MKRN1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MKRN1_HUMAN
• UniProt AC: Q9UHC7
• Protein Name: E3 ubiquitin-protein ligase makorin-1
• Gene Name: MKRN1
• Organism: Homo sapiens (Human).
• Sequence Length: 482
• Protein Description: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription..
• Protein Sequence: MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MAEAATPGTTATT --CCCCCCCCCCCCC	29.33	22210691
9	Phosphorylation	AEAATPGTTATTSGA CCCCCCCCCCCCCCH	17.92	28348404
10	Phosphorylation	EAATPGTTATTSGAG CCCCCCCCCCCCCHH	28.11	28348404
12	Phosphorylation	ATPGTTATTSGAGAA CCCCCCCCCCCHHHH	21.28	28348404
13	Phosphorylation	TPGTTATTSGAGAAA CCCCCCCCCCHHHHH	23.77	28348404
14	Phosphorylation	PGTTATTSGAGAAAA CCCCCCCCCHHHHHH	23.45	28348404
22	Phosphorylation	GAGAAAATAAAASPT CHHHHHHHHHHCCCC	16.47	28348404
27	Phosphorylation	AATAAAASPTPIPTV HHHHHHCCCCCCCEE	25.28	28348404
29	Phosphorylation	TAAAASPTPIPTVTA HHHHCCCCCCCEEEC	31.21	28348404
46	Ubiquitination	LGAGGGGGGSDGSGG CCCCCCCCCCCCCCC	35.39	33845483
48	Phosphorylation	AGGGGGGSDGSGGGW CCCCCCCCCCCCCCC	41.91	22210691
49	Ubiquitination	GGGGGGSDGSGGGWT CCCCCCCCCCCCCCH	59.56	27667366
51	Phosphorylation	GGGGSDGSGGGWTKQ CCCCCCCCCCCCHHC	38.98	22210691
67	Ubiquitination	TCRYFMHGVCKEGDN EEEEEECEECCCCCC	18.09	22817900
70	Ubiquitination	YFMHGVCKEGDNCRY EEECEECCCCCCCCC	63.43	21890473
95	Methylation	VVCKYFQRGYCIYGD HHHHHHCCCEEEECC	28.31	115483323
109	Phosphorylation	DRCRYEHSKPLKQEE CCCCCCCCCCCCHHH	24.83	24719451
110	Ubiquitination	RCRYEHSKPLKQEEA CCCCCCCCCCCHHHC	57.17	21890473
110	Ubiquitination	RCRYEHSKPLKQEEA CCCCCCCCCCCHHHC	57.17	33845483
113	Ubiquitination	YEHSKPLKQEEATAT CCCCCCCCHHHCHHH	65.28	27667366
113	Ubiquitination	YEHSKPLKQEEATAT CCCCCCCCHHHCHHH	65.28	21890473
113	Ubiquitination	YEHSKPLKQEEATAT CCCCCCCCHHHCHHH	65.28	21890473
113	Ubiquitination	YEHSKPLKQEEATAT CCCCCCCCHHHCHHH	65.28	21890473
113	Ubiquitination	YEHSKPLKQEEATAT CCCCCCCCHHHCHHH	65.28	21890473
123	Phosphorylation	EATATELTTKSSLAA HCHHHHHCHHHHHHH	26.41	-
131	Phosphorylation	TKSSLAASSSLSSIV HHHHHHHHCCHHHCH	18.17	28634120
132	Phosphorylation	KSSLAASSSLSSIVG HHHHHHHCCHHHCHH	30.48	29802988
133	Phosphorylation	SSLAASSSLSSIVGP HHHHHHCCHHHCHHH	29.76	24719451
133	Ubiquitination	SSLAASSSLSSIVGP HHHHHHCCHHHCHHH	29.76	22817900
135	Phosphorylation	LAASSSLSSIVGPLV HHHHCCHHHCHHHHE	21.70	28634120
136	Phosphorylation	AASSSLSSIVGPLVE HHHCCHHHCHHHHEE	26.96	28348404
138	Ubiquitination	SSSLSSIVGPLVEMN HCCHHHCHHHHEECC	7.54	22817900
146	Ubiquitination	GPLVEMNTGEAESRN HHHEECCCCCHHHCC	34.78	33845483
147	Ubiquitination	PLVEMNTGEAESRNS HHEECCCCCHHHCCC	27.41	22505724
154	Ubiquitination	GEAESRNSNFATVGA CCHHHCCCCCCEECC	32.01	22817900
159	Ubiquitination	RNSNFATVGAGSEDW CCCCCCEECCCCHHH	4.42	22817900
167	Ubiquitination	GAGSEDWVNAIEFVP CCCCHHHCCEEEECC	5.54	27667366
168	Ubiquitination	AGSEDWVNAIEFVPG CCCHHHCCEEEECCC	30.83	22505724
