ARF_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ARF_MOUSE
• UniProt AC: Q64364
• Protein Name: Tumor suppressor ARF {ECO:0000305}
• Gene Name: Cdkn2a {ECO:0000312|EMBL:AAB35770.1, ECO:0000312|MGI:MGI:104738}
• Organism: Mus musculus (Mouse).
• Sequence Length: 169
• Subcellular Localization: Nucleus, nucleolus . Nucleus, nucleoplasm.; Isoform smARF: Mitochondrion .
• Protein Description: Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination (By similarity). Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform smARF may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP..
• Protein Sequence: MGRRFLVTVRIQRAGRPLQERVFLVKFVRSRRPRTASCALAFVNMLLRLERILRRGPHRNPGPGDDDGQRSRSSSSAQLRCRFELRGPHYLLPPGARRSAGRLPGHAGGAARVRGSAGCARCLGSPAARLGPRAGTSRHRAIFAFRWVLFVFRWVVFVYRWERRPDRRA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
26	Ubiquitination	QERVFLVKFVRSRRP HHHEEEHHHHHCCCC		-
35	Phosphorylation	VRSRRPRTASCALAF HHCCCCCCHHHHHHH		26643407
37	Phosphorylation	SRRPRTASCALAFVN CCCCCCHHHHHHHHH		26643407
71	Phosphorylation	GDDDGQRSRSSSSAQ CCCCCCCCCCCCCCE		-
75	Phosphorylation	GQRSRSSSSAQLRCR CCCCCCCCCCEEEEE		29550500
76	Phosphorylation	QRSRSSSSAQLRCRF CCCCCCCCCEEEEEE		29550500
99	Phosphorylation	LPPGARRSAGRLPGH CCCCCCCCCCCCCCC		27149854
125	Phosphorylation	GCARCLGSPAARLGP HHHHHHCCHHHHHCC		25263469
159	Phosphorylation	FRWVVFVYRWERRPD HHHHHHHHHHHCCCC		20139300

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
71	S	Phosphorylation	Kinase	RPS6KB1	Q8BSK8	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ARF_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ARF_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TBRG1_MOUSE	Tbrg1	physical	17110379
SP1_MOUSE	Sp1	physical	21555589
MDM2_MOUSE	Mdm2	physical	10713175
RL9_MOUSE	Rpl9	physical	16582619
RS7_MOUSE	Rps7	physical	16582619
HS90B_MOUSE	Hsp90ab1	physical	16582619
ERF3A_MOUSE	Gspt1	physical	16582619
TBRG1_MOUSE	Tbrg1	physical	16582619
RABL6_MOUSE	Rabl6	physical	16582619
PEX19_MOUSE	Pex19	physical	16582619
NEB2_MOUSE	Ppp1r9b	physical	16582619
OSTP_MOUSE	Spp1	physical	16582619
ZFP91_MOUSE	Zfp91	physical	16582619
TBA1A_MOUSE	Tuba1a	physical	16582619
TPIS_MOUSE	Tpi1	physical	16582619
EIF3A_MOUSE	Eif3a	physical	16582619
PEX19_HUMAN	PEX19	physical	12581788
CARF_HUMAN	CDKN2AIP	physical	12581788
PEX19_HUMAN	PEX19	physical	12154087
NPM_MOUSE	Npm1	physical	15485902
MDM2_MOUSE	Mdm2	physical	15485902
MDM2_MOUSE	Mdm2	physical	23580573
MYC_MOUSE	Myc	physical	15361884
P53_MOUSE	Trp53	physical	9653180
CARF_HUMAN	CDKN2AIP	physical	12154087
CARF_MOUSE	Cdkn2aip	physical	12154087
ZN235_MOUSE	Zfp93	physical	16582619
MDM2_MOUSE	Mdm2	physical	22378045

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.