dbPTM

IF4G1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	IF4G1_MOUSE
UniProt AC	Q6NZJ6
Protein Name	Eukaryotic translation initiation factor 4 gamma 1
Gene Name	Eif4g1
Organism	Mus musculus (Mouse).
Sequence Length	1600
Subcellular Localization
Protein Description	Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome..
Protein Sequence	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
15	Phosphorylation	TGPPPARSPGLPQPA CCCCCCCCCCCCCCC	25.98	27180971
28	Phosphorylation	PAFPPGQTAPVVFST CCCCCCCCCCEEEEC	38.55	25777480
34	Phosphorylation	QTAPVVFSTPQATQM CCCCEEEECCCCCCC	27.80	25777480
35	Phosphorylation	TAPVVFSTPQATQMN CCCEEEECCCCCCCC	14.14	25777480
39	Phosphorylation	VFSTPQATQMNTPSQ EEECCCCCCCCCCCC	24.09	25777480
43	Phosphorylation	PQATQMNTPSQPRQG CCCCCCCCCCCCCCC	20.97	25777480
45	Phosphorylation	ATQMNTPSQPRQGGF CCCCCCCCCCCCCCC	51.26	25777480
62	Methylation	LQHFYPSRAQPPSSA CCHHCCCCCCCCCHH	32.51	-
68	O-linked_Glycosylation	SRAQPPSSAASRVQS CCCCCCCHHHHHHHH	33.36	30059200
72	Methylation	PPSSAASRVQSAAPA CCCHHHHHHHHCCCC	26.26	-
80	Methylation	VQSAAPARPGPAPHV HHHCCCCCCCCCCCC	35.33	-
110	Phosphorylation	YSASQGAYYIPGQGR EEECCCCEECCCCCC	14.57	22817900
117	Methylation	YYIPGQGRSTYVVPT EECCCCCCCEEEEEC	20.24	24129315
186	Phosphorylation	APKRERKTIRIRDPN CCCCCCCCEEECCCC	23.00	23375375
197	Ubiquitination	RDPNQGGKDITEEIM CCCCCCCCCCHHHHH	53.43	22790023
209	Phosphorylation	EIMSGARTASTPTPP HHHCCCCCCCCCCCC	25.15	21082442
211	Phosphorylation	MSGARTASTPTPPQT HCCCCCCCCCCCCCC	34.02	25521595
212	Phosphorylation	SGARTASTPTPPQTG CCCCCCCCCCCCCCC	28.81	25521595
214	Phosphorylation	ARTASTPTPPQTGGS CCCCCCCCCCCCCCC	47.92	25521595
218	Phosphorylation	STPTPPQTGGSLEPQ CCCCCCCCCCCCCCC	49.65	25521595
221	Phosphorylation	TPPQTGGSLEPQPNG CCCCCCCCCCCCCCC	30.79	21082442
230	Phosphorylation	EPQPNGESPQVAVII CCCCCCCCCCEEEEE	23.25	25521595
243	Phosphorylation	IIRPDDRSQGAAIGG EECCCCCCCCCCCCC	39.70	28066266
324	Phosphorylation	TGEPYCLSPEPTLAE CCCCEECCCCCCCCC	24.53	-
347	Phosphorylation	LSKPIPESEFSSSPL ECCCCCHHHCCCCCC	38.29	26643407
350	Phosphorylation	PIPESEFSSSPLQVS CCCHHHCCCCCCEEE	26.34	21149613
351	Phosphorylation	IPESEFSSSPLQVST CCHHHCCCCCCEEEE	41.10	21149613
352	Phosphorylation	PESEFSSSPLQVSTA CHHHCCCCCCEEEEE	28.56	21149613
357	Phosphorylation	SSSPLQVSTALVPHK CCCCCEEEEEECCCE	8.59	26643407
358	Phosphorylation	SSPLQVSTALVPHKV CCCCEEEEEECCCEE	25.75	26643407
376	Phosphorylation	EPNGVIPSEDLEPEV CCCCCCCCCCCCCCC	32.39	23140645
385	Phosphorylation	DLEPEVESSTEPAPP CCCCCCCCCCCCCCC	47.97	23140645
386	Phosphorylation	LEPEVESSTEPAPPP CCCCCCCCCCCCCCC	24.23	23140645
387	Phosphorylation	EPEVESSTEPAPPPL CCCCCCCCCCCCCCC	56.09	23140645
395	Phosphorylation	EPAPPPLSPCASESL CCCCCCCCCCCCCCC	24.57	23140645
399	Phosphorylation	PPLSPCASESLVPIA CCCCCCCCCCCCCCC	34.14	23140645
401	Phosphorylation	LSPCASESLVPIAPT CCCCCCCCCCCCCCC	31.97	23140645
