dbPTM

MARK1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MARK1_HUMAN
UniProt AC	Q9P0L2
Protein Name	Serine/threonine-protein kinase MARK1
Gene Name	MARK1 {ECO:0000312\|HGNC:HGNC:6896}
Organism	Homo sapiens (Human).
Sequence Length	795
Subcellular Localization	Cell membrane Peripheral membrane protein . Cytoplasm, cytoskeleton. Cytoplasm . Cell projection, dendrite . Appears to localize to an intracellular network..
Protein Description	Serine/threonine-protein kinase. [PubMed: 23666762 Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU]
Protein Sequence	MSARTPLPTVNERDTENHTSVDGYTEPHIQPTKSSSRQNIPRCRNSITSATDEQPHIGNYRLQKTIGKGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIVHRDLKAENLLLDGDMNIKIADFGFSNEFTVGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYTEPDPDFNDTKRIDIMVTMGFARDEINDALINQKYDEVMATYILLGRKPPEFEGGESLSSGNLCQRSRPSSDLNNSTLQSPAHLKVQRSISANQKQRRFSDHAGPSIPPAVSYTKRPQANSVESEQKEEWDKDVARKLGSTTVGSKSEMTASPLVGPERKKSSTIPSNNVYSGGSMARRNTYVCERTTDRYVALQNGKDSSLTEMSVSSISSAGSSVASAVPSARPRHQKSMSTSGHPIKVTLPTIKDGSEAYRPGTTQRVPAASPSAHSISTATPDRTRFPRGSSSRSTFHGEQLRERRSVAYNGPPASPSHETGAFAHARRGTSTGIISKITSKFVRRDPSEGEASGRTDTSRSTSGEPKERDKEEGKDSKPRSLRFTWSMKTTSSMDPNDMMREIRKVLDANNCDYEQKERFLLFCVHGDARQDSLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASKIANELKL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Phosphorylation	---MSARTPLPTVNE ---CCCCCCCCCCCC	28.97	17192257
15	Phosphorylation	PTVNERDTENHTSVD CCCCCCCCCCCCCCC	44.93	20873877
19	Phosphorylation	ERDTENHTSVDGYTE CCCCCCCCCCCCCCC	41.86	20873877
20	Phosphorylation	RDTENHTSVDGYTEP CCCCCCCCCCCCCCC	15.62	30576142
24	Phosphorylation	NHTSVDGYTEPHIQP CCCCCCCCCCCCCCC	11.94	29978859
25	Phosphorylation	HTSVDGYTEPHIQPT CCCCCCCCCCCCCCC	48.87	29978859
34	Phosphorylation	PHIQPTKSSSRQNIP CCCCCCCCCCCCCCC	35.70	30576142
36	Phosphorylation	IQPTKSSSRQNIPRC CCCCCCCCCCCCCCH	45.00	-
46	Phosphorylation	NIPRCRNSITSATDE CCCCHHHCCCCCCCC	14.11	21082442
48	Phosphorylation	PRCRNSITSATDEQP CCHHHCCCCCCCCCC	16.48	20873877
49	Phosphorylation	RCRNSITSATDEQPH CHHHCCCCCCCCCCC	27.74	29978859
51	Phosphorylation	RNSITSATDEQPHIG HHCCCCCCCCCCCCC	39.58	29978859
65	Phosphorylation	GNYRLQKTIGKGNFA CCEEECEEECCCCHH	23.23	28152594
68	Ubiquitination	RLQKTIGKGNFAKVK EECEEECCCCHHHHH	46.99	-
89	Ubiquitination	TGREVAVKIIDKTQL CCCHHHEEECCCCCC	24.98	-
93	Ubiquitination	VAVKIIDKTQLNPTS HHEEECCCCCCCHHH	27.97	-
94	Phosphorylation	AVKIIDKTQLNPTSL HEEECCCCCCCHHHH	34.24	20068231
99	Phosphorylation	DKTQLNPTSLQKLFR CCCCCCHHHHHHHHH	40.78	-
100	Phosphorylation	KTQLNPTSLQKLFRE CCCCCHHHHHHHHHH	30.02	-
131	Phosphorylation	EVIETEKTLYLVMEY EEECCCCEEEEEEHH	17.88	25921289
140	Phosphorylation	YLVMEYASGGEVFDY EEEEHHHCCHHHHHH	46.56	25921289
184	Ubiquitination	YIVHRDLKAENLLLD CCCCCCCCHHHEEEC	58.92	21906983
204	Phosphorylation	KIADFGFSNEFTVGN EECCCCCCCCEECCC	35.51	30206219
208	Phosphorylation	FGFSNEFTVGNKLDT CCCCCCEECCCCHHH	22.71	17573348
215	Phosphorylation	TVGNKLDTFCGSPPY ECCCCHHHCCCCCCC	31.12	14976552
