PSD10_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PSD10_HUMAN
• UniProt AC: O75832
• Protein Name: 26S proteasome non-ATPase regulatory subunit 10
• Gene Name: PSMD10
• Organism: Homo sapiens (Human).
• Sequence Length: 226
• Subcellular Localization: Cytoplasm . Nucleus .
• Protein Description: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.; Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export..
• Protein Sequence: MEGCVSNLMVCNLAYSGKLEELKESILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIYIENKEEKTPLQVAKGGLGLILKRMVEG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEGCVSNL -------CCCHHHCE	10.10	-
6	Phosphorylation	--MEGCVSNLMVCNL --CCCHHHCEEHHHH	28.46	23401153
15	Phosphorylation	LMVCNLAYSGKLEEL EEHHHHHHCCCHHHH	22.56	23401153
18	Ubiquitination	CNLAYSGKLEELKES HHHHHCCCHHHHHHH	46.95	22817900
23	Ubiquitination	SGKLEELKESILADK CCCHHHHHHHHHCCC	53.33	22817900
23	Ubiquitination	SGKLEELKESILADK CCCHHHHHHHHHCCC	53.33	21890473
23	Ubiquitination	SGKLEELKESILADK CCCHHHHHHHHHCCC	53.33	21890473
25	Phosphorylation	KLEELKESILADKSL CHHHHHHHHHCCCCH	22.70	29396449
30	Ubiquitination	KESILADKSLATRTD HHHHHCCCCHHCCCC	41.59	21890473
30	Ubiquitination	KESILADKSLATRTD HHHHHCCCCHHCCCC	41.59	23000965
30	Ubiquitination	KESILADKSLATRTD HHHHHCCCCHHCCCC	41.59	21890473
30	Acetylation	KESILADKSLATRTD HHHHHCCCCHHCCCC	41.59	25953088
30	2-Hydroxyisobutyrylation	KESILADKSLATRTD HHHHHCCCCHHCCCC	41.59	-
75	Phosphorylation	DKDDAGWSPLHIAAS CCCCCCCCHHHHHHH	19.37	20873877
90	Ubiquitination	AGRDEIVKALLGKGA CCCHHHHHHHHCCCC	38.69	23000965
90	Ubiquitination	AGRDEIVKALLGKGA CCCHHHHHHHHCCCC	38.69	21890473
90	Ubiquitination	AGRDEIVKALLGKGA CCCHHHHHHHHCCCC	38.69	21890473
90	2-Hydroxyisobutyrylation	AGRDEIVKALLGKGA CCCHHHHHHHHCCCC	38.69	-
95	Ubiquitination	IVKALLGKGAQVNAV HHHHHHCCCCCCEEE	51.55	23000965
112	Phosphorylation	NGCTPLHYAASKNRH CCCCHHHHHHCCCCC	16.14	28857561
115	Phosphorylation	TPLHYAASKNRHEIA CHHHHHHCCCCCHHE	23.45	26437602
116	Acetylation	PLHYAASKNRHEIAV HHHHHHCCCCCHHEE	53.96	25953088
116	Malonylation	PLHYAASKNRHEIAV HHHHHHCCCCCHHEE	53.96	26320211
116	Ubiquitination	PLHYAASKNRHEIAV HHHHHHCCCCCHHEE	53.96	32015554
138	Phosphorylation	NPDAKDHYEATAMHR CCCHHHHHHHHHHHH	19.86	24927040
138	Nitration	NPDAKDHYEATAMHR CCCHHHHHHHHHHHH	19.86	-
143	Sulfoxidation	DHYEATAMHRAAAKG HHHHHHHHHHHHHHC	1.59	30846556
149	Ubiquitination	AMHRAAAKGNLKMIH HHHHHHHHCCCEEEE	43.54	22817900
153	Ubiquitination	AAAKGNLKMIHILLY HHHHCCCEEEEEEEH	39.55	21906983
193	Phosphorylation	EEAKLLVSQGASIYI HHHHHHHHCCCCEEE	23.90	29396449
197	Phosphorylation	LLVSQGASIYIENKE HHHHCCCCEEEECCC	23.81	29396449
199	Phosphorylation	VSQGASIYIENKEEK HHCCCCEEEECCCCC	10.33	29396449
207	Phosphorylation	IENKEEKTPLQVAKG EECCCCCCCCCHHCC	32.29	20068231
213	Ubiquitination	KTPLQVAKGGLGLIL CCCCCHHCCCHHHHH	55.44	21890473
213	Ubiquitination	KTPLQVAKGGLGLIL CCCCCHHCCCHHHHH	55.44	22817900
221	2-Hydroxyisobutyrylation	GGLGLILKRMVEG-- CCHHHHHHHHHCC--	31.30	-
221	Ubiquitination	GGLGLILKRMVEG-- CCHHHHHHHHHCC--	31.30	21906983
221	Ubiquitination	GGLGLILKRMVEG-- CCHHHHHHHHHCC--	31.30	21890473
