HUWE1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HUWE1_MOUSE
• UniProt AC: Q7TMY8
• Protein Name: E3 ubiquitin-protein ligase HUWE1
• Gene Name: Huwe1
• Organism: Mus musculus (Mouse).
• Sequence Length: 4377
• Subcellular Localization: Cytoplasm . Nucleus . Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells.
• Protein Description: E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene (By similarity). Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of PA2G4 (By similarity)..
• Protein Sequence: MKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEKEQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPSTTQEGEEMETDMDGVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEEAGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIIGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQCTVTKTNDSTEQNVSDGTPMPDSYPTTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTPAAGSSEQPTAGSSTPGDAPSVVAEVQGRPDVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSGGEGETSPHSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEVQTNSSNSGSSTAATSNTSTTTTTTTTATAPTPTPPAATTPVTSAPALVAATAISTITVAASTTVTTPTTATTTVSTSTTKGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
25	Ubiquitination	DCRALIDKLKVCNDE HHHHHHHHHCCCCHH	43.12	-
52	Phosphorylation	NIGKCELYHWVDLLD CCCHHHHHHHHHHHH	3.26	19367708
153	Phosphorylation	NYITRLGSDKRTPLL CCHHHCCCCCCCHHH	44.54	21183079
233	Phosphorylation	IEQLDKISESPSEIM HHHHHHHCCCHHHHH	37.45	29472430
235	Phosphorylation	QLDKISESPSEIMES HHHHHCCCHHHHHHH	27.25	29472430
237	Phosphorylation	DKISESPSEIMESLT HHHCCCHHHHHHHHH	49.38	29472430
242	Phosphorylation	SPSEIMESLTKMYSI CHHHHHHHHHHHHCC	24.78	29472430
244	Phosphorylation	SEIMESLTKMYSIPK HHHHHHHHHHHCCCC	24.04	29472430
324	Ubiquitination	DKQLMEIKAASLRTL CHHHHHHHHHHHHHH	25.29	-
342	Ubiquitination	VHLERTPKLSSIIDC HHHHCCCCCHHCEEC	61.46	-
483	Phosphorylation	KPKIQRPSTTQEGEE CCCCCCCCCCCCCCC	46.40	23684622
632	Ubiquitination	QPFERLFKVLLSPDY CCHHHHHHHHHCCCC	36.24	-
648	Phosphorylation	PAMRRRRSSDPLGDT HHHHHHCCCCCCCHH	36.96	25521595
649	Phosphorylation	AMRRRRSSDPLGDTA HHHHHCCCCCCCHHH	41.24	25521595
655	Phosphorylation	SSDPLGDTASNLGSA CCCCCCHHHHHHHHH	30.13	23984901
657	Phosphorylation	DPLGDTASNLGSAVD CCCCHHHHHHHHHHH	34.38	25619855
661	Phosphorylation	DTASNLGSAVDELMR HHHHHHHHHHHHHHH	28.43	25777480
674	Ubiquitination	MRHQPTLKTDATTAI HHCCCCCCCHHHHHH	47.00	-
718	Phosphorylation	ATAPPPRSNHAAEEA CCCCCCCCCCCHHHC	38.45	22802335
726	Phosphorylation	NHAAEEASSEDEEEE CCCHHHCCCCCHHHH	37.22	19060867
727	Phosphorylation	HAAEEASSEDEEEEE CCHHHCCCCCHHHHH	56.99	19060867
740	Phosphorylation	EEVQAMQSFNSAQQN HHHHHHHHHHHHHCC	17.20	-
830	Phosphorylation	AVAGVCKSILTLSHE HHHHHHHHHHHHCCC	20.08	-
977	Acetylation	DMQKLVPKDEKAGTT HHHHHCCCCCCCCCC	71.66	15613353
