MAD_DROME - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MAD_DROME
• UniProt AC: P42003
• Protein Name: Protein mothers against dpp
• Gene Name: Mad
• Organism: Drosophila melanogaster (Fruit fly).
• Sequence Length: 455
• Subcellular Localization: Cytoplasm . Nucleus .
• Protein Description: Required for the function of decapentaplegic. May play an important role in mediating Dpp signaling. Involved in the BMP signaling pathway..
• Protein Sequence: MDTDDVESNTSSAMSTLGSLFSFTSPAVKKLLGWKQGDEEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKRVESPVLPPVLVPRHSEFAPGHSMLQFNHVAEPSMPHNVSYSNSGFNSHSLSTSNTSVGSPSSVNSNPNSPYDSLAGTPPPAYSPSEDGNSNNPNDGGQLLDAQMGDVAQVSYSEPAFWASIAYYELNCRVGEVFHCNNNSVIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRRHIGKGVHLYYVTGEVYAECLSDSAIFVQSRNCNYHHGFHPSTVCKIPPGCSLKIFNNQEFAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNAISSVS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
25	Phosphorylation	GSLFSFTSPAVKKLL HHHHHCCCHHHHHHH	14.62	17507407
453	Phosphorylation	SPHNAISSVS----- CCCHHHCCCC-----	21.92	18327897
455	Phosphorylation	HNAISSVS------- CHHHCCCC-------	36.13	18327897

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MAD_DROME !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MAD_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MAD_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NUMB_DROME	numb	physical	14605208
CPN_DROME	Cpn	physical	14605208
UBCD2_DROME	UbcD2	physical	14605208
BCD_DROME	bcd	physical	15575970
2ABA_DROME	tws	physical	15575970
NPL4_DROME	Npl4	physical	15575970
DAN_DROME	dan	physical	15575970
COG5_DROME	fws	physical	15575970
BOWEL_DROME	bowl	physical	15575970
MED15_DROME	MED15	physical	15575970
PANG1_DROME	pan	physical	15575970
PANG2_DROME	pan	physical	15575970
TAMO_DROME	tamo	physical	15575970
SUDX_DROME	Su(dx)	physical	15575970
VPS18_DROME	dor	physical	15575970
SMUF1_DROME	lack	physical	15575970
Y3427_DROME	CG43427	physical	15575970
YAP1_DROME	yki	physical	19914168
EYA_DROME	eya	genetic	10683184
WNTG_DROME	wg	genetic	21990430
YAP1_DROME	yki	genetic	21238929
INHB_DROME	Actbeta	genetic	18820452
ERKA_DROME	rl	genetic	17507407
ARM_DROME	arm	physical	21990430
CNEP1_DROME	Dd	physical	27578171
MAD_DROME	Mad	physical	9693372
MAD_DROME	Mad	physical	19557331
FOXF_DROME	bin	physical	23935523
PANG1_DROME	pan	physical	21990430
PANG2_DROME	pan	physical	21990430

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteome analysis of Drosophila melanogaster embryos.";
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
J. Proteome Res. 7:1675-1682(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-453 AND SER-455, ANDMASS SPECTROMETRY.
PubMed: 18327897

"Drosophila Nemo antagonizes BMP signaling by phosphorylation of Madand inhibition of its nuclear accumulation.";
Zeng Y.A., Rahnama M., Wang S., Sosu-Sedzorme W., Verheyen E.M.;
Development 134:2061-2071(2007).
Cited for: FUNCTION, SUBCELLULAR LOCATION, AND PHOSPHORYLATION AT SER-25.
PubMed: 17507407

"DSmurf selectively degrades decapentaplegic-activated MAD, and itsoverexpression disrupts imaginal disc development.";
Liang Y.-Y., Lin X., Liang M., Brunicardi F.C., ten Dijke P., Chen Z.,Choi K.-W., Feng X.-H.;
J. Biol. Chem. 278:26307-26310(2003).
Cited for: INTERACTION WITH LACK, PHOSPHORYLATION AT SER-453 AND SER-455,MUTAGENESIS OF SER-453 AND SER-455, AND UBIQUITINATION.
PubMed: 12754252