dbPTM

PINK1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PINK1_HUMAN
UniProt AC	Q9BXM7
Protein Name	Serine/threonine-protein kinase PINK1, mitochondrial
Gene Name	PINK1
Organism	Homo sapiens (Human).
Sequence Length	581
Subcellular Localization	Mitochondrion outer membrane Single-pass membrane protein . Mitochondrion inner membrane Single-pass membrane protein . Cytoplasm, cytosol . Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize
Protein Description	Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN. [PubMed: 14607334]
Protein Sequence	MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
29	Phosphorylation	TGKPGRAYGLGRPGP HCCCCCCCCCCCCCC	16.12	27794612
135	Ubiquitination	IQAIFTQKSKPGPDP HHHHHCCCCCCCCCC	58.34	29967540
137	Ubiquitination	AIFTQKSKPGPDPLD HHHCCCCCCCCCCCC	62.11	29967540
161	Phosphorylation	EEYLIGQSIGKGCSA HHHHHCCCCCCCCCH	28.26	26471730
164	Ubiquitination	LIGQSIGKGCSAAVY HHCCCCCCCCCHHHH	55.89	29967540
186	Ubiquitination	PQNLEVTKSTGLLPG CCCCEEEECCCCCCC	51.72	21963094
198	Phosphorylation	LPGRGPGTSAPGEGQ CCCCCCCCCCCCCCC	25.50	26471730
199	Phosphorylation	PGRGPGTSAPGEGQE CCCCCCCCCCCCCCC	38.08	26471730
228	Phosphorylation	MWNISAGSSSEAILN HHHCCCCCCHHHHHH	30.33	22910362
257	Phosphorylation	AGEYGAVTYRKSKRG CCCCCCCCEECCCCC	19.64	26471730
258	Phosphorylation	GEYGAVTYRKSKRGP CCCCCCCEECCCCCC	14.94	26471730
262	Acetylation	AVTYRKSKRGPKQLA CCCEECCCCCCCCCC	65.49	30591343
284	Phosphorylation	VLRAFTSSVPLLPGA HHHHHHCCCCCCCCC	25.38	-
313	Phosphorylation	EGLGHGRTLFLVMKN CCCCCCCEEEEEEEC	26.84	22238344
319	Ubiquitination	RTLFLVMKNYPCTLR CEEEEEEECCCCCHH	45.94	21963094
402	Phosphorylation	GLQLPFSSWYVDRGG CCCCCCCEEEEECCC	23.86	19229105
433	Ubiquitination	RAVIDYSKADAWAVG CEEEECCHHHHHHHH	44.02	-
495	Phosphorylation	ALLQREASKRPSARV HHHHHHHHCCCCHHH	25.17	-
547	Ubiquitination	LANRLTEKCCVETKM HHHHHHHCHHHHHHH	27.91	29967540

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
228	S	Phosphorylation	Kinase	PRKAA2	P54646	GPS
228	S	Phosphorylation	Kinase	PINK1	Q9BXM7	PSP
257	T	Phosphorylation	Kinase	PINK1	Q9BXM7	PSP
284	S	Phosphorylation	Kinase	PRKAA2	P54646	GPS
313	T	Phosphorylation	Kinase	MARK2	Q7KZI7	PSP
402	S	Phosphorylation	Kinase	PINK1	Q9BXM7	PSP
495	S	Phosphorylation	Kinase	PRKAA2	P54646	GPS
-	K	Ubiquitination	E3 ubiquitin ligase	PRKN	O60260	PMID:22724072
-	K	Ubiquitination	E3 ubiquitin ligase	TRAF6	Q9Y4K3	PMID:23885119

