dbPTM

NEST_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NEST_MOUSE
UniProt AC	Q6P5H2
Protein Name	Nestin
Gene Name	Nes
Organism	Mus musculus (Mouse).
Sequence Length	1864
Subcellular Localization
Protein Description	Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division (By similarity). Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells..
Protein Sequence	MEGCVGEESFQMWELNRRLEAYLTRVKTLEEQNQLLSAELGGLRAQSGDASWRARADDELAALRVLVDQRWREKHEAEVQRDNLAEELESVAGRCQQVRLARERTIEEAACSRRALEAEKNARGWLSTQAAELERELEALRASHEEERAHLNAQAACTPRRPPAPAHASPIRAPEVEELARRLGEVWRGAVRDYQERVAHMESSLGQARERLGQAVRGARESRLEVQQLQADRDSLQERREALEQRLEGRWQDRLQATEKFQLAVEALEQEKQGLQSQIAQILEGGQQLAHLKMSLSLEVATYRTLLEAENSRLQTPGRSSQASLGFPDPKLKLHFLGIPEDQHLGSVLPVLSPTSFSSPLPNTLETPVTAFLKTQEFLKARTPTLASTPIPPMSEAPYPKNAEVRAQDVPHSLLQGGRQQAPEPLWAEATVPSSTGVLPELEEPGGEQPDHFPDDPTSLAPPLNPHHSILEAKDRESSESRVSSIFQEEEGQIWELVKKEAATEVKVENSLAQEIQESGLDTEEIQDSQGPLQMETLEALGDEPLMSLKTQNHETPGKENCNSSIEENSGTVKSPEKEKQTPLKSLEEKNVEAEKTLENGVLELSKPLGEEEPRMEDQELMSPEHTLETVSFLGKENQEVVRSSEEQNLESLITFKEESQYPLGGPEAEDQMLERLVEKEDQRFPRSPEEDQQAFRPLEKENQEPLRFEEAEDQVLERLIEKERQESLKSPEEEDQQAFRLLEKENQEPLRFEDAEDQVLERLIEKERQESLKSPEEEDQQAFRLLEKENQEPLRFEEAEDQVLERLVEKESQESLKSPEEEDQRTGKPLEKENQESLRSLDENQETIVLLESKNQRPLRSLEVEEEEQRIVKPLEKVSQVSLESLEKENVQSPRYLEEDDHMIKSLLEDKTHEILGSLEDRNGENFIPPENETQGSLRPPEEEDQRIVNHLEKESQEFLRSPEAEEEEEQVMVRSLEGENHDPLSSVVKEEQMAESKLENESQDSRKSLEDESQETFGSLEKENLESLRSLAGQDQEEQKLEQETQQPLRAVEDEQMTVNPPEKVDPELPKPLRNDQEVVRSLDKENQESLVSLNEGGMETVKSSETENIESLETVGECLGRRKSVDTQEPLWSTEVTSETIEPLEDETQEPLGCVDENQEVLTPLERESQELRSLGKWNPETVESPGGVEDSQQCLEVEEGPEREQHQESLRSLGEVEWELPGSGSQQRWEDVVEDGEGQEASLGATGVETEDKAELHLRGQGGEEKAVEEGELLQDAVGEAWSLGSSEPKEQRVPAEPLDDLEGQPEQTGTLEVPVAQGMPEATEQDEDRAQAGEQDSVEVTLGLEAARAGLELEQEVVGLEDPRHFAREEAIHPSLGEESVKAKIDQGLEEPGKEPKEAGALDSGIPELPKTSSETLECKGWEESGEGWGEEEASLETSDHEGSHAPQPRPPKTEEDEGLQAALTVPGPKLLEPCSPIPILTDAHELQPQAEGIQEAGWQPEAGTEALGRVEDEPEFGRGEIPEGLQDWEEGREDSEADELGETLPDSTPLGLYLKSPASPKWEQAGEQRLFPQGEARKEGWSPAALAAQGLSDPPEEEQQGHDSDLSSEEFEDLGTEASLLPGVPKEVSDHLGQEPPVLQPACWDQGGESDGFADEEESGEEGEEEDADEEEGAESGTQWWGPGPSGGGVKVQDVTQRGDLEHESVGDSGLWDDGLSGAAANVLVTALETVSQDSAEPSGSEGSESASLEGEEGQAIDHLDAPQEVTSVVPGAGDTFDISGQGPNLESEQVNGRMENGLEQAEGQVVLHGDEDQGIPLQEQGTLKAPLVGSPVHLGPSQPLKFTLSGVDGDSWSSGED

