GPS2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GPS2_HUMAN
• UniProt AC: Q13227
• Protein Name: G protein pathway suppressor 2 {ECO:0000303|PubMed:19917673, ECO:0000303|PubMed:8943324}
• Gene Name: GPS2 {ECO:0000312|HGNC:HGNC:4550}
• Organism: Homo sapiens (Human).
• Sequence Length: 327
• Subcellular Localization: Nucleus . Mitochondrion . Cytoplasm, cytosol . Sumoylation regulates the subcellular location (PubMed:24943844). Relocates from the mitochondria to the nucleus following desumoylation, leading to mediate mitochondrial stress response (By similarity).
• Protein Description: Key regulator of inflammation, lipid metabolism and mitochondrion homeostasis that acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, thereby inhibiting 'Lys-63'-linked ubiquitination (By similarity). In the nucleus, can both acts as a corepressor and coactivator of transcription, depending on the context. [PubMed: 24943844 Acts as a transcription coactivator in adipocytes by promoting the recruitment of PPARG to promoters: acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, leading to stabilization of KDM4A and subsequent histone H3 'Lys-9' (H3K9) demethylation (By similarity Promotes cholesterol efflux by acting as a transcription coactivator]
• Protein Sequence: MPALLERPKLSNAMARALHRHIMMERERKRQEEEEVDKMMEQKMKEEQERRKKKEMEERMSLEETKEQILKLEEKLLALQEEKHQLFLQLKKVLHEEEKRRRKEQSDLTTLTSAAYQQSLTVHTGTHLLSMQGSPGGHNRPGTLMAADRAKQMFGPQVLTTRHYVGSAAAFAGTPEHGQFQGSPGGAYGTAQPPPHYGPTQPAYSPSQQLRAPSAFPAVQYLSQPQPQPYAVHGHFQPTQTGFLQPGGALSLQKQMEHANQQTGFSDSSSLRPMHPQALHPAPGLLASPQLPVQMQPAGKSGFAATSQPGPRLPFIQHSQNPRFYHK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
45	Sumoylation	KMMEQKMKEEQERRK HHHHHHHHHHHHHHH	66.20	24943844
66	Ubiquitination	RMSLEETKEQILKLE HHCHHHHHHHHHHHH	51.40	21906983
66	Ubiquitination	RMSLEETKEQILKLE HHCHHHHHHHHHHHH	51.40	-
71	Ubiquitination	ETKEQILKLEEKLLA HHHHHHHHHHHHHHH	56.32	22817900
71	Sumoylation	ETKEQILKLEEKLLA HHHHHHHHHHHHHHH	56.32	24943844
71	Ubiquitination	ETKEQILKLEEKLLA HHHHHHHHHHHHHHH	56.32	-
75	2-Hydroxyisobutyrylation	QILKLEEKLLALQEE HHHHHHHHHHHHHHH	39.15	-
75	Ubiquitination	QILKLEEKLLALQEE HHHHHHHHHHHHHHH	39.15	29967540
75	Ubiquitination	QILKLEEKLLALQEE HHHHHHHHHHHHHHH	39.15	-
83	Ubiquitination	LLALQEEKHQLFLQL HHHHHHHHHHHHHHH	35.58	29967540
83	Ubiquitination	LLALQEEKHQLFLQL HHHHHHHHHHHHHHH	35.58	-
83	2-Hydroxyisobutyrylation	LLALQEEKHQLFLQL HHHHHHHHHHHHHHH	35.58	-
91	Ubiquitination	HQLFLQLKKVLHEEE HHHHHHHHHHHHHHH	28.07	29967540
91	2-Hydroxyisobutyrylation	HQLFLQLKKVLHEEE HHHHHHHHHHHHHHH	28.07	-
92	Ubiquitination	QLFLQLKKVLHEEEK HHHHHHHHHHHHHHH	59.63	-
92	Ubiquitination	QLFLQLKKVLHEEEK HHHHHHHHHHHHHHH	59.63	-