1077	Phosphorylation	LVKLCVGSPVRQRRS HHHHHCCCHHHHCCC	10.38	20415495
1084	Phosphorylation	SPVRQRRSHHAASTT CHHHHCCCCCCCCCC	23.08	26824392
1089	Phosphorylation	RRSHHAASTTTAPTP CCCCCCCCCCCCCCH	27.37	29895711
1090	Phosphorylation	RSHHAASTTTAPTPA CCCCCCCCCCCCCHH	24.36	29895711
1091	Phosphorylation	SHHAASTTTAPTPAA CCCCCCCCCCCCHHH	20.61	18846507
1092	Phosphorylation	HHAASTTTAPTPAAR CCCCCCCCCCCHHHH	30.63	18846507
1095	Phosphorylation	ASTTTAPTPAARSTA CCCCCCCCHHHHHHH	24.09	18846507
1107	Ubiquitination	STASALTKLLTKGLS HHHHHHHHHHHCCCC	42.20	-
1111	Ubiquitination	ALTKLLTKGLSWQPP HHHHHHHCCCCCCCC	58.80	-
1298	Phosphorylation	LSKEKEGSRGEEEAG HHHCCCCCCCCHHHC	37.90	26824392
1342	Phosphorylation	AMEALLNTSTMEQAT HHHHHHCCCCHHHHH	26.08	26643407
1343	Phosphorylation	MEALLNTSTMEQATE HHHHHCCCCHHHHHH	25.36	26643407
1344	Phosphorylation	EALLNTSTMEQATEY HHHHCCCCHHHHHHH	23.76	26643407
1349	Phosphorylation	TSTMEQATEYLLTHP CCCHHHHHHHHHHCC	25.51	26643407
1368	Phosphorylation	GGVVRDLSMSEEDQM CHHHCCCCCCHHHHH	25.26	25521595
1370	Phosphorylation	VVRDLSMSEEDQMMR HHCCCCCCHHHHHHH	34.16	28833060
1382	Phosphorylation	MMRAIAMSLGQDIPM HHHHHHHHCCCCCCC	21.96	-
1395	Phosphorylation	PMDQRAESPEEVACR CCHHCCCCHHHHHHC	36.30	25521595
1403	Ubiquitination	PEEVACRKEEEERKA HHHHHHCHHHHHHHH	70.16	-
1470	Ubiquitination	DLIMTAIKRNGADYR HHHHHHHHHCCCCHH	37.03	-
1482	Acetylation	DYRDMILKQVVNQVW CHHHHHHHHHHHHHH	30.07	7923559
1608	Ubiquitination	KRRAQMTKYLQSNSN HHHHHHHHHHHHCCC	38.32	-
1643	Ubiquitination	STIDSAWKSGETSVR CCCCCCHHCCCCEEE	48.58	-
1663	Phosphorylation	RRYTVQFTTMVQVNE EEEEEEEEEEEEECC	9.26	30635358
1664	Phosphorylation	RYTVQFTTMVQVNEE EEEEEEEEEEEECCC	19.34	30635358
1692	Phosphorylation	VPRLSKNSKSSNGQE CCCCCCCCCCCCHHH	37.34	26160508
1693	Ubiquitination	PRLSKNSKSSNGQEL CCCCCCCCCCCHHHH	68.26	-
1694	Phosphorylation	RLSKNSKSSNGQELE CCCCCCCCCCHHHHH	28.93	25266776
1695	Phosphorylation	LSKNSKSSNGQELEK CCCCCCCCCHHHHHH	49.03	18779572
1703	Phosphorylation	NGQELEKTLEESKET CHHHHHHHHHHHHHH	29.99	26160508
1707	Phosphorylation	LEKTLEESKETDIKH HHHHHHHHHHHHCCC	26.81	26160508
1728	Phosphorylation	DIPLALESTNTEKEA CCCEEEECCCCCCCC	28.01	30635358
1729	Phosphorylation	IPLALESTNTEKEAS CCEEEECCCCCCCCC	36.46	30635358
1731	Phosphorylation	LALESTNTEKEASLD EEEECCCCCCCCCCC	48.82	30635358
1733	Ubiquitination	LESTNTEKEASLDET EECCCCCCCCCCCHH	57.45	-
1736	Phosphorylation	TNTEKEASLDETKIG CCCCCCCCCCHHHHH	37.13	28066266
1740	Phosphorylation	KEASLDETKIGEILI CCCCCCHHHHHHHHH	27.85	28066266
1840	Ubiquitination	TLRHTMEKVVRSAAT HHHHHHHHHHHHHHH	34.90	-
1903	Phosphorylation	ALPAPRGSGTASDDE ECCCCCCCCCCCHHH	33.96	25521595
1905	Phosphorylation	PAPRGSGTASDDEFE CCCCCCCCCCHHHHH	25.37	24925903
1907	Phosphorylation	PRGSGTASDDEFENL CCCCCCCCHHHHHCC	45.49	24925903
1917	Ubiquitination	EFENLRIKGPNAVQL HHHCCEECCCCCEEE	62.98	-
