GATA4_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GATA4_RAT
• UniProt AC: P46152
• Protein Name: Transcription factor GATA-4
• Gene Name: Gata4
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 440
• Subcellular Localization: Nucleus .
• Protein Description: Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (By similarity). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (By similarity). Required during testicular development (By similarity). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. [PubMed: 15329343 Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions]
• Protein Sequence: MYQSLAMAANHGPPPGAYEAGGPGAFMHSAGAASSPVYVPTPRVPSSVLGLSYLQGGGSGAASGATSGGSSGAGPSGAGPGTQQGSPGWSQAGAEGAAYTPPPVSPRFSFPGTTGSLAAAAAAAAAREAAAYSSSGGAAGAGLAGREQYGRPGFAGSYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRANPARHPNLDMFDDFSEGRECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAGPPGESLPPSSGASSNSSNATSSSSSSEEMRPIKTEPGLSSHYGHSSSMSQTFSTVSGHGSSIHPVLSALKLSPQGYPSPVTQTSQASSKQDSWNSLVLADSHGDIITA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
105	Phosphorylation	AYTPPPVSPRFSFPG CCCCCCCCCCCCCCC	19.01	22473995
109	Phosphorylation	PPVSPRFSFPGTTGS CCCCCCCCCCCCHHH	31.63	28432305
261	Phosphorylation	IKPQRRLSASRRVGL CCHHHHHCHHHHHCC	23.53	17227882
299	Methylation	NACGLYMKLHGVPRP HHHHHHHHHHCCCCC	24.63	-
404	Phosphorylation	VLSALKLSPQGYPSP HHHHHCCCCCCCCCC	17.37	29779826
408	Phosphorylation	LKLSPQGYPSPVTQT HCCCCCCCCCCCCCC	8.69	28432305
410	Phosphorylation	LSPQGYPSPVTQTSQ CCCCCCCCCCCCCCC	24.42	28432305
413	Phosphorylation	QGYPSPVTQTSQASS CCCCCCCCCCCCCCC	29.52	28432305
415	Phosphorylation	YPSPVTQTSQASSKQ CCCCCCCCCCCCCCC	17.28	28432305
416	Phosphorylation	PSPVTQTSQASSKQD CCCCCCCCCCCCCCC	17.58	28432305
419	Phosphorylation	VTQTSQASSKQDSWN CCCCCCCCCCCCCHH	30.36	28432305
420	Phosphorylation	TQTSQASSKQDSWNS CCCCCCCCCCCCHHH	37.03	28432305
421	Acetylation	QTSQASSKQDSWNSL CCCCCCCCCCCHHHE	56.48	22902405

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
105	S	Phosphorylation	Kinase	ERK2	P28482	PSP
105	S	Phosphorylation	Kinase	MAPK1	P63086	GPS
105	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
261	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
261	S	Phosphorylation	Kinase	PKACA	P27791	PSP
261	S	Phosphorylation	Kinase	RPS6KA3	P51812	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
299	K	Methylation	Methylation at Lys-299 attenuates transcriptional activity.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GATA4_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EP300_HUMAN	EP300	physical	15220332

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.