DACT1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DACT1_HUMAN
• UniProt AC: Q9NYF0
• Protein Name: Dapper homolog 1
• Gene Name: DACT1
• Organism: Homo sapiens (Human).
• Sequence Length: 836
• Subcellular Localization: Cytoplasm. Nucleus. Cell junction, synapse. Shuttles between the nucleus and the cytoplasm. Seems to be nuclear in the absence of Wnt signaling and to translocate to the cytoplasm in its presence.
• Protein Description: Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome-dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB. Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway (By similarity). The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling. May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines..
• Protein Sequence: MKPSPAGTAKELEPPAPARGEQRTAEPEGRWREKGEADTERQRTRERQEATLAGLAELEYLRQRQELLVRGALRGAGGAGAAAPRAGELLGEAAQRSRLEEKFLEENILLLRKQLNCLRRRDAGLLNQLQELDKQISDLRLDVEKTSEEHLETDSRPSSGFYELSDGASGSLSNSSNSVFSECLSSCHSSTCFCSPLEATLSLSDGCPKSADLIGLLEYKEGHCEDQASGAVCRSLSTPQFNSLDVIADVNPKYQCDLVSKNGNDVYRYPSPLHAVAVQSPMFLLCLTGNPLREEDRLGNHASDICGGSELDAVKTDSSLPSPSSLWSASHPSSSKKMDGYILSLVQKKTHPVRTNKPRTSVNADPTKGLLRNGSVCVRAPGGVSQGNSVNLKNSKQACLPSGGIPSLNNGTFSPPKQWSKESKAEQAESKRVPLPEGCPSGAASDLQSKHLPKTAKPASQEHARCSAIGTGESPKESAQLSGASPKESPSRGPAPPQENKVVQPLKKMSQKNSLQGVPPATPPLLSTAFPVEERPALDFKSEGSSQSLEEAHLVKAQFIPGQQPSVRLHRGHRNMGVVKNSSLKHRGPALQGLENGLPTVREKTRAGSKKCRFPDDLDTNKKLKKASSKGRKSGGGPEAGVPGRPAGGGHRAGSRAHGHGREAVVAKPKHKRTDYRRWKSSAEISYEEALRRARRGRRENVGLYPAPVPLPYASPYAYVASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSVSEGEFVGESTTTSDSEESGGLIWSQFVQTLPIQTVTAPDLHNHPAKTFVKIKASHNLKKKILRFRSGSLKLMTTV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
51	Phosphorylation	TRERQEATLAGLAEL HHHHHHHHHHHHHHH	19.18	23403867
60	Phosphorylation	AGLAELEYLRQRQEL HHHHHHHHHHHHHHH	21.58	23403867
70	Methylation	QRQELLVRGALRGAG HHHHHHHHHHHCCCC	25.33	-
137	Phosphorylation	QELDKQISDLRLDVE HHHHHHHHHHCCCHH	28.26	24719451
235	Phosphorylation	ASGAVCRSLSTPQFN HCCCHHHCCCCCCCC	22.88	27251275
237	Phosphorylation	GAVCRSLSTPQFNSL CCHHHCCCCCCCCCC	39.01	21262972
238	Phosphorylation	AVCRSLSTPQFNSLD CHHHCCCCCCCCCCE	26.80	27251275
325	Phosphorylation	SSLPSPSSLWSASHP CCCCCHHHHHCCCCC	36.92	-
360	Phosphorylation	VRTNKPRTSVNADPT CCCCCCCCCCCCCCC	45.62	23312004
361	Phosphorylation	RTNKPRTSVNADPTK CCCCCCCCCCCCCCC	18.04	23312004
367	Phosphorylation	TSVNADPTKGLLRNG CCCCCCCCCCCCCCC	37.99	-
420	Phosphorylation	FSPPKQWSKESKAEQ CCCCCCCCHHHHHHH	24.85	-
423	Phosphorylation	PKQWSKESKAEQAES CCCCCHHHHHHHHHH	40.68	-
430	Phosphorylation	SKAEQAESKRVPLPE HHHHHHHHCCCCCCC	28.86	-
478	Phosphorylation	TGESPKESAQLSGAS CCCCHHHHHHHCCCC	27.69	24706070
482	Phosphorylation	PKESAQLSGASPKES HHHHHHHCCCCCCCC	21.89	22985185
485	Phosphorylation	SAQLSGASPKESPSR HHHHCCCCCCCCCCC	39.78	22985185
514	Phosphorylation	KKMSQKNSLQGVPPA HHHHHCCCCCCCCCC	29.16	29255136
522	Phosphorylation	LQGVPPATPPLLSTA CCCCCCCCCCCCCCC	31.08	29255136
527	Phosphorylation	PATPPLLSTAFPVEE CCCCCCCCCCCCCCC	25.93	29255136
528	Phosphorylation	ATPPLLSTAFPVEER CCCCCCCCCCCCCCC	32.08	29255136
545	Phosphorylation	LDFKSEGSSQSLEEA CCCCCCCCCCCHHHH	22.58	23312004
546	Phosphorylation	DFKSEGSSQSLEEAH CCCCCCCCCCHHHHH	34.35	23312004
548	Phosphorylation	KSEGSSQSLEEAHLV CCCCCCCCHHHHHEE	39.43	28857561
566	Phosphorylation	FIPGQQPSVRLHRGH ECCCCCCCEEEECCC	18.61	28857561
582	Phosphorylation	NMGVVKNSSLKHRGP CCCCCCCCCCCCCCH	31.18	-
583	Phosphorylation	MGVVKNSSLKHRGPA CCCCCCCCCCCCCHH	51.32	-
628	Phosphorylation	NKKLKKASSKGRKSG CHHHHHHHHHCCCCC	41.27	29457462
629	Phosphorylation	KKLKKASSKGRKSGG HHHHHHHHHCCCCCC	43.95	29457462
634	Phosphorylation	ASSKGRKSGGGPEAG HHHHCCCCCCCCCCC	41.11	28555341
655	Phosphorylation	GGGHRAGSRAHGHGR CCCCCCCCCCCCCCC	25.88	-
681	Phosphorylation	TDYRRWKSSAEISYE CCHHHHHHHHCCCHH	28.02	27251275
686	Phosphorylation	WKSSAEISYEEALRR HHHHHCCCHHHHHHH	20.28	26330541
687	Phosphorylation	KSSAEISYEEALRRA HHHHCCCHHHHHHHH	24.37	26330541
827	Phosphorylation	KKILRFRSGSLKLMT HHHHHCCCCCCEEEE	29.98	21262972
829	Phosphorylation	ILRFRSGSLKLMTTV HHHCCCCCCEEEEEC	24.47	29514088

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
237	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
237	S	Phosphorylation	Kinase	PKA	-	Uniprot
827	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
827	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of DACT1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DACT1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LEF1_HUMAN	LEF1	physical	18936100
HDAC1_HUMAN	HDAC1	physical	18936100
BECN1_HUMAN	BECN1	physical	24980960
PK3C3_HUMAN	PIK3C3	physical	24980960
BAKOR_HUMAN	ATG14	physical	24980960
ZBT17_HUMAN	ZBTB17	physical	25558878
VHL_HUMAN	VHL	physical	25825496
1433B_HUMAN	YWHAB	physical	21262972
KAPCA_HUMAN	PRKACA	physical	21262972
DVL2_HUMAN	DVL2	physical	21262972

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 182940: Neural tube defects (NTD)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.