dbPTM

RB6I2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RB6I2_MOUSE
UniProt AC	Q99MI1
Protein Name	ELKS/Rab6-interacting/CAST family member 1
Gene Name	Erc1
Organism	Mus musculus (Mouse).
Sequence Length	1120
Subcellular Localization	Cytoplasm. Membrane Peripheral membrane protein. Golgi apparatus membrane Peripheral membrane protein. In neurons, localized closed to presynaptic membrane. Recruited on Golgi membranes by RAB6A in a GTP-dependent manner.
Protein Description	Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex (By similarity). May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport..
Protein Sequence	MYGSARSVGKVEPSSQSPGRSPRLPRSPRLGHRRTNSTGGSSGNSVGGGSGKTLSMENIQSLNAAYATSGPMYLSDHENVGAETPKSTMTLGRSGGRLPYGVRMTAMGSSPNIASSGVASDTIAFGEHHLPPVSMASTVPHSLRQARDNTIMDLQTQLKEVLRENDLLRKDVEVKESKLSSSMNSIKTFWSPELKKERALRKDEASKITIWKEQYRVVQEENQHMQMTIQALQDELRIQRDLNQLFQQDSSSRTGEPCVAELTEENFQRLHAEHERQAKELFLLRKTLEEMELRIETQKQTLNARDESIKKLLEMLQSKGLSAKATEEDHERTRRLAEAEMHVHHLESLLEQKEKENNMLREEMHRRFENAPDSAKTKALQTVIEMKDSKISSMERGLRDLEEEIQMLKSNGALSSEEREEEMKQMEVYRSHSKFMKNKVEQLKEELSSKDAQGEELKKRAAGLQSEIGQVKQELSRKDTELLALQTKLETLTNQFSDSKQHIEVLKESLTAKEQRAAILQTEVDALRLRLEEKETMLNKKTKQIQDMAEEKGTQAGEIHDLKDMLDVKERKVNVLQKKIENLQEQLRDKEKQMSSLKERVKSLQADTTNTDTALTTLEEALADKERTIERLKEQRDRDEREKQEEIDTYKKDLKDLREKVSLLQGDLSEKEASLLDIKEHASSLASSGLKKDSRLKTLEIALEQKKEECLKMESQLKKAHEATLEARASPEMSDRIQQLEREISRYKDESSKAQTEVDRLLEILKEVENEKNDKDKKIAELESLTSRQVKDQNKKVANLKHKEQVEKKKSAQMLEEARRREDSLSDSSQQLQDSLRKKDDRIEELEEALRESVQITAEREMVLAQEESARTNAEKQVEELLMAMEKVKQELESMKAKLSSTQQSLAEKETHLTNLRAERRKHLEEVLEMKQEALLAAISEKDANIALLELSSSKKKTQEEVAALKREKDRLVQQLKQQTQNRMKLMADNYEDDHFRSSRSNQTNHKPSPDQIIQPLLELDQNRSKLKLYIGHLTALCHDRDPLILRGLTPPASYNADGEQAAWENELQQMTQEQLQNELEKVEGDNAELQEFANTILQQIADHCPDILEQVVNALEESS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MYGSARSVG ------CCCCCCCCC	30.96	26643407
4	Phosphorylation	----MYGSARSVGKV ----CCCCCCCCCCC	21.44	23375375
7	Phosphorylation	-MYGSARSVGKVEPS -CCCCCCCCCCCCCC	34.63	27087446
10	Acetylation	GSARSVGKVEPSSQS CCCCCCCCCCCCCCC	41.07	23806337
14	Phosphorylation	SVGKVEPSSQSPGRS CCCCCCCCCCCCCCC	27.75	27742792
15	Phosphorylation	VGKVEPSSQSPGRSP CCCCCCCCCCCCCCC	45.45	25521595
17	Phosphorylation	KVEPSSQSPGRSPRL CCCCCCCCCCCCCCC	31.13	25521595
21	Phosphorylation	SSQSPGRSPRLPRSP CCCCCCCCCCCCCCC	21.93	25521595
27	Phosphorylation	RSPRLPRSPRLGHRR CCCCCCCCCCCCCCC	16.66	25338131
35	Phosphorylation	PRLGHRRTNSTGGSS CCCCCCCCCCCCCCC	33.21	27087446