219	Phosphorylation	KLDTFCGSPPYAAPE CHHHCCCCCCCCCHH	24.71	19664994
222	Phosphorylation	TFCGSPPYAAPELFQ HCCCCCCCCCHHHHC	20.84	23898821
231	Ubiquitination	APELFQGKKYDGPEV CHHHHCCCCCCCCCC	37.06	-
279	Phosphorylation	GKYRIPFYMSTDCEN CCCCCCEEECCCHHH	5.70	29496907
281	Phosphorylation	YRIPFYMSTDCENLL CCCCEEECCCHHHHH	15.09	21082442
282	Phosphorylation	RIPFYMSTDCENLLK CCCEEECCCHHHHHH	28.30	21082442
301	Phosphorylation	LNPIKRGSLEQIMKD HCCCCCCCHHHHHHH	32.53	25332170
307	Ubiquitination	GSLEQIMKDRWMNVG CCHHHHHHHCCCCCC	45.85	-
333	Phosphorylation	PDPDFNDTKRIDIMV CCCCCCCCCCEEEEE	24.27	19369195
380	Phosphorylation	PEFEGGESLSSGNLC CCCCCCCCCCCCCCC	36.50	27251275
382	Phosphorylation	FEGGESLSSGNLCQR CCCCCCCCCCCCCCC	45.54	20873877
383	Phosphorylation	EGGESLSSGNLCQRS CCCCCCCCCCCCCCC	36.67	27251275
390	Phosphorylation	SGNLCQRSRPSSDLN CCCCCCCCCCCCCCC	23.75	23403867
393	Phosphorylation	LCQRSRPSSDLNNST CCCCCCCCCCCCCCC	35.06	23898821
394	Phosphorylation	CQRSRPSSDLNNSTL CCCCCCCCCCCCCCC	49.12	21082442
399	Phosphorylation	PSSDLNNSTLQSPAH CCCCCCCCCCCCHHH	29.44	25159151
400	Phosphorylation	SSDLNNSTLQSPAHL CCCCCCCCCCCHHHH	30.82	23403867
403	Phosphorylation	LNNSTLQSPAHLKVQ CCCCCCCCHHHHHHH	27.45	25159151
412	Phosphorylation	AHLKVQRSISANQKQ HHHHHHHHCCCHHHC	12.10	29514088
414	Phosphorylation	LKVQRSISANQKQRR HHHHHHCCCHHHCCC	23.55	29900121
423	Phosphorylation	NQKQRRFSDHAGPSI HHHCCCCCCCCCCCC	27.03	25159151
429	Phosphorylation	FSDHAGPSIPPAVSY CCCCCCCCCCCCCCC	46.97	27732954
444	Phosphorylation	TKRPQANSVESEQKE CCCCCCCCCCHHHHH	30.22	25159151
463	Phosphorylation	DVARKLGSTTVGSKS HHHHHHCCCCCCCCC	31.20	28857561
464	Phosphorylation	VARKLGSTTVGSKSE HHHHHCCCCCCCCCC	24.57	28857561
465	Phosphorylation	ARKLGSTTVGSKSEM HHHHCCCCCCCCCCC	25.27	28857561
468	Phosphorylation	LGSTTVGSKSEMTAS HCCCCCCCCCCCCCC	28.77	25159151
470	Phosphorylation	STTVGSKSEMTASPL CCCCCCCCCCCCCCC	34.01	28857561
473	Phosphorylation	VGSKSEMTASPLVGP CCCCCCCCCCCCCCC	21.67	28857561
475	Phosphorylation	SKSEMTASPLVGPER CCCCCCCCCCCCCCC	15.03	25159151
485	Phosphorylation	VGPERKKSSTIPSNN CCCCCCCCCCCCCCC	35.43	21945579
486	Phosphorylation	GPERKKSSTIPSNNV CCCCCCCCCCCCCCC	38.96	21945579
487	Phosphorylation	PERKKSSTIPSNNVY CCCCCCCCCCCCCCC	43.95	21945579
490	Phosphorylation	KKSSTIPSNNVYSGG CCCCCCCCCCCCCCC	36.87	21945579
494	Phosphorylation	TIPSNNVYSGGSMAR CCCCCCCCCCCCCHH	12.06	21945579
495	Phosphorylation	IPSNNVYSGGSMARR CCCCCCCCCCCCHHC	32.18	21945579
498	Phosphorylation	NNVYSGGSMARRNTY CCCCCCCCCHHCCEE	16.88	21945579
504	Phosphorylation	GSMARRNTYVCERTT CCCHHCCEEEEECCC	18.60	28857561
505	Phosphorylation	SMARRNTYVCERTTD CCHHCCEEEEECCCC	13.47	23312004
514	Phosphorylation	CERTTDRYVALQNGK EECCCCCEEEEECCC	7.90	27642862
514 (in isoform 2)	Phosphorylation	-	7.90	25159151
523	Phosphorylation	ALQNGKDSSLTEMSV EEECCCCCCCCEEEH	30.63	22496350
524	Phosphorylation	LQNGKDSSLTEMSVS EECCCCCCCCEEEHH	49.82	27732954
526	Phosphorylation	NGKDSSLTEMSVSSI CCCCCCCCEEEHHHH	31.43	27732954
