NEK2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NEK2_MOUSE
• UniProt AC: O35942
• Protein Name: Serine/threonine-protein kinase Nek2
• Gene Name: Nek2
• Organism: Mus musculus (Mouse).
• Sequence Length: 443
• Subcellular Localization: Nucleus . Nucleus, nucleolus . Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere . STK3/MST2 and SAV1 are required for it
• Protein Description: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (By similarity). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (By similarity). Phosphorylates and activates NEK11 in G1/S-arrested cells. Involved in the regulation of centrosome disjunction (By similarity)..
• Protein Sequence: MPSRVEDYEVLHSIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEVEKQMLVSEVNLLRELKHPNIVSYYDRIIDRTNTTLYIVMEYCEGGDLASVISKGTKDRQYLEEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDSKHNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMSCLSYNEKSDIWSLGCLLYELCALMPPFTAFNQKELAGKIREGRFRRIPYRYSDGLNDLITRMLNLKDYHRPSVEEILESPLIADLVAEEQRRNLERRGRRSGEPSKLPDSSPVLSELKLKERQLQDREQALRAREDILEQKERELCIRERLAEDKLARAESLMKNYSLLKEHRLLCLAGGPELDLPSSAMKKKVHFHGESKENTARSENSESYLAKSKCRDLKKRLHAAQLRAQALADIEKNYQLKSRQILGMR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
41	Phosphorylation	LVWKELDYGSMTEVE EEEEECCCCCCCHHH	24.59	28059163
45	Phosphorylation	ELDYGSMTEVEKQML ECCCCCCCHHHHHHH	38.28	28059163
170	Phosphorylation	ARILNHDTSFAKTFV HHHHCCCCCHHHHCC	20.42	-
171	Phosphorylation	RILNHDTSFAKTFVG HHHCCCCCHHHHCCC	29.30	-
175	Phosphorylation	HDTSFAKTFVGTPYY CCCCHHHHCCCCCCC	21.81	-
179	Phosphorylation	FAKTFVGTPYYMSPE HHHHCCCCCCCCCHH	11.65	-
184	Phosphorylation	VGTPYYMSPEQMSCL CCCCCCCCHHHHHCC	14.79	-
238	Phosphorylation	GRFRRIPYRYSDGLN CCCCCCCCCCCCCHH	21.58	24719451
240	Phosphorylation	FRRIPYRYSDGLNDL CCCCCCCCCCCHHHH	12.79	24719451
241	Phosphorylation	RRIPYRYSDGLNDLI CCCCCCCCCCHHHHH	19.06	24719451
290	Phosphorylation	LERRGRRSGEPSKLP HHHHCCCCCCCCCCC	46.26	22067460
294	Phosphorylation	GRRSGEPSKLPDSSP CCCCCCCCCCCCCCH	42.39	22067460
299	Phosphorylation	EPSKLPDSSPVLSEL CCCCCCCCCHHHHHH	34.30	28066266
300	Phosphorylation	PSKLPDSSPVLSELK CCCCCCCCHHHHHHH	26.80	28066266
356	Phosphorylation	ESLMKNYSLLKEHRL HHHHHHHHHHHHCCC	36.30	-
389	Phosphorylation	KVHFHGESKENTARS CEEECCCCCCCCCCC	50.27	-
396	Phosphorylation	SKENTARSENSESYL CCCCCCCCCCHHHHH	38.37	-
401	Phosphorylation	ARSENSESYLAKSKC CCCCCHHHHHHHHHH	25.85	-
436	Phosphorylation	EKNYQLKSRQILGMR HHHHHCHHHHHHCCC	37.70	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
356	S	Phosphorylation	Kinase	MST2	Q9JI10	Uniprot
436	S	Phosphorylation	Kinase	MST2	Q9JI10	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NEK2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NEK2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
IPP2_HUMAN	PPP1R2	physical	12221103
NEK2_HUMAN	NEK2	physical	20360068
ACAD8_HUMAN	ACAD8	physical	20360068
CDC27_HUMAN	CDC27	physical	26496610
CATC_HUMAN	CTSC	physical	26496610
FOXM1_HUMAN	FOXM1	physical	26496610
FLNA_HUMAN	FLNA	physical	26496610
PDIA3_HUMAN	PDIA3	physical	26496610
KIFC1_HUMAN	KIFC1	physical	26496610
KAP2_HUMAN	PRKAR2A	physical	26496610
PSMD5_HUMAN	PSMD5	physical	26496610
PRP18_HUMAN	PRPF18	physical	26496610
MKNK1_HUMAN	MKNK1	physical	26496610
CDC23_HUMAN	CDC23	physical	26496610
CDC16_HUMAN	CDC16	physical	26496610
WDR46_HUMAN	WDR46	physical	26496610
ITM2B_HUMAN	ITM2B	physical	26496610
DNM1L_HUMAN	DNM1L	physical	26496610
MTDC_HUMAN	MTHFD2	physical	26496610
DLGP4_HUMAN	DLGAP4	physical	26496610
LTN1_HUMAN	LTN1	physical	26496610
FZR1_HUMAN	FZR1	physical	26496610
APC7_HUMAN	ANAPC7	physical	26496610
DEP1A_HUMAN	DEPDC1	physical	26496610
SYIM_HUMAN	IARS2	physical	26496610
HMCES_HUMAN	HMCES	physical	26496610
BBX_HUMAN	BBX	physical	26496610
KNL1_HUMAN	CASC5	physical	26496610
DEFM_HUMAN	PDF	physical	26496610
TFB2M_HUMAN	TFB2M	physical	26496610
APC1_HUMAN	ANAPC1	physical	26496610
CDCA3_HUMAN	CDCA3	physical	26496610
K2013_HUMAN	KIAA2013	physical	26496610
RRFM_HUMAN	MRRF	physical	26496610
EME1_HUMAN	EME1	physical	26496610
CDC26_HUMAN	CDC26	physical	26496610
KIF24_HUMAN	KIF24	physical	26496610

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.