1927	Phosphorylation	NAVQLVKTTPLKPSS CCEEEEECCCCCCCC	26.60	26643407
1928	Phosphorylation	AVQLVKTTPLKPSSL CEEEEECCCCCCCCC	22.39	26643407
1931	Acetylation	LVKTTPLKPSSLPVI EEECCCCCCCCCCCC	44.02	23236377
1934	Phosphorylation	TTPLKPSSLPVIPDT CCCCCCCCCCCCCHH	45.26	26643407
1999	Phosphorylation	RSLTRQSSDFDTQSG HHHHCCCCCCCCCCC	33.68	-
2035	Phosphorylation	TSQGEASTPEETREG CCCCCCCCHHHHCCC	41.59	-
2051	Phosphorylation	KDKEGDRTSEEGKQK CCCCCCCCCHHHHHC	45.27	29899451
2172	Ubiquitination	AMAESTEKHARLQAV HHHHHHHHHHHHHHH	42.53	-
2189	Phosphorylation	IISTIMESCPSTSSF HHHHHHHHCCCCHHH	17.59	22802335
2192	Phosphorylation	TIMESCPSTSSFYSS HHHHHCCCCHHHCCC	45.29	22802335
2193	Phosphorylation	IMESCPSTSSFYSSA HHHHCCCCHHHCCCC	17.34	22802335
2194	Phosphorylation	MESCPSTSSFYSSAT HHHCCCCHHHCCCCC	23.84	22802335
2195	Phosphorylation	ESCPSTSSFYSSATA HHCCCCHHHCCCCCC	28.43	22802335
2197	Phosphorylation	CPSTSSFYSSATAKT CCCCHHHCCCCCCCC	12.03	22802335
2198	Phosphorylation	PSTSSFYSSATAKTQ CCCHHHCCCCCCCCC	16.10	22802335
2199	Phosphorylation	STSSFYSSATAKTQH CCHHHCCCCCCCCCC	20.47	22802335
2201	Phosphorylation	SSFYSSATAKTQHNG HHHCCCCCCCCCCCC	30.54	22802335
2204	Phosphorylation	YSSATAKTQHNGMNN CCCCCCCCCCCCHHH	32.37	22802335
2219	Ubiquitination	IIRLFLKKGLVNDLA HHHHHHHCCCHHHHH	60.67	-
2248	Acetylation	NTVNAALKPLETLSR HHHHHHHHHHHHHHH	42.71	23236377
2248	Ubiquitination	NTVNAALKPLETLSR HHHHHHHHHHHHHHH	42.71	-
2261	Phosphorylation	SRIVNQPSSLFGSKS HHHHCCCHHHHCCCC	29.88	28066266
2262	Phosphorylation	RIVNQPSSLFGSKSA HHHCCCHHHHCCCCC	34.11	20415495
2266	Phosphorylation	QPSSLFGSKSASSKN CCHHHHCCCCCCCCC	18.99	20415495
2267	Acetylation	PSSLFGSKSASSKNK CHHHHCCCCCCCCCC	50.88	23806337
2267	Ubiquitination	PSSLFGSKSASSKNK CHHHHCCCCCCCCCC	50.88	-
2362	Phosphorylation	LLERDGGSGNSTIIV HHHHCCCCCCCEEEE	40.09	25521595
2365	Phosphorylation	RDGGSGNSTIIVSRS HCCCCCCCEEEEECC	25.22	26824392
2366	Phosphorylation	DGGSGNSTIIVSRSG CCCCCCCEEEEECCC	20.79	25521595
2370	Phosphorylation	GNSTIIVSRSGEDES CCCEEEEECCCCCCC	15.43	25619855
2372	Phosphorylation	STIIVSRSGEDESQE CEEEEECCCCCCCHH	38.74	25293948
2377	Phosphorylation	SRSGEDESQEDVLMD ECCCCCCCHHHHCCC	51.71	25521595
2388	Phosphorylation	VLMDEAPSNLSQAST HCCCCCCCCHHHHHH	58.41	25293948
2391	Phosphorylation	DEAPSNLSQASTLQA CCCCCCHHHHHHHHC	28.13	25293948
2394	Phosphorylation	PSNLSQASTLQANRE CCCHHHHHHHHCCCH	23.24	25293948
2395	Phosphorylation	SNLSQASTLQANRED CCHHHHHHHHCCCHH	26.38	25293948
2500	Phosphorylation	IEFDNMFSSATDIPP EEECCCCCCCCCCCC	14.32	26643407
2501	Phosphorylation	EFDNMFSSATDIPPS EECCCCCCCCCCCCC	24.75	26643407
2503	Phosphorylation	DNMFSSATDIPPSPG CCCCCCCCCCCCCCC	36.09	26643407
2508	Phosphorylation	SATDIPPSPGNIPTT CCCCCCCCCCCCCCC	39.83	26643407
2514	Phosphorylation	PSPGNIPTTHPLMVR CCCCCCCCCCCEEEE	33.66	26643407
2515	Phosphorylation	SPGNIPTTHPLMVRH CCCCCCCCCCEEEEE	19.01	26643407
