NOS1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NOS1_MOUSE
• UniProt AC: Q9Z0J4
• Protein Name: Nitric oxide synthase, brain
• Gene Name: Nos1
• Organism: Mus musculus (Mouse).
• Sequence Length: 1429
• Subcellular Localization: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enri
• Protein Description: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Isoform NNOS Mu may be an effector enzyme for the dystrophin complex..
• Protein Sequence: MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
24	Ubiquitination	SVRLFKRKVGGLGFL EEEEEEEECCCEEEE	45.91	-
33	Ubiquitination	GGLGFLVKERVSKPP CCEEEEEECCCCCCC	41.78	-
89	Phosphorylation	EVLRGIASETHVVLI HHHHHHHCCCEEEEE	40.90	25293948
91	Phosphorylation	LRGIASETHVVLILR HHHHHCCCEEEEEEE	19.92	25293948
116	Phosphorylation	TTFTGDGTPKTIRVT EEECCCCCCCEEEEE	26.76	29899451
128	Phosphorylation	RVTQPLGTPTKAVDL EEEECCCCCCEEEEC	35.15	25521595
130	Phosphorylation	TQPLGTPTKAVDLSR EECCCCCCEEEECCC	31.32	22324799
131	Ubiquitination	QPLGTPTKAVDLSRQ ECCCCCCEEEECCCC	47.84	-
136	Phosphorylation	PTKAVDLSRQPSASK CCEEEECCCCCCCCC	25.14	22324799
140	Phosphorylation	VDLSRQPSASKDQPL EECCCCCCCCCCCCC	36.51	29899451
142	Phosphorylation	LSRQPSASKDQPLAV CCCCCCCCCCCCCCC	40.89	22324799
143	Ubiquitination	SRQPSASKDQPLAVD CCCCCCCCCCCCCCC	61.16	27667366
188	Ubiquitination	DPTMKNTKANLQDSG CCCCCCCCHHCCCCC	44.67	-
194	Phosphorylation	TKANLQDSGEQDELL CCHHCCCCCHHHHHH	31.13	25521595
216	Ubiquitination	SILTGGGKAVNRGGP HHHCCCCCCCCCCCC	53.05	-
225	Ubiquitination	VNRGGPAKAEMKDTG CCCCCCCCCCHHCCC	48.27	-
229	Ubiquitination	GPAKAEMKDTGIQVD CCCCCCHHCCCCCCC	43.58	-
245	Ubiquitination	DLDGKLHKAPPLGGE CCCCCCCCCCCCCCC	73.38	-
274	Phosphorylation	PVVLNNPYSENEQSP CEEECCCCCCCCCCC	30.64	25619855
275	Phosphorylation	VVLNNPYSENEQSPA EEECCCCCCCCCCCC	34.89	25619855
280	Phosphorylation	PYSENEQSPASGKQS CCCCCCCCCCCCCCC	19.09	25619855
283	Phosphorylation	ENEQSPASGKQSPTK CCCCCCCCCCCCCCC	50.32	25619855
287	Phosphorylation	SPASGKQSPTKNGSP CCCCCCCCCCCCCCC	37.71	25521595
289	Phosphorylation	ASGKQSPTKNGSPSR CCCCCCCCCCCCCCC	42.25	25619855
293	Phosphorylation	QSPTKNGSPSRCPRF CCCCCCCCCCCCCCE	29.15	29899451
370	Ubiquitination	DQYYSSIKRFGSKAH HHHHHHHHHHCCHHH	43.55	-
386	Ubiquitination	DRLEEVNKEIESTST HHHHHHHHHHHCCCE	66.30	-
739	Ubiquitination	KKLAEAVKFSAKLMG HHHHHHHHHHHHHHH	40.42	-
743	Ubiquitination	EAVKFSAKLMGQAMA HHHHHHHHHHHHHHH	37.50	-
759	Phosphorylation	RVKATILYATETGKS HHHEEEEEECCCCCC	13.97	-
761	Phosphorylation	KATILYATETGKSQA HEEEEEECCCCCCHH	22.11	-
763	Phosphorylation	TILYATETGKSQAYA EEEEECCCCCCHHHH	45.62	-
847	Phosphorylation	SYKVRFNSVSSYSDS HCEEEECCCCCCCCC	22.21	18096606
849	Phosphorylation	KVRFNSVSSYSDSRK EEEECCCCCCCCCCC	24.31	20415495
850	Phosphorylation	VRFNSVSSYSDSRKS EEECCCCCCCCCCCC	26.88	23375375
851	Phosphorylation	RFNSVSSYSDSRKSS EECCCCCCCCCCCCC	14.70	28576409
852	Phosphorylation	FNSVSSYSDSRKSSG ECCCCCCCCCCCCCC	30.93	20415495
854	Phosphorylation	SVSSYSDSRKSSGDG CCCCCCCCCCCCCCC	35.35	19060867
857	Phosphorylation	SYSDSRKSSGDGPDL CCCCCCCCCCCCCCH	38.14	25521595
858	Phosphorylation	YSDSRKSSGDGPDLR CCCCCCCCCCCCCHH	43.89	7686743
881 (in isoform 5)	Phosphorylation	-	3.97	28464351
956	Phosphorylation	NIEKANNSLISNDRS CHHHHHCCCCCCCCC	27.21	28464351
1199	Phosphorylation	VAIVSYHTRDGEGPV EEEEEEECCCCCCCC	23.40	28285833
1371	Ubiquitination	RIMTQQGKLSEEDAG HHHHHCCCCCHHHHH	44.49	-
1410	Phosphorylation	EVTNRLRSESIAFIE HHHHHHHHCEEEEEH	40.04	22324799
1412	Phosphorylation	TNRLRSESIAFIEES HHHHHHCEEEEEHHH	22.06	25521595
1419	Phosphorylation	SIAFIEESKKDTDEV EEEEEHHHHCCHHHH	32.01	29899451
1428	Phosphorylation	KDTDEVFSS------ CCHHHHCCC------	40.07	25521595
1446 (in isoform 5)	Phosphorylation	-		-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	Stub1	Q9WUD1	PMID:22199232

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NOS1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NOS1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DMD_MOUSE	Dmd	physical	7545544

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.