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEGCVGEE -------CCCCCCHH	43.29	-
47	Phosphorylation	LGGLRAQSGDASWRA HCCHHCCCCCCCHHH	37.34	24899341
90	Phosphorylation	NLAEELESVAGRCQQ CHHHHHHHHHHHHHH	29.86	26370283
105	Phosphorylation	VRLARERTIEEAACS HHHHHHHHHHHHHHH	28.17	-
143	Phosphorylation	ELEALRASHEEERAH HHHHHHHCHHHHHHH	25.62	24899341
158	Phosphorylation	LNAQAACTPRRPPAP HHHCCCCCCCCCCCC	18.33	25263469
169	Phosphorylation	PPAPAHASPIRAPEV CCCCCCCCCCCCHHH	15.82	26824392
235	Phosphorylation	QLQADRDSLQERREA HHHHCHHHHHHHHHH	32.61	26370283
297	Phosphorylation	AHLKMSLSLEVATYR HHHHHHHHHHHHHHH	18.38	26824392
312	Phosphorylation	TLLEAENSRLQTPGR HHHHHHHHCCCCCCC	26.35	27149854
316	Phosphorylation	AENSRLQTPGRSSQA HHHHCCCCCCCCCCC	32.10	23384938
320	Phosphorylation	RLQTPGRSSQASLGF CCCCCCCCCCCCCCC	32.49	26370283
321	Phosphorylation	LQTPGRSSQASLGFP CCCCCCCCCCCCCCC	28.53	25168779
324	Phosphorylation	PGRSSQASLGFPDPK CCCCCCCCCCCCCCC	22.76	26370283
353	Phosphorylation	GSVLPVLSPTSFSSP CCCCCCCCCCCCCCC	26.77	26824392
355	Phosphorylation	VLPVLSPTSFSSPLP CCCCCCCCCCCCCCC	38.74	29109428
356	Phosphorylation	LPVLSPTSFSSPLPN CCCCCCCCCCCCCCC	26.90	28059163
359	Phosphorylation	LSPTSFSSPLPNTLE CCCCCCCCCCCCCCC	28.57	-
367	Phosphorylation	PLPNTLETPVTAFLK CCCCCCCCCCHHHHH	26.60	-
383	Phosphorylation	QEFLKARTPTLASTP HHHHHHCCCCCCCCC	26.03	26824392
385	Phosphorylation	FLKARTPTLASTPIP HHHHCCCCCCCCCCC	34.05	-
388	Phosphorylation	ARTPTLASTPIPPMS HCCCCCCCCCCCCCC	37.52	27357545
389	Phosphorylation	RTPTLASTPIPPMSE CCCCCCCCCCCCCCC	21.45	26370283
399	Phosphorylation	PPMSEAPYPKNAEVR CCCCCCCCCCCCCCC	33.61	-
413	Phosphorylation	RAQDVPHSLLQGGRQ CHHCCCHHHHCCCCC	25.38	28066266
478	Phosphorylation	LEAKDRESSESRVSS HHCCCCCCCHHHHHH	40.06	21659604
479	Phosphorylation	EAKDRESSESRVSSI HCCCCCCCHHHHHHH	34.53	21659604
484	Phosphorylation	ESSESRVSSIFQEEE CCCHHHHHHHHHHHC	19.33	21659604
485	Phosphorylation	SSESRVSSIFQEEEG CCHHHHHHHHHHHCC	25.27	21659604
551	Phosphorylation	EPLMSLKTQNHETPG CCCCCCCCCCCCCCC	39.64	30635358
556	Phosphorylation	LKTQNHETPGKENCN CCCCCCCCCCCCCCC	29.77	30635358
564	Phosphorylation	PGKENCNSSIEENSG CCCCCCCCCCCCCCC	34.59	26824392
565	Phosphorylation	GKENCNSSIEENSGT CCCCCCCCCCCCCCC	21.74	27087446
570	Phosphorylation	NSSIEENSGTVKSPE CCCCCCCCCCCCCCH	38.84	25168779