2526	Phosphorylation	MVRHADHSSLTLGSG EEEECCCCCEECCCC	27.82	25266776
2527	Phosphorylation	VRHADHSSLTLGSGS EEECCCCCEECCCCC	23.19	25266776
2529	Phosphorylation	HADHSSLTLGSGSST ECCCCCEECCCCCCH	30.90	25266776
2532	Phosphorylation	HSSLTLGSGSSTTRL CCCEECCCCCCHHHC	38.32	28507225
2534	Phosphorylation	SLTLGSGSSTTRLTQ CEECCCCCCHHHCCC	26.63	25266776
2535	Phosphorylation	LTLGSGSSTTRLTQG EECCCCCCHHHCCCC	37.01	28066266
2536	Phosphorylation	TLGSGSSTTRLTQGI ECCCCCCHHHCCCCC	20.03	28066266
2537	Phosphorylation	LGSGSSTTRLTQGIG CCCCCCHHHCCCCCC	26.15	28066266
2540	Phosphorylation	GSSTTRLTQGIGRSQ CCCHHHCCCCCCCCH	22.81	22006019
2546	Phosphorylation	LTQGIGRSQRTLRQL CCCCCCCCHHHHHHH	20.85	25266776
2549	Phosphorylation	GIGRSQRTLRQLTAN CCCCCHHHHHHHHCC	20.75	29514104
2554	Phosphorylation	QRTLRQLTANTGHTI HHHHHHHHCCCCCEE	14.99	25338131
2557	Phosphorylation	LRQLTANTGHTIHVH HHHHHCCCCCEEEEE	28.07	28285833
2560	Phosphorylation	LTANTGHTIHVHYPG HHCCCCCEEEEECCC	17.70	28285833
2584	Phosphorylation	LQRLLGPSAAADILQ HHHHHCHHHHHHHHH	28.83	-
2593	Phosphorylation	AADILQLSSSLPLQS HHHHHHHHCCCCCCC	12.62	26745281
2594	Phosphorylation	ADILQLSSSLPLQSR HHHHHHHCCCCCCCC	44.48	26745281
2595	Phosphorylation	DILQLSSSLPLQSRG HHHHHHCCCCCCCCC	30.54	26824392
2600	Phosphorylation	SSSLPLQSRGRARLL HCCCCCCCCCCEEEE	44.37	26745281
2619	Phosphorylation	DVHIIARSDDELLDD CEEEEECCCCHHHHH	40.15	27180971
2632	Phosphorylation	DDFFHDQSTATSQAG HHHCCCCCCCCCCCC	26.52	25293948
2633	Phosphorylation	DFFHDQSTATSQAGT HHCCCCCCCCCCCCC	28.46	25293948
2635	Phosphorylation	FHDQSTATSQAGTLS CCCCCCCCCCCCCCH	22.81	21183079
2636	Phosphorylation	HDQSTATSQAGTLSS CCCCCCCCCCCCCHH	18.64	25293948
2671	Ubiquitination	HDCVSVVKVPIVNHL CCCEEEEECCCCCHH	39.89	-
2695	Ubiquitination	ERREKRRKQLAEEET HHHHHHHHHHHHHHH	54.41	-
2703	Ubiquitination	QLAEEETKIIDKGKE HHHHHHHHHHHCCHH	40.73	-
2709	Ubiquitination	TKIIDKGKEDKENRD HHHHHCCHHCCCCCC	69.36	-
2712	Acetylation	IDKGKEDKENRDQSA HHCCHHCCCCCCCCC	58.96	7610827
2726	Phosphorylation	AQCTVTKTNDSTEQN CCEEEEECCCCCCCC	34.59	25777480
2729	Phosphorylation	TVTKTNDSTEQNVSD EEEECCCCCCCCCCC	35.59	25777480
2730	Phosphorylation	VTKTNDSTEQNVSDG EEECCCCCCCCCCCC	44.44	25777480
2735	Phosphorylation	DSTEQNVSDGTPMPD CCCCCCCCCCCCCCC	37.56	26643407
2738	Phosphorylation	EQNVSDGTPMPDSYP CCCCCCCCCCCCCCC	22.77	25777480
2743	Phosphorylation	DGTPMPDSYPTTPSS CCCCCCCCCCCCCCC	27.43	26643407
2744	Phosphorylation	GTPMPDSYPTTPSST CCCCCCCCCCCCCCC	16.39	26643407
2746	Phosphorylation	PMPDSYPTTPSSTDA CCCCCCCCCCCCCCC	43.01	25521595
2747	Phosphorylation	MPDSYPTTPSSTDAP CCCCCCCCCCCCCCC	19.37	26643407
2749	Phosphorylation	DSYPTTPSSTDAPTS CCCCCCCCCCCCCCC	43.72	29899451
2750	Phosphorylation	SYPTTPSSTDAPTSE CCCCCCCCCCCCCCC	31.35	26643407
2751	Phosphorylation	YPTTPSSTDAPTSES CCCCCCCCCCCCCCC	40.28	29899451