529	Phosphorylation	DSSLTEMSVSSISSA CCCCCEEEHHHHHHC	16.77	27732954
531	Phosphorylation	SLTEMSVSSISSAGS CCCEEEHHHHHHCCH	18.04	27732954
532	Phosphorylation	LTEMSVSSISSAGSS CCEEEHHHHHHCCHH	25.08	27732954
534	Phosphorylation	EMSVSSISSAGSSVA EEEHHHHHHCCHHHH	18.98	27732954
535	Phosphorylation	MSVSSISSAGSSVAS EEHHHHHHCCHHHHH	34.67	27732954
538	Phosphorylation	SSISSAGSSVASAVP HHHHHCCHHHHHHCC	22.74	29449344
554	Phosphorylation	ARPRHQKSMSTSGHP CCCCCCCCCCCCCCC	16.05	24719451
556	Phosphorylation	PRHQKSMSTSGHPIK CCCCCCCCCCCCCCE	26.89	28857561
557	Phosphorylation	RHQKSMSTSGHPIKV CCCCCCCCCCCCCEE	30.04	27732954
558	Phosphorylation	HQKSMSTSGHPIKVT CCCCCCCCCCCCEEE	28.00	27732954
573	Phosphorylation	LPTIKDGSEAYRPGT ECCCCCCCCCCCCCC	29.23	24719451
580	Phosphorylation	SEAYRPGTTQRVPAA CCCCCCCCCCCCCCC	23.57	-
581	Phosphorylation	EAYRPGTTQRVPAAS CCCCCCCCCCCCCCC	21.94	-
588	Phosphorylation	TQRVPAASPSAHSIS CCCCCCCCCCCCCCC	22.84	26055452
590	Phosphorylation	RVPAASPSAHSISTA CCCCCCCCCCCCCCC	35.80	21712546
593	Phosphorylation	AASPSAHSISTATPD CCCCCCCCCCCCCCC	20.25	23403867
595	Phosphorylation	SPSAHSISTATPDRT CCCCCCCCCCCCCCC	18.27	23403867
596	Phosphorylation	PSAHSISTATPDRTR CCCCCCCCCCCCCCC	32.64	21712546
598	Phosphorylation	AHSISTATPDRTRFP CCCCCCCCCCCCCCC	26.30	23403867
602	Phosphorylation	STATPDRTRFPRGSS CCCCCCCCCCCCCCC	44.00	29449344
612	Phosphorylation	PRGSSSRSTFHGEQL CCCCCCCCCCCHHHH	37.06	19276368
613	Phosphorylation	RGSSSRSTFHGEQLR CCCCCCCCCCHHHHH	20.67	21712546
624	Phosphorylation	EQLRERRSVAYNGPP HHHHHHHCHHHCCCC	19.81	30177828
627	Phosphorylation	RERRSVAYNGPPASP HHHHCHHHCCCCCCC	20.63	23186163
627 (in isoform 3)	Phosphorylation	-	20.63	25159151
633	Phosphorylation	AYNGPPASPSHETGA HHCCCCCCCCHHCCC	32.48	25159151
635	Phosphorylation	NGPPASPSHETGAFA CCCCCCCCHHCCCCC	31.31	29978859
638	Phosphorylation	PASPSHETGAFAHAR CCCCCHHCCCCCHHH	28.52	28857561
646	Methylation	GAFAHARRGTSTGII CCCCHHHCCCCCCHH	54.23	82797037
648	Phosphorylation	FAHARRGTSTGIISK CCHHHCCCCCCHHHH	22.70	25159151
649	Phosphorylation	AHARRGTSTGIISKI CHHHCCCCCCHHHHH	27.71	25159151
650	Phosphorylation	HARRGTSTGIISKIT HHHCCCCCCHHHHHH	32.03	22496350
654	Phosphorylation	GTSTGIISKITSKFV CCCCCHHHHHHHHHC	18.98	23312004
666	Phosphorylation	KFVRRDPSEGEASGR HHCCCCCCCCCCCCC	63.62	-
679	Phosphorylation	GRTDTSRSTSGEPKE CCCCCCCCCCCCCCC	27.05	-
681	Phosphorylation	TDTSRSTSGEPKERD CCCCCCCCCCCCCCC	42.26	-
699	Phosphorylation	GKDSKPRSLRFTWSM CCCCCCCEEEEEEEC	32.21	20860994
703	Phosphorylation	KPRSLRFTWSMKTTS CCCEEEEEEECEECC	15.36	29759185
705	Phosphorylation	RSLRFTWSMKTTSSM CEEEEEEECEECCCC	13.82	-
708	Phosphorylation	RFTWSMKTTSSMDPN EEEEECEECCCCCHH	24.22	21130716
709	Phosphorylation	FTWSMKTTSSMDPND EEEECEECCCCCHHH	17.18	21130716
710	Phosphorylation	TWSMKTTSSMDPNDM EEECEECCCCCHHHH	28.97	21130716
711	Phosphorylation	WSMKTTSSMDPNDMM EECEECCCCCHHHHH	25.72	21130716
723	Ubiquitination	DMMREIRKVLDANNC HHHHHHHHHHHHCCC	53.46	-
