CDC20_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CDC20_MOUSE
• UniProt AC: Q9JJ66
• Protein Name: Cell division cycle protein 20 homolog
• Gene Name: Cdc20
• Organism: Mus musculus (Mouse).
• Sequence Length: 499
• Subcellular Localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
• Protein Description: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation..
• Protein Sequence: MAQFVFESDLHSLLQLDAPIPNAPVARWQRKAKEATGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRFIPQRSASQMEVASFLLSKENQPEDRGTPTKKEHQKAWSLNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWNAGSGDILQLLQMEQPGDYISSVAWIKEGNYLAVGTSNAEVQLWDVQQQKRLRNMTSHSARVSSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNIVNVWPSGPGESGWAPLQTFTQHQGAVKAVAWCPWQSNILATGGGTSDRHIRIWNVCSGACLSAVDVHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTARVLGLTMSPDGATVASAAADETLRLWRCFEMDPALRREREKASVAKSSLIHQGIR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
36	Phosphorylation	QRKAKEATGPAPSPM HHHHHHCCCCCCCHH	44.80	25266776
41	Phosphorylation	EATGPAPSPMRAANR HCCCCCCCHHHHHHH	33.17	26824392
51	Phosphorylation	RAANRSHSAGRTPGR HHHHHCCCCCCCCCC	33.39	25266776
55	Phosphorylation	RSHSAGRTPGRTPGK HCCCCCCCCCCCCCC	29.22	26824392
59	Phosphorylation	AGRTPGRTPGKSSSK CCCCCCCCCCCCCCC	42.16	26824392
66	Acetylation	TPGKSSSKVQTTPSK CCCCCCCCCCCCCCC	40.07	-
69	Phosphorylation	KSSSKVQTTPSKPGG CCCCCCCCCCCCCCC	43.60	25266776
70	Phosphorylation	SSSKVQTTPSKPGGD CCCCCCCCCCCCCCC	14.42	26824392
72	Phosphorylation	SKVQTTPSKPGGDRF CCCCCCCCCCCCCCC	49.76	25266776
84	Phosphorylation	DRFIPQRSASQMEVA CCCCCCCCHHHHHHH	27.33	26643407
86	Phosphorylation	FIPQRSASQMEVASF CCCCCCHHHHHHHHH	31.16	26239621
92	Phosphorylation	ASQMEVASFLLSKEN HHHHHHHHHHHCCCC	22.92	25777480
96	Phosphorylation	EVASFLLSKENQPED HHHHHHHCCCCCCCC	39.82	25777480
106	Phosphorylation	NQPEDRGTPTKKEHQ CCCCCCCCCCHHHHH	28.78	25263469
108	Phosphorylation	PEDRGTPTKKEHQKA CCCCCCCCHHHHHHH	55.37	25266776
134	Phosphorylation	EAKILRLSGKPQNAP HHHHEEECCCCCCCC	38.03	29514104
136	Ubiquitination	KILRLSGKPQNAPEG HHEEECCCCCCCCCH	39.80	-
144	Phosphorylation	PQNAPEGYQNRLKVL CCCCCCHHHHHHHHH	10.84	29514104
153	Phosphorylation	NRLKVLYSQKATPGS HHHHHHHCCCCCCCC	22.50	29514104
157	Phosphorylation	VLYSQKATPGSSRKT HHHCCCCCCCCCCCC	34.91	29514104
160	Phosphorylation	SQKATPGSSRKTCRY CCCCCCCCCCCCCCC	28.33	29514104
161	Phosphorylation	QKATPGSSRKTCRYI CCCCCCCCCCCCCCC	43.65	-
452	Phosphorylation	RVLGLTMSPDGATVA EEEEEEECCCCCHHH	18.01	22067460
490	Ubiquitination	REKASVAKSSLIHQG HHHHHHHHHHHHHCC	38.12	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	Fzr1	Q9R1K5	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
41	S	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
66	K	Acetylation	Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C).	22014574
72	S	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
92	S	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
153	S	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
157	T	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
161	S	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
485	K	ubiquitylation	Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex (By similarity).	-
490	K	ubiquitylation	Ubiquitinated at Lys-490 during prometaphase.	-
490	K	ubiquitylation	Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CDC20_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
APC16_HUMAN	ANAPC16	physical	20360068
CDC16_HUMAN	CDC16	physical	20360068
CDC20_HUMAN	CDC20	physical	20360068
APC10_HUMAN	ANAPC10	physical	20360068
APC7_HUMAN	ANAPC7	physical	20360068
KNL1_HUMAN	CASC5	physical	20360068
CDC26_HUMAN	CDC26	physical	20360068
BUB1_HUMAN	BUB1	physical	20360068
APC13_HUMAN	ANAPC13	physical	20360068
NUDC_HUMAN	NUDC	physical	20360068
ANC2_HUMAN	ANAPC2	physical	20360068
APC5_HUMAN	ANAPC5	physical	20360068
APC1_HUMAN	ANAPC1	physical	20360068
CDC23_HUMAN	CDC23	physical	20360068
MD2L1_HUMAN	MAD2L1	physical	20360068
APC4_HUMAN	ANAPC4	physical	20360068
BUB1B_HUMAN	BUB1B	physical	20360068
CDC27_HUMAN	CDC27	physical	20360068
CDC27_MOUSE	Cdc27	physical	22084091
ANC2_MOUSE	Anapc2	physical	22084091
FBX5_MOUSE	Fbxo5	physical	22084091
APC7_MOUSE	Anapc7	physical	22084091
SIR2_MOUSE	Sirt2	physical	22014574
CDC27_MOUSE	Cdc27	physical	22014574
FEZ1_MOUSE	Fez1	physical	24726361
BUB1B_MOUSE	Bub1b	physical	24726361
BUB1_HUMAN	BUB1	physical	26496610
BUB1B_HUMAN	BUB1B	physical	26496610
FMNL1_HUMAN	FMNL1	physical	26496610
CCNA2_HUMAN	CCNA2	physical	26496610
TCPZ_HUMAN	CCT6A	physical	26496610
CDC27_HUMAN	CDC27	physical	26496610
GLGB_HUMAN	GBE1	physical	26496610
IREB2_HUMAN	IREB2	physical	26496610
MD2L1_HUMAN	MAD2L1	physical	26496610
MARK1_HUMAN	MARK1	physical	26496610
STX3_HUMAN	STX3	physical	26496610
TCPG_HUMAN	CCT3	physical	26496610
CDC23_HUMAN	CDC23	physical	26496610
CDC16_HUMAN	CDC16	physical	26496610
BUB3_HUMAN	BUB3	physical	26496610
TCPH_HUMAN	CCT7	physical	26496610
TCPD_HUMAN	CCT4	physical	26496610
TCPB_HUMAN	CCT2	physical	26496610
TCPQ_HUMAN	CCT8	physical	26496610
TCPE_HUMAN	CCT5	physical	26496610
PALLD_HUMAN	PALLD	physical	26496610
APC13_HUMAN	ANAPC13	physical	26496610
ANC2_HUMAN	ANAPC2	physical	26496610
APC5_HUMAN	ANAPC5	physical	26496610
APC7_HUMAN	ANAPC7	physical	26496610
SAMD9_HUMAN	SAMD9	physical	26496610
CTF8_HUMAN	CHTF8	physical	26496610
APC1_HUMAN	ANAPC1	physical	26496610
GKAP1_HUMAN	GKAP1	physical	26496610
APC16_HUMAN	ANAPC16	physical	26496610
CDC26_HUMAN	CDC26	physical	26496610
MEF2C_MOUSE	Mef2c	physical	25789873

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.