dbPTM

BNIP3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	BNIP3_HUMAN
UniProt AC	Q12983
Protein Name	BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Gene Name	BNIP3
Organism	Homo sapiens (Human).
Sequence Length	259
Subcellular Localization	Mitochondrion. Mitochondrion outer membrane Single-pass membrane protein. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with
Protein Description	Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway..
Protein Sequence	MGDAAADPPGPALPCEFLRPGCGAPLSPGAQLGRGAPTSAFPPPAAEAHPAARRGLRSPQLPSGAMSQNGAPGMQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRASETDTHSIGEKNSSQSEEDDIERRKEVESILKKNSDWIWDWSSRPENIPPKEFLFKHPKRTATLSMRNTSVMKKGGIFSAEFLKVFLPSLLLSHLLAIGLGIYIGRRLTTSTSTF

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
17	Phosphorylation	GPALPCEFLRPGCGA CCCCCCCCCCCCCCC	18.26	-
24	Phosphorylation	FLRPGCGAPLSPGAQ CCCCCCCCCCCCCCC	20.73	-
39	Phosphorylation	LGRGAPTSAFPPPAA CCCCCCCCCCCCCHH	12.94	-
54	Phosphorylation	EAHPAARRGLRSPQL HHCHHHHCCCCCCCC	37.06	24961811
57	Phosphorylation	PAARRGLRSPQLPSG HHHHCCCCCCCCCCC	42.35	25627689
58	Phosphorylation	AARRGLRSPQLPSGA HHHCCCCCCCCCCCC	40.19	24961811
59	Phosphorylation	ARRGLRSPQLPSGAM HHCCCCCCCCCCCCC	37.49	25627689
61	Phosphorylation	RGLRSPQLPSGAMSQ CCCCCCCCCCCCCCC	27.54	25262027
62	Phosphorylation	GLRSPQLPSGAMSQN CCCCCCCCCCCCCCC	31.69	-
66	Phosphorylation	PQLPSGAMSQNGAPG CCCCCCCCCCCCCCC	42.04	22798277
70	Phosphorylation	SGAMSQNGAPGMQEE CCCCCCCCCCCCCCH	44.15	25627689
72	Phosphorylation	AMSQNGAPGMQEESL CCCCCCCCCCCCHHH	24.94	22798277
82	Phosphorylation	QEESLQGSWVELHFS CCHHHCCCEEEEEEE	43.64	-
84	Phosphorylation	ESLQGSWVELHFSNN HHHCCCEEEEEEECC	25.58	25262027
86	Phosphorylation	LQGSWVELHFSNNGN HCCCEEEEEEECCCC	34.09	20363803
89	Phosphorylation	SWVELHFSNNGNGGS CEEEEEEECCCCCCC	47.60	-
90	Ubiquitination	WVELHFSNNGNGGSV EEEEEEECCCCCCCC	57.84	21906983
92	Phosphorylation	ELHFSNNGNGGSVPA EEEEECCCCCCCCCE	40.60	20363803
93	Phosphorylation	LHFSNNGNGGSVPAS EEEECCCCCCCCCEE	47.31	20363803
95	Phosphorylation	FSNNGNGGSVPASVS EECCCCCCCCCEEEE	44.92	20363803
104	Ubiquitination	VPASVSIYNGDMEKI CCEEEEEECCCHHEE	72.79	-
104	Phosphorylation	VPASVSIYNGDMEKI CCEEEEEECCCHHEE	72.79	25884760
111	Ubiquitination	YNGDMEKILLDAQHE ECCCHHEEEHHHHHH	48.52	-
119	Phosphorylation	LLDAQHESGRSSSKS EHHHHHHCCCCCCCC	26.67	25849741
122	Phosphorylation	AQHESGRSSSKSSHC HHHHCCCCCCCCCCC	47.83	25849741
123	Phosphorylation	QHESGRSSSKSSHCD HHHCCCCCCCCCCCC	36.72	29514088
124	Phosphorylation	HESGRSSSKSSHCDS HHCCCCCCCCCCCCC	40.72	25159151
126	Phosphorylation	SGRSSSKSSHCDSPP CCCCCCCCCCCCCCC	55.54	28450419
127	Phosphorylation	GRSSSKSSHCDSPPR CCCCCCCCCCCCCCC	6.19	28450419
131	Phosphorylation	SKSSHCDSPPRSQTP CCCCCCCCCCCCCCC	54.05	25159151
135	Ubiquitination	HCDSPPRSQTPQDTN CCCCCCCCCCCCCCC	62.23	-
135	Phosphorylation	HCDSPPRSQTPQDTN CCCCCCCCCCCCCCC	62.23	25262027
137	Phosphorylation	DSPPRSQTPQDTNRA CCCCCCCCCCCCCCC	28.54	25159151
139	Methylation	PPRSQTPQDTNRASE CCCCCCCCCCCCCCC	33.76	-
140	Phosphorylation	PRSQTPQDTNRASET CCCCCCCCCCCCCCC	30.07	20068231
141	Phosphorylation	RSQTPQDTNRASETD CCCCCCCCCCCCCCC	15.58	30108239
142	Phosphorylation	SQTPQDTNRASETDT CCCCCCCCCCCCCCC	20.82	20068231
144	Phosphorylation	TPQDTNRASETDTHS CCCCCCCCCCCCCCC	16.02	-
145	Phosphorylation	PQDTNRASETDTHSI CCCCCCCCCCCCCCC	4.27	23403867
147	Phosphorylation	DTNRASETDTHSIGE CCCCCCCCCCCCCCC	28.23	23403867
148	Phosphorylation	TNRASETDTHSIGEK CCCCCCCCCCCCCCC	16.60	20068231
