dbPTM

GAGA_DROME - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	GAGA_DROME
UniProt AC	Q08605
Protein Name	Transcription factor GAGA
Gene Name	Trl
Organism	Drosophila melanogaster (Fruit fly).
Sequence Length	581
Subcellular Localization	Nucleus .
Protein Description	Transcriptional activator that functions by regulating chromatin structure. Overcomes the repressive effects of chromatin by promoting the open chromatin conformation in promoter gene regions, thereby allowing access to other transcription factors. Binds to DNA Polycomb response elements (PREs) at the bithorax complex and to the proximal region of the engrailed promoter, and positively regulates transcription of many genes including homeotic ones. Binds to the DNA sequence (GA)n, with optimal binding to the pentamer 5'-GAGAG-3'. Binds DNA as an oligomer. May also act as a transcriptional repressor, maintaining the repressed state of genes including lolal, and down-regulating its own transcription. Required for dosage compensation in males and may be involved in oogenesis. Also has a role in nuclear division..
Protein Sequence	MSLPMNSLYSLTWGDYGTSLVSAIQLLRCHGDLVDCTLAAGGRSFPAHKIVLCAASPFLLDLLKNTPCKHPVVMLAGVNANDLEALLEFVYRGEVSVDHAQLPSLLQAAQCLNIQGLAPQTVTKDDYTTHSIQLQHMIPQHHDQDQLIATIATAPQQTVHAQVVEDIHHQGQILQATTQTNAAGQQQTIVTTDAAKHDQAVIQAFLPARKRKPRVKKMSPTAPKISKVEGMDTIMGTPTSSHGSGSVQQVLGENGAEGQLLSSTPIIKSEGQKVETIVTMDPNNMIPVTSANAATGEITPAQGATGSSGGNTSGVLSTPKAKRAKHPPGTEKPRSRSQSEQPATCPICYAVIRQSRNLRRHLELRHFAKPGVKKEKKTTSGKKSSSGSSGSGSGALSSSGSVPQVQTVQSLHTLQGVQVKKDPDAQQQQQQQQQQQQQQQQAMTVSGATGGQVQQQVQQVQQQVQQQQQQQQQQQQQLQHHQIIDSSGNITTATTSAQAAAAAQQQAAGQQQQLVAQSDGSESGAPLSIAQVQTLQGHQIIGNLNQVNMTDFQQQQPQQQQQQQQQQQQQQQQQQQTQQTL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
219	Phosphorylation	KPRVKKMSPTAPKIS CCCCCCCCCCCCCCC	28.16	19429919
221	Phosphorylation	RVKKMSPTAPKISKV CCCCCCCCCCCCCEE	48.57	19429919
237	Phosphorylation	GMDTIMGTPTSSHGS CCCEECCCCCCCCCC	13.15	22817900
325	Acetylation	TPKAKRAKHPPGTEK CCHHHHCCCCCCCCC	61.75	20849112
335	Phosphorylation	PGTEKPRSRSQSEQP CCCCCCCCCCCCCCC	44.82	21082442
373	Acetylation	HFAKPGVKKEKKTTS HHCCCCCCCCEECCC	62.22	20849112
388	Phosphorylation	GKKSSSGSSGSGSGA CCCCCCCCCCCCCCC	33.21	16023138
404 (in isoform 2)	Phosphorylation	-	37.48	22668510

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
378	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
388	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
388	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
388	S	Phosphorylation	Kinase	CKII_GROUP	-	PhosphoELM

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of GAGA_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of GAGA_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GAGA_DROME	Trl	physical	12815073
SSRP1_DROME	Ssrp	physical	12815073
SPT16_DROME	dre4	physical	12815073
HIRA_DROME	Hira	physical	17344416
MSL2_DROME	msl-2	genetic	15020425
MSL1_DROME	msl-1	genetic	15020425
NELFE_DROME	Nelf-E	genetic	27468311
CORTO_DROME	corto	genetic	11810228
NELFA_DROME	Nelf-A	genetic	27468311
NU301_DROME	E(bx)	genetic	27468311
MMD4_DROME	mod(mdg4)	genetic	9491892
GAGA_DROME	Trl	physical	23847101
GAGA_DROME	Trl	physical	9927429
RBF_DROME	Rbf	physical	23070566
ACT1_DROME	Act5C	physical	23136390
HIRA_DROME	Hira	physical	23136390
FSH_DROME	fs(1)h	physical	23945939
BAP60_DROME	Bap60	physical	23136390
ESC_DROME	esc	physical	11581156
ISWI_DROME	Iswi	physical	23136390
SSRP1_DROME	Ssrp	physical	23136390
KEN_DROME	ken	physical	23847101
YAP1_DROME	yki	physical	23395637
SPT16_DROME	dre4	physical	23136390
HDAC1_DROME	Rpd3	physical	23136390
THOC6_DROME	thoc6	physical	23136390
BRM_DROME	brm	physical	23136390
CHDM_DROME	Mi-2	physical	23136390
PC_DROME	Pc	physical	11581156
NU301_DROME	E(bx)	physical	23136390
TTKB_DROME	ttk	physical	25242320
TTKA_DROME	ttk	physical	25242320
TTKB_DROME	ttk	physical	12384587
TTKA_DROME	ttk	physical	12384587

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of GAGA_DROME

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Phosphoproteome analysis of Drosophila melanogaster embryos."; Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.; J. Proteome Res. 7:1675-1682(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-237, AND MASSSPECTROMETRY.	18327897 [Abstract]