dbPTM

NSF_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NSF_MOUSE
UniProt AC	P46460
Protein Name	Vesicle-fusing ATPase
Gene Name	Nsf
Organism	Mus musculus (Mouse).
Sequence Length	744
Subcellular Localization	Cytoplasm.
Protein Description	Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack GRIA2 leads to influence GRIA2 membrane cycling (By similarity)..
Protein Sequence	MAGRTMQAARCPTDELSLSNCAVVNEKDFQSGQHVMVRTSPNHKYIFTLRTHPSVVPGCIAFSLPQRKWAGLSIGQDIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALMREEGASPLDFD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
11	S-nitrosylation	RTMQAARCPTDELSL CCCCCCCCCCCCCCC	3.50	22588120
21	S-nitrosylation	DELSLSNCAVVNEKD CCCCCCCCEEECHHH	2.46	22588120
31	Phosphorylation	VNEKDFQSGQHVMVR ECHHHCCCCCEEEEE	40.18	29899451
59	S-palmitoylation	HPSVVPGCIAFSLPQ CCCCCCCEEEECCCC	1.33	28680068
94	Phosphorylation	KAKQCIGTMTIEIDF HHHHHEEEEEEEEEE	7.39	-
105	Acetylation	EIDFLQKKNIDSNPY EEEEHHHCCCCCCCC	46.29	23806337
105	Malonylation	EIDFLQKKNIDSNPY EEEEHHHCCCCCCCC	46.29	26320211
105	Ubiquitination	EIDFLQKKNIDSNPY EEEEHHHCCCCCCCC	46.29	27667366
109	Phosphorylation	LQKKNIDSNPYDTDK HHHCCCCCCCCCHHH	35.59	25521595
161	Ubiquitination	AMDPSILKGEPASGK HCCHHHHCCCCCCCC	60.60	22790023
192	Phosphorylation	FEKAENSSLNLIGKA HHHHCCCCCCCCEEC	32.62	19854140
198	Ubiquitination	SSLNLIGKAKTKENR CCCCCCEECCCHHHH	39.45	22790023
207	Phosphorylation	KTKENRQSIINPDWN CCHHHHHCCCCCCCC	23.38	25521595
217	Ubiquitination	NPDWNFEKMGIGGLD CCCCCHHHCCCCCCC	37.99	22790023
228	Phosphorylation	GGLDKEFSDIFRRAF CCCCHHHHHHHHHHH	30.78	22817900
250	S-nitrosocysteine	EIVEQMGCKHVKGIL HHHHHHCCCEEEEEE	2.01	-
250	S-nitrosylation	EIVEQMGCKHVKGIL HHHHHHCCCEEEEEE	2.01	16418269
259	Phosphorylation	HVKGILLYGPPGCGK EEEEEEEECCCCCCH	24.92	22817900
264	S-nitrosylation	LLYGPPGCGKTLLAR EEECCCCCCHHHHHH	6.61	16418269
264	S-nitrosocysteine	LLYGPPGCGKTLLAR EEECCCCCCHHHHHH	6.61	-
266	Ubiquitination	YGPPGCGKTLLARQI ECCCCCCHHHHHHHH	39.82	22790023
293	Ubiquitination	NGPEILNKYVGESEA CCHHHHHHHCCCCHH	37.56	22790023
294	Phosphorylation	GPEILNKYVGESEAN CHHHHHHHCCCCHHH	16.77	29899451
304	Ubiquitination	ESEANIRKLFADAEE CCHHHHHHHHCCHHH	44.13	22790023
339	Phosphorylation	AICKQRGSMAGSTGV HHHHHCCCCCCCCCC	13.98	20415495
343	Phosphorylation	QRGSMAGSTGVHDTV HCCCCCCCCCCHHHH	16.67	22817900
344	Phosphorylation	RGSMAGSTGVHDTVV CCCCCCCCCCHHHHH	42.16	29899451
349	Phosphorylation	GSTGVHDTVVNQLLS CCCCCHHHHHHHHHH	16.10	20415495
356	Phosphorylation	TVVNQLLSKIDGVEQ HHHHHHHHCCCCHHH	35.84	20415495
401	Ubiquitination	EIGLPDEKGRLQILH EECCCCCCCCEEEEE	57.63	22790023
411	Phosphorylation	LQILHIHTARMRGHQ EEEEEEECCHHCCCE	18.50	23970565
421	Phosphorylation	MRGHQLLSADVDIKE HCCCEEECCCCCHHH	30.89	22817900
434	Ubiquitination	KELAVETKNFSGAEL HHHHHCCCCCCHHHH	42.35	22790023
437	Phosphorylation	AVETKNFSGAELEGL HHCCCCCCHHHHHHH	46.62	22817900
450	Phosphorylation	GLVRAAQSTAMNRHI HHHHHHHHHHHHHHH	17.26	22817900
460	Phosphorylation	MNRHIKASTKVEVDM HHHHHCCCCEEEECH	24.61	22817900
461	Phosphorylation	NRHIKASTKVEVDME HHHHCCCCEEEECHH	43.92	22817900
462	Ubiquitination	RHIKASTKVEVDMEK HHHCCCCEEEECHHH	33.62	22790023
469	Ubiquitination	KVEVDMEKAESLQVT EEEECHHHHHHCCCC	51.31	22790023
472	Phosphorylation	VDMEKAESLQVTRGD ECHHHHHHCCCCCHH	29.03	29176673
531	Phosphorylation	LVQQTKNSDRTPLVS EEEECCCCCCCCEEE	29.99	20531401
534	Phosphorylation	QTKNSDRTPLVSVLL ECCCCCCCCEEEEEE	26.43	20531401
538	Phosphorylation	SDRTPLVSVLLEGPP CCCCCEEEEEECCCC	18.09	20531401
547	Phosphorylation	LLEGPPHSGKTALAA EECCCCCCCHHHHHH	48.00	20531401
550	Phosphorylation	GPPHSGKTALAAKIA CCCCCCHHHHHHHHH	30.35	20531401
555	Ubiquitination	GKTALAAKIAEESNF CHHHHHHHHHHHCCC	36.52	22790023
569	Phosphorylation	FPFIKICSPDKMIGF CCEEEECCCCCCCCC	39.34	22817900
577	Phosphorylation	PDKMIGFSETAKCQA CCCCCCCCHHHHHHH	28.91	25521595
599	S-nitrosylation	AYKSQLSCVVVDDIE HHHHCCCEEEECCHH	3.51	22588120
699	Ubiquitination	TTIAQQVKGKKVWIG CCHHHHHCCCEEEEH	61.75	22790023
702	Ubiquitination	AQQVKGKKVWIGIKK HHHHCCCEEEEHHHH	52.01	22790023
708	Methylation	KKVWIGIKKLLMLIE CEEEEHHHHHHHHHH	31.96	-
709	Methylation	KVWIGIKKLLMLIEM EEEEHHHHHHHHHHH	44.95	-
739	Phosphorylation	LMREEGASPLDFD-- HHHHCCCCCCCCC--	36.53	25521595

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
460	S	Phosphorylation	Kinase	PRKCE	P16054	GPS
569	S	Phosphorylation	Kinase	CDK16	Q00536	PSP
569	S	Phosphorylation	Kinase	CDK16	Q04735	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
569	S	Phosphorylation		16461345
Phosphorylation at Ser-569 interferes with homohexamerization.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NSF_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of NSF_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NSF_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-259, AND MASSSPECTROMETRY.	18034455 [Abstract]

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