2755	Phosphorylation	PSSTDAPTSESKETL CCCCCCCCCCCHHHH	47.01	25777480
2756	Phosphorylation	SSTDAPTSESKETLG CCCCCCCCCCHHHHC	38.52	25777480
2758	Phosphorylation	TDAPTSESKETLGTL CCCCCCCCHHHHCCC	35.24	25777480
2820	Phosphorylation	RPSGEAETTQMELSP CCCCCCCCCCEECCC	29.40	28066266
2821	Phosphorylation	PSGEAETTQMELSPA CCCCCCCCCEECCCC	19.88	28066266
2826	Phosphorylation	ETTQMELSPAPTITS CCCCEECCCCCCCCC	13.00	28066266
2830	Phosphorylation	MELSPAPTITSLSPE EECCCCCCCCCCCHH	38.71	28066266
2832	Phosphorylation	LSPAPTITSLSPERA CCCCCCCCCCCHHHC	26.54	28066266
2833	Phosphorylation	SPAPTITSLSPERAE CCCCCCCCCCHHHCC	24.01	28066266
2835	Phosphorylation	APTITSLSPERAEDS CCCCCCCCHHHCCCC	25.20	28066266
2861	Phosphorylation	GSPMDTSSLASCTLE CCCCCHHHHHHCCHH	30.01	-
2887	Phosphorylation	SEQPTAGSSTPGDAP CCCCCCCCCCCCCCC	28.68	-
2888	Phosphorylation	EQPTAGSSTPGDAPS CCCCCCCCCCCCCCC	37.39	-
2889	Phosphorylation	QPTAGSSTPGDAPSV CCCCCCCCCCCCCCH	32.75	-
2910	Phosphorylation	RPDVSRESNQPPEDS CCCCCCCCCCCCCCC	39.39	25619855
2917	Phosphorylation	SNQPPEDSSPPASSE CCCCCCCCCCCCCCC	42.98	23684622
2918	Phosphorylation	NQPPEDSSPPASSES CCCCCCCCCCCCCCC	47.42	25521595
2922	Phosphorylation	EDSSPPASSESSSTR CCCCCCCCCCCCCCH	40.17	25619855
2923	Phosphorylation	DSSPPASSESSSTRD CCCCCCCCCCCCCHH	43.53	25619855
2925	Phosphorylation	SPPASSESSSTRDSA CCCCCCCCCCCHHHC	31.38	25619855
2926	Phosphorylation	PPASSESSSTRDSAV CCCCCCCCCCHHHCE	31.51	25619855
2927	Phosphorylation	PASSESSSTRDSAVA CCCCCCCCCHHHCEE	36.42	25619855
2928	Phosphorylation	ASSESSSTRDSAVAI CCCCCCCCHHHCEEE	40.21	25619855
2931	Phosphorylation	ESSSTRDSAVAISGA CCCCCHHHCEEECCC	22.73	25338131
2936	Phosphorylation	RDSAVAISGADSRGI HHHCEEECCCCCCCC	20.42	28066266
2940	Phosphorylation	VAISGADSRGILEEP EEECCCCCCCCCCCC	31.69	28066266
2950	Phosphorylation	ILEEPLPSTSSEEED CCCCCCCCCCCCCCC	48.91	26239621
2951	Phosphorylation	LEEPLPSTSSEEEDP CCCCCCCCCCCCCCC	34.04	26239621
2952	Phosphorylation	EEPLPSTSSEEEDPL CCCCCCCCCCCCCCC	39.84	26239621
2953	Phosphorylation	EPLPSTSSEEEDPLA CCCCCCCCCCCCCCC	49.46	26239621
2963	Phosphorylation	EDPLAGISLPEGVDP CCCCCCCCCCCCCCH	36.63	23649490
2995	Phosphorylation	QLGIRPPTRSAPSSN CCCCCCCCCCCCCCC	39.68	-
3060	Phosphorylation	DTPMDPVTFIQTLPS CCCCCHHHHHHCCCH	22.02	-
3072	Phosphorylation	LPSDLRRSVLEDMED CCHHHHHHHHHHCCC	25.31	-
3116	Phosphorylation	HERLFGHSSTSALSA HHHHHCCCHHHHHHH	35.37	26824392
3117	Phosphorylation	ERLFGHSSTSALSAI HHHHCCCHHHHHHHH	22.25	19060867
3118	Phosphorylation	RLFGHSSTSALSAIL HHHCCCHHHHHHHHH	22.49	25293948
3119	Phosphorylation	LFGHSSTSALSAILR HHCCCHHHHHHHHHC	29.21	29472430
3122	Phosphorylation	HSSTSALSAILRSPA CCHHHHHHHHHCCHH	16.99	26239621
3127	Phosphorylation	ALSAILRSPAFTSRL HHHHHHCCHHHHHHC	19.69	26824392
3131	Phosphorylation	ILRSPAFTSRLSGNR HHCCHHHHHHCCCCC	18.29	20139300
