| UniProt ID | CTIP_HUMAN | |
|---|---|---|
| UniProt AC | Q99708 | |
| Protein Name | DNA endonuclease RBBP8 | |
| Gene Name | RBBP8 | |
| Organism | Homo sapiens (Human). | |
| Sequence Length | 897 | |
| Subcellular Localization | Nucleus . Chromosome . Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, PubMed:25349192). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604). | |
| Protein Description | Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. [PubMed: 10764811] | |
| Protein Sequence | MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGLQVKVTKLKQERILDAQRLEEFFTKNQQLREQQKVLHETIKVLEDRLRAGLCDRCAVTEEHMRKKQQEFENIRQQNLKLITELMNERNTLQEENKKLSEQLQQKIENDQQHQAAELECEEDVIPDSPITAFSFSGVNRLRRKENPHVRYIEQTHTKLEHSVCANEMRKVSKSSTHPQHNPNENEILVADTYDQSQSPMAKAHGTSSYTPDKSSFNLATVVAETLGLGVQEESETQGPMSPLGDELYHCLEGNHKKQPFEESTRNTEDSLRFSDSTSKTPPQEELPTRVSSPVFGATSSIKSGLDLNTSLSPSLLQPGKKKHLKTLPFSNTCISRLEKTRSKSEDSALFTHHSLGSEVNKIIIQSSNKQILINKNISESLGEQNRTEYGKDSNTDKHLEPLKSLGGRTSKRKKTEEESEHEVSCPQASFDKENAFPFPMDNQFSMNGDCVMDKPLDLSDRFSAIQRQEKSQGSETSKNKFRQVTLYEALKTIPKGFSSSRKASDGNCTLPKDSPGEPCSQECIILQPLNKCSPDNKPSLQIKEENAVFKIPLRPRESLETENVLDDIKSAGSHEPIKIQTRSDHGGCELASVLQLNPCRTGKIKSLQNNQDVSFENIQWSIDPGADLSQYKMDVTVIDTKDGSQSKLGGETVDMDCTLVSETVLLKMKKQEQKGEKSSNEERKMNDSLEDMFDRTTHEEYESCLADSFSQAADEEEELSTATKKLHTHGDKQDKVKQKAFVEPYFKGDERETSLQNFPHIEVVRKKEERRKLLGHTCKECEIYYADMPAEEREKKLASCSRHRFRYIPPNTPENFWEVGFPSTQTCMERGYIKEDLDPCPRPKRRQPYNAIFSPKGKEQKT | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 4 | Phosphorylation | ----MNISGSSCGSP ----CCCCCCCCCCC | 26074081 | ||
| 6 | Phosphorylation | --MNISGSSCGSPNS --CCCCCCCCCCCCC | 26074081 | ||
| 7 | Phosphorylation | -MNISGSSCGSPNSA -CCCCCCCCCCCCCC | 23401153 | ||
| 10 | Phosphorylation | ISGSSCGSPNSADTS CCCCCCCCCCCCCCC | 25159151 | ||
| 13 | Phosphorylation | SSCGSPNSADTSSDF CCCCCCCCCCCCHHH | 28985074 | ||
| 16 | Phosphorylation | GSPNSADTSSDFKDL CCCCCCCCCHHHHHH | 26074081 | ||
| 17 | Phosphorylation | SPNSADTSSDFKDLW CCCCCCCCHHHHHHH | 26074081 | ||
| 18 | Phosphorylation | PNSADTSSDFKDLWT CCCCCCCHHHHHHHH | 26074081 | ||
| 25 | Phosphorylation | SDFKDLWTKLKECHD HHHHHHHHHHHHHHH | 26074081 | ||
| 62 | Sumoylation | RLEEFFTKNQQLREQ HHHHHHHHHHHHHHH | 28112733 | ||
| 62 | Ubiquitination | RLEEFFTKNQQLREQ HHHHHHHHHHHHHHH | - | ||
| 115 | Sumoylation | NIRQQNLKLITELMN HHHHHHHHHHHHHHH | - | ||
| 115 | Sumoylation | NIRQQNLKLITELMN HHHHHHHHHHHHHHH | 28112733 | ||
| 126 | Phosphorylation | ELMNERNTLQEENKK HHHHHHHHHHHHHHH | - | ||
| 163 | Phosphorylation | EEDVIPDSPITAFSF CCCCCCCCCCEEEEE | 30266825 | ||
| 166 | Phosphorylation | VIPDSPITAFSFSGV CCCCCCCEEEEECCH | 30266825 | ||