193	Ubiquitination	CTEAPLQGSVTKEES CCCCCCCCCCCHHHH	31.59	21963094
194	Phosphorylation	TEAPLQGSVTKEESE CCCCCCCCCCHHHHH	17.41	28555341
197	Ubiquitination	PLQGSVTKEESEKEQ CCCCCCCHHHHHHHH	58.28	22817900
201	Ubiquitination	SVTKEESEKEQTAVE CCCHHHHHHHHHHHH	64.98	21963094
202	Ubiquitination	VTKEESEKEQTAVET CCHHHHHHHHHHHHH	65.48	21906983
210	Ubiquitination	EQTAVETKKQLCPYA HHHHHHHHHHHCCCC	25.64	33845483
211	Ubiquitination	QTAVETKKQLCPYAA HHHHHHHHHHCCCCE	56.79	22505724
214	Ubiquitination	VETKKQLCPYAAVGE HHHHHHHCCCCEEEE	1.93	21963094
222	Ubiquitination	PYAAVGECRYGENCV CCCEEEECCCCCCCE	3.14	21963094
252	Ubiquitination	HPMDAAQRSQHIKSC CCCCHHHHHHHHHHH	33.29	22817900
257	Ubiquitination	AQRSQHIKSCIEAHE HHHHHHHHHHHHHHH	36.83	27667366
262	Ubiquitination	HIKSCIEAHEKDMEL HHHHHHHHHHHHHHH	9.02	22817900
265	Ubiquitination	SCIEAHEKDMELSFA HHHHHHHHHHHHHHH	53.13	21963094
270	Phosphorylation	HEKDMELSFAVQRSK HHHHHHHHHHHHHCC	9.61	28555341
273	Ubiquitination	DMELSFAVQRSKDMV HHHHHHHHHHCCCCC	4.59	22817900
277	Ubiquitination	SFAVQRSKDMVCGIC HHHHHHCCCCCCCEE	52.62	-
278	Ubiquitination	FAVQRSKDMVCGICM HHHHHCCCCCCCEEE	35.67	21890473
283	Ubiquitination	SKDMVCGICMEVVYE CCCCCCCEEEEHHHH	1.38	22817900
287	Ubiquitination	VCGICMEVVYEKANP CCCEEEEHHHHHCCH	1.90	21963094
290	Ubiquitination	ICMEVVYEKANPSER EEEEHHHHHCCHHHH	33.80	22817900
292	Ubiquitination	MEVVYEKANPSERRF EEHHHHHCCHHHHCE	22.64	22817900
296	Ubiquitination	YEKANPSERRFGILS HHHCCHHHHCEEEEC	49.48	27667366
298	Ubiquitination	KANPSERRFGILSNC HCCHHHHCEEEECCC	30.05	27667366
308	Ubiquitination	ILSNCNHTYCLKCIR EECCCCCHHHHHHHH	11.26	21963094
309	Phosphorylation	LSNCNHTYCLKCIRK ECCCCCHHHHHHHHH	6.55	-
311	Ubiquitination	NCNHTYCLKCIRKWR CCCCHHHHHHHHHHH	3.41	22817900
313	Ubiquitination	NHTYCLKCIRKWRSA CCHHHHHHHHHHHCC	2.04	22817900
316	Ubiquitination	YCLKCIRKWRSAKQF HHHHHHHHHHCCHHH	27.35	22817900
317	Ubiquitination	CLKCIRKWRSAKQFE HHHHHHHHHCCHHHH	6.93	27667366
318	Ubiquitination	LKCIRKWRSAKQFES HHHHHHHHCCHHHHH	29.76	21890473
319	Ubiquitination	KCIRKWRSAKQFESK HHHHHHHCCHHHHHH	38.41	27667366
321	Ubiquitination	IRKWRSAKQFESKII HHHHHCCHHHHHHHH	57.68	21890473
321	Ubiquitination	IRKWRSAKQFESKII HHHHHCCHHHHHHHH	57.68	21890473
321	Ubiquitination	IRKWRSAKQFESKII HHHHHCCHHHHHHHH	57.68	21890473
321	Ubiquitination	IRKWRSAKQFESKII HHHHHCCHHHHHHHH	57.68	21890473
321	Ubiquitination	IRKWRSAKQFESKII HHHHHCCHHHHHHHH	57.68	27667366
326	Ubiquitination	SAKQFESKIIKSCPE CCHHHHHHHHHHCCC	41.05	27667366
329	Ubiquitination	QFESKIIKSCPECRI HHHHHHHHHCCCCEE	49.86	29967540
340	Ubiquitination	ECRITSNFVIPSEYW CCEEECCEECCHHHH	5.44	27667366
351	Ubiquitination	SEYWVEEKEEKQKLI HHHHHCCHHHHHHHH	58.63	21963094
354	Ubiquitination	WVEEKEEKQKLILKY HHCCHHHHHHHHHHH	54.55	22817900
356	Ubiquitination	EEKEEKQKLILKYKE CCHHHHHHHHHHHHH	48.87	22817900
360	Ubiquitination	EKQKLILKYKEAMSN HHHHHHHHHHHHHCC	47.48	27667366
