ULA1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ULA1_HUMAN
• UniProt AC: Q13564
• Protein Name: NEDD8-activating enzyme E1 regulatory subunit
• Gene Name: NAE1
• Organism: Homo sapiens (Human).
• Sequence Length: 534
• Subcellular Localization: Cell membrane . Colocalizes with APP in lipid rafts.
• Protein Description: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation..
• Protein Sequence: MAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAQLGKLLK ------CHHHHHHHH	17.19	22814378
6	Acetylation	--MAQLGKLLKEQKY --CHHHHHHHHHCCC	60.27	19608861
6	Ubiquitination	--MAQLGKLLKEQKY --CHHHHHHHHHCCC	60.27	23000965
6	Acetylation	--MAQLGKLLKEQKY --CHHHHHHHHHCCC	60.27	-
6	Ubiquitination	--MAQLGKLLKEQKY --CHHHHHHHHHCCC	60.27	-
7 (in isoform 2)	Ubiquitination	-	8.62	-
9	Ubiquitination	AQLGKLLKEQKYDRQ HHHHHHHHHCCCHHH	69.41	23000965
9	Ubiquitination	AQLGKLLKEQKYDRQ HHHHHHHHHCCCHHH	69.41	-
12	Ubiquitination	GKLLKEQKYDRQLRL HHHHHHCCCHHHHHC	50.65	23000965
12	Ubiquitination	GKLLKEQKYDRQLRL HHHHHHCCCHHHHHC	50.65	-
13	Phosphorylation	KLLKEQKYDRQLRLW HHHHHCCCHHHHHCC	18.95	-
78	Ubiquitination	GNNFFLQRSSIGKNR CCCCEEEHHHCCCCH	34.22	21890473
78	Ubiquitination	GNNFFLQRSSIGKNR CCCCEEEHHHCCCCH	34.22	21890473
98	Ubiquitination	EFLQELNSDVSGSFV HHHHHHCCCCCCCCC	52.60	21890473
98	Ubiquitination	EFLQELNSDVSGSFV HHHHHHCCCCCCCCC	52.60	21890473
117	Ubiquitination	ENLLDNDPSFFCRFT CCCCCCCCCCCCEEE	39.01	33845483
118	Ubiquitination	NLLDNDPSFFCRFTV CCCCCCCCCCCEEEE	33.54	33845483
124	Phosphorylation	PSFFCRFTVVVATQL CCCCCEEEEEEEECC	7.95	21406692
129	Phosphorylation	RFTVVVATQLPESTS EEEEEEEECCCCCCC	21.01	21406692
134	Phosphorylation	VATQLPESTSLRLAD EEECCCCCCCHHHHH	22.74	21406692
135	Phosphorylation	ATQLPESTSLRLADV EECCCCCCCHHHHHH	30.07	21406692
136	Phosphorylation	TQLPESTSLRLADVL ECCCCCCCHHHHHHH	22.29	21406692
151	Ubiquitination	WNSQIPLLICRTYGL HHCCCCHHHHHHHCH	2.66	29967540
161	Ubiquitination	RTYGLVGYMRIIIKE HHHCHHHEEEEEECC	3.92	21890473
161 (in isoform 2)	Ubiquitination	-	3.92	-
161	Ubiquitination	RTYGLVGYMRIIIKE HHHCHHHEEEEEECC	3.92	21890473
164	Ubiquitination	GLVGYMRIIIKEHPV CHHHEEEEEECCCCC	1.86	23000965
164	Neddylation	GLVGYMRIIIKEHPV CHHHEEEEEECCCCC	1.86	32015554
164	Ubiquitination	GLVGYMRIIIKEHPV CHHHEEEEEECCCCC	1.86	21890473
167	Ubiquitination	GYMRIIIKEHPVIES HEEEEEECCCCCCCC	39.29	21906983
170	Ubiquitination	RIIIKEHPVIESHPD EEEECCCCCCCCCCC	29.85	21890473
170	Ubiquitination	RIIIKEHPVIESHPD EEEECCCCCCCCCCC	29.85	21890473
181	Ubiquitination	SHPDNALEDLRLDKP CCCCCHHHHHCCCCC	53.22	23000965
181	Neddylation	SHPDNALEDLRLDKP CCCCCHHHHHCCCCC	53.22	32015554
181 (in isoform 2)	Ubiquitination	-	53.22	-
