dbPTM

CRCM_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CRCM_HUMAN
UniProt AC	P23508
Protein Name	Colorectal mutant cancer protein
Gene Name	MCC
Organism	Homo sapiens (Human).
Sequence Length	829
Subcellular Localization	Cell membrane. Cell projection, lamellipodium. Nucleus . Cytoplasm . Colocalizes with actin at the leading edge of polarized cells.
Protein Description	Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation. [PubMed: 18591935]
Protein Sequence	MNSGVAMKYGNDSSAELSELHSAALASLKGDIVELNKRLQQTERERDLLEKKLAKAQCEQSHLMREHEDVQERTTLRYEERITELHSVIAELNKKIDRLQGTTIREEDEYSELRSELSQSQHEVNEDSRSMDQDQTSVSIPENQSTMVTADMDNCSDLNSELQRVLTGLENVVCGRKKSSCSLSVAEVDKHIEQLTTASEHCDLAIKTVEEIEGVLGRDLYPNLAEERSRWEKELAGLREENESLTAMLCSKEEELNRTKATMNAIREERDRLRRRVRELQTRLQSVQATGPSSPGRLTSTNRPINPSTGELSTSSSSNDIPIAKIAERVKLSKTRSESSSSDRPVLGSEISSIGVSSSVAEHLAHSLQDCSNIQEIFQTLYSHGSAISESKIREFEVETERLNSRIEHLKSQNDLLTITLEECKSNAERMSMLVGKYESNATALRLALQYSEQCIEAYELLLALAESEQSLILGQFRAAGVGSSPGDQSGDENITQMLKRAHDCRKTAENAAKALLMKLDGSCGGAFAVAGCSVQPWESLSSNSHTSTTSSTASSCDTEFTKEDEQRLKDYIQQLKNDRAAVKLTMLELESIHIDPLSYDVKPRGDSQRLDLENAVLMQELMAMKEEMAELKAQLYLLEKEKKALELKLSTREAQEQAYLVHIEHLKSEVEEQKEQRMRSLSSTSSGSKDKPGKECADAASPALSLAELRTTCSENELAAEFTNAIRREKKLKARVQELVSALERLTKSSEIRHQQSAEFVNDLKRANSNLVAAYEKAKKKHQNKLKKLESQMMAMVERHETQVRMLKQRIALLEEENSRPHTNETSL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
12 (in isoform 2)	Phosphorylation	-	39.34	24043423
13 (in isoform 2)	Phosphorylation	-	35.13	24043423
14 (in isoform 2)	Phosphorylation	-	28.73	24043423
15 (in isoform 2)	Phosphorylation	-	26.21	24043423
22 (in isoform 2)	Phosphorylation	-	28.06	24043423
24 (in isoform 2)	Phosphorylation	-	13.48	24043423
25 (in isoform 2)	Phosphorylation	-	3.13	24043423
26 (in isoform 2)	Phosphorylation	-	14.21	24043423
27 (in isoform 2)	Phosphorylation	-	31.31	24043423
28 (in isoform 2)	Phosphorylation	-	6.30	24043423
29 (in isoform 2)	Phosphorylation	-	52.63	24043423
31 (in isoform 2)	Phosphorylation	-	39.92	24043423
32 (in isoform 2)	Phosphorylation	-	3.37	24043423
33 (in isoform 2)	Phosphorylation	-	9.00	24043423
34 (in isoform 2)	Phosphorylation	-	47.13	24043423
58	S-nitrosylation	KKLAKAQCEQSHLMR HHHHHHHHHHHHHHH	6.50	24105792
89 (in isoform 2)	Phosphorylation	-	2.50	27067055
