dbPTM

DP13A_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	DP13A_HUMAN
UniProt AC	Q9UKG1
Protein Name	DCC-interacting protein 13-alpha
Gene Name	APPL1
Organism	Homo sapiens (Human).
Sequence Length	709
Subcellular Localization	Early endosome membrane Peripheral membrane protein . Nucleus . Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
Protein Description	Adapter protein that interacts with proteins involved in different cellular signaling pathways. Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. Involved in the regulation of the insulin receptor signaling pathway..
Protein Sequence	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLANIGTSVQNVRREMDSDIETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSSGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELNKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEGGKKRESEA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Ubiquitination	--MPGIDKLPIEETL --CCCCCCCCCHHHH	55.00	24816145
16	Phosphorylation	IEETLEDSPQTRSLL CHHHHCCCCCHHHHH	14.99	21815630
31	Phosphorylation	GVFEEDATAISNYMN HCCHHHHHHHHHHHH	36.52	27732954
34	Phosphorylation	EEDATAISNYMNQLY HHHHHHHHHHHHHHH	21.31	27732954
36	Phosphorylation	DATAISNYMNQLYQA HHHHHHHHHHHHHHH	7.22	27732954
41	Phosphorylation	SNYMNQLYQAMHRIY HHHHHHHHHHHHHHH	5.62	27732954
46	Ubiquitination	QLYQAMHRIYDAQNE HHHHHHHHHHHHHHH	18.97	21963094
49	Ubiquitination	QAMHRIYDAQNELSA HHHHHHHHHHHHHHH	39.03	21890473
53	Ubiquitination	RIYDAQNELSAATHL HHHHHHHHHHHHHHH	31.83	22817900
63	Acetylation	AATHLTSKLLKEYEK HHHHHHHHHHHHHHH	53.24	25953088
63	Ubiquitination	AATHLTSKLLKEYEK HHHHHHHHHHHHHHH	53.24	21963094
66	Ubiquitination	HLTSKLLKEYEKQRF HHHHHHHHHHHHHCC	69.55	27667366
70	Ubiquitination	KLLKEYEKQRFPLGG HHHHHHHHHCCCCCC	45.96	22817900
85	Phosphorylation	DDEVMSSTLQQFSKV CHHHHHHHHHHHHHH	22.68	22468782
97	Phosphorylation	SKVIDELSSCHAVLS HHHHHHHHHCCHHHH	28.95	22817900
98	Phosphorylation	KVIDELSSCHAVLST HHHHHHHHCCHHHHH	23.65	22817900
124	Ubiquitination	QFKERDLKEILTLKE HCCCCCHHHHHCHHH	47.17	29967540
130	Ubiquitination	LKEILTLKEVFQIAS HHHHHCHHHHHHHHC	46.74	29967540
160	Ubiquitination	KRENDKVKYEVTEDV CCCCCCCCEEEEHHH	41.24	29967540
161	Phosphorylation	RENDKVKYEVTEDVY CCCCCCCEEEEHHHH	20.72	27642862
258	Phosphorylation	IEDLEVASDPLYVPD HHHHHHHCCCCCCCC	44.96	-
263	Ubiquitination	VASDPLYVPDPDPTK HHCCCCCCCCCCCCC	5.97	27667366
272	Ubiquitination	DPDPTKFPVNRNLTR CCCCCCCCCCCCHHH	24.83	22053931
280	Ubiquitination	VNRNLTRKAGYLNAR CCCCHHHHHCCCCCC	40.71	27667366
289	Ubiquitination	GYLNARNKTGLVSST CCCCCCCCCCCCCCC	38.12	21890473
290	Phosphorylation	YLNARNKTGLVSSTW CCCCCCCCCCCCCCC	39.02	25394399
294	Phosphorylation	RNKTGLVSSTWDRQF CCCCCCCCCCCCCEE	27.39	25394399
295	Phosphorylation	NKTGLVSSTWDRQFY CCCCCCCCCCCCEEE	26.49	26846344
