WASH1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: WASH1_HUMAN
• UniProt AC: A8K0Z3
• Protein Name: WASH complex subunit 1 {ECO:0000312|HGNC:HGNC:24361}
• Gene Name: WASHC1 {ECO:0000312|HGNC:HGNC:24361}
• Organism: Homo sapiens (Human).
• Sequence Length: 465
• Subcellular Localization: Early endosome membrane . Recycling endosome membrane . Late endosome . Cytoplasmic vesicle, autophagosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Localization to the endosome membrane is mediated via its int
• Protein Description: Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. [PubMed: 19922874]
• Protein Sequence: MTPVRMQHSLAGQTYAVPFIQPDLRREEAVQQMADALQYLQKVSGDIFSRISQQVEQSRSQVQAIGEKVSLAQAKIEKIKGSKKAIKVFSSAKYPAPGRLQEYGSIFTGAQDPGLQRRPRHRIQSKHRPLDERALQEKLKDFPVCVSTKPEPEDDAEEGLGGLPSNISSVSSLLLFNTTENLYKKYVFLDPLAGAVTKTHVMLGAETEEKLFDAPLSISKREQLEQQVPENYFYVPDLGQVPEIHVPSYLPDLPGIANDLMYSADLGPGIAPSAPGTIPELPTFHTEVAEPLKVDLQDGVLTPPPPPPPPPPAPEVLASAPPLPPSTAAPVGQGARQDDSSSSASPSVQGAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKQQQKEQEQVRATSQGGHLMSDLFNKLVMRRKGISGKGPGAGEGPGGAFVRVSDSIPPLPPPQQPQAEEDEDDWES

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
39	Phosphorylation	QMADALQYLQKVSGD HHHHHHHHHHHHCHH	16.38	24114839
52	Phosphorylation	GDIFSRISQQVEQSR HHHHHHHHHHHHHHH	17.28	-
68	Ubiquitination	QVQAIGEKVSLAQAK HHHHHHHHHHHHHHH	31.73	-
75	Ubiquitination	KVSLAQAKIEKIKGS HHHHHHHHHHHHCCC	39.04	29967540
103	Phosphorylation	APGRLQEYGSIFTGA CCCCHHHHCCCCCCC	11.83	29978859
105	Phosphorylation	GRLQEYGSIFTGAQD CCHHHHCCCCCCCCC	17.39	28857561
113	Ubiquitination	IFTGAQDPGLQRRPR CCCCCCCCCCCCCCC	33.06	27667366
114	Ubiquitination	FTGAQDPGLQRRPRH CCCCCCCCCCCCCCH	42.61	27667366
124	Ubiquitination	RRPRHRIQSKHRPLD CCCCHHHHCCCCCCC	46.51	27667366
125	Ubiquitination	RPRHRIQSKHRPLDE CCCHHHHCCCCCCCH	28.23	27667366
137	Ubiquitination	LDERALQEKLKDFPV CCHHHHHHHHCCCCE	62.29	27667366
138	Ubiquitination	DERALQEKLKDFPVC CHHHHHHHHCCCCEE	48.12	27667366
141	Phosphorylation	ALQEKLKDFPVCVST HHHHHHCCCCEEEEC	64.62	-
146	Ubiquitination	LKDFPVCVSTKPEPE HCCCCEEEECCCCCC	9.19	27667366
147	Ubiquitination	KDFPVCVSTKPEPED CCCCEEEECCCCCCC	26.11	27667366
152	Ubiquitination	CVSTKPEPEDDAEEG EEECCCCCCCCHHHC	59.86	27667366
185	Ubiquitination	TTENLYKKYVFLDPL CCCCHHHHHHCCCCC	32.84	-
186	Phosphorylation	TENLYKKYVFLDPLA CCCHHHHHHCCCCCC	7.71	25106551
198	Ubiquitination	PLAGAVTKTHVMLGA CCCCCCCCCEECCCC	29.99	32015554
202	Sulfoxidation	AVTKTHVMLGAETEE CCCCCEECCCCCCHH	1.91	21406390
217	Phosphorylation	KLFDAPLSISKREQL HHHCCCCCCCHHHHH	24.61	25159151
219	Phosphorylation	FDAPLSISKREQLEQ HCCCCCCCHHHHHHH	23.45	25159151
220	Ubiquitination	DAPLSISKREQLEQQ CCCCCCCHHHHHHHH	59.28	32015554
280	Ubiquitination	SAPGTIPELPTFHTE CCCCCCCCCCCCCCC	64.63	24816145
340	Phosphorylation	QGARQDDSSSSASPS CCCCCCCCCCCCCCC	40.17	28634120
341	Phosphorylation	GARQDDSSSSASPSV CCCCCCCCCCCCCCC	34.99	29978859
342	Phosphorylation	ARQDDSSSSASPSVQ CCCCCCCCCCCCCCC	33.60	29978859
343	Phosphorylation	RQDDSSSSASPSVQG CCCCCCCCCCCCCCC	34.41	25849741
345	Phosphorylation	DDSSSSASPSVQGAP CCCCCCCCCCCCCCC	21.47	23401153
347	Phosphorylation	SSSSASPSVQGAPRE CCCCCCCCCCCCCCE	25.28	20068231
352	Ubiquitination	SPSVQGAPREVVDPS CCCCCCCCCEEECCC	39.53	24816145
353	Ubiquitination	PSVQGAPREVVDPSG CCCCCCCCEEECCCC	49.13	24816145
363	Ubiquitination	VDPSGGWATLLESIR ECCCCHHHHHHHHHH	7.52	24816145
364	Ubiquitination	DPSGGWATLLESIRQ CCCCHHHHHHHHHHH	25.93	24816145
368	Phosphorylation	GWATLLESIRQAGGI HHHHHHHHHHHCCCC	24.04	29255136
376	Ubiquitination	IRQAGGIGKAKLRSM HHHCCCCCHHHHHHH	27.43	24816145
377	Ubiquitination	RQAGGIGKAKLRSMK HHCCCCCHHHHHHHH	40.19	24816145
385	Ubiquitination	AKLRSMKERKLEKQQ HHHHHHHHHHHHHHH	46.54	24816145
386	Ubiquitination	KLRSMKERKLEKQQQ HHHHHHHHHHHHHHH	43.26	24816145
391	Ubiquitination	KERKLEKQQQKEQEQ HHHHHHHHHHHHHHH	41.37	24816145
402	Phosphorylation	EQEQVRATSQGGHLM HHHHHHHHHHHHHHH	15.83	-
403	Phosphorylation	QEQVRATSQGGHLMS HHHHHHHHHHHHHHH	26.33	-
410	Phosphorylation	SQGGHLMSDLFNKLV HHHHHHHHHHHHHHH	37.21	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of WASH1_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
220	K	ubiquitylation	Ubiquitinated at Lys-220 via 'Lys-63'-linked ubiquitin chains by the TRIM27:MAGEL2 E3 ubiquitin ligase complex, leading to promote endosomal F-actin assembly.	23452853

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of WASH1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BECN1_HUMAN	BECN1	physical	23974797
BL1S2_HUMAN	BLOC1S2	physical	23974797

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.