103	Phosphorylation	IDRLQGTTIREEDEY HHHHCCCCCCCHHHH	25.51	24043423
110	Phosphorylation	TIREEDEYSELRSEL CCCCHHHHHHHHHHH	21.55	27732954
111	Phosphorylation	IREEDEYSELRSELS CCCHHHHHHHHHHHH	28.27	29255136
115	Phosphorylation	DEYSELRSELSQSQH HHHHHHHHHHHHHHH	56.36	23663014
118	Phosphorylation	SELRSELSQSQHEVN HHHHHHHHHHHHHHC	24.59	29255136
120	Phosphorylation	LRSELSQSQHEVNED HHHHHHHHHHHHCCC	30.08	29255136
128	Phosphorylation	QHEVNEDSRSMDQDQ HHHHCCCHHCCCCCC	21.78	23927012
129	Phosphorylation	HEVNEDSRSMDQDQT HHHCCCHHCCCCCCC	48.61	27251275
129 (in isoform 2)	Phosphorylation	-	48.61	25884760
132 (in isoform 2)	Phosphorylation	-	50.59	23090842
133 (in isoform 2)	Phosphorylation	-	35.75	28348404
134 (in isoform 2)	Phosphorylation	-	49.84	28348404
159	Phosphorylation	MDNCSDLNSELQRVL CCCCHHHHHHHHHHH	38.13	27251275
159 (in isoform 2)	Phosphorylation	-	38.13	25849741
160	Phosphorylation	DNCSDLNSELQRVLT CCCHHHHHHHHHHHH	47.16	24275569
164 (in isoform 2)	Phosphorylation	-	37.73	30266825
166 (in isoform 2)	Phosphorylation	-	3.24	30266825
179	Phosphorylation	VVCGRKKSSCSLSVA HHCCCCCCCCCCCHH	39.34	28655764
182	Phosphorylation	GRKKSSCSLSVAEVD CCCCCCCCCCHHHHH	26.15	28655764
184	Phosphorylation	KKSSCSLSVAEVDKH CCCCCCCCHHHHHHH	11.73	19664994
196	Phosphorylation	DKHIEQLTTASEHCD HHHHHHHHHHHHHCC	21.73	29255136
197	Phosphorylation	KHIEQLTTASEHCDL HHHHHHHHHHHHCCE	36.46	29255136
199	Phosphorylation	IEQLTTASEHCDLAI HHHHHHHHHHCCEEH	26.27	29255136
244	Phosphorylation	GLREENESLTAMLCS HHHHHCHHHHHHHCC	42.47	26074081
246	Phosphorylation	REENESLTAMLCSKE HHHCHHHHHHHCCCH	21.21	26074081
251	Phosphorylation	SLTAMLCSKEEELNR HHHHHHCCCHHHHHH	38.86	26074081
260	Methylation	EEELNRTKATMNAIR HHHHHHHHHHHHHHH	38.33	23644510
286	Phosphorylation	ELQTRLQSVQATGPS HHHHHHHHHHCCCCC	22.31	23403867
290	Phosphorylation	RLQSVQATGPSSPGR HHHHHHCCCCCCCCC	32.31	22167270
293	Phosphorylation	SVQATGPSSPGRLTS HHHCCCCCCCCCCCC	50.97	30266825
294	Phosphorylation	VQATGPSSPGRLTST HHCCCCCCCCCCCCC	34.21	30266825
299	Phosphorylation	PSSPGRLTSTNRPIN CCCCCCCCCCCCCCC	31.95	28857561
300	Phosphorylation	SSPGRLTSTNRPINP CCCCCCCCCCCCCCC	28.42	28857561
301	Phosphorylation	SPGRLTSTNRPINPS CCCCCCCCCCCCCCC	29.97	28857561
308	Phosphorylation	TNRPINPSTGELSTS CCCCCCCCCCCCCCC	44.55	24719451