119	Phosphorylation	TSAAYQQSLTVHTGT HHHHHHHCCEEECCC	15.52	28348404
121	Phosphorylation	AAYQQSLTVHTGTHL HHHHHCCEEECCCCE	18.58	28348404
124	Phosphorylation	QQSLTVHTGTHLLSM HHCCEEECCCCEEEE	39.17	28348404
126	Phosphorylation	SLTVHTGTHLLSMQG CCEEECCCCEEEECC	15.54	27251275
130	Phosphorylation	HTGTHLLSMQGSPGG ECCCCEEEECCCCCC	18.54	28348404
134	Phosphorylation	HLLSMQGSPGGHNRP CEEEECCCCCCCCCC	11.95	28348404
151	Ubiquitination	LMAADRAKQMFGPQV CCHHHHHHHHHCCCE	43.09	-
151	Ubiquitination	LMAADRAKQMFGPQV CCHHHHHHHHHCCCE	43.09	27667366
161	O-linked_Glycosylation	FGPQVLTTRHYVGSA HCCCEEEECCCCCCH	15.99	30379171
204	Phosphorylation	YGPTQPAYSPSQQLR CCCCCCCCCHHHHCC	28.00	26074081
205	Phosphorylation	GPTQPAYSPSQQLRA CCCCCCCCHHHHCCC	21.85	26074081
207	Phosphorylation	TQPAYSPSQQLRAPS CCCCCCHHHHCCCCC	25.31	26074081
251	Phosphorylation	LQPGGALSLQKQMEH CCCCCHHHHHHHHHH	28.37	24719451
312	Asymmetric dimethylarginine	ATSQPGPRLPFIQHS CCCCCCCCCCCCCCC	61.99	-
312	Methylation	ATSQPGPRLPFIQHS CCCCCCCCCCCCCCC	61.99	24129315
323	Methylation	IQHSQNPRFYHK--- CCCCCCCCCCCC---	52.91	19917673
323	Asymmetric dimethylarginine	IQHSQNPRFYHK--- CCCCCCCCCCCC---	52.91	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of GPS2_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
45	K	Sumoylation	Sumoylated at Lys-45 and Lys-71 in mitochondrion (By similarity).	24943844
45	K	Sumoylation	Sumoylation at Lys-45 and Lys-71 regulates the shuttling between the cytoplasm and the nucleus.	24943844
71	K	Sumoylation	Sumoylated at Lys-45 and Lys-71 in mitochondrion (By similarity).	24943844
71	K	Sumoylation	Sumoylation at Lys-45 and Lys-71 regulates the shuttling between the cytoplasm and the nucleus.	24943844
71	K	Sumoylation	Sumoylation at Lys-71 is required for interaction with TBL1X (By similarity).	24943844
312	R	Methylation	Methylated at Arg-312 and Arg-323 by PRMT6.	-
323	R	Methylation	Interaction with TBL1X and methylation at Arg-323 protect GPS2 against ubiquitination and degradation.	-
323	R	Methylation	Methylated at Arg-312 and Arg-323 by PRMT6.	-
323	R	Methylation	Methylation at Arg-323 protects from degradation by the proteasome.	-
323	R	ubiquitylation	Interaction with TBL1X and methylation at Arg-323 protect GPS2 against ubiquitination and degradation.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GPS2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CS057_HUMAN	C19orf57	physical	16189514
M3KCL_HUMAN	MAP3K7CL	physical	16189514
CCNA1_HUMAN	CCNA1	physical	15159402
NCOR1_HUMAN	NCOR1	physical	11931768
TBL1X_HUMAN	TBL1X	physical	11931768
HDAC3_HUMAN	HDAC3	physical	11931768
EP300_HUMAN	EP300	physical	10846067
GOGA2_HUMAN	GOLGA2	physical	16169070
CHD3_HUMAN	CHD3	physical	16169070