3132	Phosphorylation	LRSPAFTSRLSGNRG HCCHHHHHHCCCCCC	25.44	26239621
3135	Phosphorylation	PAFTSRLSGNRGVQY HHHHHHCCCCCCCCE	32.67	25266776
3142	Phosphorylation	SGNRGVQYTRLAVQR CCCCCCCEEEEEEEC	7.45	29514104
3143	Phosphorylation	GNRGVQYTRLAVQRG CCCCCCEEEEEEECC	11.34	20139300
3149	Methylation	YTRLAVQRGGTFQMG EEEEEEECCCCEECC	38.62	24129315
3158	Phosphorylation	GTFQMGGSSSHNRPS CCEECCCCCCCCCCC	23.96	-
3171	Phosphorylation	PSGSNVDTLLRLRGR CCCCCHHHHHHHHCH	24.33	25338131
3244	Ubiquitination	ELCIETPKLSTSEER CCEECCCCCCCCHHH	63.59	-
3247	Phosphorylation	IETPKLSTSEERGKK ECCCCCCCCHHHCCC	51.06	28066266
3248	Phosphorylation	ETPKLSTSEERGKKS CCCCCCCCHHHCCCC	33.80	28066266
3317	Phosphorylation	KHTEKHASSGSTVHI CCCCCCCCCCCEEEE	34.64	23684622
3318	Phosphorylation	HTEKHASSGSTVHIH CCCCCCCCCCEEEEC	37.23	23684622
3345	Ubiquitination	DTLIQLAKVFPSHFT HHHHHHHHHCHHHHC	53.66	-
3357	Ubiquitination	HFTQQRTKETNCESD HHCCHHHCCCCCHHH	65.81	-
3363	Phosphorylation	TKETNCESDRERGSK HCCCCCHHHHHCCCC	44.14	23684622
3374	Phosphorylation	RGSKQACSPCSSQSS CCCCCCCCCCCCCCC	31.77	25266776
3377	Phosphorylation	KQACSPCSSQSSSSG CCCCCCCCCCCCCCC	34.49	26745281
3378	Phosphorylation	QACSPCSSQSSSSGI CCCCCCCCCCCCCCC	40.58	23984901
3380	Phosphorylation	CSPCSSQSSSSGICT CCCCCCCCCCCCCCH	33.85	23984901
3381	Phosphorylation	SPCSSQSSSSGICTD CCCCCCCCCCCCCHH	22.38	23649490
3382	Phosphorylation	PCSSQSSSSGICTDF CCCCCCCCCCCCHHH	37.52	23649490
3383	Phosphorylation	CSSQSSSSGICTDFW CCCCCCCCCCCHHHH	33.84	23649490
3408	Phosphorylation	VSRKGKNSVKSVPVS CCCCCCCCCEECCCC	34.11	25777480
3411	Phosphorylation	KGKNSVKSVPVSSGG CCCCCCEECCCCCCC	29.51	25777480
3415	Phosphorylation	SVKSVPVSSGGEGET CCEECCCCCCCCCCC	19.73	25777480
3416	Phosphorylation	VKSVPVSSGGEGETS CEECCCCCCCCCCCC	51.56	25777480
3422	Phosphorylation	SSGGEGETSPHSLEA CCCCCCCCCCCCCCC	59.42	25777480
3423	Phosphorylation	SGGEGETSPHSLEAS CCCCCCCCCCCCCCC	19.13	25777480
3426	Phosphorylation	EGETSPHSLEASPLG CCCCCCCCCCCCHHH	31.15	25777480
3430	Phosphorylation	SPHSLEASPLGQLMN CCCCCCCCHHHHHHH	15.98	25777480
3440	Phosphorylation	GQLMNMLSHPVIRRS HHHHHHHCCHHHHCH	18.25	25777480
3447	Phosphorylation	SHPVIRRSSLLTEKL CCHHHHCHHHHHHHH	18.58	29176673
3448	Phosphorylation	HPVIRRSSLLTEKLL CHHHHCHHHHHHHHH	26.11	19854140
3453	Ubiquitination	RSSLLTEKLLRLLSL CHHHHHHHHHHHHHH	47.81	-
3555	Phosphorylation	STSTTKGSKSPAKVG ECCCCCCCCCCCCCC	31.36	25619855
3556	Phosphorylation	TSTTKGSKSPAKVGE CCCCCCCCCCCCCCC	69.95	24719451
3557	Phosphorylation	STTKGSKSPAKVGEG CCCCCCCCCCCCCCC	31.61	23684622
3566	Phosphorylation	AKVGEGGSGIDFKMV CCCCCCCCCCCEEEE	43.35	25619855
3622	Ubiquitination	GTRDTVLKLLLNGAR CCHHHHHHHHHHHHH	32.52	-
3637	Ubiquitination	HLGYTLCKQIGTLLA HHHHHHHHHHHHHHH	48.33	-
3661	Phosphorylation	QRRAQCETLSPDGLP HHHHHCCCCCCCCCC	39.91	24925903
3663	Phosphorylation	RAQCETLSPDGLPEE HHHCCCCCCCCCCCC	28.50	25521595