408	Phosphorylation	SLVPIAPTAQPEELL CCCCCCCCCCHHHHH	28.98	23140645
420	Phosphorylation	ELLNGAPSPPAVDLS HHHCCCCCCCCCCCC	42.87	23649490
427	Phosphorylation	SPPAVDLSPVSEPEE CCCCCCCCCCCCHHH	20.84	23140645
430	Phosphorylation	AVDLSPVSEPEEQAK CCCCCCCCCHHHHHH	51.31	23140645
441	Phosphorylation	EQAKKVSSAALASIL HHHHHHCHHHHHHHH	22.13	25521595
446	Phosphorylation	VSSAALASILSPAPP HCHHHHHHHHCCCCC	25.55	25338131
449	Phosphorylation	AALASILSPAPPVAP HHHHHHHCCCCCCCC	19.83	26370283
457	Phosphorylation	PAPPVAPSDTSPAQE CCCCCCCCCCCHHHH	43.73	23737553
459	Phosphorylation	PPVAPSDTSPAQEEE CCCCCCCCCHHHHHH	40.73	23737553
460	Phosphorylation	PVAPSDTSPAQEEEM CCCCCCCCHHHHHHH	24.19	23737553
481	Phosphorylation	EEGGEAESEKGGEDV CCCCCCCHHCCCCCC	52.21	23737553
528	Ubiquitination	KIKELNKKEAVGDLL HHHHHCHHHHHHHHH	50.24	22790023
554	Phosphorylation	EVENQPPTGSNPSPE CCCCCCCCCCCCCCC	60.98	23140645
556	Phosphorylation	ENQPPTGSNPSPESE CCCCCCCCCCCCCCC	48.31	23140645
559	Phosphorylation	PPTGSNPSPESEGSM CCCCCCCCCCCCCCC	45.62	23140645
562	Phosphorylation	GSNPSPESEGSMVPT CCCCCCCCCCCCCCC	51.12	23140645
565	Phosphorylation	PSPESEGSMVPTQPE CCCCCCCCCCCCCCC	16.74	22942356
569	Phosphorylation	SEGSMVPTQPEETEE CCCCCCCCCCCCCCH	45.40	23140645
574	Phosphorylation	VPTQPEETEETWDSK CCCCCCCCCHHCCCC	37.14	23140645
597	Acetylation	NIQPGEQKYEYKSDQ CCCCCCCEEEECCCC	35.43	22826441
597	Ubiquitination	NIQPGEQKYEYKSDQ CCCCCCCEEEECCCC	35.43	22790023
598	Phosphorylation	IQPGEQKYEYKSDQW CCCCCCEEEECCCCC	24.96	25159016
601	Ubiquitination	GEQKYEYKSDQWKPL CCCEEEECCCCCCCC	35.30	22790023
606	Acetylation	EYKSDQWKPLNLEEK EECCCCCCCCCHHHH	33.71	23806337
606	Ubiquitination	EYKSDQWKPLNLEEK EECCCCCCCCCHHHH	33.71	22790023
650	Malonylation	VVLDKANKTPLRQLD HHHHCCCCCCHHHCC	57.27	26320211
651	Phosphorylation	VLDKANKTPLRQLDP HHHCCCCCCHHHCCH	27.62	-
666	Glutathionylation	SRLPGINCGPDFTPS HHCCCCCCCCCCCHH	8.71	24333276
666	S-palmitoylation	SRLPGINCGPDFTPS HHCCCCCCCCCCCHH	8.71	26165157
689	Methylation	LSNRGPPRGGPGGEL HHCCCCCCCCCCCCC	66.87	24129315
698	Methylation	GPGGELPRGPAGLGP CCCCCCCCCCCCCCC	76.09	-
708	Phosphorylation	AGLGPRRSQQGPRKE CCCCCCHHCCCCCHH	28.30	26824392
721	Phosphorylation	KETRKIISSVIMTED HHHHHHHHHHHHHCC	23.25	26239621
722	Phosphorylation	ETRKIISSVIMTEDI HHHHHHHHHHHHCCC	12.78	26824392
726	Phosphorylation	IISSVIMTEDIKLNK HHHHHHHHCCCCCCH	21.27	18779572
770	Phosphorylation	DLFRRVRSILNKLTP HHHHHHHHHHHHHCH	28.68	25159016
774	Ubiquitination	RVRSILNKLTPQMFQ HHHHHHHHHCHHHHH	50.74	22790023
794	Phosphorylation	VTQLAIDTEERLKGV HHHHHCCHHHHHHHH	33.36	18779572
799	Ubiquitination	IDTEERLKGVIDLIF CCHHHHHHHHHHHHH	58.30	22790023
825	S-nitrosocysteine	AYANMCRCLMALKVP HHHHHHHHHHCCCCC	2.31	-
825	S-nitrosylation	AYANMCRCLMALKVP HHHHHHHHHHCCCCC	2.31	21278135
836	Malonylation	LKVPTTEKPTVTVNF CCCCCCCCCEEEEEH	43.85	26320211
885	Ubiquitination	AEERGRLKEELEEAR HHHHHHHHHHHHHHH	48.02	22790023
899	Phosphorylation	RDIARRRSLGNIKFI HHHHHHHCCCCCCHH	38.53	-