360	Acetylation	EKQKLILKYKEAMSN HHHHHHHHHHHHHCC	47.48	7306337
361	Ubiquitination	KQKLILKYKEAMSNK HHHHHHHHHHHHCCC	15.60	27667366
362	Ubiquitination	QKLILKYKEAMSNKA HHHHHHHHHHHCCCC	37.09	27667366
368	Ubiquitination	YKEAMSNKACRYFDE HHHHHCCCCHHHCCC	41.37	29967540
379	Phosphorylation	YFDEGRGSCPFGGNC HCCCCCCCCCCCCCC	19.77	25159151
389	Ubiquitination	FGGNCFYKHAYPDGR CCCCCCCCCCCCCCC	11.08	33845483
392	Phosphorylation	NCFYKHAYPDGRREE CCCCCCCCCCCCCCC	11.11	22817900
404	Ubiquitination	REEPQRQKVGTSSRY CCCCHHHCCCCHHHH	44.83	27667366
409	Phosphorylation	RQKVGTSSRYRAQRR HHCCCCHHHHHHHHH	32.92	-
411	Phosphorylation	KVGTSSRYRAQRRNH CCCCHHHHHHHHHHH	16.55	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MKRN1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MKRN1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MKRN1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TERT_HUMAN	TERT	physical	15805468
FADD_HUMAN	FADD	physical	22864571
CDN1A_HUMAN	CDKN1A	physical	19536131
IF2B1_MOUSE	Igf2bp1	physical	26265008
HNRPF_MOUSE	Hnrnpf	physical	26265008
ROA3_MOUSE	Hnrnpa3	physical	26265008
CAPR1_MOUSE	Caprin1	physical	26265008
ROA1_MOUSE	Hnrnpa1	physical	26265008
HNRPU_MOUSE	Hnrnpu	physical	26265008
YBOX1_MOUSE	Ybx1	physical	26265008
HNRPQ_MOUSE	Syncrip	physical	26265008
LITD1_MOUSE	L1td1	physical	26265008
PABP1_MOUSE	Pabpc1	physical	26265008
RBMX_MOUSE	Rbmx	physical	26265008
ELAV1_MOUSE	Elavl1	physical	26265008
ROA2_MOUSE	Hnrnpa2b1	physical	26265008
LARP1_MOUSE	Larp1	physical	26265008
G3BP2_MOUSE	G3bp2	physical	26265008
ROAA_MOUSE	Hnrnpab	physical	26265008
ATAD3_MOUSE	Atad3a	physical	26265008
NUFP2_MOUSE	Nufip2	physical	26265008
IF2B3_MOUSE	Igf2bp3	physical	26265008
DHX9_MOUSE	Dhx9	physical	26265008
HNRPD_MOUSE	Hnrnpd	physical	26265008
G3BP1_MOUSE	G3bp1	physical	26265008
LN28A_MOUSE	Lin28a	physical	26265008
PABP2_MOUSE	Pabpn1	physical	26265008
ATX2L_MOUSE	Atxn2l	physical	26265008
MATR3_MOUSE	Matr3	physical	26265008
RENT1_MOUSE	Upf1	physical	26265008
HNRPL_MOUSE	Hnrnpl	physical	26265008
IF4G1_MOUSE	Eif4g1	physical	26265008
UBP10_MOUSE	Usp10	physical	26265008
DDX1_MOUSE	Ddx1	physical	26265008
LARP4_MOUSE	Larp4	physical	26265008
LAR4B_MOUSE	Larp4b	physical	26265008
CN166_MOUSE	2700060E02Rik	physical	26265008
FIP1_MOUSE	Fip1l1	physical	26265008
ILF2_MOUSE	Ilf2	physical	26265008
PRC2C_MOUSE	Prrc2c	physical	26265008
ECHA_MOUSE	Hadha	physical	26265008
CPSF6_MOUSE	Cpsf6	physical	26265008
FA98A_MOUSE	Fam98a	physical	26265008
AGO2_MOUSE	Ago2	physical	26265008
DDX3X_MOUSE	Ddx3x	physical	26265008
MOV10_MOUSE	Mov10	physical	26265008
CASC3_MOUSE	Casc3	physical	26265008
FXR1_MOUSE	Fxr1	physical	26265008
SRRT_MOUSE	Srrt	physical	26265008
PABP1_HUMAN	PABPC1	physical	26265008
IF2B1_HUMAN	IGF2BP1	physical	26265008
ELAV1_HUMAN	ELAVL1	physical	26265008

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.