| 169 | Phosphorylation | DSPITAFSFSGVNRL CCCCEEEEECCHHHH | 30266825 | ||
| 171 | Phosphorylation | PITAFSFSGVNRLRR CCEEEEECCHHHHHC | 27251275 | ||
| 193 | Sumoylation | YIEQTHTKLEHSVCA HEHHHCCHHHHHHHH | 28112733 | ||
| 227 | Phosphorylation | NEILVADTYDQSQSP CCEEEEECCCCCCCC | 30576142 | ||
| 228 | Phosphorylation | EILVADTYDQSQSPM CEEEEECCCCCCCCC | 30576142 | ||
| 231 | Phosphorylation | VADTYDQSQSPMAKA EEECCCCCCCCCHHH | 30576142 | ||
| 233 | Phosphorylation | DTYDQSQSPMAKAHG ECCCCCCCCCHHHCC | 23401153 | ||
| 241 | Phosphorylation | PMAKAHGTSSYTPDK CCHHHCCCCCCCCCC | 22115753 | ||
| 242 | Phosphorylation | MAKAHGTSSYTPDKS CHHHCCCCCCCCCCH | 29396449 | ||
| 243 | Phosphorylation | AKAHGTSSYTPDKSS HHHCCCCCCCCCCHH | 25159151 | ||
| 244 | Phosphorylation | KAHGTSSYTPDKSSF HHCCCCCCCCCCHHC | 27251275 | ||
| 245 | Phosphorylation | AHGTSSYTPDKSSFN HCCCCCCCCCCHHCC | 25159151 | ||
| 249 | Phosphorylation | SSYTPDKSSFNLATV CCCCCCCHHCCHHHH | 25332170 | ||
| 269 | Phosphorylation | GLGVQEESETQGPMS CCCCCCCCCCCCCCC | 27251275 | ||
| 271 | Phosphorylation | GVQEESETQGPMSPL CCCCCCCCCCCCCCC | 27251275 | ||
| 276 | Phosphorylation | SETQGPMSPLGDELY CCCCCCCCCCHHHHH | 26074081 | ||
| 302 | Phosphorylation | FEESTRNTEDSLRFS CCHHCCCCHHHCCCC | 20873877 | ||
| 305 | Phosphorylation | STRNTEDSLRFSDST HCCCCHHHCCCCCCC | 20873877 | ||
| 309 | Phosphorylation | TEDSLRFSDSTSKTP CHHHCCCCCCCCCCC | 29255136 | ||
| 311 | Phosphorylation | DSLRFSDSTSKTPPQ HHCCCCCCCCCCCCH | 29255136 | ||
| 312 | Phosphorylation | SLRFSDSTSKTPPQE HCCCCCCCCCCCCHH | 29255136 | ||
| 313 | Phosphorylation | LRFSDSTSKTPPQEE CCCCCCCCCCCCHHH | 29255136 | ||
| 314 | Ubiquitination | RFSDSTSKTPPQEEL CCCCCCCCCCCHHHC | - | ||
| 315 | Phosphorylation | FSDSTSKTPPQEELP CCCCCCCCCCHHHCC | 23401153 | ||
| 323 | Phosphorylation | PPQEELPTRVSSPVF CCHHHCCCCCCCCCC | 23401153 | ||
| 326 | Phosphorylation | EELPTRVSSPVFGAT HHCCCCCCCCCCCCC | 23927012 | ||
| 327 | Phosphorylation | ELPTRVSSPVFGATS HCCCCCCCCCCCCCC | 25159151 | ||
| 333 | Phosphorylation | SSPVFGATSSIKSGL CCCCCCCCCCCCCCC | 23927012 | ||
| 334 | Phosphorylation | SPVFGATSSIKSGLD CCCCCCCCCCCCCCC | 23927012 | ||
| 335 | Phosphorylation | PVFGATSSIKSGLDL CCCCCCCCCCCCCCC | 23927012 | ||
| 338 | Phosphorylation | GATSSIKSGLDLNTS CCCCCCCCCCCCCCC | 22199227 | ||
| 344 | Phosphorylation | KSGLDLNTSLSPSLL CCCCCCCCCCCHHHC | 30266825 | ||
| 345 | Phosphorylation | SGLDLNTSLSPSLLQ CCCCCCCCCCHHHCC | 30266825 | ||
| 347 | Phosphorylation | LDLNTSLSPSLLQPG CCCCCCCCHHHCCCC | 30266825 | ||
| 349 | Phosphorylation | LNTSLSPSLLQPGKK CCCCCCHHHCCCCCC | 30266825 | ||