181	Ubiquitination	SHPDNALEDLRLDKP CCCCCHHHHHCCCCC	53.22	21890473
187	Ubiquitination	LEDLRLDKPFPELRE HHHHCCCCCCHHHHH	53.82	23000965
190	Ubiquitination	LRLDKPFPELREHFQ HCCCCCCHHHHHHHH	48.52	21890473
190	Ubiquitination	LRLDKPFPELREHFQ HCCCCCCHHHHHHHH	48.52	21890473
200	Ubiquitination	REHFQSYDLDHMEKK HHHHHHCCCHHHHHC	51.36	33845483
200 (in isoform 2)	Ubiquitination	-	51.36	-
201	Ubiquitination	EHFQSYDLDHMEKKD HHHHHCCCHHHHHCC	3.56	33845483
201 (in isoform 2)	Ubiquitination	-	3.56	-
204	Sulfoxidation	QSYDLDHMEKKDHSH HHCCCHHHHHCCCCC	8.90	30846556
206	Ubiquitination	YDLDHMEKKDHSHTP CCCHHHHHCCCCCCC	56.79	33845483
207	Ubiquitination	DLDHMEKKDHSHTPW CCHHHHHCCCCCCCH	47.57	33845483
209	Ubiquitination	DHMEKKDHSHTPWIV HHHHHCCCCCCCHHH	30.29	33845483
209	Ubiquitination	DHMEKKDHSHTPWIV HHHHHCCCCCCCHHH	30.29	-
210	Ubiquitination	HMEKKDHSHTPWIVI HHHHCCCCCCCHHHH	38.91	21963094
210	Ubiquitination	HMEKKDHSHTPWIVI HHHHCCCCCCCHHHH	38.91	-
210	Ubiquitination	HMEKKDHSHTPWIVI HHHHCCCCCCCHHHH	38.91	21890473
223	Ubiquitination	VIIAKYLAQWYSETN HHHHHHHHHHHHHHC	8.51	23000965
223	Ubiquitination	VIIAKYLAQWYSETN HHHHHHHHHHHHHHC	8.51	21890473
226	Phosphorylation	AKYLAQWYSETNGRI HHHHHHHHHHHCCCC	5.64	18083107
228	Ubiquitination	YLAQWYSETNGRIPK HHHHHHHHHCCCCCC	30.51	23000965
228	Ubiquitination	YLAQWYSETNGRIPK HHHHHHHHHCCCCCC	30.51	21890473
229 (in isoform 2)	Ubiquitination	-	36.71	-
232 (in isoform 2)	Ubiquitination	-	44.07	-
234	Ubiquitination	SETNGRIPKTYKEKE HHHCCCCCCCHHCHH	22.90	29967540
234 (in isoform 2)	Ubiquitination	-	22.90	-
237	Phosphorylation	NGRIPKTYKEKEDFR CCCCCCCHHCHHHHH	24.23	18083107
238	Ubiquitination	GRIPKTYKEKEDFRD CCCCCCHHCHHHHHH	68.49	-
238	Ubiquitination	GRIPKTYKEKEDFRD CCCCCCHHCHHHHHH	68.49	-
240	Ubiquitination	IPKTYKEKEDFRDLI CCCCHHCHHHHHHHH	59.43	29967540
241	Ubiquitination	PKTYKEKEDFRDLIR CCCHHCHHHHHHHHH	64.57	-
243	Ubiquitination	TYKEKEDFRDLIRQG CHHCHHHHHHHHHHH	7.40	29967540
243	Ubiquitination	TYKEKEDFRDLIRQG CHHCHHHHHHHHHHH	7.40	-
247	Ubiquitination	KEDFRDLIRQGILKN HHHHHHHHHHHHHHC	3.46	23000965
247	Neddylation	KEDFRDLIRQGILKN HHHHHHHHHHHHHHC	3.46	32015554
247 (in isoform 2)	Ubiquitination	-	3.46	-
249	Ubiquitination	DFRDLIRQGILKNEN HHHHHHHHHHHHCCC	35.96	23000965
249	Ubiquitination	DFRDLIRQGILKNEN HHHHHHHHHHHHCCC	35.96	21890473
252	Ubiquitination	DLIRQGILKNENGAP HHHHHHHHHCCCCCC	6.61	23000965
252	Ubiquitination	DLIRQGILKNENGAP HHHHHHHHHCCCCCC	6.61	21890473
253	Ubiquitination	LIRQGILKNENGAPE HHHHHHHHCCCCCCC	61.36	23000965
253	Sumoylation	LIRQGILKNENGAPE HHHHHHHHCCCCCCC	61.36	-
253	Neddylation	LIRQGILKNENGAPE HHHHHHHHCCCCCCC	61.36	32015554
256	Ubiquitination	QGILKNENGAPEDEE HHHHHCCCCCCCCHH	62.41	23000965