149	Phosphorylation	NRASETDTHSIGEKN CCCCCCCCCCCCCCC	24.62	23927012
151	Phosphorylation	ASETDTHSIGEKNSS CCCCCCCCCCCCCCC	3.58	29255136
152	Ubiquitination	SETDTHSIGEKNSSQ CCCCCCCCCCCCCCC	46.22	-
153	Ubiquitination	ETDTHSIGEKNSSQS CCCCCCCCCCCCCCC	44.25	-
155	Ubiquitination	DTHSIGEKNSSQSEE CCCCCCCCCCCCCCH	27.01	-
157	Phosphorylation	HSIGEKNSSQSEEDD CCCCCCCCCCCCHHH	6.82	25159151
158	Phosphorylation	SIGEKNSSQSEEDDI CCCCCCCCCCCHHHH	25.60	25159151
160	Phosphorylation	GEKNSSQSEEDDIER CCCCCCCCCHHHHHH	50.76	28355574
169	Ubiquitination	EDDIERRKEVESILK HHHHHHHHHHHHHHH	5.42	-
173	Phosphorylation	ERRKEVESILKKNSD HHHHHHHHHHHHCCC	25.49	29514088
176	Ubiquitination	KEVESILKKNSDWIW HHHHHHHHHCCCCCC	10.52	-
188	Phosphorylation	WIWDWSSRPENIPPK CCCCCCCCCCCCCCH	19.52	-
189	Phosphorylation	IWDWSSRPENIPPKE CCCCCCCCCCCCCHH	34.30	-
190	Phosphorylation	WDWSSRPENIPPKEF CCCCCCCCCCCCHHH	18.35	-
191	Phosphorylation	DWSSRPENIPPKEFL CCCCCCCCCCCHHHH	29.69	-
192	Phosphorylation	WSSRPENIPPKEFLF CCCCCCCCCCHHHHC	27.81	-
193	Phosphorylation	SSRPENIPPKEFLFK CCCCCCCCCHHHHCC	33.19	-
195	Ubiquitination	RPENIPPKEFLFKHP CCCCCCCHHHHCCCC		-
200	Ubiquitination	PPKEFLFKHPKRTAT CCHHHHCCCCCCEEE		-
204	Methylation	FLFKHPKRTATLSMR HHCCCCCCEEEEEEC		-
205	Phosphorylation	LFKHPKRTATLSMRN HCCCCCCEEEEEECC		20068231
207	Phosphorylation	KHPKRTATLSMRNTS CCCCCEEEEEECCCH		24275569
209	Phosphorylation	PKRTATLSMRNTSVM CCCEEEEEECCCHHH		20068231
213	Phosphorylation	ATLSMRNTSVMKKGG EEEEECCCHHHHCCC		20068231
214	Phosphorylation	TLSMRNTSVMKKGGI EEEECCCHHHHCCCC		20068231
218	Ubiquitination	RNTSVMKKGGIFSAE CCCHHHHCCCCCCHH		-
253	Phosphorylation	IYIGRRLTTSTSTF- HHHCCCCCCCCCCC-		-
254	Phosphorylation	YIGRRLTTSTSTF-- HHCCCCCCCCCCC--		-
255	Phosphorylation	IGRRLTTSTSTF--- HCCCCCCCCCCC---		-
256	Phosphorylation	GRRLTTSTSTF---- CCCCCCCCCCC----		-
257	Phosphorylation	RRLTTSTSTF----- CCCCCCCCCC-----		-
258	Phosphorylation	RLTTSTSTF------ CCCCCCCCC------		-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
253	T	Phosphorylation	Kinase	PKACA	P17612	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of BNIP3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of BNIP3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TMM11_HUMAN	TMEM11	physical	16189514
BNI3L_HUMAN	BNIP3L	physical	16189514
BNIP3_HUMAN	BNIP3	physical	16189514
BNIP3_HUMAN	BNIP3	physical	9396766
B2CL1_HUMAN	BCL2L1	physical	10625696
BNIP3_HUMAN	BNIP3	physical	10625696
HDAC1_HUMAN	HDAC1	physical	19339613
RHEB_HUMAN	RHEB	physical	17928295
TGM2_HUMAN	TGM2	physical	21988832
ZEP1_HUMAN	HIVEP1	physical	21988832
BCL2_HUMAN	BCL2	physical	23504944
BNIP3_HUMAN	BNIP3	physical	25416956
BNI3L_HUMAN	BNIP3L	physical	25416956
KTN1_HUMAN	KTN1	physical	25416956
TMM11_HUMAN	TMEM11	physical	25416956
CLC7A_HUMAN	CLEC7A	physical	25416956
FATE1_HUMAN	FATE1	physical	25416956
LRAD1_HUMAN	LDLRAD1	physical	25416956
OPA1_HUMAN	OPA1	physical	20436456
BNI3L_HUMAN	BNIP3L	physical	21516116
BNI3L_HUMAN	BNIP3L	physical	28514442
PPTC7_HUMAN	PPTC7	physical	28514442
TMM11_HUMAN	TMEM11	physical	28514442
P4R3B_HUMAN	SMEK2	physical	28514442
PINK1_HUMAN	PINK1	physical	27528605

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of BNIP3_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Evaluation of the low-specificity protease elastase for large-scalephosphoproteome analysis."; Wang B., Malik R., Nigg E.A., Korner R.; Anal. Chem. 80:9526-9533(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93, AND MASSSPECTROMETRY.	19007248 [Abstract]