SPHK1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SPHK1_HUMAN
• UniProt AC: Q9NYA1
• Protein Name: Sphingosine kinase 1
• Gene Name: SPHK1
• Organism: Homo sapiens (Human).
• Sequence Length: 384
• Subcellular Localization: Cytoplasm. Nucleus. Cell membrane. Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.
• Protein Description: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol..
• Protein Sequence: MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
36 (in isoform 2)	Phosphorylation	-	22.22	-
46 (in isoform 2)	Phosphorylation	-	30.58	-
55 (in isoform 2)	Phosphorylation	-	45.76	24719451
58 (in isoform 2)	Acetylation	-	39.12	-
193	Phosphorylation	RLGEMRFTLGTFLRL HHHCHHHHHHHHHHH	17.57	18669648
225	Phosphorylation	VGSKTPASPVVVQQG CCCCCCCCCEEEEEC	21.30	15623571
279 (in isoform 2)	Phosphorylation	-	10.72	27251275
295	Phosphorylation	FYVRAGVSRAMLLRL HHHHHCCCHHHHHHH	17.26	24719451
381 (in isoform 2)	Phosphorylation	-	77.38	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
225	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
225	S	Phosphorylation	Kinase	MAPK3	P27361	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SPHK1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SPHK1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TRAF2_HUMAN	TRAF2	physical	11777919
MBTP1_HUMAN	MBTPS1	physical	17124500
BIRC3_HUMAN	BIRC3	physical	24464131
DCXR_HUMAN	DCXR	physical	26186194
PPM1G_HUMAN	PPM1G	physical	26186194
DHYS_HUMAN	DHPS	physical	26186194
CYLD_HUMAN	CYLD	physical	26186194
CYLD_HUMAN	CYLD	physical	28514442
DCXR_HUMAN	DCXR	physical	28514442
DHYS_HUMAN	DHPS	physical	28514442
BECN1_HUMAN	BECN1	physical	28521610
TRAF2_HUMAN	TRAF2	physical	28521610

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• DB08868: Fingolimod

Related Literatures of Post-Translational Modification

Phosphorylation

"Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitinligase TRAF2.";
Alvarez S.E., Harikumar K.B., Hait N.C., Allegood J., Strub G.M.,Kim E.Y., Maceyka M., Jiang H., Luo C., Kordula T., Milstien S.,Spiegel S.;
Nature 465:1084-1088(2010).
Cited for: CATALYTIC ACTIVITY, FUNCTION, MUTAGENESIS OF GLY-82, ANDPHOSPHORYLATION AT SER-225.
PubMed: 20577214

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-193, AND MASSSPECTROMETRY.
PubMed: 18669648