dbPTM

GATA4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	GATA4_HUMAN
UniProt AC	P43694
Protein Name	Transcription factor GATA-4
Gene Name	GATA4
Organism	Homo sapiens (Human).
Sequence Length	442
Subcellular Localization	Nucleus .
Protein Description	Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function. [PubMed: 24000169]
Protein Sequence	MYQSLAMAANHGPPPGAYEAGGPGAFMHGAGAASSPVYVPTPRVPSSVLGLSYLQGGGAGSASGGASGGSSGGAASGAGPGTQQGSPGWSQAGADGAAYTPPPVSPRFSFPGTTGSLAAAAAAAAAREAAAYSSGGGAAGAGLAGREQYGRAGFAGSYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRANPAARHPNLDMFDDFSEGRECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPASGASSNSSNATTSSSEEMRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
34	Phosphorylation	MHGAGAASSPVYVPT CCCCCCCCCCEEECC	34.34	24275569
105	Phosphorylation	AYTPPPVSPRFSFPG CCCCCCCCCCCCCCC	19.01	21447557
109	Phosphorylation	PPVSPRFSFPGTTGS CCCCCCCCCCCCHHH	31.63	27732954
113	Phosphorylation	PRFSFPGTTGSLAAA CCCCCCCCHHHHHHH	28.37	27732954
114	Phosphorylation	RFSFPGTTGSLAAAA CCCCCCCHHHHHHHH	30.59	27732954
116	Phosphorylation	SFPGTTGSLAAAAAA CCCCCHHHHHHHHHH	16.85	27732954
132	Phosphorylation	AAREAAAYSSGGGAA HHHHHHHHHCCCCHH	9.91	27732954
133	Phosphorylation	AREAAAYSSGGGAAG HHHHHHHHCCCCHHC	20.13	27732954
133	O-linked_Glycosylation	AREAAAYSSGGGAAG HHHHHHHHCCCCHHC	20.13	30620550
134	Phosphorylation	REAAAYSSGGGAAGA HHHHHHHCCCCHHCC	28.87	27732954
236	Phosphorylation	RRDGTGHYLCNACGL CCCCCCCHHHHHHHH	17.81	17360941
244	Phosphorylation	LCNACGLYHKMNGIN HHHHHHHHHHHCCCC	5.67	17360941
262	Phosphorylation	IKPQRRLSASRRVGL CCHHHHHCHHHHHCC	23.53	22817900
264	Phosphorylation	PQRRLSASRRVGLSC HHHHHCHHHHHCCCC	19.76	-
300	Methylation	NACGLYMKLHGVPRP HHHHHHHHHHCCCCC	24.63	-
312	Acetylation	PRPLAMRKEGIQTRK CCCHHHHHHHHHCCC	47.40	18252717
319	Acetylation	KEGIQTRKRKPKNLN HHHHHCCCCCCCCCC	69.07	18252717
321	Acetylation	GIQTRKRKPKNLNKS HHHCCCCCCCCCCCC	64.48	18252717
323	Acetylation	QTRKRKPKNLNKSKT HCCCCCCCCCCCCCC	76.68	18252717
365	Sumoylation	SEEMRPIKTEPGLSS CHHCCCCCCCCCCCC	49.90	-
395	Phosphorylation	AMSGHGPSIHPVLSA HCCCCCCCHHHHHHH	37.81	-
401	Phosphorylation	PSIHPVLSALKLSPQ CCHHHHHHHHCCCCC	31.61	-
406	Phosphorylation	VLSALKLSPQGYASP HHHHHCCCCCCCCCC	17.37	26657352
410	Phosphorylation	LKLSPQGYASPVSQS HCCCCCCCCCCCCCC	9.92	27732954
412	Phosphorylation	LSPQGYASPVSQSPQ CCCCCCCCCCCCCCC	19.83	27732954
415	Phosphorylation	QGYASPVSQSPQTSS CCCCCCCCCCCCCCC	28.46	27732954
417	Phosphorylation	YASPVSQSPQTSSKQ CCCCCCCCCCCCCCC	16.09	27732954
420	Phosphorylation	PVSQSPQTSSKQDSW CCCCCCCCCCCCCCH	38.05	27732954
421	Phosphorylation	VSQSPQTSSKQDSWN CCCCCCCCCCCCCHH	30.23	27732954
422	Phosphorylation	SQSPQTSSKQDSWNS CCCCCCCCCCCCHHH	37.41	27732954

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
105	S	Phosphorylation	Kinase	CDK4	P11802	PSP
262	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
262	S	Phosphorylation	Kinase	PRKG1	Q13976	GPS
-	K	Ubiquitination	E3 ubiquitin ligase	STUB1	Q9UNE7	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
300	K	Methylation		-
Methylation at Lys-300 attenuates transcriptional activity.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of GATA4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NKX25_HUMAN	NKX2-5	physical	12845333
TBX5_HUMAN	TBX5	physical	12845333
HAND2_HUMAN	HAND2	physical	11994297
FOG2_HUMAN	ZFPM2	physical	9927675
SRF_HUMAN	SRF	physical	11003651
NKX25_HUMAN	NKX2-5	physical	9312027
NKX25_HUMAN	NKX2-5	physical	10948187
ID2_HUMAN	ID2	physical	16556596
ID3_HUMAN	ID3	physical	16556596
ALX4_HUMAN	ALX4	physical	20211142
ZBTB3_HUMAN	ZBTB3	physical	20211142
SP1_HUMAN	SP1	physical	14988427
HDAC2_HUMAN	HDAC2	physical	20833366
EZH2_HUMAN	EZH2	physical	22215809
EZH1_HUMAN	EZH1	physical	22215809
SUZ12_HUMAN	SUZ12	physical	22215809
JARD2_HUMAN	JARID2	physical	22215809
KDM6A_HUMAN	KDM6A	physical	22192413
FOG2_HUMAN	ZFPM2	physical	10801815
JARD2_HUMAN	JARID2	physical	15542826
NKX25_HUMAN	NKX2-5	physical	15542826

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
607941	Atrial septal defect 2 (ASD2)
614429	Ventricular septal defect 1 (VSD1)
187500	Tetralogy of Fallot (TOF)
614430	Atrioventricular septal defect 4 (AVSD4)
615542	Testicular anomalies with or without congenital heart disease (TACHD)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of GATA4_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-406, AND MASSSPECTROMETRY.	19413330 [Abstract]

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