296	Phosphorylation	KTGLVSSTWDRQFYF CCCCCCCCCCCEEEE	24.71	26846344
311	Phosphorylation	TQGGNLMSQARGDVA ECCCCHHHHHCCCCC	24.69	25394399
333	Ubiquitination	DNCSVMAVDCEDRRY CCCEEEEEECCCCEE	4.70	22505724
343	Ubiquitination	EDRRYCFQITSFDGK CCCEEEEEEEECCCC	34.19	27667366
350	Acetylation	QITSFDGKKSSILQA EEEECCCCCCCCEEC	51.86	22361671
350	Ubiquitination	QITSFDGKKSSILQA EEEECCCCCCCCEEC	51.86	22505724
351	Ubiquitination	ITSFDGKKSSILQAE EEECCCCCCCCEECC	55.77	29967540
352	Phosphorylation	TSFDGKKSSILQAES EECCCCCCCCEECCC	26.54	-
360	Ubiquitination	SILQAESKKDHEEWI CCEECCCCCCCHHHE	54.44	27667366
361	Ubiquitination	ILQAESKKDHEEWIC CEECCCCCCCHHHEE	73.86	29967540
374	Phosphorylation	ICTINNISKQIYLSE EEEECCCCCEEEECC	22.19	22817900
375	Ubiquitination	CTINNISKQIYLSEN EEECCCCCEEEECCC	36.60	29967540
378	Phosphorylation	NNISKQIYLSENPEE CCCCCEEEECCCHHH	11.15	29978859
386	Phosphorylation	LSENPEETAARVNQS ECCCHHHHHHHHHHH	24.77	28555341
393	Phosphorylation	TAARVNQSALEAVTP HHHHHHHHHHHHHCC	29.62	29255136
399	Phosphorylation	QSALEAVTPSPSFQQ HHHHHHHCCCHHHHH	25.70	29255136
401	Phosphorylation	ALEAVTPSPSFQQRH HHHHHCCCHHHHHHH	24.66	19664994
403	Phosphorylation	EAVTPSPSFQQRHES HHHCCCHHHHHHHHH	39.77	29255136
410	Phosphorylation	SFQQRHESLRPAAGQ HHHHHHHHHCCCCCC	24.45	26657352
418	Phosphorylation	LRPAAGQSRPPTART HCCCCCCCCCCCCCC	45.92	25002506
422	Phosphorylation	AGQSRPPTARTSSSG CCCCCCCCCCCCCCC	31.30	26074081
425	Phosphorylation	SRPPTARTSSSGSLG CCCCCCCCCCCCCCC	30.42	27080861
426	Phosphorylation	RPPTARTSSSGSLGS CCCCCCCCCCCCCCC	19.56	27080861
427	Phosphorylation	PPTARTSSSGSLGSE CCCCCCCCCCCCCCC	37.80	24400094
428	Phosphorylation	PTARTSSSGSLGSES CCCCCCCCCCCCCCH	31.97	26074081
430	Phosphorylation	ARTSSSGSLGSESTN CCCCCCCCCCCCHHC	31.35	29496963
433	Phosphorylation	SSSGSLGSESTNLAA CCCCCCCCCHHCEEE	33.40	29496963
435	Phosphorylation	SGSLGSESTNLAALS CCCCCCCHHCEEEEC	25.05	27080861
436	Phosphorylation	GSLGSESTNLAALSL CCCCCCHHCEEEECC	30.14	28464451
442	Phosphorylation	STNLAALSLDSLVAP HHCEEEECCCCCCCC	25.96	27080861
445	Phosphorylation	LAALSLDSLVAPDTP EEEECCCCCCCCCCC	30.00	27080861
451	Phosphorylation	DSLVAPDTPIQFDII CCCCCCCCCEEEEEE	22.64	27080861
459	Phosphorylation	PIQFDIISPVCEDQP CEEEEEEECCCCCCC	16.18	22817900
473	Ubiquitination	PGQAKAFGQGGRRTN CCCCCCCCCCCCCCC	30.04	22817900
479	Phosphorylation	FGQGGRRTNPFGESG CCCCCCCCCCCCCCC	45.97	23312004
485	Phosphorylation	RTNPFGESGGSTKSE CCCCCCCCCCCCCCC	49.16	28348404
488	Phosphorylation	PFGESGGSTKSETED CCCCCCCCCCCCCHH	35.87	28348404
489	Phosphorylation	FGESGGSTKSETEDS CCCCCCCCCCCCHHH	41.69	24719451
490	Ubiquitination	GESGGSTKSETEDSI CCCCCCCCCCCHHHH	48.25	21906983
491	Phosphorylation	ESGGSTKSETEDSIL CCCCCCCCCCHHHHH	50.25	22817900
493	Phosphorylation	GGSTKSETEDSILHQ CCCCCCCCHHHHHHH	52.78	28857561