904	Acetylation	RRSLGNIKFIGELFK HHCCCCCCHHHHHHH	34.62	22826441
904	Ubiquitination	RRSLGNIKFIGELFK HHCCCCCCHHHHHHH	34.62	22790023
911	Ubiquitination	KFIGELFKLKMLTEA CHHHHHHHHHHHHHH	60.88	22790023
940	S-nitrosylation	EESLECLCRLLTTIG HHHHHHHHHHHHHHC	4.33	24926564
954	Acetylation	GKDLDFAKAKPRMDQ CCCCHHHHCCHHHHH	57.86	23806337
954	Malonylation	GKDLDFAKAKPRMDQ CCCCHHHHCCHHHHH	57.86	26320211
954	Ubiquitination	GKDLDFAKAKPRMDQ CCCCHHHHCCHHHHH	57.86	-
956	Acetylation	DLDFAKAKPRMDQYF CCHHHHCCHHHHHHH	32.23	23201123
968	Acetylation	QYFNQMEKIIKEKKT HHHHHHHHHHHHCCC	44.37	22826441
992	Phosphorylation	DVLDLRQSNWVPRRG HHHHHHHHCCCCCCC	26.65	22871156
1004	Malonylation	RRGDQGPKTIDQIHK CCCCCCCCHHHHHHH	66.01	26320211
1030	Malonylation	KVQQLMAKGSDKRRG HHHHHHHCCCCCCCC	45.01	26320211
1032	Phosphorylation	QQLMAKGSDKRRGGP HHHHHCCCCCCCCCC	38.97	25266776
1036	Methylation	AKGSDKRRGGPPGPP HCCCCCCCCCCCCCC	61.23	24129315
1046	Methylation	PPGPPINRGLPLVDD CCCCCCCCCCCCCCC	49.06	24129315
1058	Phosphorylation	VDDGGWNTVPISKGS CCCCCCCEEECCCCC	22.58	22817900
1062	Phosphorylation	GWNTVPISKGSRPID CCCEEECCCCCCCCC	25.54	28066266
1075	Malonylation	IDTSRLTKITKPGSI CCHHHCCCCCCCCCC	53.63	26320211
1077	Phosphorylation	TSRLTKITKPGSIDS HHHCCCCCCCCCCCC	32.51	25619855
1078	Acetylation	SRLTKITKPGSIDSN HHCCCCCCCCCCCCC	51.35	23806337
1078	Ubiquitination	SRLTKITKPGSIDSN HHCCCCCCCCCCCCC	51.35	22790023
1081	Phosphorylation	TKITKPGSIDSNNQL CCCCCCCCCCCCCCE	31.58	25521595
1084	Phosphorylation	TKPGSIDSNNQLFAP CCCCCCCCCCCEECC	35.69	22942356
1096	Phosphorylation	FAPGGRLSWGKGSSG ECCCCCEECCCCCCC	32.21	26824392
1099	Acetylation	GGRLSWGKGSSGGSG CCCEECCCCCCCCCC	49.73	-
1101	Phosphorylation	RLSWGKGSSGGSGAK CEECCCCCCCCCCCC	29.33	29472430
1102	Phosphorylation	LSWGKGSSGGSGAKP EECCCCCCCCCCCCC	56.70	29472430
1105	Phosphorylation	GKGSSGGSGAKPSDT CCCCCCCCCCCCCCC	38.82	27149854
1110	Phosphorylation	GGSGAKPSDTASEAT CCCCCCCCCCCCHHC	47.06	18846507
1112	Phosphorylation	SGAKPSDTASEATRP CCCCCCCCCCHHCCC	36.10	18846507
1114	Phosphorylation	AKPSDTASEATRPAT CCCCCCCCHHCCCHH	29.83	22817900
1117	Phosphorylation	SDTASEATRPATLNR CCCCCHHCCCHHHHH	32.89	18846507
1121	Phosphorylation	SEATRPATLNRFSAL CHHCCCHHHHHHHHH	27.52	22817900
1126	Phosphorylation	PATLNRFSALQQTLP CHHHHHHHHHHHHCC	25.49	26370283
1131	Phosphorylation	RFSALQQTLPAENTD HHHHHHHHCCCCCCC	23.03	22324799
1137	Phosphorylation	QTLPAENTDNRRVVQ HHCCCCCCCCCHHHH	26.59	22802335
1146	Phosphorylation	NRRVVQRSSLSRERG CCHHHHHHHHHHHHH	20.66	23984901
1147	Phosphorylation	RRVVQRSSLSRERGE CHHHHHHHHHHHHHH	32.33	26824392
1149	Phosphorylation	VVQRSSLSRERGEKA HHHHHHHHHHHHHHH	33.76	26824392
1181	Phosphorylation	DRLDRARTPATKRSF HHHHHCCCHHHHHHH	19.11	26745281
1184	Phosphorylation	DRARTPATKRSFSKE HHCCCHHHHHHHHHH	28.35	26745281
1187	Phosphorylation	RTPATKRSFSKEVEE CCHHHHHHHHHHHHH	35.11	26824392
1189	Phosphorylation	PATKRSFSKEVEERS HHHHHHHHHHHHHHH	29.20	18388127