746 (in isoform 3)	Ubiquitination	-	24.57	21890473
766	Phosphorylation	VCKLPRLSLNGVRFK HHCCCCEEECCEEEE	22.52	27067055
768 (in isoform 2)	Ubiquitination	-	43.46	21890473
776	Phosphorylation	GVRFKRISGTSIAFK CEEEEECCCCHHHHH	39.80	29514088
778	Phosphorylation	RFKRISGTSIAFKNI EEEECCCCHHHHHHH	15.35	29514088
779	Phosphorylation	FKRISGTSIAFKNIA EEECCCCHHHHHHHH	18.55	29514088
783	Ubiquitination	SGTSIAFKNIASKIA CCCHHHHHHHHHHHH	38.12	-
783 (in isoform 1)	Ubiquitination	-	38.12	21890473
787	Phosphorylation	IAFKNIASKIANELK HHHHHHHHHHHHHHC	22.31	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
215	T	Phosphorylation	Kinase	STK11	Q15831	PhosphoELM
215	T	Phosphorylation	Kinase	TAOK1	Q7L7X3	Uniprot
219	S	Phosphorylation	Kinase	GSK3B	P49841	GPS
613	T	Phosphorylation	Kinase	PRKCZ	Q05513	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
215	T	Phosphorylation	14976552
Phosphorylated at Thr-215 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.
215	T	Phosphorylation	14976552
Phosphorylation at Thr-215 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules.
219	S	Phosphorylation	17573348
Phosphorylation at Ser-219 by GSK3-beta (GSK3B) inhibits the kinase activity.
613	T	Phosphorylation	17573348
Phosphorylation at Thr-613 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MARK1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MTAP2_RAT	Map2	physical	8631898
MAP4_MOUSE	Map4	physical	8631898
TAU_HUMAN	MAPT	physical	8631898
TAU_HUMAN	MAPT	physical	10090741
TAU_HUMAN	MAPT	physical	23666762
C102B_HUMAN	CCDC102B	physical	24722188

Drug and Disease Associations

Kegg Disease
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MARK1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-463; SER-468; SER-470;SER-475; SER-588 AND SER-633, AND MASS SPECTROMETRY.	19369195 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-588 AND THR-596, ANDMASS SPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-5; SER-46; THR-215;SER-219; SER-403; SER-423; SER-475; SER-588 AND SER-633, AND MASSSPECTROMETRY.	18691976 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-588 AND THR-598, ANDMASS SPECTROMETRY.	18220336 [Abstract]
"Proteomics analysis of protein kinases by target class-selectiveprefractionation and tandem mass spectrometry."; Wissing J., Jaensch L., Nimtz M., Dieterich G., Hornberger R.,Keri G., Wehland J., Daub H.; Mol. Cell. Proteomics 6:537-547(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-649, AND MASSSPECTROMETRY.	17192257 [Abstract]
"LKB1 is a master kinase that activates 13 kinases of the AMPKsubfamily, including MARK/PAR-1."; Lizcano J.M., Goeransson O., Toth R., Deak M., Morrice N.A.,Boudeau J., Hawley S.A., Udd L., Maekelae T.P., Hardie D.G.,Alessi D.R.; EMBO J. 23:833-843(2004). Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME REGULATION,PHOSPHORYLATION AT THR-215, AND MUTAGENESIS OF THR-215.	14976552 [Abstract]

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