physical	23486064
RALA_HUMAN	RALA	physical	24056301
RALB_HUMAN	RALB	physical	24056301
UBC_HUMAN	UBC	physical	24377933
ARRB2_HUMAN	ARRB2	physical	24377933
TRAF6_HUMAN	TRAF6	physical	21525354
ARRB2_HUMAN	ARRB2	physical	27835898

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of UBP33_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-439, AND MASSSPECTROMETRY.	18669648 [Abstract]