37	Phosphorylation	LGHRRTNSTGGSSGN CCCCCCCCCCCCCCC	28.02	25521595
38	Phosphorylation	GHRRTNSTGGSSGNS CCCCCCCCCCCCCCC	47.86	27087446
41	Phosphorylation	RTNSTGGSSGNSVGG CCCCCCCCCCCCCCC	36.10	27087446
42	Phosphorylation	TNSTGGSSGNSVGGG CCCCCCCCCCCCCCC	45.31	27087446
45	Phosphorylation	TGGSSGNSVGGGSGK CCCCCCCCCCCCCCC	26.18	23684622
50	Phosphorylation	GNSVGGGSGKTLSME CCCCCCCCCCCCCHH	40.15	29899451
53	Phosphorylation	VGGGSGKTLSMENIQ CCCCCCCCCCHHHHH	27.82	26239621
55	Phosphorylation	GGSGKTLSMENIQSL CCCCCCCCHHHHHHH	29.31	21082442
61	Phosphorylation	LSMENIQSLNAAYAT CCHHHHHHHHHHHHC	21.57	26643407
66	Phosphorylation	IQSLNAAYATSGPMY HHHHHHHHHCCCCCC	14.17	26643407
68	Phosphorylation	SLNAAYATSGPMYLS HHHHHHHCCCCCCCC	22.88	26643407
69	Phosphorylation	LNAAYATSGPMYLSD HHHHHHCCCCCCCCC	32.50	26643407
73	Phosphorylation	YATSGPMYLSDHENV HHCCCCCCCCCCCCC	13.24	26643407
75	Phosphorylation	TSGPMYLSDHENVGA CCCCCCCCCCCCCCC	21.31	26643407
84	Phosphorylation	HENVGAETPKSTMTL CCCCCCCCCCCCEEC	35.17	26643407
87	Phosphorylation	VGAETPKSTMTLGRS CCCCCCCCCEECCCC	25.31	18779572
88	Phosphorylation	GAETPKSTMTLGRSG CCCCCCCCEECCCCC	21.94	18779572
90	Phosphorylation	ETPKSTMTLGRSGGR CCCCCCEECCCCCCC	26.58	18779572
94	Phosphorylation	STMTLGRSGGRLPYG CCEECCCCCCCCCCC	43.74	-
100	Phosphorylation	RSGGRLPYGVRMTAM CCCCCCCCCEEEEEC	32.61	29514104
105	Phosphorylation	LPYGVRMTAMGSSPN CCCCEEEEECCCCCC	11.70	23984901
109	Phosphorylation	VRMTAMGSSPNIASS EEEEECCCCCCCCCC	29.54	26824392
110	Phosphorylation	RMTAMGSSPNIASSG EEEECCCCCCCCCCC	19.08	23984901
115	Phosphorylation	GSSPNIASSGVASDT CCCCCCCCCCCCCCC	24.67	23984901
116	Phosphorylation	SSPNIASSGVASDTI CCCCCCCCCCCCCCE	28.41	26643407
120	Phosphorylation	IASSGVASDTIAFGE CCCCCCCCCCEEECC	32.64	23984901
122	Phosphorylation	SSGVASDTIAFGEHH CCCCCCCCEEECCCC	16.23	23984901
134	Phosphorylation	EHHLPPVSMASTVPH CCCCCCCCHHHCCCH	17.88	26643407
134	O-linked_Glycosylation	EHHLPPVSMASTVPH CCCCCCCCHHHCCCH	17.88	22517741
137	Phosphorylation	LPPVSMASTVPHSLR CCCCCHHHCCCHHHH	22.69	26643407
138	Phosphorylation	PPVSMASTVPHSLRQ CCCCHHHCCCHHHHH	28.63	26643407
150	Phosphorylation	LRQARDNTIMDLQTQ HHHHHHCCHHHHHHH	23.20	-
187	Acetylation	SSSMNSIKTFWSPEL HHHHHHHHHHCCHHH	37.17	6569611
188	Phosphorylation	SSMNSIKTFWSPELK HHHHHHHHHCCHHHH	28.77	25777480
191	Phosphorylation	NSIKTFWSPELKKER HHHHHHCCHHHHHHH	12.33	26824392
250	Phosphorylation	NQLFQQDSSSRTGEP HHHHHHCCCCCCCCC	26.35	25619855
251	Phosphorylation	QLFQQDSSSRTGEPC HHHHHCCCCCCCCCC	31.93	25619855
