SSRP1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SSRP1_RAT
• UniProt AC: Q04931
• Protein Name: FACT complex subunit SSRP1
• Gene Name: Ssrp1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 709
• Subcellular Localization: Nucleus . Chromosome . Nucleus, nucleolus . Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
• Protein Description: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63..
• Protein Sequence: MAETLEFNDIFQEVKGSMNDGRLRLSRQGIIFKNSKTGKVDNIQAGELTEGIWRRVALGHGLKLLTKNGHVYKYDGFRESEFEKLSDFFKTHYRLELMEKDLCVKGWNWGTVKFGGQLLSFDIGDQPVFEIPLSNVSQCTTGKNEVTLEFHQNDDAEVSLMEVRFYVPPTQEDGVDPVEAFAQNVLSKADVIQATGDAICIFRELQCLTPRGRYDIRIYPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKDEDISLTLNMNEEEVEKRFEGRLTKNMSGSLYEMVSRVMKALVNRKITVPGNFQGHSGAQCITCSYKASSGLLYPLERGFIYVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEGINPGYDDYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEGDSDREEKKRKQLKRAKMAKDRKSRKKSSEGKKGKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARREYEKAMKEYEGGRGDSSKRDKSKKKKKVKAKLEKKSTPSRGSSSKSSSRQLSDSFKSKEFVSSDESSSGENKSKKKRRRSEDSDEEELASTPPSSEDSASGSDE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAETLEFND ------CCCEECHHH	20.62	-
84	Ubiquitination	FRESEFEKLSDFFKT CCHHHHHHHHHHHHH	59.62	-
100	Ubiquitination	YRLELMEKDLCVKGW HHHHHHHHCEEECCC	41.87	-
105	Ubiquitination	MEKDLCVKGWNWGTV HHHCEEECCCCCEEE	58.15	-
111	Phosphorylation	VKGWNWGTVKFGGQL ECCCCCEEEEECCEE	16.49	23984901
170	Phosphorylation	VRFYVPPTQEDGVDP EEEECCCCCCCCCCH	38.22	-
233	Acetylation	HGKTFDYKIPYTTVL CCCCCCCCCCCHHHE	38.76	22902405
413	Acetylation	IEREEYGKLFDFVNA EEHHHHHHHHHHHCH	45.11	-
438	Phosphorylation	KEGINPGYDDYADSD CCCCCCCCCCCCCCC	13.60	25403869
441	Phosphorylation	INPGYDDYADSDEDQ CCCCCCCCCCCCHHH	14.92	27097102
444	Phosphorylation	GYDDYADSDEDQHDA CCCCCCCCCHHHHHH	34.43	23712012
452	Phosphorylation	DEDQHDAYLERMKEE CHHHHHHHHHHHHHH	18.36	27097102
471	Phosphorylation	EENANDSSDDSGEET CCCCCCCCCCCCCCC	48.58	-
510	Phosphorylation	SSSNEGDSDREEKKR CCCCCCCCHHHHHHH	49.80	-
536	Phosphorylation	RKSRKKSSEGKKGKD HHHHHHCCCCCCCCC	60.28	25532521
542	Acetylation	SSEGKKGKDPNAPKR CCCCCCCCCCCCCCC	78.04	-
657	Phosphorylation	KSSSRQLSDSFKSKE CHHHCHHCHHHHCHH	23.91	22108457
659	Phosphorylation	SSRQLSDSFKSKEFV HHCHHCHHHHCHHHH	31.06	22108457
661	Acetylation	RQLSDSFKSKEFVSS CHHCHHHHCHHHHCC	65.65	22902405
662	Phosphorylation	QLSDSFKSKEFVSSD HHCHHHHCHHHHCCC	35.09	28432305
667	Phosphorylation	FKSKEFVSSDESSSG HHCHHHHCCCCCCCC	37.08	28432305
668	Phosphorylation	KSKEFVSSDESSSGE HCHHHHCCCCCCCCC	39.84	28432305
671	Phosphorylation	EFVSSDESSSGENKS HHHCCCCCCCCCCHH	34.49	28432305
672	Phosphorylation	FVSSDESSSGENKSK HHCCCCCCCCCCHHH	40.07	21630457
673	Phosphorylation	VSSDESSSGENKSKK HCCCCCCCCCCHHHH	60.45	28432305
688	Phosphorylation	KRRRSEDSDEEELAS CCCCCCCCCHHHHHC	42.76	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
510	S	Phosphorylation	Kinase	CK2	-	Uniprot
657	S	Phosphorylation	Kinase	CK2	-	Uniprot
688	S	Phosphorylation	Kinase	CK2	-	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SSRP1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SSRP1_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of SSRP1_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.