256	Neddylation	QGILKNENGAPEDEE HHHHHCCCCCCCCHH	62.41	32015554
256	Ubiquitination	QGILKNENGAPEDEE HHHHHCCCCCCCCHH	62.41	-
260	Ubiquitination	KNENGAPEDEENFEE HCCCCCCCCHHHHHH	77.00	29967540
263	Ubiquitination	NGAPEDEENFEEAIK CCCCCCHHHHHHHHH	78.51	33845483
264	Ubiquitination	GAPEDEENFEEAIKN CCCCCHHHHHHHHHH	47.81	32015554
264	Neddylation	GAPEDEENFEEAIKN CCCCCHHHHHHHHHH	47.81	32015554
270	Ubiquitination	ENFEEAIKNVNTALN HHHHHHHHHHHHHHH	62.99	32015554
270	Neddylation	ENFEEAIKNVNTALN HHHHHHHHHHHHHHH	62.99	32015554
273	Ubiquitination	EEAIKNVNTALNTTQ HHHHHHHHHHHHCCC	29.09	32015554
273	Neddylation	EEAIKNVNTALNTTQ HHHHHHHHHHHHCCC	29.09	32015554
274	Ubiquitination	EAIKNVNTALNTTQI HHHHHHHHHHHCCCC	28.39	23000965
274	Ubiquitination	EAIKNVNTALNTTQI HHHHHHHHHHHCCCC	28.39	21890473
283	Phosphorylation	LNTTQIPSSIEDIFN HHCCCCCCCHHHHHC	45.65	25693802
284	Phosphorylation	NTTQIPSSIEDIFND HCCCCCCCHHHHHCC	25.33	25693802
289	Ubiquitination	PSSIEDIFNDDRCIN CCCHHHHHCCCCEEE	14.70	21963094
289	Ubiquitination	PSSIEDIFNDDRCIN CCCHHHHHCCCCEEE	14.70	21890473
292	Ubiquitination	IEDIFNDDRCINITK HHHHHCCCCEEECCC	48.75	22817900
293	Ubiquitination	EDIFNDDRCINITKQ HHHHCCCCEEECCCC	26.53	21963094
293 (in isoform 2)	Ubiquitination	-	26.53	-
293	Ubiquitination	EDIFNDDRCINITKQ HHHHCCCCEEECCCC	26.53	21890473
294	Glutathionylation	DIFNDDRCINITKQT HHHCCCCEEECCCCC	3.43	22555962
299	Ubiquitination	DRCINITKQTPSFWI CCEEECCCCCHHHHH	48.67	21963094
302	Ubiquitination	INITKQTPSFWILAR EECCCCCHHHHHHHH	25.65	21963094
302	Ubiquitination	INITKQTPSFWILAR EECCCCCHHHHHHHH	25.65	21890473
306	Ubiquitination	KQTPSFWILARALKE CCCHHHHHHHHHHHH	1.71	23000965
306 (in isoform 2)	Ubiquitination	-	1.71	-
306	Ubiquitination	KQTPSFWILARALKE CCCHHHHHHHHHHHH	1.71	21890473
311	Ubiquitination	FWILARALKEFVAKE HHHHHHHHHHHHHHH	4.56	23000965
311 (in isoform 2)	Ubiquitination	-	4.56	-
311	Ubiquitination	FWILARALKEFVAKE HHHHHHHHHHHHHHH	4.56	21890473
312	Ubiquitination	WILARALKEFVAKEG HHHHHHHHHHHHHHC	48.39	23000965
315	Ubiquitination	ARALKEFVAKEGQGN HHHHHHHHHHHCCCC	8.57	23000965
315	Ubiquitination	ARALKEFVAKEGQGN HHHHHHHHHHHCCCC	8.57	21890473
317	Ubiquitination	ALKEFVAKEGQGNLP HHHHHHHHHCCCCCC	58.01	23000965
320	Ubiquitination	EFVAKEGQGNLPVRG HHHHHHCCCCCCCCC	38.40	23000965
320	Ubiquitination	EFVAKEGQGNLPVRG HHHHHHCCCCCCCCC	38.40	21890473
332	Ubiquitination	VRGTIPDMIADSGKY CCCCCCHHHHCCCCE	1.98	23000965
332 (in isoform 2)	Ubiquitination	-	1.98	-
332	Ubiquitination	VRGTIPDMIADSGKY CCCCCCHHHHCCCCE	1.98	21890473
335	Ubiquitination	TIPDMIADSGKYIKL CCCHHHHCCCCEEEE	47.18	23000965
335 (in isoform 2)	Ubiquitination	-	47.18	-
335	Ubiquitination	TIPDMIADSGKYIKL CCCHHHHCCCCEEEE	47.18	21890473
