MCL1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MCL1_MOUSE
• UniProt AC: P97287
• Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1 homolog
• Gene Name: Mcl1
• Organism: Mus musculus (Mouse).
• Sequence Length: 331
• Subcellular Localization: Membrane; Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.
• Protein Description: Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 2 has antiapoptotic activity..
• Protein Sequence: MFGLRRNAVIGLNLYCGGASLGAGGGSPAGARLVAEEAKARREGGGEAALLPGARVVARPPPVGAEDPDVTASAERRLHKSPGLLAVPPEEMAASAAAAIVSPEEELDGCEPEAIGKRPAVLPLLERVSEAAKSSGADGSLPSTPPPPEEEEDDLYRQSLEIISRYLREQATGSKDSKPLGEAGAAGRRALETLRRVGDGVQRNHETAFQGMLRKLDIKNEGDVKSFSRVMVHVFKDGVTNWGRIVTLISFGAFVAKHLKSVNQESFIEPLAETITDVLVRTKRDWLVKQRGWDGFVEFFHVQDLEGGIRNVLLAFAGVAGVGAGLAYLIR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
15	Phosphorylation	AVIGLNLYCGGASLG CEEEEEEEECCCCCC	6.49	26026062
20	Phosphorylation	NLYCGGASLGAGGGS EEEECCCCCCCCCCC	30.52	26026062
27	Phosphorylation	SLGAGGGSPAGARLV CCCCCCCCHHHHHHH	18.17	26643407
39	Ubiquitination	RLVAEEAKARREGGG HHHHHHHHHHHCCCC	45.78	-
71	Phosphorylation	GAEDPDVTASAERRL CCCCCCCCHHHHHHH	23.44	28066266
73	Phosphorylation	EDPDVTASAERRLHK CCCCCCHHHHHHHHC	22.69	28066266
81	Phosphorylation	AERRLHKSPGLLAVP HHHHHHCCCCCCCCC	17.29	22006019
102	Phosphorylation	SAAAAIVSPEEELDG HHHHHHCCCHHHHCC	22.08	22817900
133	Ubiquitination	ERVSEAAKSSGADGS HHHHHHHHHCCCCCC	52.80	-
134	Phosphorylation	RVSEAAKSSGADGSL HHHHHHHHCCCCCCC	29.31	25619855
135	Phosphorylation	VSEAAKSSGADGSLP HHHHHHHCCCCCCCC	36.98	25619855
140	Phosphorylation	KSSGADGSLPSTPPP HHCCCCCCCCCCCCC	37.34	22942356
143	Phosphorylation	GADGSLPSTPPPPEE CCCCCCCCCCCCCHH	59.86	23984901
144	Phosphorylation	ADGSLPSTPPPPEEE CCCCCCCCCCCCHHH	37.56	27087446
156	Phosphorylation	EEEEDDLYRQSLEII HHHCCHHHHHHHHHH	17.38	25619855
159	Phosphorylation	EDDLYRQSLEIISRY CCHHHHHHHHHHHHH	20.97	26745281
164	Phosphorylation	RQSLEIISRYLREQA HHHHHHHHHHHHHHH	22.12	26745281
175	Ubiquitination	REQATGSKDSKPLGE HHHHHCCCCCCCHHH	68.33	-
178	Ubiquitination	ATGSKDSKPLGEAGA HHCCCCCCCHHHCHH	55.16	-
219	Ubiquitination	MLRKLDIKNEGDVKS HHHHCCCCCCCCCCC	48.92	22790023
225	Ubiquitination	IKNEGDVKSFSRVMV CCCCCCCCCCEEEEE	50.99	27667366
260	Ubiquitination	AFVAKHLKSVNQESF HHHHHHHHCCCHHHH	52.67	-
289	Ubiquitination	TKRDWLVKQRGWDGF CCHHHHHHCCCCCCE	31.91	27667366

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
102	S	Phosphorylation	Kinase	MAPK10	P53779	GPS
102	S	Phosphorylation	Kinase	JNK3	Q61831	PSP
140	S	Phosphorylation	Kinase	GSK3-ALPHA	Q2NL51	Uniprot
140	S	Phosphorylation	Kinase	GSK3-BETA	Q9WV60	Uniprot
140	S	Phosphorylation	Kinase	GSK-FAMILY	-	GPS
144	T	Phosphorylation	Kinase	MAPK1	P28482	GPS
144	T	Phosphorylation	Kinase	MAPK3	P27361	GPS
144	T	Phosphorylation	Kinase	MAPK8	Q91Y86	GPS
144	T	Phosphorylation	Kinase	MAPK10	P53779	GPS
144	T	Phosphorylation	Kinase	JNK3	Q61831	PSP
144	T	Phosphorylation	Kinase	MTOR	Q9JLN9	PSP
144	T	Phosphorylation	Kinase	MAPK	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
140	S	Phosphorylation	Phosphorylated on Ser-140, by GSK3, in response to IL3/interleukin-3 withdrawal.	16543145
140	S	Phosphorylation	Phosphorylation at Ser-140 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity.	16543145
140	S	Phosphorylation	Ubiquitination is induced by phosphorylation at Ser-140 (By similarity).	-
140	S	ubiquitylation	Phosphorylation at Ser-140 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity.	16543145
140	S	ubiquitylation	Ubiquitination is induced by phosphorylation at Ser-140 (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MCL1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PIN1_MOUSE	Pin1	physical	17670986
RBX1_MOUSE	Rbx1	physical	21368834
CUL1_MOUSE	Cul1	physical	21368834
SKP1_MOUSE	Skp1a	physical	21368834
TRI17_MOUSE	Trim17	physical	22976837
APR_MOUSE	Pmaip1	physical	23733106

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.