572	Phosphorylation	SIEENSGTVKSPEKE CCCCCCCCCCCCHHH	25.28	29550500
575	Phosphorylation	ENSGTVKSPEKEKQT CCCCCCCCCHHHCCC	33.67	27087446
585	Acetylation	KEKQTPLKSLEEKNV HHCCCCCCCHHHHCC	54.95	7712751
590	Acetylation	PLKSLEEKNVEAEKT CCCCHHHHCCHHHHH	57.84	7712759
596	Acetylation	EKNVEAEKTLENGVL HHCCHHHHHHHHCCE	66.51	7712773
597	Phosphorylation	KNVEAEKTLENGVLE HCCHHHHHHHHCCEE	30.70	26370283
606	Phosphorylation	ENGVLELSKPLGEEE HHCCEEECCCCCCCC	23.89	26370283
623	Phosphorylation	MEDQELMSPEHTLET CCCCCCCCHHHHHHH	39.50	27087446
627	Phosphorylation	ELMSPEHTLETVSFL CCCCHHHHHHHHHHC	25.32	27087446
630	Phosphorylation	SPEHTLETVSFLGKE CHHHHHHHHHHCCHH	25.27	28066266
632	Phosphorylation	EHTLETVSFLGKENQ HHHHHHHHHCCHHCH	23.36	28066266
644	Phosphorylation	ENQEVVRSSEEQNLE HCHHHHHCCHHCCHH	30.41	28418008
688	Phosphorylation	EDQRFPRSPEEDQQA HHHCCCCCHHHHHHC	36.58	27087446
728	Phosphorylation	IEKERQESLKSPEEE HHHHHHHHHCCCHHH	32.53	27087446
731	Phosphorylation	ERQESLKSPEEEDQQ HHHHHHCCCHHHHHH	42.72	27087446
772	Phosphorylation	IEKERQESLKSPEEE HHHHHHHHHCCCHHH	32.53	27087446
775	Phosphorylation	ERQESLKSPEEEDQQ HHHHHHCCCHHHHHH	42.72	27087446
813	Phosphorylation	ERLVEKESQESLKSP HHHHHHHHHHHHCCH	50.76	21659605
816	Phosphorylation	VEKESQESLKSPEEE HHHHHHHHHCCHHHH	33.20	21659605
819	Phosphorylation	ESQESLKSPEEEDQR HHHHHHCCHHHHHHH	42.72	26824392
838	Phosphorylation	LEKENQESLRSLDEN CHHHCHHHHHCCCCC	21.22	26824392
841	Phosphorylation	ENQESLRSLDENQET HCHHHHHCCCCCCCE	45.53	26824392
848	Phosphorylation	SLDENQETIVLLESK CCCCCCCEEEEEECC	13.46	30635358
854	Phosphorylation	ETIVLLESKNQRPLR CEEEEEECCCCCCCC	36.55	28494245
862	Phosphorylation	KNQRPLRSLEVEEEE CCCCCCCCCCCHHHH	36.24	26824392
880	Phosphorylation	VKPLEKVSQVSLESL CCCHHHHHHCCHHHH	35.61	24899341
883	Phosphorylation	LEKVSQVSLESLEKE HHHHHHCCHHHHHHC	20.84	27087446
886	Phosphorylation	VSQVSLESLEKENVQ HHHCCHHHHHHCCCC	47.31	27087446
894	Phosphorylation	LEKENVQSPRYLEED HHHCCCCCCCHHCCC	13.68	27087446
907	Phosphorylation	EDDHMIKSLLEDKTH CCHHHHHHHHCCCCH	27.54	23737553
919	Phosphorylation	KTHEILGSLEDRNGE CCHHHHHCCCCCCCC	25.74	28418008
957	Phosphorylation	VNHLEKESQEFLRSP HHHHHHHHHHHHHCC	46.18	24068923
963	Phosphorylation	ESQEFLRSPEAEEEE HHHHHHHCCCHHHHH	29.28	26824392
977	Phosphorylation	EEQVMVRSLEGENHD HHHHHHHHCCCCCCC	21.25	26824392