496	Ubiquitination	TKSETEDSILHQLFI CCCCCHHHHHHHHHH	22.33	22817900
496	Phosphorylation	TKSETEDSILHQLFI CCCCCHHHHHHHHHH	22.33	23312004
509	Phosphorylation	FIVRFLGSMEVKSDD HHHHHHCCCEECCCC	17.57	29759185
513	Ubiquitination	FLGSMEVKSDDHPDV HHCCCEECCCCCCHH	34.56	21906983
514	Phosphorylation	LGSMEVKSDDHPDVV HCCCEECCCCCCHHH	54.14	-
522	Phosphorylation	DDHPDVVYETMRQIL CCCCHHHHHHHHHHH	12.91	25147952
524	Phosphorylation	HPDVVYETMRQILAA CCHHHHHHHHHHHHH	10.44	-
590	Phosphorylation	LFGFVLRTSSGRSES EEEEEEECCCCCCCC	24.08	27251275
591	Phosphorylation	FGFVLRTSSGRSESN EEEEEECCCCCCCCC	25.10	27251275
592	Phosphorylation	GFVLRTSSGRSESNL EEEEECCCCCCCCCC	37.30	27251275
595	Phosphorylation	LRTSSGRSESNLSSV EECCCCCCCCCCCCE	49.23	27251275
597	Phosphorylation	TSSGRSESNLSSVCY CCCCCCCCCCCCEEE	44.30	27251275
604	Phosphorylation	SNLSSVCYIFESNNE CCCCCEEEEEECCCC	13.39	22817900
621	Ubiquitination	KICDSVGLAKQIALH EEHHHHCHHHHHHHH	5.15	24816145
623	Ubiquitination	CDSVGLAKQIALHAE HHHHCHHHHHHHHHH	47.48	29967540
636	Phosphorylation	AELDRRASEKQKEIE HHHHHHHHHHHHHHH	43.44	27251275
638	Ubiquitination	LDRRASEKQKEIERV HHHHHHHHHHHHHHH	65.26	24816145
644	Ubiquitination	EKQKEIERVKEKQQK HHHHHHHHHHHHHHH	50.93	22053931
655	Ubiquitination	KQQKELNKQKQIEKD HHHHHHHHHHHHHHH	72.06	24816145
661	Ubiquitination	NKQKQIEKDLEEQSR HHHHHHHHHHHHHHH	70.06	21890473
673	Phosphorylation	QSRLIAASSRPNQAS HHHHHHHHCCCCCCC	20.18	29978859
674	Phosphorylation	SRLIAASSRPNQASS HHHHHHHCCCCCCCC	47.21	23312004
680	Phosphorylation	SSRPNQASSEGQFVV HCCCCCCCCCCCEEE	21.11	23312004
681	Phosphorylation	SRPNQASSEGQFVVL CCCCCCCCCCCEEEE	49.08	29978859
689	Phosphorylation	EGQFVVLSSSQSEES CCCEEEEECCCCCCC	19.14	26657352
690	Phosphorylation	GQFVVLSSSQSEESD CCEEEEECCCCCCCC	28.38	28464451
691	Phosphorylation	QFVVLSSSQSEESDL CEEEEECCCCCCCCC	34.06	17525332
693	Phosphorylation	VVLSSSQSEESDLGE EEEECCCCCCCCCCC	45.33	25849741
696	Phosphorylation	SSSQSEESDLGEGGK ECCCCCCCCCCCCCC	33.80	30576142
703	Ubiquitination	SDLGEGGKKRESEA- CCCCCCCCCCCCCC-	60.56	32015554

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
410	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
410	S	Phosphorylation	Kinase	PKA	-	Uniprot
430	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
430	S	Phosphorylation	Kinase	PKCA	P20444	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	NEDD4	P46934	PMID:21320486

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
410	S	Phosphorylation		17765681
Phosphorylation at Ser-410 by PKA severely impairs binding to OCRL.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of DP13A_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DP13B_HUMAN	APPL2	physical	16189514
AKT2_HUMAN	AKT2	physical	10490823
DCC_HUMAN	DCC	physical	12011067