| 360 | Sumoylation | PGKKKHLKTLPFSNT CCCCCCCCCCCCCHH | 28112733 | ||
| 360 | Ubiquitination | PGKKKHLKTLPFSNT CCCCCCCCCCCCCHH | - | ||
| 361 | Phosphorylation | GKKKHLKTLPFSNTC CCCCCCCCCCCCHHH | - | ||
| 375 | Phosphorylation | CISRLEKTRSKSEDS HHHHHHHHCCCCCCC | 29632367 | ||
| 377 | Phosphorylation | SRLEKTRSKSEDSAL HHHHHHCCCCCCCHH | 29632367 | ||
| 378 | Sumoylation | RLEKTRSKSEDSALF HHHHHCCCCCCCHHH | - | ||
| 378 | Sumoylation | RLEKTRSKSEDSALF HHHHHCCCCCCCHHH | 28112733 | ||
| 378 | Ubiquitination | RLEKTRSKSEDSALF HHHHHCCCCCCCHHH | - | ||
| 379 | Phosphorylation | LEKTRSKSEDSALFT HHHHCCCCCCCHHHC | 25159151 | ||
| 382 | Phosphorylation | TRSKSEDSALFTHHS HCCCCCCCHHHCHHC | 29632367 | ||
| 386 | Phosphorylation | SEDSALFTHHSLGSE CCCCHHHCHHCCCHH | 29632367 | ||
| 389 | Phosphorylation | SALFTHHSLGSEVNK CHHHCHHCCCHHHHE | 29632367 | ||
| 392 | Phosphorylation | FTHHSLGSEVNKIII HCHHCCCHHHHEEEE | 29978859 | ||
| 396 | Sumoylation | SLGSEVNKIIIQSSN CCCHHHHEEEEECCC | 28112733 | ||
| 404 | Sumoylation | IIIQSSNKQILINKN EEEECCCCEEEECCC | 28112733 | ||
| 410 | Sumoylation | NKQILINKNISESLG CCEEEECCCHHHHHC | 28112733 | ||
| 410 | Ubiquitination | NKQILINKNISESLG CCEEEECCCHHHHHC | - | ||
| 422 | Phosphorylation | SLGEQNRTEYGKDSN HHCCCCCHHHCCCCC | - | ||
| 424 | Phosphorylation | GEQNRTEYGKDSNTD CCCCCHHHCCCCCCH | - | ||
| 428 | Phosphorylation | RTEYGKDSNTDKHLE CHHHCCCCCCHHHHH | - | ||
| 432 | Acetylation | GKDSNTDKHLEPLKS CCCCCCHHHHHHHHH | 20829486 | ||
| 438 | Sumoylation | DKHLEPLKSLGGRTS HHHHHHHHHCCCCCC | - | ||
| 438 | Sumoylation | DKHLEPLKSLGGRTS HHHHHHHHHCCCCCC | 28112733 | ||
| 438 | Ubiquitination | DKHLEPLKSLGGRTS HHHHHHHHHCCCCCC | - | ||
| 439 | Phosphorylation | KHLEPLKSLGGRTSK HHHHHHHHCCCCCCC | 24719451 | ||
| 449 | Sumoylation | GRTSKRKKTEEESEH CCCCCCCCCHHHHCC | - | ||
| 449 | Sumoylation | GRTSKRKKTEEESEH CCCCCCCCCHHHHCC | 28112733 | ||
| 450 | Phosphorylation | RTSKRKKTEEESEHE CCCCCCCCHHHHCCC | 27251275 | ||
| 454 | Phosphorylation | RKKTEEESEHEVSCP CCCCHHHHCCCCCCC | 27251275 | ||
| 467 | Ubiquitination | CPQASFDKENAFPFP CCCCCCCCCCCCCCC | 27561354 | ||
| 513 | Acetylation | SQGSETSKNKFRQVT HCCCCCCHHHHHHHH | 67251203 | ||
| 515 | Acetylation | GSETSKNKFRQVTLY CCCCCHHHHHHHHHH | 67251201 | ||
| 522 | Phosphorylation | KFRQVTLYEALKTIP HHHHHHHHHHHHHCC | - | ||
| 526 | Acetylation | VTLYEALKTIPKGFS HHHHHHHHHCCCCCC | 20829486 | ||
| 526 | Sumoylation | VTLYEALKTIPKGFS HHHHHHHHHCCCCCC | 28112733 | ||
| 526 | Ubiquitination | VTLYEALKTIPKGFS HHHHHHHHHCCCCCC | 20829486 | ||
| 527 | O-linked_Glycosylation | TLYEALKTIPKGFSS HHHHHHHHCCCCCCC | 31492838 | ||