987	Phosphorylation	GENHDPLSSVVKEEQ CCCCCCHHHHHCHHH	26.86	29119230
1010	Phosphorylation	ESQDSRKSLEDESQE CCHHHHHHHHHHHHH	35.56	25521595
1015	Phosphorylation	RKSLEDESQETFGSL HHHHHHHHHHHHHHH	45.71	25521595
1018	Phosphorylation	LEDESQETFGSLEKE HHHHHHHHHHHHHHH	26.17	24899341
1021	Phosphorylation	ESQETFGSLEKENLE HHHHHHHHHHHHCHH	29.21	27087446
1029	Phosphorylation	LEKENLESLRSLAGQ HHHHCHHHHHHHCCC	32.05	24899341
1032	Phosphorylation	ENLESLRSLAGQDQE HCHHHHHHHCCCCHH	28.06	26370283
1084	Phosphorylation	NDQEVVRSLDKENQE CCHHHHHHCCCCCHH	29.34	26824392
1092	Phosphorylation	LDKENQESLVSLNEG CCCCCHHHCEECCCC	25.06	24899341
1095	Phosphorylation	ENQESLVSLNEGGME CCHHHCEECCCCCCC	30.67	26370283
1106	Phosphorylation	GGMETVKSSETENIE CCCCCCCCCCCCCCH	29.13	29119230
1107	Phosphorylation	GMETVKSSETENIES CCCCCCCCCCCCCHH	42.70	29119230
1109	Phosphorylation	ETVKSSETENIESLE CCCCCCCCCCCHHHH	35.96	22668510
1114	Phosphorylation	SETENIESLETVGEC CCCCCCHHHHHHHHH	27.92	22668510
1117	Phosphorylation	ENIESLETVGECLGR CCCHHHHHHHHHHCC	38.82	28066266
1127	Phosphorylation	ECLGRRKSVDTQEPL HHHCCCCCCCCCCCC	24.34	22817900
1130	Phosphorylation	GRRKSVDTQEPLWST CCCCCCCCCCCCEEE	32.45	29899451
1166	Phosphorylation	DENQEVLTPLERESQ CCCCCEECHHHHHHH	30.88	21659604
1177	Phosphorylation	RESQELRSLGKWNPE HHHHHHHHCCCCCCC	54.84	26824392
1185	Phosphorylation	LGKWNPETVESPGGV CCCCCCCCCCCCCCC	30.72	26824392
1188	Phosphorylation	WNPETVESPGGVEDS CCCCCCCCCCCCCCH	25.22	21659605
1195	Phosphorylation	SPGGVEDSQQCLEVE CCCCCCCHHHHEECC	14.51	-
1213	Phosphorylation	EREQHQESLRSLGEV CHHHHHHHHHHHCEE	23.63	24899341
1216	Phosphorylation	QHQESLRSLGEVEWE HHHHHHHHHCEEEEE	46.12	27742792
1227	Phosphorylation	VEWELPGSGSQQRWE EEEECCCCCCHHHHH	33.07	24899341
1229	Phosphorylation	WELPGSGSQQRWEDV EECCCCCCHHHHHHH	25.49	24899341
1246	Phosphorylation	DGEGQEASLGATGVE CCCCCEEECCCCCCC	26.95	26370283
1287	Phosphorylation	DAVGEAWSLGSSEPK HHHHHHHHCCCCCCH	29.87	26824392
1290	Phosphorylation	GEAWSLGSSEPKEQR HHHHHCCCCCCHHHC	36.50	28418008
1291	Phosphorylation	EAWSLGSSEPKEQRV HHHHCCCCCCHHHCC	57.00	29119230
1342	Phosphorylation	AQAGEQDSVEVTLGL HHHCCCCCEEHHHHH	21.63	21659604
1380	Phosphorylation	REEAIHPSLGEESVK HHHHCCCCCCHHHHH	34.54	26824392
1385	Phosphorylation	HPSLGEESVKAKIDQ CCCCCHHHHHHHHHC	25.31	30635358
