PKHO1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PKHO1_HUMAN
• UniProt AC: Q53GL0
• Protein Name: Pleckstrin homology domain-containing family O member 1
• Gene Name: PLEKHO1
• Organism: Homo sapiens (Human).
• Sequence Length: 409
• Subcellular Localization: Cell membrane ; Peripheral membrane protein . Nucleus . Cytoplasm . Predominantly localized to the plasma membrane through the binding to phosphatidylinositol 3-phosphate (PubMed:14729969). In C2C12 cells, with the absence of growth factor, it is fou
• Protein Description: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation..
• Protein Sequence: MMKKNNSAKRGPQDGNQQPAPPEKVGWVRKFCGKGIFREIWKNRYVVLKGDQLYISEKEVKDEKNIQEVFDLSDYEKCEELRKSKSRSKKNHSKFTLAHSKQPGNTAPNLIFLAVSPEEKESWINALNSAITRAKNRILDEVTVEEDSYLAHPTRDRAKIQHSRRPPTRGHLMAVASTSTSDGMLTLDLIQEEDPSPEEPTSCAESFRVDLDKSVAQLAGSRRRADSDRIQPSADRASSLSRPWEKTDKGATYTPQAPKKLTPTEKGRCASLEEILSQRDAASARTLQLRAEEPPTPALPNPGQLSRIQDLVARKLEETQELLAEVQGLGDGKRKAKDPPRSPPDSESEQLLLETERLLGEASSNWSQAKRVLQEVRELRDLYRQMDLQTPDSHLRQTTPHSQYRKSLM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Phosphorylation	-MMKKNNSAKRGPQD -CCCCCCCCCCCCCC	44.56	29978859
143 (in isoform 2)	Phosphorylation	-	21.83	22210691
143	Phosphorylation	NRILDEVTVEEDSYL CCCCCEEEECCCCCC	21.83	29978859
144 (in isoform 2)	Phosphorylation	-	10.28	22210691
148	Phosphorylation	EVTVEEDSYLAHPTR EEEECCCCCCCCCCC	25.89	29978859
149	Phosphorylation	VTVEEDSYLAHPTRD EEECCCCCCCCCCCC	21.89	29978859
154	Phosphorylation	DSYLAHPTRDRAKIQ CCCCCCCCCCCHHHC	35.02	29978859
172 (in isoform 2)	Phosphorylation	-	2.34	22210691
214	Phosphorylation	FRVDLDKSVAQLAGS HCCCCCHHHHHHHHH	23.50	24117733
221	Phosphorylation	SVAQLAGSRRRADSD HHHHHHHHHCCCCCC	19.60	20164059
227	Phosphorylation	GSRRRADSDRIQPSA HHHCCCCCCCCCCCH	28.01	23401153
233	Phosphorylation	DSDRIQPSADRASSL CCCCCCCCHHHHHHC	26.82	23403867
238	Phosphorylation	QPSADRASSLSRPWE CCCHHHHHHCCCCCC	32.48	28102081
239	Phosphorylation	PSADRASSLSRPWEK CCHHHHHHCCCCCCC	29.40	23401153
241	Phosphorylation	ADRASSLSRPWEKTD HHHHHHCCCCCCCCC	37.94	29978859
247	Phosphorylation	LSRPWEKTDKGATYT CCCCCCCCCCCCCCC	31.28	26074081
252	Phosphorylation	EKTDKGATYTPQAPK CCCCCCCCCCCCCCC	36.27	23403867
253	Phosphorylation	KTDKGATYTPQAPKK CCCCCCCCCCCCCCC	18.85	25884760
254	Phosphorylation	TDKGATYTPQAPKKL CCCCCCCCCCCCCCC	12.45	23403867
262	Phosphorylation	PQAPKKLTPTEKGRC CCCCCCCCCCCCCCC	36.72	26657352
264	Phosphorylation	APKKLTPTEKGRCAS CCCCCCCCCCCCCCC	45.13	26074081
271	Phosphorylation	TEKGRCASLEEILSQ CCCCCCCCHHHHHCC	39.68	19664994
277	Phosphorylation	ASLEEILSQRDAASA CCHHHHHCCHHHHHH	28.91	23403867