| 530 | Sumoylation | EALKTIPKGFSSSRK HHHHHCCCCCCCCCC | 28112733 | ||
| 530 | Ubiquitination | EALKTIPKGFSSSRK HHHHHCCCCCCCCCC | - | ||
| 533 | Phosphorylation | KTIPKGFSSSRKASD HHCCCCCCCCCCCCC | 23882029 | ||
| 534 | Phosphorylation | TIPKGFSSSRKASDG HCCCCCCCCCCCCCC | 23882029 | ||
| 535 | Phosphorylation | IPKGFSSSRKASDGN CCCCCCCCCCCCCCC | 23882029 | ||
| 539 | Phosphorylation | FSSSRKASDGNCTLP CCCCCCCCCCCCCCC | 24247654 | ||
| 544 | Phosphorylation | KASDGNCTLPKDSPG CCCCCCCCCCCCCCC | 23882029 | ||
| 549 | Phosphorylation | NCTLPKDSPGEPCSQ CCCCCCCCCCCCCCC | 25159151 | ||
| 555 | Phosphorylation | DSPGEPCSQECIILQ CCCCCCCCCEEEEEE | 30576142 | ||
| 568 | Phosphorylation | LQPLNKCSPDNKPSL EEECHHCCCCCCCCC | 20873877 | ||
| 572 | Sumoylation | NKCSPDNKPSLQIKE HHCCCCCCCCCEEEC | 28112733 | ||
| 574 | Phosphorylation | CSPDNKPSLQIKEEN CCCCCCCCCEEECCC | 26074081 | ||
| 578 | Sumoylation | NKPSLQIKEENAVFK CCCCCEEECCCEEEE | - | ||
| 578 | Sumoylation | NKPSLQIKEENAVFK CCCCCEEECCCEEEE | 28112733 | ||
| 585 | Ubiquitination | KEENAVFKIPLRPRE ECCCEEEEEECCCHH | - | ||
| 593 | Phosphorylation | IPLRPRESLETENVL EECCCHHHCCCCCHH | 28355574 | ||
| 596 | Phosphorylation | RPRESLETENVLDDI CCHHHCCCCCHHHHH | 27251275 | ||
| 604 | Sumoylation | ENVLDDIKSAGSHEP CCHHHHHHHCCCCCC | - | ||
| 604 | Acetylation | ENVLDDIKSAGSHEP CCHHHHHHHCCCCCC | 20829486 | ||
| 604 | Sumoylation | ENVLDDIKSAGSHEP CCHHHHHHHCCCCCC | 20829486 | ||
| 604 | Ubiquitination | ENVLDDIKSAGSHEP CCHHHHHHHCCCCCC | 20829486 | ||
| 604 (in isoform 2) | Ubiquitination | - | - | ||
| 613 | Sumoylation | AGSHEPIKIQTRSDH CCCCCCEEEEECCCC | 28112733 | ||
| 613 | Ubiquitination | AGSHEPIKIQTRSDH CCCCCCEEEEECCCC | - | ||
| 638 | Sumoylation | LNPCRTGKIKSLQNN CCCCCCCCCCCCCCC | 28112733 | ||
| 640 | Sumoylation | PCRTGKIKSLQNNQD CCCCCCCCCCCCCCC | 28112733 | ||
| 640 | Ubiquitination | PCRTGKIKSLQNNQD CCCCCCCCCCCCCCC | - | ||
| 649 | Phosphorylation | LQNNQDVSFENIQWS CCCCCCCCEEEEEEE | 27251275 | ||
| 664 | Phosphorylation | IDPGADLSQYKMDVT ECCCCCHHHCEEEEE | 11689934 | ||
| 676 | Sumoylation | DVTVIDTKDGSQSKL EEEEEECCCCCCCCC | 28112733 | ||
| 679 | Phosphorylation | VIDTKDGSQSKLGGE EEECCCCCCCCCCCE | 17525332 | ||
| 693 | Phosphorylation | ETVDMDCTLVSETVL EECCCCCEEEEHHHH | - | ||
| 714 | Phosphorylation | EQKGEKSSNEERKMN HHHCCCCCHHHHHHH | 28787133 | ||
| 719 | Sumoylation | KSSNEERKMNDSLED CCCHHHHHHHHHHHH | 28112733 | ||
| 723 | Phosphorylation | EERKMNDSLEDMFDR HHHHHHHHHHHHHHC | 25159151 | ||
| 731 | Phosphorylation | LEDMFDRTTHEEYES HHHHHHCCHHHHHHH | - | ||
| 743 | Phosphorylation | YESCLADSFSQAADE HHHHHHHHHHHHCCH | 30576142 | ||