1418	Phosphorylation	IPELPKTSSETLECK CCCCCCCCCCCEEEC	32.10	24899341
1419	Phosphorylation	PELPKTSSETLECKG CCCCCCCCCCEEECC	39.57	27149854
1443	Phosphorylation	EEEASLETSDHEGSH CCCCCCCCCCCCCCC	44.76	28418008
1444	Phosphorylation	EEASLETSDHEGSHA CCCCCCCCCCCCCCC	27.82	28418008
1481	Phosphorylation	PKLLEPCSPIPILTD CCCCCCCCCCCCCCC	36.26	21659605
1487	Phosphorylation	CSPIPILTDAHELQP CCCCCCCCCHHHCCC	31.68	25168779
1541	Phosphorylation	WEEGREDSEADELGE HHHCCCCCHHHHHHC	29.90	26824392
1549	Phosphorylation	EADELGETLPDSTPL HHHHHHCCCCCCCCC	41.62	29119230
1553	Phosphorylation	LGETLPDSTPLGLYL HHCCCCCCCCCCEEE	29.95	29119230
1554	Phosphorylation	GETLPDSTPLGLYLK HCCCCCCCCCCEEEC	29.88	-
1562	Phosphorylation	PLGLYLKSPASPKWE CCCEEECCCCCCHHH	24.09	27149854
1565	Phosphorylation	LYLKSPASPKWEQAG EEECCCCCCHHHHHC	30.32	26824392
1656	Phosphorylation	CWDQGGESDGFADEE HHCCCCCCCCCCCHH	46.43	-
1665	Phosphorylation	GFADEEESGEEGEEE CCCCHHHCCCCCCCC	55.58	-
1745	Phosphorylation	SQDSAEPSGSEGSES CCCCCCCCCCCCCCC	46.59	-
1747	Phosphorylation	DSAEPSGSEGSESAS CCCCCCCCCCCCCCC	43.02	-
1837	Phosphorylation	LKAPLVGSPVHLGPS CCCCCCCCCCCCCCC	19.10	27087446
1844	Phosphorylation	SPVHLGPSQPLKFTL CCCCCCCCCCCEEEE	42.50	24068923
1850	Phosphorylation	PSQPLKFTLSGVDGD CCCCCEEEEECCCCC	20.58	23737553
1852	Phosphorylation	QPLKFTLSGVDGDSW CCCEEEEECCCCCCC	33.44	23737553
1858	Phosphorylation	LSGVDGDSWSSGED- EECCCCCCCCCCCC-	34.09	23737553
1860	Phosphorylation	GVDGDSWSSGED--- CCCCCCCCCCCC---	32.20	27149854
1861	Phosphorylation	VDGDSWSSGED---- CCCCCCCCCCC----	39.69	27149854

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
919	S	Phosphorylation	Kinase	MTOR	Q9JLN9	PSP
1554	T	Phosphorylation	Kinase	CDK5	P49615	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NEST_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NEST_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of NEST_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NEST_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-688, AND MASSSPECTROMETRY.	19367708 [Abstract]
"Phosphoproteomic analysis of the developing mouse brain."; Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.; Mol. Cell. Proteomics 3:1093-1101(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1541, AND MASSSPECTROMETRY.	15345747 [Abstract]