MTA2_HUMAN	MTA2	physical	19686092
HDAC1_HUMAN	HDAC1	physical	19686092
HDAC2_HUMAN	HDAC2	physical	19686092
RBBP4_HUMAN	RBBP4	physical	19686092
RBBP7_HUMAN	RBBP7	physical	19686092
RUVB2_HUMAN	RUVBL2	physical	19433865
MTA2_HUMAN	MTA2	physical	15016378
RBBP7_HUMAN	RBBP7	physical	15016378
FSHR_HUMAN	FSHR	physical	15070827
PAQR1_HUMAN	ADIPOR1	physical	16622416
RAB5A_HUMAN	RAB5A	physical	16622416
DP13B_HUMAN	APPL2	physical	17030088
AKT2_HUMAN	AKT2	physical	17030088
DP13A_HUMAN	APPL1	physical	17581628
P85A_HUMAN	PIK3R1	physical	12621049
AKT1_HUMAN	AKT1	physical	12621049
ANDR_HUMAN	AR	physical	12621049
HDAC3_HUMAN	HDAC3	physical	23752268
PAQR1_HUMAN	ADIPOR1	physical	23762377
TRAF6_HUMAN	TRAF6	physical	23909487
DPYL5_HUMAN	DPYSL5	physical	21988832
KXDL1_HUMAN	KXD1	physical	21988832
CKLF4_HUMAN	CMTM4	physical	21988832
K1468_HUMAN	KIAA1468	physical	22863883
TTC4_HUMAN	TTC4	physical	22863883
DP13A_HUMAN	APPL1	physical	25416956
DP13B_HUMAN	APPL2	physical	25416956
UBE2O_HUMAN	UBE2O	physical	25416956
REBL1_HUMAN	RHEBL1	physical	25416956
BATF3_HUMAN	BATF3	physical	25814554
BRWD1_HUMAN	BRWD1	physical	25814554
RTCB_HUMAN	RTCB	physical	25814554
DOK3_HUMAN	DOK3	physical	25814554
DOK7_HUMAN	DOK7	physical	25814554
IN80E_HUMAN	INO80E	physical	25814554
KLF15_HUMAN	KLF15	physical	25814554
MAGC3_HUMAN	MAGEC3	physical	25814554
ZN829_HUMAN	ZNF829	physical	25814554
CBL_HUMAN	CBL	physical	25814554
CBLB_HUMAN	CBLB	physical	25814554
DOK2_HUMAN	DOK2	physical	25814554
ID1_HUMAN	ID1	physical	25814554
P85A_HUMAN	PIK3R1	physical	25814554
SH22A_HUMAN	SH2D2A	physical	25814554
SOCS6_HUMAN	SOCS6	physical	25814554
SCAPE_HUMAN	SCAPER	physical	25814554
AKT1_HUMAN	AKT1	physical	25814554
P85B_HUMAN	PIK3R2	physical	25814554
P53_HUMAN	TP53	physical	25814554
HDAC1_HUMAN	HDAC1	physical	25241761
TRAF2_HUMAN	TRAF2	physical	25241761
PK3CA_HUMAN	PIK3CA	physical	25241761
AKT1_HUMAN	AKT1	physical	25241761
TGFR1_HUMAN	TGFBR1	physical	26583432
KPCZ_HUMAN	PRKCZ	physical	26583432
TBB3_HUMAN	TUBB3	physical	26583432
ZN830_HUMAN	ZNF830	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of DP13A_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-401, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-399 AND SER-401, ANDMASS SPECTROMETRY.	18669648 [Abstract]
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-691, AND MASSSPECTROMETRY.	17525332 [Abstract]
"A role of the Lowe syndrome protein OCRL in early steps of theendocytic pathway."; Erdmann K.S., Mao Y., McCrea H.J., Zoncu R., Lee S., Paradise S.,Modregger J., Biemesderfer D., Toomre D., De Camilli P.; Dev. Cell 13:377-390(2007). Cited for: INTERACTION WITH OCRL, F&H MOTIF, AND PHOSPHORYLATION AT SER-410.	17765681 [Abstract]

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