dbPTM

SRF_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SRF_HUMAN
UniProt AC	P11831
Protein Name	Serum response factor
Gene Name	SRF
Organism	Homo sapiens (Human).
Sequence Length	508
Subcellular Localization	Nucleus .
Protein Description	SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation..
Protein Sequence	MLPTQAGAAAALGRGSALGGSLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGPGRLEREAAAAAATTPAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSEMEIGMVVGGPEASAAATGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATGFEETDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPSTSTTMQVSSGPSFPITNYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPGGAVAQQVPVQAIQVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPTSGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAVIGQQAGSSSNLTELQVVNLDTAHSTKSE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Phosphorylation	----MLPTQAGAAAA ----CCCCHHHHHHH	27.39	23532336
14	Methylation	GAAAALGRGSALGGS HHHHHHCCCCCCCCC	36.42	115384247
16	Phosphorylation	AAALGRGSALGGSLN HHHHCCCCCCCCCCC	20.96	23186163
21	Phosphorylation	RGSALGGSLNRTPTG CCCCCCCCCCCCCCC	22.22	23186163
24	Methylation	ALGGSLNRTPTGRPG CCCCCCCCCCCCCCC	47.58	115384255
25	Phosphorylation	LGGSLNRTPTGRPGG CCCCCCCCCCCCCCC	24.91	23186163
27	Phosphorylation	GSLNRTPTGRPGGGG CCCCCCCCCCCCCCC	45.25	23186163
36	O-linked_Glycosylation	RPGGGGGTRGANGGR CCCCCCCCCCCCCCC	29.24	23301498
37	Methylation	PGGGGGTRGANGGRV CCCCCCCCCCCCCCC	45.56	115917745
66	Phosphorylation	EAAAAAATTPAPTAG HHHHHHCCCCCCCCC	28.69	20873877
67	Phosphorylation	AAAAAATTPAPTAGA HHHHHCCCCCCCCCC	16.84	20873877
71	Phosphorylation	AATTPAPTAGALYSG HCCCCCCCCCCEECC	39.57	28450419
76	Phosphorylation	APTAGALYSGSEGDS CCCCCCEECCCCCCC	15.51	22115753
77	Phosphorylation	PTAGALYSGSEGDSE CCCCCEECCCCCCCC	36.37	19778940
79	Phosphorylation	AGALYSGSEGDSESG CCCEECCCCCCCCCC	32.25	19778940
83	Phosphorylation	YSGSEGDSESGEEEE ECCCCCCCCCCCHHH	44.51	19778940
85	Phosphorylation	GSEGDSESGEEEELG CCCCCCCCCCHHHHH	56.29	10318869
101	Phosphorylation	ERRGLKRSLSEMEIG HHHCHHHHHHHCEEE	34.36	25159151
103	Phosphorylation	RGLKRSLSEMEIGMV HCHHHHHHHCEEEEE	35.82	25159151
117	Phosphorylation	VVGGPEASAAATGGY EECCHHHHHHHHCCC	19.44	30108239
121	Phosphorylation	PEASAAATGGYGPVS HHHHHHHHCCCCCCC	26.81	28464451
124	Phosphorylation	SAAATGGYGPVSGAV HHHHHCCCCCCCCCC	21.43	26074081
128	Phosphorylation	TGGYGPVSGAVSGAK HCCCCCCCCCCCCCC	24.94	26074081
132	Phosphorylation	GPVSGAVSGAKPGKK CCCCCCCCCCCCCCC	32.28	26074081
147	Sumoylation	TRGRVKIKMEFIDNK CCCCEEEEEEECCHH	27.02	-
147	Sumoylation	TRGRVKIKMEFIDNK CCCCEEEEEEECCHH	27.02	-
159	Phosphorylation	DNKLRRYTTFSKRKT CHHHHHHCCCCCCCC	21.39	12809504
160	Phosphorylation	NKLRRYTTFSKRKTG HHHHHHCCCCCCCCC	19.58	10753652
162	Phosphorylation	LRRYTTFSKRKTGIM HHHHCCCCCCCCCCC	29.47	22817900