252	Phosphorylation	LFQQDSSSRTGEPCV HHHHCCCCCCCCCCE	37.50	27742792
374	Phosphorylation	RFENAPDSAKTKALQ HHHCCCCHHHHHHHH	30.52	26824392
415	Phosphorylation	LKSNGALSSEEREEE HHHCCCCCHHHHHHH	34.86	26643407
416	Phosphorylation	KSNGALSSEEREEEM HHCCCCCHHHHHHHH	44.31	26643407
491	Phosphorylation	ALQTKLETLTNQFSD HHHHHHHHHHHHCCC	49.68	24759943
572	Malonylation	MLDVKERKVNVLQKK HHCHHHHHHHHHHHH	38.97	26320211
592	Acetylation	EQLRDKEKQMSSLKE HHHHHHHHHHHHHHH	57.97	7744499
603	Phosphorylation	SLKERVKSLQADTTN HHHHHHHHHHCCCCC	23.90	25338131
608	Phosphorylation	VKSLQADTTNTDTAL HHHHHCCCCCHHHHH	25.59	25338131
616	Phosphorylation	TNTDTALTTLEEALA CCHHHHHHHHHHHHH	27.20	22807455
617	Phosphorylation	NTDTALTTLEEALAD CHHHHHHHHHHHHHC	32.78	21082442
679	Ubiquitination	EASLLDIKEHASSLA HHHHHHHHHHHHHHH	43.01	-
688	Phosphorylation	HASSLASSGLKKDSR HHHHHHHCCCCHHHH	42.40	25338131
730	Phosphorylation	ATLEARASPEMSDRI HHHHHHCCHHHHHHH	18.81	26824392
734	Phosphorylation	ARASPEMSDRIQQLE HHCCHHHHHHHHHHH	23.29	29899451
811	Phosphorylation	EQVEKKKSAQMLEEA HHHHHHHHHHHHHHH	32.39	25338131
824	Phosphorylation	EARRREDSLSDSSQQ HHHHHHHHCCHHHHH	25.64	26824392
824 (in isoform 2)	Phosphorylation	-	25.64	22807455
826 (in isoform 2)	Phosphorylation	-	39.12	22807455
826	Phosphorylation	RRREDSLSDSSQQLQ HHHHHHCCHHHHHHH	39.12	21183079
828 (in isoform 2)	Phosphorylation	-	27.03	22807455
828	Phosphorylation	REDSLSDSSQQLQDS HHHHCCHHHHHHHHH	27.03	25777480
829	Phosphorylation	EDSLSDSSQQLQDSL HHHCCHHHHHHHHHH	27.26	25777480
829 (in isoform 2)	Phosphorylation	-	27.26	22807455
872	Phosphorylation	AQEESARTNAEKQVE HHHHHHHCCHHHHHH	38.20	21659604
889	Ubiquitination	LMAMEKVKQELESMK HHHHHHHHHHHHHHH	49.47	-
898	Ubiquitination	ELESMKAKLSSTQQS HHHHHHHHHHHHHHH	43.30	-
952	Phosphorylation	NIALLELSSSKKKTQ HHHHHHHCCCCCCCH	24.16	18779572
960 (in isoform 2)	Phosphorylation	-	60.72	30372032
965 (in isoform 2)	Phosphorylation	-	3.76	25521595
1009	Phosphorylation	NQTNHKPSPDQIIQP CCCCCCCCHHHCHHH	46.01	-
1050	Phosphorylation	PLILRGLTPPASYNA CCHHCCCCCCCCCCC	30.01	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of RB6I2_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RB6I2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RB6I2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of RB6I2_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RB6I2_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252, AND MASSSPECTROMETRY.	17242355 [Abstract]