336	Phosphorylation	IPDMIADSGKYIKLQ CCHHHHCCCCEEEEH	28.32	23532336
338	Acetylation	DMIADSGKYIKLQNV HHHHCCCCEEEEHHH	47.99	23954790
338	Ubiquitination	DMIADSGKYIKLQNV HHHHCCCCEEEEHHH	47.99	23000965
339	Phosphorylation	MIADSGKYIKLQNVY HHHCCCCEEEEHHHH	13.55	-
341	Acetylation	ADSGKYIKLQNVYRE HCCCCEEEEHHHHHH	40.71	23954790
341	Ubiquitination	ADSGKYIKLQNVYRE HCCCCEEEEHHHHHH	40.71	23000965
341	Acetylation	ADSGKYIKLQNVYRE HCCCCEEEEHHHHHH	40.71	-
341	Ubiquitination	ADSGKYIKLQNVYRE HCCCCEEEEHHHHHH	40.71	21890473
343	Ubiquitination	SGKYIKLQNVYREKA CCCEEEEHHHHHHHH	32.13	29967540
344	Ubiquitination	GKYIKLQNVYREKAK CCEEEEHHHHHHHHH	43.13	23000965
344	Acetylation	GKYIKLQNVYREKAK CCEEEEHHHHHHHHH	43.13	-
344	Ubiquitination	GKYIKLQNVYREKAK CCEEEEHHHHHHHHH	43.13	21890473
346	Phosphorylation	YIKLQNVYREKAKKD EEEEHHHHHHHHHHH	22.17	-
346	Ubiquitination	YIKLQNVYREKAKKD EEEEHHHHHHHHHHH	22.17	33845483
349	Ubiquitination	LQNVYREKAKKDAAA EHHHHHHHHHHHHHH	57.41	29967540
352	Ubiquitination	VYREKAKKDAAAVGN HHHHHHHHHHHHHHH	58.41	33845483
355	Ubiquitination	EKAKKDAAAVGNHVA HHHHHHHHHHHHHHH	16.89	33845483
357	Ubiquitination	AKKDAAAVGNHVAKL HHHHHHHHHHHHHHH	7.27	23000965
357 (in isoform 2)	Ubiquitination	-	7.27	-
357	Ubiquitination	AKKDAAAVGNHVAKL HHHHHHHHHHHHHHH	7.27	21890473
363	Ubiquitination	AVGNHVAKLLQSIGQ HHHHHHHHHHHHHCC	48.95	23000965
366	Ubiquitination	NHVAKLLQSIGQAPE HHHHHHHHHHCCCCC	43.36	23000965
366	Ubiquitination	NHVAKLLQSIGQAPE HHHHHHHHHHCCCCC	43.36	21890473
367	Ubiquitination	HVAKLLQSIGQAPES HHHHHHHHHCCCCCC	28.76	21963094
367	Ubiquitination	HVAKLLQSIGQAPES HHHHHHHHHCCCCCC	28.76	21890473
372	Ubiquitination	LQSIGQAPESISEKE HHHHCCCCCCCCHHH	28.16	21963094
372 (in isoform 2)	Ubiquitination	-	28.16	-
372	Ubiquitination	LQSIGQAPESISEKE HHHHCCCCCCCCHHH	28.16	21890473
374	Phosphorylation	SIGQAPESISEKELK HHCCCCCCCCHHHHH	30.29	20860994
375	Ubiquitination	IGQAPESISEKELKL HCCCCCCCCHHHHHH	6.17	22817900
375 (in isoform 2)	Ubiquitination	-	6.17	-
376	Phosphorylation	GQAPESISEKELKLL CCCCCCCCHHHHHHH	52.47	20860994
378	Acetylation	APESISEKELKLLCS CCCCCCHHHHHHHHC	62.65	23954790
378	Ubiquitination	APESISEKELKLLCS CCCCCCHHHHHHHHC	62.65	21906983
381	Ubiquitination	SISEKELKLLCSNSA CCCHHHHHHHHCCCH	40.27	21963094
381	Acetylation	SISEKELKLLCSNSA CCCHHHHHHHHCCCH	40.27	-
381	Ubiquitination	SISEKELKLLCSNSA CCCHHHHHHHHCCCH	40.27	21890473
381	Acetylation	SISEKELKLLCSNSA CCCHHHHHHHHCCCH	40.27	25953088
384	Ubiquitination	EKELKLLCSNSAFLR HHHHHHHHCCCHHHH	5.40	22817900
384	Ubiquitination	EKELKLLCSNSAFLR HHHHHHHHCCCHHHH	5.40	-
384	Glutathionylation	EKELKLLCSNSAFLR HHHHHHHHCCCHHHH	5.40	22555962