173	Phosphorylation	TGIMKKAYELSTLTG CCCCHHHHHHHHCCC	26.70	24043423
176	Phosphorylation	MKKAYELSTLTGTQV CHHHHHHHHCCCCEE	14.94	26552605
177	Phosphorylation	KKAYELSTLTGTQVL HHHHHHHHCCCCEEE	40.75	26552605
179	Phosphorylation	AYELSTLTGTQVLLL HHHHHHCCCCEEEEE	37.97	24043423
181	Phosphorylation	ELSTLTGTQVLLLVA HHHHCCCCEEEEEEE	15.40	24043423
189	Phosphorylation	QVLLLVASETGHVYT EEEEEEEECCCCEEE	28.36	24043423
191	Phosphorylation	LLLVASETGHVYTFA EEEEEECCCCEEEEE	30.05	24043423
195	Phosphorylation	ASETGHVYTFATRKL EECCCCEEEEECCCC	7.17	24043423
196	Phosphorylation	SETGHVYTFATRKLQ ECCCCEEEEECCCCC	13.00	24043423
199	Phosphorylation	GHVYTFATRKLQPMI CCEEEEECCCCCCCE	24.93	26552605
210	Phosphorylation	QPMITSETGKALIQT CCCEECCHHHHHHHH	43.69	26074081
217	Phosphorylation	TGKALIQTCLNSPDS HHHHHHHHHHCCCCC	16.39	23927012
221	Phosphorylation	LIQTCLNSPDSPPRS HHHHHHCCCCCCCCC	19.69	29255136
224	Phosphorylation	TCLNSPDSPPRSDPT HHHCCCCCCCCCCCC	39.94	19664994
228	Phosphorylation	SPDSPPRSDPTTDQR CCCCCCCCCCCCCCC	54.43	22167270
231	Phosphorylation	SPPRSDPTTDQRMSA CCCCCCCCCCCCHHC	48.25	22167270
232	Phosphorylation	PPRSDPTTDQRMSAT CCCCCCCCCCCHHCC	35.21	22167270
237	Phosphorylation	PTTDQRMSATGFEET CCCCCCHHCCCCEEC	25.52	26074081
239	Phosphorylation	TDQRMSATGFEETDL CCCCHHCCCCEECCE	34.79	26074081
251	Phosphorylation	TDLTYQVSESDSSGE CCEEEEEECCCCCCC	18.83	22817900
253	Phosphorylation	LTYQVSESDSSGETK EEEEEECCCCCCCCC	34.97	22817900
277	O-linked_Glycosylation	TNLPGTTSTIQTAPS ECCCCCCCEEEECCC	24.29	1512232
277	O-linked_Glycosylation	TNLPGTTSTIQTAPS ECCCCCCCEEEECCC	24.29	23301498
288	O-linked_Glycosylation	TAPSTSTTMQVSSGP ECCCCCCEEEEECCC	12.84	23301498
307	O-linked_Glycosylation	TNYLAPVSASVSPSA CCCEEEEEECCCHHH	17.48	23301498
307	Phosphorylation	TNYLAPVSASVSPSA CCCEEEEEECCCHHH	17.48	26074081
309	Phosphorylation	YLAPVSASVSPSAVS CEEEEEECCCHHHHH	18.67	26074081
309	O-linked_Glycosylation	YLAPVSASVSPSAVS CEEEEEECCCHHHHH	18.67	23301498
311	O-linked_Glycosylation	APVSASVSPSAVSSA EEEEECCCHHHHHCC	15.61	23301498
311	Phosphorylation	APVSASVSPSAVSSA EEEEECCCHHHHHCC	15.61	26074081
313	O-linked_Glycosylation	VSASVSPSAVSSANG EEECCCHHHHHCCCC	33.52	23301498
313	Phosphorylation	VSASVSPSAVSSANG EEECCCHHHHHCCCC	33.52	26074081
316	O-linked_Glycosylation	SVSPSAVSSANGTVL CCCHHHHHCCCCCEE	24.37	23301498
316	O-linked_Glycosylation	SVSPSAVSSANGTVL CCCHHHHHCCCCCEE	24.37	1512232
316	Phosphorylation	SVSPSAVSSANGTVL CCCHHHHHCCCCCEE	24.37	26074081
317	Phosphorylation	VSPSAVSSANGTVLK CCHHHHHCCCCCEEE	21.10	26074081
370	Phosphorylation	HQAPQQASPSRDSST ECCCCCCCCCCCCCC	21.04	26657352
372	Phosphorylation	APQQASPSRDSSTDL CCCCCCCCCCCCCCC	46.34	27080861
383	O-linked_Glycosylation	STDLTQTSSSGTVTL CCCCEECCCCCEEEE	16.82	23301498
384	O-linked_Glycosylation	TDLTQTSSSGTVTLP CCCEECCCCCEEEEE	35.85	-