342	Phosphorylation	KAKDPPRSPPDSESE CCCCCCCCCCCCHHH	46.70	30576142
346	Phosphorylation	PPRSPPDSESEQLLL CCCCCCCCHHHHHHH	49.12	30108239
348	Phosphorylation	RSPPDSESEQLLLET CCCCCCHHHHHHHHH	34.30	30108239
363	Phosphorylation	ERLLGEASSNWSQAK HHHHHHHCCCHHHHH	21.65	-
364	Phosphorylation	RLLGEASSNWSQAKR HHHHHHCCCHHHHHH	49.74	-
367	Phosphorylation	GEASSNWSQAKRVLQ HHHCCCHHHHHHHHH	25.22	-
383	Phosphorylation	VRELRDLYRQMDLQT HHHHHHHHHHHCCCC	11.77	29978859
399	Phosphorylation	DSHLRQTTPHSQYRK CHHHCCCCCCHHHHH	15.63	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PKHO1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PKHO1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PKHO1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SMUF1_HUMAN	SMURF1	physical	18641638
SMAD5_HUMAN	SMAD5	physical	18641638
SMUF1_HUMAN	SMURF1	physical	20580715
SMUF1_HUMAN	SMURF1	physical	20484049
PKHO1_HUMAN	PLEKHO1	physical	18624398
DNJB1_HUMAN	DNAJB1	physical	18624398
CJ088_HUMAN	C10orf88	physical	18624398
FLNB_HUMAN	FLNB	physical	18624398
RS20_HUMAN	RPS20	physical	18624398
IN35_HUMAN	IFI35	physical	18624398
PRS8_HUMAN	PSMC5	physical	23032291
SMUF1_HUMAN	SMURF1	physical	23032291
CHIP_MOUSE	Stub1	physical	20368287
ARC1A_MOUSE	Arpc1a	physical	20368287
RS3A_MOUSE	Rps3a1	physical	20368287
AKT1_HUMAN	AKT1	physical	17942896
AKT2_HUMAN	AKT2	physical	17942896
PKHO1_HUMAN	PLEKHO1	physical	17942896
SC24D_HUMAN	SEC24D	physical	28514442
CENPV_HUMAN	CENPV	physical	28514442
CAZA1_HUMAN	CAPZA1	physical	28514442
CAPZB_HUMAN	CAPZB	physical	28514442
RL26L_HUMAN	RPL26L1	physical	28514442
RRP5_HUMAN	PDCD11	physical	28514442
UBA6_HUMAN	UBA6	physical	28514442
FLNC_HUMAN	FLNC	physical	28514442
SPT2_HUMAN	SPTY2D1	physical	28514442
TRM1L_HUMAN	TRMT1L	physical	28514442
NMNA1_HUMAN	NMNAT1	physical	28514442
WIPI3_HUMAN	WDR45B	physical	28514442
NVL_HUMAN	NVL	physical	28514442
FXR1_HUMAN	FXR1	physical	28514442
NOC2L_HUMAN	NOC2L	physical	28514442
GTPBA_HUMAN	GTPBP10	physical	28514442
NPA1P_HUMAN	URB1	physical	28514442
DDX31_HUMAN	DDX31	physical	28514442
RL3_HUMAN	RPL3	physical	28514442
UBP7_HUMAN	USP7	physical	28514442
DDX51_HUMAN	DDX51	physical	28514442
GTPB2_HUMAN	GTPBP2	physical	28514442
NOC3L_HUMAN	NOC3L	physical	28514442
SPT5H_HUMAN	SUPT5H	physical	28514442
DOK2_HUMAN	DOK2	physical	28514442
MBB1A_HUMAN	MYBBP1A	physical	28514442
STAU1_HUMAN	STAU1	physical	28514442
SDA1_HUMAN	SDAD1	physical	28514442
SPB1_HUMAN	FTSJ3	physical	28514442
GLYR1_HUMAN	GLYR1	physical	28514442
NLE1_HUMAN	NLE1	physical	28514442
KBTB7_HUMAN	KBTBD7	physical	28514442
RL5_HUMAN	RPL5	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.