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
228	S	Phosphorylation	22910362
Autophosphorylation at Ser-228 and Ser-402 is sufficient and essential for selective recruitment of PRKN to depolarized mitochondria, via PINK1-dependent phosphorylation of ubiquitin and maybe PRKN.
228	S	ubiquitylation	22910362
Autophosphorylation at Ser-228 and Ser-402 is sufficient and essential for selective recruitment of PRKN to depolarized mitochondria, via PINK1-dependent phosphorylation of ubiquitin and maybe PRKN.
402	S	Phosphorylation	22910362
Autophosphorylation at Ser-228 and Ser-402 is sufficient and essential for selective recruitment of PRKN to depolarized mitochondria, via PINK1-dependent phosphorylation of ubiquitin and maybe PRKN.
402	S	ubiquitylation	22910362
Autophosphorylation at Ser-228 and Ser-402 is sufficient and essential for selective recruitment of PRKN to depolarized mitochondria, via PINK1-dependent phosphorylation of ubiquitin and maybe PRKN.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PINK1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PRKN_HUMAN	PARK2	physical	19358826
PINK1_HUMAN	PINK1	physical	14607334
MAP1B_HUMAN	MAP1B	physical	21151955
PRKDC_HUMAN	PRKDC	physical	21151955
IRS4_HUMAN	IRS4	physical	21151955
KIF11_HUMAN	KIF11	physical	21151955
PSMD1_HUMAN	PSMD1	physical	21151955
PSMD2_HUMAN	PSMD2	physical	21151955
HS90A_HUMAN	HSP90AA1	physical	21151955
HSP74_HUMAN	HSPA4	physical	21151955
TBA1A_HUMAN	TUBA1A	physical	21151955
TBB5_HUMAN	TUBB	physical	21151955
CDC37_HUMAN	CDC37	physical	21151955
PRS10_HUMAN	PSMC6	physical	21151955
PGAM5_HUMAN	PGAM5	physical	21151955
PARL_HUMAN	PARL	physical	21355049
MLP3A_HUMAN	MAP1LC3A	physical	20153330
HS90A_HUMAN	HSP90AA1	physical	18003639
CDC37_HUMAN	CDC37	physical	18003639
HS90B_HUMAN	HSP90AB1	physical	18003639
SYUA_HUMAN	SNCA	physical	19167501
HS90A_HUMAN	HSP90AA1	physical	18359116
HSP74_HUMAN	HSPA4	physical	18359116
CDC37_HUMAN	CDC37	physical	18359116
PARK7_HUMAN	PARK7	physical	16632486
HTRA2_HUMAN	HTRA2	physical	17906618
PINK1_HUMAN	PINK1	physical	22280891
TOM40_HUMAN	TOMM40	physical	22280891
TOM70_HUMAN	TOMM70A	physical	22280891
TOM22_HUMAN	TOMM22	physical	22280891
TOM20_HUMAN	TOMM20	physical	22280891
PRKN_HUMAN	PARK2	physical	19229105
PARK7_HUMAN	PARK7	physical	19229105
PRKN_HUMAN	PARK2	physical	22724072
PINK1_HUMAN	PINK1	physical	22724072
PRKN_HUMAN	PARK2	physical	19880420
RICTR_HUMAN	RICTOR	physical	21177249
SIN1_HUMAN	MAPKAP1	physical	21177249
AKT1_HUMAN	AKT1	physical	21177249
UBC12_HUMAN	UBE2M	physical	22388932
CSN8_HUMAN	COPS8	physical	22388932
TRAF6_HUMAN	TRAF6	physical	22643835
M3K7_HUMAN	MAP3K7	physical	22643835
TOLIP_HUMAN	TOLLIP	physical	23244239
IRAK1_HUMAN	IRAK1	physical	23244239
VIME_HUMAN	VIM	physical	23885119
SARM1_HUMAN	SARM1	physical	23885119
RBCC1_HUMAN	RB1CC1	physical	23885119
BAG2_HUMAN	BAG2	physical	24513209
CRLS1_HUMAN	CRLS1	physical	24703837
UBC_HUMAN	UBC	physical	24784582
UBC_HUMAN	UBC	physical	24751536
MIRO2_HUMAN	RHOT2	physical	19152501
MIC60_HUMAN	IMMT	physical	19152501
ULA1_HUMAN	NAE1	physical	22388932
PRKN_HUMAN	PARK2	physical	24357652
PRKN_HUMAN	PARK2	physical	19966284
TGM2_HUMAN	TGM2	physical	25557247
PRKN_HUMAN	PARK2	physical	25474007
UBC_HUMAN	UBC	physical	25474007
PRKN_HUMAN	PARK2	physical	25591737
HCD2_HUMAN	HSD17B10	physical	25591737
DNM1L_HUMAN	DNM1L	physical	25591737
UBC_HUMAN	UBC	physical	25847540
PRKN_HUMAN	PARK2	physical	26162776
UBC_HUMAN	UBC	physical	26162776
PRKN_HUMAN	PARK2	physical	26116755
PRKN_HUMAN	PARK2	physical	17052189
BAG5_HUMAN	BAG5	physical	24475098
SYUA_HUMAN	SNCA	physical	27334109
BNIP3_HUMAN	BNIP3	physical	27528605
HS90A_HUMAN	HSP90AA1	physical	28438176
CDC37_HUMAN	CDC37	physical	28438176
TOM40_HUMAN	TOMM40	physical	28438176
UBC_HUMAN	UBC	physical	28438176

Drug and Disease Associations

Kegg Disease
H00057	Parkinson's disease (PD)
OMIM Disease
605909	Parkinson disease 6 (PARK6)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PINK1_HUMAN

Related Literatures of Post-Translational Modification

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