385	O-linked_Glycosylation	DLTQTSSSGTVTLPA CCEECCCCCEEEEEE	37.95	-
401	O-linked_Glycosylation	IMTSSVPTTVGGHMM EEECCCCCEECCEEC	31.77	23301498
435	Phosphorylation	LTVLNAFSQAPSTMQ EEHHHHHHCCCCEEE	24.03	22817900
446	Phosphorylation	STMQVSHSQVQEPGG CEEEECHHHCCCCCC	25.25	1512232
460	O-linked_Glycosylation	GVPQVFLTASSGTVQ CCCEEEEEECCCEEE	17.02	23301498
462	O-linked_Glycosylation	PQVFLTASSGTVQIP CEEEEEECCCEEEEE	24.86	23301498
463	O-linked_Glycosylation	QVFLTASSGTVQIPV EEEEEECCCEEEEEC	35.70	23301498
465	O-linked_Glycosylation	FLTASSGTVQIPVSA EEEECCCEEEEECCE	15.93	23301498
471	O-linked_Glycosylation	GTVQIPVSAVQLHQM CEEEEECCEEEEEEE	19.85	23301498

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
77	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
79	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
83	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
85	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
85	S	Phosphorylation	Kinase	MAPKAPK2	P49137	GPS
103	S	Phosphorylation	Kinase	RSK_GROUP	-	PhosphoELM
103	S	Phosphorylation	Kinase	RSK-SUBFAMILY	-	GPS
103	S	Phosphorylation	Kinase	MAPKAPK2	P49137	PSP
103	S	Phosphorylation	Kinase	RPS6KA5	O75582	GPS
103	S	Phosphorylation	Kinase	RPS6KA1	P18653	GPS
103	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
159	T	Phosphorylation	Kinase	PRKCA	P17252	GPS
159	T	Phosphorylation	Kinase	PRKG1	Q13976	GPS
159	T	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
159	T	Phosphorylation	Kinase	PRKACA	P17612	GPS
159	T	Phosphorylation	Kinase	DMPK_GROUP	-	PhosphoELM
159	T	Phosphorylation	Kinase	DMPK	Q09013	GPS
160	T	Phosphorylation	Kinase	CAMK2A	Q9UQM7	GPS
162	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
435	S	Phosphorylation	Kinase	PRKDC	P78527	PhosphoELM
435	S	Phosphorylation	Kinase	DSDNA KINASE	-	Uniprot
446	S	Phosphorylation	Kinase	PRKDC	P78527	PhosphoELM
446	S	Phosphorylation	Kinase	DSDNA KINASE	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of SRF_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SRF_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CBP_HUMAN	CREBBP	physical	12622724
PML_HUMAN	PML	physical	12622724
SSRP1_RAT	Ssrp1	physical	10336466
SSRP1_HUMAN	SSRP1	physical	10336466
GATA4_HUMAN	GATA4	physical	11003651
REL_HUMAN	REL	physical	8670842
TF65_HUMAN	RELA	physical	8670842
NFYA_HUMAN	NFYA	physical	10571058
T2FA_HUMAN	GTF2F1	physical	7854423
GATA4_HUMAN	GATA4	physical	11158291
NKX31_HUMAN	NKX3-1	physical	10993896
MYOG_HUMAN	MYOG	physical	8617811
MYOG_HUMAN	MYOG	physical	10082523
NCOR2_HUMAN	NCOR2	physical	10777532
GTF2I_HUMAN	GTF2I	physical	9334314
NKX25_MOUSE	Nkx2-5	physical	8887666
HMGA1_MOUSE	Hmga1	genetic	9545312
HMGA2_MOUSE	Hmga2	genetic	9545312
HMGA1_MOUSE	Hmga1	physical	9545312
CD63_HUMAN	CD63	physical	9271374