| 745 | Phosphorylation | SCLADSFSQAADEEE HHHHHHHHHHCCHHH | 11689934 | ||
| 755 | Phosphorylation | ADEEEELSTATKKLH CCHHHHHHHHHHHHH | 30576142 | ||
| 756 | Phosphorylation | DEEEELSTATKKLHT CHHHHHHHHHHHHHH | 30576142 | ||
| 760 | Ubiquitination | ELSTATKKLHTHGDK HHHHHHHHHHHCCCC | - | ||
| 774 | Sumoylation | KQDKVKQKAFVEPYF CCHHHHHHHHCCCCC | - | ||
| 774 | Sumoylation | KQDKVKQKAFVEPYF CCHHHHHHHHCCCCC | - | ||
| 782 | Sumoylation | AFVEPYFKGDERETS HHCCCCCCCCCCCHH | 28112733 | ||
| 782 | Ubiquitination | AFVEPYFKGDERETS HHCCCCCCCCCCCHH | - | ||
| 788 | Phosphorylation | FKGDERETSLQNFPH CCCCCCCHHHHCCCC | 20873877 | ||
| 789 | Phosphorylation | KGDERETSLQNFPHI CCCCCCHHHHCCCCE | 20873877 | ||
| 836 | Phosphorylation | EKKLASCSRHRFRYI HHHHHHCCCCCCCCC | 23532336 | ||
| 847 | Phosphorylation | FRYIPPNTPENFWEV CCCCCCCCCCCCCCC | 26502057 | ||
| 859 | Phosphorylation | WEVGFPSTQTCMERG CCCCCCCHHHHHHCC | - | ||
| 869 | Sumoylation | CMERGYIKEDLDPCP HHHCCCCCCCCCCCC | - | ||
| 869 | Sumoylation | CMERGYIKEDLDPCP HHHCCCCCCCCCCCC | 28112733 | ||
| 884 | Phosphorylation | RPKRRQPYNAIFSPK CCCCCCCCCCCCCCC | 30576142 | ||
| 889 | Phosphorylation | QPYNAIFSPKGKEQK CCCCCCCCCCCCCCC | 28985074 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
| 126 | T | Phosphorylation | Kinase | PKM | P14618 | PSP |
| 315 | T | Phosphorylation | Kinase | CDK2 | P24941 | Uniprot |
| 327 | S | Phosphorylation | Kinase | AURA | O14965 | PSP |
| 327 | S | Phosphorylation | Kinase | CDK1 | P06493 | PSP |
| 593 | S | Phosphorylation | Kinase | AURA | O14965 | PSP |
| 664 | S | Phosphorylation | Kinase | ATM | Q13315 | Uniprot |
| 693 | T | Phosphorylation | Kinase | PLK1 | P53350 | PSP |
| 723 | S | Phosphorylation | Kinase | PLK1 | P53350 | PSP |
| 731 | T | Phosphorylation | Kinase | PLK1 | P53350 | PSP |
| 745 | S | Phosphorylation | Kinase | ATM | Q13315 | Uniprot |
| 847 | T | Phosphorylation | Kinase | CDK1 | P06493 | Uniprot |
| 847 | T | Phosphorylation | Kinase | CDK2 | P24941 | PSP |
| 859 | T | Phosphorylation | Kinase | ATR | Q13535 | PSP |
| - | K | Ubiquitination | E3 ubiquitin ligase | BRCA1 | P38398 | PMID:16818604 |
| - | K | Ubiquitination | E3 ubiquitin ligase | SIAH1 | Q8IUQ4 | PMID:14654780 |
| - | K | Ubiquitination | E3 ubiquitin ligase | RNF138 | Q8WVD3 | PMID:22199232 |
| - | K | Ubiquitination | E3 ubiquitin ligase | FZR1 | Q9UM11 | PMID:22199232 |
| - | K | Ubiquitination | E3 ubiquitin ligase | KLHL15 | Q96M94 | PMID:27561354 |
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of CTIP_HUMAN !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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