3674	Phosphorylation	LPEEQPQTTKLKGKM CCCCCCCCCCCCCHH	32.36	25619855
3675	Phosphorylation	PEEQPQTTKLKGKMQ CCCCCCCCCCCCHHH	29.18	25619855
3676	Ubiquitination	EEQPQTTKLKGKMQS CCCCCCCCCCCHHHH	52.14	-
3678	Ubiquitination	QPQTTKLKGKMQSRF CCCCCCCCCHHHHCC	59.03	-
3698	Ubiquitination	VVIVASQKRPLGGRE EEEEEECCCCCCCCC	54.19	-
3717	Ubiquitination	SMSMLTSKTSTQKFF CHHHHCCCCHHHHHH	41.06	-
3722	Ubiquitination	TSKTSTQKFFLRVLQ CCCCHHHHHHHHHHH	37.87	-
3753	Phosphorylation	KQTGRLGSSGLGSAS HHHCCCCCCCCCCHH	26.31	26824392
3754	Phosphorylation	QTGRLGSSGLGSASS HHCCCCCCCCCCHHH	35.92	22942356
3758	Phosphorylation	LGSSGLGSASSIQAA CCCCCCCCHHHHHHH	29.98	25521595
3760	Phosphorylation	SSGLGSASSIQAAVR CCCCCCHHHHHHHHH	29.66	21082442
3761	Phosphorylation	SGLGSASSIQAAVRQ CCCCCHHHHHHHHHH	20.59	25521595
3794	Phosphorylation	RRQQQAATSESSNQS HHHHHHHHCCCCCCH	35.72	23684622
3795	Phosphorylation	RQQQAATSESSNQSE HHHHHHHCCCCCCHH	30.65	23684622
3810	Phosphorylation	TSVRREESPMDVDQP HCCCCCCCCCCCCCC	22.29	25521595
3818	Phosphorylation	PMDVDQPSPSAQDTQ CCCCCCCCCCCCCCC	26.34	25521595
3820	Phosphorylation	DVDQPSPSAQDTQSI CCCCCCCCCCCCCEE	42.79	25521595
3824	Phosphorylation	PSPSAQDTQSIVISD CCCCCCCCCEEEEEC	16.49	25619855
3826	Phosphorylation	PSAQDTQSIVISDGT CCCCCCCEEEEECCC	22.40	25619855
3830	Phosphorylation	DTQSIVISDGTPQGE CCCEEEEECCCCCCH	21.06	25619855
3833	Phosphorylation	SIVISDGTPQGEKEK EEEEECCCCCCHHHC	19.89	25619855
3909	Phosphorylation	PVRDTRESQLAHIKD CCCCCCHHHHHHCCC	27.48	25619855
3915	Ubiquitination	ESQLAHIKDEPPPLS HHHHHHCCCCCCCCC	46.29	-
3922	Phosphorylation	KDEPPPLSPAPLTPA CCCCCCCCCCCCCCC	25.45	26824392
3927	Phosphorylation	PLSPAPLTPATPSSL CCCCCCCCCCCCHHC	15.15	26824392
3930	Phosphorylation	PAPLTPATPSSLDPF CCCCCCCCCHHCCCC	25.55	22942356
3932	Phosphorylation	PLTPATPSSLDPFFS CCCCCCCHHCCCCCC	38.90	25619855
3933	Phosphorylation	LTPATPSSLDPFFSR CCCCCCHHCCCCCCC	37.69	25619855
3939	Phosphorylation	SSLDPFFSREPSSMH HHCCCCCCCCCCCCC	35.79	25619855
3976	Phosphorylation	LNQILRQSTTHLADG HHHHHHHCCCCCCCC	28.92	22871156
3977	Phosphorylation	NQILRQSTTHLADGP HHHHHHCCCCCCCCC	15.19	22871156
3978	Phosphorylation	QILRQSTTHLADGPF HHHHHCCCCCCCCCC	21.82	22871156
4000	Ubiquitination	RVLDFDVKRKYFRQE EECCHHHHHHHHHHH	45.04	-
4002	Ubiquitination	LDFDVKRKYFRQELE CCHHHHHHHHHHHHH	42.92	-
4017	Ubiquitination	RLDEGLRKEDMAVHV HHHHHCCHHHCCHHH	64.21	-
4081	Phosphorylation	REMFNPMYALFRTSP HHHHCCHHHHCCCCC	11.04	18563927
4137	Ubiquitination	YFTRSFYKHILGKSV HHHHHHHHHHHCCCE	23.35	-
4224	Ubiquitination	RMTGAIRKQLAAFLE HHHHHHHHHHHHHHH	43.48	-
4274	Phosphorylation	SNTEYHKYQSNSIQI CCCCEEECCCCCHHH	12.12	-
4298	Ubiquitination	FDQADRAKFLQFVTG CCHHHHHHHHHHHHC	47.72	-
