CHM4B_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CHM4B_MOUSE
• UniProt AC: Q9D8B3
• Protein Name: Charged multivesicular body protein 4b
• Gene Name: Chmp4b
• Organism: Mus musculus (Mouse).
• Sequence Length: 224
• Subcellular Localization: Cytoplasm, cytosol . Late endosome membrane ; Peripheral membrane protein . Midbody . Nucleus envelope . Recruited to the nuclear envelope by CHMP7 during late anaphase. Localizes transiently to the midbody arms immediately before abscission.
• Protein Description: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Plays a role in the endosomal sorting pathway. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan..
• Protein Sequence: MSVFGKLFGAGGGKAGKGGPTPQEAIQRLRDTEEMLSKKQEFLEKKIEQELTAAKKHGTKNKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGYAAKAMKAAHDNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNLLEISGPETVPLPNVPSVALPSKPAKKKEEEDDDMKELENWAGSM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSVFGKLFG ------CCHHHHCCC	26.59	23806337
2	Phosphorylation	------MSVFGKLFG ------CCHHHHCCC	26.59	29514104
6	Acetylation	--MSVFGKLFGAGGG --CCHHHHCCCCCCC	29.22	23806337
14	Ubiquitination	LFGAGGGKAGKGGPT CCCCCCCCCCCCCCC	58.18	27667366
87	Phosphorylation	AQIDGTLSTIEFQRE HHCCCCHHHHHHHHH	27.46	26525534
88	Phosphorylation	QIDGTLSTIEFQREA HCCCCHHHHHHHHHH	27.86	26525534
107	Ubiquitination	NTNTEVLKNMGYAAK CCCHHHHHHHHHHHH	50.49	22790023
114	Acetylation	KNMGYAAKAMKAAHD HHHHHHHHHHHHHHH	40.62	22826441
184	Phosphorylation	DKNLLEISGPETVPL HHHCHHHHCCCCCCC	38.63	26239621
188	Phosphorylation	LEISGPETVPLPNVP HHHHCCCCCCCCCCC	30.47	23984901
196	Phosphorylation	VPLPNVPSVALPSKP CCCCCCCCCCCCCCC	18.75	29514104
223	Phosphorylation	ELENWAGSM------ HHHHHHHCC------	17.36	25521595

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of CHM4B_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of CHM4B_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CHM4B_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
VATB2_HUMAN	ATP6V1B2	physical	26496610
VATE1_HUMAN	ATP6V1E1	physical	26496610
VAS1_HUMAN	ATP6AP1	physical	26496610
BST2_HUMAN	BST2	physical	26496610
CAV1_HUMAN	CAV1	physical	26496610
CBPM_HUMAN	CPM	physical	26496610
DSG2_HUMAN	DSG2	physical	26496610
STOM_HUMAN	STOM	physical	26496610
FLOT2_HUMAN	FLOT2	physical	26496610
GNAI3_HUMAN	GNAI3	physical	26496610
IPP_HUMAN	IPP	physical	26496610
PLAK_HUMAN	JUP	physical	26496610
KTN1_HUMAN	KTN1	physical	26496610
CHM1A_HUMAN	CHMP1A	physical	26496610
ABCD3_HUMAN	ABCD3	physical	26496610
RBBP6_HUMAN	RBBP6	physical	26496610
SDCB1_HUMAN	SDCBP	physical	26496610
SOAT1_HUMAN	SOAT1	physical	26496610
UAP1_HUMAN	UAP1	physical	26496610
TFR1_HUMAN	TFRC	physical	26496610
NELFA_HUMAN	NELFA	physical	26496610
DX39B_HUMAN	DDX39B	physical	26496610
PABP2_HUMAN	PABPN1	physical	26496610
CAF1B_HUMAN	CHAF1B	physical	26496610
RAI3_HUMAN	GPRC5A	physical	26496610
MAP7_HUMAN	MAP7	physical	26496610
UBP8_HUMAN	USP8	physical	26496610
VA0D1_HUMAN	ATP6V0D1	physical	26496610
XPR1_HUMAN	XPR1	physical	26496610
ABCG2_HUMAN	ABCG2	physical	26496610
VPS4B_HUMAN	VPS4B	physical	26496610
IST1_HUMAN	IST1	physical	26496610
MVP_HUMAN	MVP	physical	26496610
PDC6I_HUMAN	PDCD6IP	physical	26496610
PDCD6_HUMAN	PDCD6	physical	26496610
CAF1A_HUMAN	CHAF1A	physical	26496610
FLOT1_HUMAN	FLOT1	physical	26496610
ERLN1_HUMAN	ERLIN1	physical	26496610
CKAP4_HUMAN	CKAP4	physical	26496610
BAZ1A_HUMAN	BAZ1A	physical	26496610
PHB2_HUMAN	PHB2	physical	26496610
RIMS1_HUMAN	RIMS1	physical	26496610
MDN1_HUMAN	MDN1	physical	26496610
CD2AP_HUMAN	CD2AP	physical	26496610
PTN23_HUMAN	PTPN23	physical	26496610
CHM2B_HUMAN	CHMP2B	physical	26496610
CHM2A_HUMAN	CHMP2A	physical	26496610
CHM4A_HUMAN	CHMP4A	physical	26496610
RGAP1_HUMAN	RACGAP1	physical	26496610
PACN3_HUMAN	PACSIN3	physical	26496610
PKN3_HUMAN	PKN3	physical	26496610
BAZ2B_HUMAN	BAZ2B	physical	26496610
STML2_HUMAN	STOML2	physical	26496610
CHMP5_HUMAN	CHMP5	physical	26496610
VATH_HUMAN	ATP6V1H	physical	26496610
CHMP3_HUMAN	CHMP3	physical	26496610
S38A2_HUMAN	SLC38A2	physical	26496610
C2D1A_HUMAN	CC2D1A	physical	26496610
BDP1_HUMAN	BDP1	physical	26496610
BI2L1_HUMAN	BAIAP2L1	physical	26496610
BRE1A_HUMAN	RNF20	physical	26496610
CHM1B_HUMAN	CHMP1B	physical	26496610
THOC2_HUMAN	THOC2	physical	26496610
NHSL1_HUMAN	NHSL1	physical	26496610
KDIS_HUMAN	KIDINS220	physical	26496610
CRTC3_HUMAN	CRTC3	physical	26496610
EFL1_HUMAN	EFTUD1	physical	26496610
CYBR1_HUMAN	CYBRD1	physical	26496610
INT5_HUMAN	INTS5	physical	26496610
OBSCN_HUMAN	OBSCN	physical	26496610
PDIP3_HUMAN	POLDIP3	physical	26496610
CC115_HUMAN	CCDC115	physical	26496610
RUSD4_HUMAN	RPUSD4	physical	26496610
YTDC1_HUMAN	YTHDC1	physical	26496610
DPH7_HUMAN	DPH7	physical	26496610
MB12A_HUMAN	MVB12A	physical	26496610
FNIP1_HUMAN	FNIP1	physical	26496610
CAVN3_HUMAN	PRKCDBP	physical	26496610
MITD1_HUMAN	MITD1	physical	26496610
C2D1B_HUMAN	CC2D1B	physical	26496610
CAVN1_HUMAN	PTRF	physical	26496610
TPRN_HUMAN	TPRN	physical	26496610
MICA_HUMAN	MICA	physical	26496610
ACOT1_HUMAN	ACOT1	physical	26496610

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.