1190	Acetylation	ATKRSFSKEVEERSR HHHHHHHHHHHHHHH	64.86	23806337
1196	Phosphorylation	SKEVEERSRERPSQP HHHHHHHHHHCCCCC	41.60	26824392
1201	Phosphorylation	ERSRERPSQPEGLRK HHHHHCCCCCHHHHH	65.67	25619855
1208	Malonylation	SQPEGLRKAASLTED CCCHHHHHHHHCCCC	54.04	26320211
1211	Phosphorylation	EGLRKAASLTEDRGR HHHHHHHHCCCCCCC	40.46	27087446
1213	Phosphorylation	LRKAASLTEDRGRDP HHHHHHCCCCCCCCC	33.09	25521595
1217 (in isoform 2)	Phosphorylation	-	51.14	19144319
1226	Phosphorylation	DPVKREATLPPVSPP CCCCCCCCCCCCCCC	34.74	25521595
1231	Phosphorylation	EATLPPVSPPKAALS CCCCCCCCCCCCCCC	41.06	27087446
1238	Phosphorylation	SPPKAALSVDEVEKK CCCCCCCCHHHHHHH	23.85	26824392
1430	Phosphorylation	EYTLGEESEAPGQRT EEECCCCCCCCCHHH	34.45	26525534
1449	Acetylation	ELRRQLEKLLKDGGS HHHHHHHHHHHCCCC	68.65	23864654
1449	Malonylation	ELRRQLEKLLKDGGS HHHHHHHHHHHCCCC	68.65	26320211
1452	Acetylation	RQLEKLLKDGGSNQR HHHHHHHHCCCCCHH	65.84	23954790
1477	Phosphorylation	EQQIASNTLVRALMT HHHHHHHHHHHHHHH	24.92	-
1513	Acetylation	VRARLLQKYLCDEQK HHHHHHHHHCCCHHH	39.91	22826441
1581	Phosphorylation	GKGVALKSVTAFFNW CCCCHHHHHHHHHHH	26.08	22324799
1583	Phosphorylation	GVALKSVTAFFNWLR CCHHHHHHHHHHHHH	25.49	22324799
1597	Phosphorylation	REAEDEESDHN---- HHHHHHHCCCC----	41.96	25521595

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
212	T	Phosphorylation	Kinase	MTOR	Q9JLN9	PSP
1187	S	Phosphorylation	Kinase	PRKCA	P20444	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
1187	S	Phosphorylation		-
Phosphorylation at Ser-1187 by PRKCA induces binding to MKNK1 (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of IF4G1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of IF4G1_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of IF4G1_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry."; Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.; Mol. Cell. Proteomics 8:904-912(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1231 AND SER-1597, ANDMASS SPECTROMETRY.	19131326 [Abstract]
"The phagosomal proteome in interferon-gamma-activated macrophages."; Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.; Immunity 30:143-154(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1231 AND SER-1597, ANDMASS SPECTROMETRY.	19144319 [Abstract]
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1231, AND MASSSPECTROMETRY.	19367708 [Abstract]
"Specific phosphopeptide enrichment with immobilized titanium ionaffinity chromatography adsorbent for phosphoproteome analysis."; Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.; J. Proteome Res. 7:3957-3967(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1211, AND MASSSPECTROMETRY.	18630941 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-212; THR-214 ANDSER-1211, AND MASS SPECTROMETRY.	17242355 [Abstract]

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