NU301_DROME - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NU301_DROME
• UniProt AC: Q9W0T1
• Protein Name: Nucleosome-remodeling factor subunit NURF301
• Gene Name: E(bx)
• Organism: Drosophila melanogaster (Fruit fly).
• Sequence Length: 2669
• Subcellular Localization: Nucleus .
• Protein Description: Histone-binding component of NURF (nucleosome remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. Required for homeotic gene expression, proper larval blood cell development, normal male X chromosome morphology, ecdysteroid signaling and metamorphosis..
• Protein Sequence: MSGRGSRKRGRPPKTPNERASGRFNYQLLKKPKYLSEGKSQPSTPSASRGISPQSDEGSRSSHNNHTNRSRGSAAKRGRGRKSAVQPNTSSYSGRKGYESEYHYGSDFGDSEEDKSDNEDDMLLTPSDDESLEVANESESEFSVCSFNQNGVGRPPRPPSPEPVWLQEGRQYAALDLPDSSEDLFIANTHVLRALSIYEVLRRFRHMVRLSPFRFEDLCAALACEEQSALLTEVHIMLLKAILREEDAQGTHFGPLDQKDTVNISLYLIDSITWPEVLRSYVESDKTFDRNVFHILSHTEYPYTGIDNRLEVLQFLSDQFLTSNSIRDVMLQEGPIHYDDHCRVCHRLGDLLCCETCPAVYHLECVDPPMNDVPTEDWQCGLCRSHKVSGVVDCVLPQEKQGVLIRHDSLGVDRHGRKYWFIARRIFIEDQENFTCWYYSTTSKLKLLLSRLDAEELETRLHSQITERRDEIERQMKLTETLTNEHKHTKRSVIEIEQEAKNELLEKEVLDEDEKDGDAKSESQSIEGTKKQEECKMVTRQKSNQLTNGTLHFKLGMEQGFKNYVNQYSTNPIALNKPQRNEERDKRRHLSHKFSLTTASDFKWIGITMGTTDNMITTLRQTLINFESNIAASFLNINWVVNKKIWNAAVMNARRPSEFAVVLLLFQASLKSVVFANVWHEQLGHTTLQRITSAEREERKKLEKREKRERDDEEERNRLAFNYIKYTLGLKHQVWKQKGEEYRVHGQWGWLWLSSSRRCGVRARRAQPLTHNRVYVHYTMGEENDVNEIILVDPRTQRFMQQCESSNVDGQVCHYLPDQYKNVKVIEDVTEKIKGHIDVSKALNAPGRTYYSKVARKSRLDDLLDRRLKLAEVEEQMASKIPSDMKPLLVSSQNNTANSKQTFLEKRLLRLTEVQAKGGPANVNLELVNSLAKQIQTVRLQFSQLNRFAKVFRCYTKECNTNSNAVSQITQNTCYSPLCLQKARAKKELLLLLRKAHTAGNGSKETVAAILGAVKKPSILEQKLTEGKRESTQVAVDDSEEGKPAESEAPLDLLQDWEHARAHAVPFSDSLLTECILVDQECVTNTKIKQEVNASSGCNTTPDSNTQDSDKIDYIESMDVCSNVEIESTEDSIVTGLNSGNAEDVDMTPGWRRKRNQKSKKSYIGTKDVLDQTLDKDIPLNKQNRRFPITARPVKRECVKKYERETFENGNERVYSTSSPRGRVYLLNDAAKLYEQAVKTEDKSTITKKPSYSRYPLISNFLTHKKKRSLLVLPRFELLKLARLGGKSSTNGFHHAAKNNTIWQYQCSRPLFRTCWSYRTSNATSLSSLALQLRILWSCLRWDDMIAKPPSTDGKHQVTTDTEIVTLELLKLRHSGRYGEKTSYLRRKVVIPLEMPKTVREVTSIRSGLRKRKRAESPQPTEPQITEEWVDEDKLELWEIKFMGEKQEKARLSAVTRSVASRQLEASGSNGSNTSTNGALGVAGRVQLAPKLSEDVKEKMEQQLKLQRAVHQQRKLVATGEITRSVTPVKGQVIGSRRVIVKNPDGTTRIIQQAVTQVSRTGGANTAAAAASPTVGGSTSTQSNPSTSTPHKVQIIRGPDGKVSVRGLNPGQQLVQMPDGKLHVLTTTTSSNSAGQGNKMKVPIKPASTSSSPAISSAQTTTNPVTPVIKQIAVKHVTKNSATQSIASSSRVALPLAQIKNKLLLAQQQQQSTSSSPATSSSPVQKIVSKVVNTSTSGQTLQQVFVQSGSKLVVGQNAQGQKVIISTSAAQQQGTSPVQQQQLVQSQPIQQSPQQISMTQVGNQPTQKVIQQIVNTSNVQQQIVVGGQRIILSPGQTIVTQRNVPQSQALQMVQQQIQTQQQQQQHHVVQPQQQFVVQSNQIVQSSPSAQTKLVKQLVVQQQSQQTIEEKTQITTTDSNETGTQQVLVPNSTLAQQLAQGKLQVATVNGQQVIVKPLGNNQAQIVAHIKHQGDGNAHIVTSNSATAVPQANPQTSPVKQQALPPQSPQQVVVQQQQIHQQSPTNFESGVTPITQQPVLTQAVQAPAQQQALSVEESLLQNQPPGTVIKCVTAQVLQTEHGPRIVLQGLVGNDFTAQQLQLVQTQVKQQLMKAQESNGKLGVLGPTKIYLAVQPENAVQSQPPPLTPVHQSAAHQQTNNIEIDADTLATTYEANSTIKDIAINNGDDQENSKCAETENSNITTNESFAGTSSLLEGSEHDEPTNLAGLDISETDLENKQNESFVVTRGYIQKSISNALKQGNLSPELEEKLVCMQKQQENANSTNEWETCSRGSVNEEALTPSRQTDDTEWKIRTSLRRPNAMTTSSQFNRILKKNRSKNDEVAELGEQKQSQLERHKELLKKNILRKRSLLERNLQSEIHEDVKTKVQRHVRPLSNASPDEQSENERSGEPNLDFKRTEVQNPRHGAGRPKKLTRKKEKLYCICRTPYDDTKFYVGCDLCSNWFHGDCVSITEEASKKLSEFICIDCKRARETQQLYCSCRQPYDESQFYICCDKCQDWFHGRCVGILQSEAEFIDEYVCPECQRKNDANAANMKKLTSNDVEELKNLIKQMQLHKSAWPFMEPVDPKEAPDYYKVIKEPMDLKRMEIKLESNTYTKLSEFIGDMTKIFDNCRYYNPKESSFYKCAEALESYFVQKIKNFRENVFDQRT

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
40	Phosphorylation	KYLSEGKSQPSTPSA CCCCCCCCCCCCCCC	59.17	22817900
46	Phosphorylation	KSQPSTPSASRGISP CCCCCCCCCCCCCCC	38.68	25749252
52	Phosphorylation	PSASRGISPQSDEGS CCCCCCCCCCCCCCC	21.51	19429919
55	Phosphorylation	SRGISPQSDEGSRSS CCCCCCCCCCCCCCC	40.80	19429919
59	Phosphorylation	SPQSDEGSRSSHNNH CCCCCCCCCCCCCCC	27.26	22817900
62	Phosphorylation	SDEGSRSSHNNHTNR CCCCCCCCCCCCCCC	29.17	22817900
83	Phosphorylation	KRGRGRKSAVQPNTS HCCCCCCCCCCCCCC	32.10	27626673
106	Phosphorylation	ESEYHYGSDFGDSEE CCCCCCCCCCCCCCC	23.70	19429919
111	Phosphorylation	YGSDFGDSEEDKSDN CCCCCCCCCCCCCCC	43.17	19429919
521	Phosphorylation	EKDGDAKSESQSIEG CCCCCCCCCHHCCCC	44.17	22668510
523	Phosphorylation	DGDAKSESQSIEGTK CCCCCCCHHCCCCCC	36.00	27794539
525	Phosphorylation	DAKSESQSIEGTKKQ CCCCCHHCCCCCCCH	31.70	22668510
543	Phosphorylation	KMVTRQKSNQLTNGT HEEEECCCCCCCCCE	22.82	19429919
1167	Acetylation	KKSYIGTKDVLDQTL CCCCCCCHHHHHHHH	40.40	21791702
1417	Phosphorylation	RKRKRAESPQPTEPQ HHHCCCCCCCCCCCC	28.05	19429919