SETB1_HUMAN	SETDB1	physical	16169070
P53_HUMAN	TP53	physical	11486030
NR0B2_HUMAN	NR0B2	physical	17895379
NR0B2_RAT	Nr0b2	physical	17895379
HDAC1_HUMAN	HDAC1	physical	17895379
HDAC3_HUMAN	HDAC3	physical	17895379
NR5A2_HUMAN	NR5A2	physical	17895379
HNF4A_HUMAN	HNF4A	physical	17895379
NR1H4_HUMAN	NR1H4	physical	17895379
MSH4_HUMAN	MSH4	physical	16122992
MSH5_HUMAN	MSH5	physical	16122992
TBL1X_HUMAN	TBL1X	physical	24943844
NCOR2_HUMAN	NCOR2	physical	24943844
HDAC3_HUMAN	HDAC3	physical	24943844
K1H1_HUMAN	KRT31	physical	25416956
RBP1_HUMAN	RALBP1	physical	25416956
RBPMS_HUMAN	RBPMS	physical	25416956
TFP11_HUMAN	TFIP11	physical	25416956
SESD1_HUMAN	SESTD1	physical	25416956
TBL1R_HUMAN	TBL1XR1	physical	26186194
TBL1X_HUMAN	TBL1X	physical	26186194
NCOR1_HUMAN	NCOR1	physical	26186194
NCOR2_HUMAN	NCOR2	physical	26186194
LIPA1_HUMAN	PPFIA1	physical	26186194
LIPA3_HUMAN	PPFIA3	physical	26186194
MK03_HUMAN	MAPK3	physical	26186194
ARMC8_HUMAN	ARMC8	physical	26186194
MKLN1_HUMAN	MKLN1	physical	26186194
MAEA_HUMAN	MAEA	physical	26186194
DCTN4_HUMAN	DCTN4	physical	26186194
RMD5A_HUMAN	RMND5A	physical	26186194
RBP10_HUMAN	RANBP10	physical	26186194
ANR11_HUMAN	ANKRD11	physical	26186194
CYH3_HUMAN	CYTH3	physical	26186194
GID4_HUMAN	GID4	physical	26186194
ARP10_HUMAN	ACTR10	physical	26186194
DAPK3_HUMAN	DAPK3	physical	26186194
TRI68_HUMAN	TRIM68	physical	26186194
GID8_HUMAN	GID8	physical	26186194
FOXK2_HUMAN	FOXK2	physical	26186194
FOXK1_HUMAN	FOXK1	physical	26186194
CREB1_HUMAN	CREB1	physical	26186194
DCTN6_HUMAN	DCTN6	physical	26186194
DCTN3_HUMAN	DCTN3	physical	26186194
YPEL5_HUMAN	YPEL5	physical	26186194
HDAC3_HUMAN	HDAC3	physical	26186194
TBL1X_HUMAN	TBL1X	physical	26070566
GPA1_YEAST	GPA1	genetic	8943324
GBB_YEAST	STE4	genetic	8943324
MK08_HUMAN	MAPK8	genetic	8943324
ANR11_HUMAN	ANKRD11	physical	28514442
NCOR2_HUMAN	NCOR2	physical	28514442
LIPA3_HUMAN	PPFIA3	physical	28514442
TBL1X_HUMAN	TBL1X	physical	28514442
DAPK3_HUMAN	DAPK3	physical	28514442
NCOR1_HUMAN	NCOR1	physical	28514442
LIPA1_HUMAN	PPFIA1	physical	28514442
TBL1R_HUMAN	TBL1XR1	physical	28514442
HDAC3_HUMAN	HDAC3	physical	28514442
CYH3_HUMAN	CYTH3	physical	28514442
DCTN6_HUMAN	DCTN6	physical	28514442
TRI68_HUMAN	TRIM68	physical	28514442
FOXK1_HUMAN	FOXK1	physical	28514442
GID4_HUMAN	GID4	physical	28514442
FOXK2_HUMAN	FOXK2	physical	28514442
ARP10_HUMAN	ACTR10	physical	28514442
MK03_HUMAN	MAPK3	physical	28514442
ARMC8_HUMAN	ARMC8	physical	28514442
DCTN4_HUMAN	DCTN4	physical	28514442
DCTN3_HUMAN	DCTN3	physical	28514442
MKLN1_HUMAN	MKLN1	physical	28514442
CREB1_HUMAN	CREB1	physical	28514442
GID8_HUMAN	GID8	physical	28514442
ACTY_HUMAN	ACTR1B	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.