4308	Ubiquitination	QFVTGTSKVPLQGFA HHHHCCCCCCCCHHH	47.94	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of HUWE1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of HUWE1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HUWE1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MCL1_MOUSE	Mcl1	physical	21613222
CDN2A_MOUSE	Cdkn2a	physical	22754359
ARF_MOUSE	Cdkn2a	physical	22754359
P53_MOUSE	Trp53	physical	22754359
MYC_MOUSE	Myc	physical	22754359
DP13A_MOUSE	Appl1	physical	28671696
ARHGC_MOUSE	Arhgef12	physical	28671696
ARHGI_MOUSE	Arhgef18	physical	28671696
BIN1_MOUSE	Bin1	physical	28671696
KCC2A_MOUSE	Camk2a	physical	28671696
KCC2B_MOUSE	Camk2b	physical	28671696
CALX_MOUSE	Canx	physical	28671696
CN37_MOUSE	Cnp	physical	28671696
DPYL1_MOUSE	Crmp1	physical	28671696
DPYL2_MOUSE	Dpysl2	physical	28671696
E41L3_MOUSE	Epb4.1l3	physical	28671696
RB6I2_MOUSE	Erc1	physical	28671696
F1712_MOUSE	Fam171a2	physical	28671696
GIT1_MOUSE	Git1	physical	28671696
GLNA_MOUSE	Glul	physical	28671696
HNRL2_MOUSE	Hnrnpul2	physical	28671696
HOOK3_MOUSE	Hook3	physical	28671696
KANK2_MOUSE	Kank2	physical	28671696
K0319_MOUSE	D130043K22Rik	physical	28671696
KI21A_MOUSE	Kif21a	physical	28671696
KIF5C_MOUSE	Kif5c	physical	28671696
NOS1_MOUSE	Nos1	physical	28671696
NPTN_MOUSE	Nptn	physical	28671696
NSF_MOUSE	Nsf	physical	28671696
OGT1_MOUSE	Ogt	physical	28671696
MBRL_MOUSE	Tmem259	physical	28671696
PDE4A_MOUSE	Pde4a	physical	28671696
KPCG_MOUSE	Prkcg	physical	28671696
PURA_MOUSE	Pura	physical	28671696
RS27A_MOUSE	Rps27a	physical	28671696
SOGA3_MOUSE	Soga3	physical	28671696
SPTB2_MOUSE	Sptbn1	physical	28671696
TNIK_MOUSE	Tnik	physical	28671696
TENR_MOUSE	Tnr	physical	28671696
TP53B_MOUSE	Trp53bp1	physical	28671696
TRIM3_MOUSE	Trim3	physical	28671696
WDR81_MOUSE	Wdr81	physical	28671696
WDR91_MOUSE	Wdr91	physical	28671696
XRCC5_MOUSE	Xrcc5	physical	28671696
XRCC6_MOUSE	Xrcc6	physical	28671696
EF2_MOUSE	Eef2	physical	28671696
NEST_MOUSE	Nes	physical	28671696

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry.";
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
Mol. Cell. Proteomics 8:904-912(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1907, AND MASSSPECTROMETRY.
PubMed: 19131326

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3758 AND SER-3810, ANDMASS SPECTROMETRY.
PubMed: 17242355

"Protein phosphorylation and expression profiling by Yin-yangmultidimensional liquid chromatography (Yin-yang MDLC) massspectrometry.";
Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.;
J. Proteome Res. 6:250-262(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1907, AND MASSSPECTROMETRY.
PubMed: 17203969

"Phosphoproteomic analysis of the developing mouse brain.";
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
Mol. Cell. Proteomics 3:1093-1101(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3758, AND MASSSPECTROMETRY.
PubMed: 15345747