1525	Phosphorylation	ATGEITRSVTPVKGQ HCCCCCCCCCCCCCE	22.99	22817900
1527	Phosphorylation	GEITRSVTPVKGQVI CCCCCCCCCCCCEEE	24.50	22817900
1542	Acetylation	GSRRVIVKNPDGTTR CCEEEEEECCCCCHH	52.19	21791702
1559	Phosphorylation	QQAVTQVSRTGGANT HHHHHHHHCCCCCCH	17.82	21082442
1572	Phosphorylation	NTAAAAASPTVGGST CHHHHHCCCCCCCCC	19.17	21082442
1666	Phosphorylation	QTTTNPVTPVIKQIA CCCCCCCCHHHHHHH	17.07	21082442
1722	Phosphorylation	SSPATSSSPVQKIVS CCCCCCCCHHHHHHH	28.94	19060867
1833	Phosphorylation	GGQRIILSPGQTIVT CCEEEEECCCCEEEE	19.31	22817900
1994	Phosphorylation	VPQANPQTSPVKQQA CCCCCCCCCCCCCCC	35.93	19429919
1995	Phosphorylation	PQANPQTSPVKQQAL CCCCCCCCCCCCCCC	23.58	19429919
2125 (in isoform 4)	Phosphorylation	-	28.42	21082442
2130 (in isoform 4)	Phosphorylation	-	4.86	21082442
2145	Phosphorylation	QSQPPPLTPVHQSAA CCCCCCCCCCCCCCC	29.10	19060867
2145 (in isoform 3)	Phosphorylation	-	29.10	21082442
2150 (in isoform 3)	Phosphorylation	-	16.73	21082442
2263	Phosphorylation	ALKQGNLSPELEEKL HHHHCCCCHHHHHHH	21.96	19429919
2337	Phosphorylation	RILKKNRSKNDEVAE HHHHHCCCCCHHHHH	45.25	22817900
2395	Phosphorylation	QRHVRPLSNASPDEQ HHHCCCCCCCCCCCC	32.79	19429919
2398	Phosphorylation	VRPLSNASPDEQSEN CCCCCCCCCCCCCCC	36.81	19429919
2403	Phosphorylation	NASPDEQSENERSGE CCCCCCCCCCCCCCC	40.10	19429919

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NU301_DROME !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NU301_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NU301_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ISWI_DROME	Iswi	physical	11583616
CAF1_DROME	Caf1	physical	11583616
IPYR_DROME	Nurf-38	physical	11583616
KEN_DROME	ken	physical	18334252
WASH1_DROME	wash	physical	27046080
ISWI_DROME	Iswi	physical	26549447
CP190_DROME	Cp190	physical	27046080

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteome analysis of Drosophila melanogaster embryos.";
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
J. Proteome Res. 7:1675-1682(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40; SER-52; SER-55;SER-59; SER-62; SER-1417; THR-1527; SER-2395; SER-2398 AND SER-2403,AND MASS SPECTROMETRY.
PubMed: 18327897