ATF6A_HUMAN	ATF6	physical	9271374
ELK4_HUMAN	ELK4	physical	9271374
MKL1_MOUSE	Mkl1	physical	12397177
MKL2_MOUSE	Mkl2	physical	12397177
MYCD_MOUSE	Myocd	physical	12397177
GATA2_RAT	Gata2	genetic	12530967
GATA4_RAT	Gata4	genetic	12530967
GATA6_RAT	Gata6	genetic	12530967
GATA6_RAT	Gata6	physical	12530967
GATA4_RAT	Gata4	physical	12530967
CEBPB_HUMAN	CEBPB	physical	10318842
CEBPB_HUMAN	CEBPB	physical	9032301
SMCA4_HUMAN	SMARCA4	physical	19342595
MYCD_HUMAN	MYOCD	physical	19342595
HDAC3_HUMAN	HDAC3	physical	17158926
SMAD7_HUMAN	SMAD7	physical	16690609
EPC1_HUMAN	EPC1	physical	19359245
HDAC4_HUMAN	HDAC4	physical	12663674
PELP1_HUMAN	PELP1	physical	15456770
SMCA4_HUMAN	SMARCA4	physical	17599918
MKL1_HUMAN	MKL1	physical	17599918
SRF_HUMAN	SRF	physical	2046671
KDM6A_HUMAN	KDM6A	physical	22192413
NFKB1_HUMAN	NFKB1	physical	2108863
SRF_HUMAN	SRF	physical	2108863
SRF_HUMAN	SRF	physical	8083237
ELK1_HUMAN	ELK1	physical	8083237
SRFB1_HUMAN	SRFBP1	physical	8083237
FHL2_HUMAN	FHL2	physical	15610731
MKL1_HUMAN	MKL1	physical	15610731
MYCD_HUMAN	MYOCD	physical	17360478
TRIP4_HUMAN	TRIP4	physical	12077347
NCOA1_HUMAN	NCOA1	physical	9786846

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SRF_HUMAN

Related Literatures of Post-Translational Modification

O-linked Glycosylation
Reference	PubMed
"Localization of O-GlcNAc modification on the serum responsetranscription factor."; Reason A.J., Morris H.R., Panico M., Marais R., Treisman R.H.,Haltiwanger R.S., Hart G.W., Kelly W.G., Dell A.; J. Biol. Chem. 267:16911-16921(1992). Cited for: GLYCOSYLATION AT SER-277; SER-307; SER-309; SER-316 AND SER-383.	1512232 [Abstract]
Phosphorylation
Reference	PubMed
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; SER-79; SER-83;SER-85 AND SER-224, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-76; SER-77; SER-79;SER-83 AND SER-224, AND MASS SPECTROMETRY.	17081983 [Abstract]
"The carboxyl-terminal transactivation domain of human serum responsefactor contains DNA-activated protein kinase phosphorylation sites."; Liu S.-H., Ma J.-T., Yueh A.Y., Lees-Miller S.P., Anderson C.W.,Ng S.-Y.; J. Biol. Chem. 268:21147-21154(1993). Cited for: PHOSPHORYLATION AT SER-435 AND SER-446.	8407951 [Abstract]
"Identification of multiple SRF N-terminal phosphorylation sitesaffecting DNA binding properties."; Janknecht R., Hipskind R.A., Houthaeve T., Nordheim A.,Stunnenberg H.G.; EMBO J. 11:1045-1054(1992). Cited for: PHOSPHORYLATION AT SER-77; SER-79; SER-83; SER-85 AND SER-103.	1547771 [Abstract]
"C-terminal phosphorylation of the serum-response factor."; Janknecht R., Ernst W.H., Houthaeve T., Nordheim A.; Eur. J. Biochem. 216:469-475(1993). Cited for: PROTEIN SEQUENCE OF 210-221 AND 253-264, AND PHOSPHORYLATION ATSER-253.	8375385 [Abstract]