NR5A2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NR5A2_HUMAN
• UniProt AC: O00482
• Protein Name: Nuclear receptor subfamily 5 group A member 2
• Gene Name: NR5A2
• Organism: Homo sapiens (Human).
• Sequence Length: 541
• Subcellular Localization: Nucleus .
• Protein Description: Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. [PubMed: 20159957 Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.]
• Protein Sequence: MSSNSDTGDLQESLKHGLTPIGAGLPDRHGSPIPARGRLVMLPKVETEALGLARSHGEQGQMPENMQVSQFKMVNYSYDEDLEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKRYTCIENQNCQIDKTQRKRCPYCRFQKCLSVGMKLEAVRADRMRGGRNKFGPMYKRDRALKQQKKALIRANGLKLEAMSQVIQAMPSDLTISSAIQNIHSASKGLPLNHAALPPTDYDRSPFVTSPISMTMPPHGSLQGYQTYGHFPSRAIKSEYPDPYTSSPESIMGYSYMDSYQTSSPASIPHLILELLKCEPDEPQVQAKIMAYLQQEQANRSKHEKLSTFGLMCKMADQTLFSIVEWARSSIFFRELKVDDQMKLLQNCWSELLILDHIYRQVVHGKEGSIFLVTGQQVDYSIIASQAGATLNNLMSHAQELVAKLRSLQFDQREFVCLKFLVLFSLDVKNLENFQLVEGVQEQVNAALLDYTMCNYPQQTEKFGQLLLRLPEIRAISMQAEEYLYYKHLNGDVPYNNLLIEMLHAKRA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSSNSDTGD ------CCCCCCCCC	37.92	26503514
3	Phosphorylation	-----MSSNSDTGDL -----CCCCCCCCCH	38.77	26503514
13	Phosphorylation	DTGDLQESLKHGLTP CCCCHHHHHHCCCCC	29.74	26503514
31	Phosphorylation	GLPDRHGSPIPARGR CCCCCCCCCCCCCCC	17.33	22798277
107	Acetylation	LLTCESCKGFFKRTV EEEHHHCCCHHHHHH	68.95	11479297
111	Acetylation	ESCKGFFKRTVQNNK HHCCCHHHHHHHCCC	44.92	11479297
113	Phosphorylation	CKGFFKRTVQNNKRY CCCHHHHHHHCCCEE	27.34	-
173	"N6,N6-dimethyllysine"	NKFGPMYKRDRALKQ CCCCHHHHHHHHHHH	41.38	-
173	Sumoylation	NKFGPMYKRDRALKQ CCCCHHHHHHHHHHH	41.38	-
173	Methylation	NKFGPMYKRDRALKQ CCCCHHHHHHHHHHH	41.38	-
238	Phosphorylation	PPTDYDRSPFVTSPI CCCCCCCCCCCCCCC	21.57	21614002
243	Phosphorylation	DRSPFVTSPISMTMP CCCCCCCCCCCCCCC	18.22	21614002
270	Sumoylation	HFPSRAIKSEYPDPY CCCCCHHCCCCCCCC	35.93	-
270	Sumoylation	HFPSRAIKSEYPDPY CCCCCHHCCCCCCCC	35.93	20159957
383	Phosphorylation	KLLQNCWSELLILDH HHHHHHHHHHHHHHH	22.14	-
510	Phosphorylation	LPEIRAISMQAEEYL CHHHHHHEECHHHHH	12.12	22817900

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
510	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
-	K	Ubiquitination	E3 ubiquitin ligase	DDB1	Q16531	PMID:30923324

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
270	K	Sumoylation	Sumoylated by SUMO1 at Lys-270 during the hepatic acute phase response, leading to promote interaction with GPS2 and prevent N-Cor corepressor complex dissociation.	20159957

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NR5A2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NR0B2_HUMAN	NR0B2	physical	12198243
PTN6_MOUSE	Ptpn6	physical	11668176
NR0B2_HUMAN	NR0B2	physical	20375098
NR0B2_HUMAN	NR0B2	physical	20516075
ALBU_HUMAN	ALB	physical	15604093
FRIH_HUMAN	FTH1	physical	15604093
NRIP1_HUMAN	NRIP1	physical	15604093
BCKD_HUMAN	BCKDK	physical	15604093
ANF_HUMAN	NPPA	physical	15604093
PNRC1_HUMAN	PNRC1	physical	15604093
PNRC2_HUMAN	PNRC2	physical	15604093
NR0B2_HUMAN	NR0B2	physical	21566081
ILF3_HUMAN	ILF3	physical	21554248
CREB1_HUMAN	CREB1	physical	18191017
NCOA1_HUMAN	NCOA1	physical	23975195
NCOA1_HUMAN	NCOA1	physical	15604093

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.