221	Acetylation	GGLGLILKRMVEG-- CCHHHHHHHHHCC--	31.30	25953088

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PSD10_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PSD10_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PSD10_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PSMD1_HUMAN	PSMD1	physical	17353931
PSMD2_HUMAN	PSMD2	physical	17353931
PSD11_HUMAN	PSMD11	physical	17353931
PRS8_HUMAN	PSMC5	physical	17353931
PRS6B_HUMAN	PSMC4	physical	17353931
PRS4_HUMAN	PSMC1	physical	17353931
DOK2_HUMAN	DOK2	physical	17353931
PSMD3_HUMAN	PSMD3	physical	17353931
PRS6A_HUMAN	PSMC3	physical	17353931
PRS7_HUMAN	PSMC2	physical	17353931
PSMD7_HUMAN	PSMD7	physical	17353931
PRS10_HUMAN	PSMC6	physical	17353931
PSMD6_HUMAN	PSMD6	physical	17353931
PSD12_HUMAN	PSMD12	physical	17353931
PAAF1_HUMAN	PAAF1	physical	17353931
PSMD4_HUMAN	PSMD4	physical	17353931
PRS6B_HUMAN	PSMC4	physical	11779854
MDM2_HUMAN	MDM2	physical	18332869
PRS6B_HUMAN	PSMC4	physical	19412159
MDM2_HUMAN	MDM2	physical	16023600
TF65_HUMAN	RELA	physical	17904523
NFKB1_HUMAN	NFKB1	physical	17904523
WWP2_HUMAN	WWP2	physical	22387393
PRS6B_HUMAN	PSMC4	physical	19490896
PRS8_HUMAN	PSMC5	physical	19490896
RB_HUMAN	RB1	physical	10613832
CDK4_HUMAN	CDK4	physical	11900540
CCND2_HUMAN	CCND2	physical	11900540
TF65_HUMAN	RELA	physical	18040287
GDIR1_HUMAN	ARHGDIA	physical	20628200
RHOA_HUMAN	RHOA	physical	20628200
MAGA4_HUMAN	MAGEA4	physical	12525503
PRS6B_HUMAN	PSMC4	physical	12525503
RB_HUMAN	RB1	physical	12525503
PSD11_HUMAN	PSMD11	physical	22939629
PSD12_HUMAN	PSMD12	physical	22939629
PSMD1_HUMAN	PSMD1	physical	22939629
PSMD3_HUMAN	PSMD3	physical	22939629
PSMD7_HUMAN	PSMD7	physical	22939629
PSDE_HUMAN	PSMD14	physical	22939629
PSMD2_HUMAN	PSMD2	physical	22939629
PSMD4_HUMAN	PSMD4	physical	22939629
PSD13_HUMAN	PSMD13	physical	22939629
PSMD6_HUMAN	PSMD6	physical	22939629
PSMD8_HUMAN	PSMD8	physical	22939629
OARD1_HUMAN	OARD1	physical	22863883
LMNA_HUMAN	LMNA	physical	22863883
KPRB_HUMAN	PRPSAP2	physical	22863883
RU2B_HUMAN	SNRPB2	physical	22863883
SQSTM_HUMAN	SQSTM1	physical	22863883
HSP74_HUMAN	HSPA4	physical	24338975
HS90A_HUMAN	HSP90AA1	physical	24338975
CLIC1_HUMAN	CLIC1	physical	24338975
GRSF1_HUMAN	GRSF1	physical	24338975
DDAH1_HUMAN	DDAH1	physical	24338975
MP2K1_HUMAN	MAP2K1	physical	24338975
NCK2_HUMAN	NCK2	physical	24338975
PAAF1_HUMAN	PAAF1	physical	26344197
PRS7_HUMAN	PSMC2	physical	26344197
PRS6A_HUMAN	PSMC3	physical	26344197
PSD11_HUMAN	PSMD11	physical	26344197
PSD13_HUMAN	PSMD13	physical	26344197
PSDE_HUMAN	PSMD14	physical	26344197
PSMD2_HUMAN	PSMD2	physical	26344197
PSMD3_HUMAN	PSMD3	physical	26344197
PSMD7_HUMAN	PSMD7	physical	26344197
EP300_HUMAN	EP300	physical	25400040
HIF1A_HUMAN	HIF1A	physical	26364616
KEAP1_HUMAN	KEAP1	physical	27091842

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Mass spectrometric characterization of the affinity-purified human26S proteasome complex.";
Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L.;
Biochemistry 46:3553-3565(2007).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
PubMed: 17323924