397	Phosphorylation	LRVVRCRSLAEEYGL HHHHHHHHHHHHHCC	35.24	19845377
406	Phosphorylation	AEEYGLDTINKDEII HHHHCCCCCCHHHHH	31.34	19845377
442	Phosphorylation	FHKQQGRYPGVSNYQ HHHHCCCCCCCCCCC	15.91	29396449
446	Phosphorylation	QGRYPGVSNYQVEED CCCCCCCCCCCHHCH	35.42	29396449
448	Phosphorylation	RYPGVSNYQVEEDIG CCCCCCCCCHHCHHH	13.36	29396449
450	Ubiquitination	PGVSNYQVEEDIGKL CCCCCCCHHCHHHHH	6.49	21963094
450 (in isoform 2)	Ubiquitination	-	6.49	-
456	Ubiquitination	QVEEDIGKLKSCLTG CHHCHHHHHHHHHHH	53.74	21906983
459	Ubiquitination	EDIGKLKSCLTGFLQ CHHHHHHHHHHHHHH	25.46	21963094
459	Ubiquitination	EDIGKLKSCLTGFLQ CHHHHHHHHHHHHHH	25.46	-
501 (in isoform 2)	Ubiquitination	-	16.98	-
507	Ubiquitination	AAAQEVIKIITKQFV HHHHHHHHHHHCCEE	32.98	-
510	Ubiquitination	QEVIKIITKQFVIFN HHHHHHHHCCEEEEC	23.43	-
522	Phosphorylation	IFNNTYIYSGMSQTS EECCEEEEECCCCCE	6.69	25022875

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	TRIP12	Q14669	PMID:18627766

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ULA1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ULA1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
A4_HUMAN	APP	physical	8626687
UBA3_HUMAN	UBA3	physical	10722740
UBA3_HUMAN	UBA3	physical	18264111
NEDD8_HUMAN	NEDD8	physical	18264111
UBA3_HUMAN	UBA3	physical	17220875
NEDD8_HUMAN	NEDD8	physical	17220875
UBC12_HUMAN	UBE2M	physical	17220875
UBA3_HUMAN	UBA3	physical	12646924
UBA3_HUMAN	UBA3	physical	12740388
UBC12_HUMAN	UBE2M	physical	18652489
UBA3_HUMAN	UBA3	physical	9694792
SRBP2_HUMAN	SREBF2	physical	21988832
APEX1_HUMAN	APEX1	physical	22863883
CAN2_HUMAN	CAPN2	physical	22863883
DCPS_HUMAN	DCPS	physical	22863883
MCTS1_HUMAN	MCTS1	physical	22863883
MOES_HUMAN	MSN	physical	22863883
NSF1C_HUMAN	NSFL1C	physical	22863883
PAPS1_HUMAN	PAPSS1	physical	22863883
PCY2_HUMAN	PCYT2	physical	22863883
IPP2_HUMAN	PPP1R2	physical	22863883
TRXR1_HUMAN	TXNRD1	physical	22863883
XPO1_HUMAN	XPO1	physical	22863883
UBA3_HUMAN	UBA3	physical	15361859
UBC12_HUMAN	UBE2M	physical	19250909
UBE2F_HUMAN	UBE2F	physical	19250909
PLOD1_HUMAN	PLOD1	physical	26344197
UBA3_HUMAN	UBA3	physical	26344197
UBA5_HUMAN	UBA5	physical	26344197
XRCC6_HUMAN	XRCC6	physical	26344197

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• D10413: Pevonedistat (USAN)
• DrugBank
• DB00171: Adenosine triphosphate

Related Literatures of Post-Translational Modification

Acetylation

"Lysine acetylation targets protein complexes and co-regulates majorcellular functions.";
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.;
Science 325:834-840(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-6, AND MASS SPECTROMETRY.
PubMed: 19608861