309	Phosphorylation	NRPINPSTGELSTSS CCCCCCCCCCCCCCC	35.61	28857561
310	Phosphorylation	RPINPSTGELSTSSS CCCCCCCCCCCCCCC	37.88	24719451
313	Phosphorylation	NPSTGELSTSSSSND CCCCCCCCCCCCCCC	23.18	28857561
314	Phosphorylation	PSTGELSTSSSSNDI CCCCCCCCCCCCCCC	45.30	28857561
315	Phosphorylation	STGELSTSSSSNDIP CCCCCCCCCCCCCCC	25.50	28857561
316	Phosphorylation	TGELSTSSSSNDIPI CCCCCCCCCCCCCCH	37.56	28857561
317	Phosphorylation	GELSTSSSSNDIPIA CCCCCCCCCCCCCHH	32.69	28857561
318	Phosphorylation	ELSTSSSSNDIPIAK CCCCCCCCCCCCHHH	40.23	28857561
369	Phosphorylation	EHLAHSLQDCSNIQE HHHHHHHHHCCCHHH	53.32	24719451
372	Phosphorylation	AHSLQDCSNIQEIFQ HHHHHHCCCHHHHHH	45.30	27251275
389	Phosphorylation	YSHGSAISESKIREF HHCCCCCCHHHHCCH	35.22	27251275
405	Phosphorylation	VETERLNSRIEHLKS HCHHHHHHHHHHHHH	38.95	-
412	Phosphorylation	SRIEHLKSQNDLLTI HHHHHHHHCCCEEEE	40.34	19690332
420	Phosphorylation	QNDLLTITLEECKSN CCCEEEEEHHHHHHH	23.92	19690332
426	Phosphorylation	ITLEECKSNAERMSM EEHHHHHHHHHHHHH	53.59	26074081
432	Phosphorylation	KSNAERMSMLVGKYE HHHHHHHHHHHHHHH	18.39	26074081
438	Phosphorylation	MSMLVGKYESNATAL HHHHHHHHHHHHHHH	20.75	26074081
483	Phosphorylation	QFRAAGVGSSPGDQS CHHHCCCCCCCCCCC	22.84	24719451
484	Phosphorylation	FRAAGVGSSPGDQSG HHHCCCCCCCCCCCC	30.84	19664995
485	Phosphorylation	RAAGVGSSPGDQSGD HHCCCCCCCCCCCCC	27.29	22199227
490	Phosphorylation	GSSPGDQSGDENITQ CCCCCCCCCCHHHHH	53.61	29396449
496	Phosphorylation	QSGDENITQMLKRAH CCCCHHHHHHHHHHH	21.36	29396449
507	Ubiquitination	KRAHDCRKTAENAAK HHHHHHHHHHHHHHH	59.42	-
519	Ubiquitination	AAKALLMKLDGSCGG HHHHHHHHHCCCCCC	43.31	-
603	Ubiquitination	DPLSYDVKPRGDSQR CCCCCCCCCCCCCCC	26.54	32015554
633	Acetylation	KEEMAELKAQLYLLE HHHHHHHHHHHHHHH	26.12	20167786
641	Acetylation	AQLYLLEKEKKALEL HHHHHHHHHHHHHHH	74.85	20167786
681	Phosphorylation	QKEQRMRSLSSTSSG HHHHHHHHHHCCCCC	24.22	28985074
683	Phosphorylation	EQRMRSLSSTSSGSK HHHHHHHHCCCCCCC	32.58	23312004
684	Phosphorylation	QRMRSLSSTSSGSKD HHHHHHHCCCCCCCC	37.29	28655764
685	Phosphorylation	RMRSLSSTSSGSKDK HHHHHHCCCCCCCCC	24.44	30576142
686	Phosphorylation	MRSLSSTSSGSKDKP HHHHHCCCCCCCCCC	34.35	28655764
687	Phosphorylation	RSLSSTSSGSKDKPG HHHHCCCCCCCCCCC	47.37	28102081
689	Phosphorylation	LSSTSSGSKDKPGKE HHCCCCCCCCCCCHH	39.36	23312004
702	Phosphorylation	KECADAASPALSLAE HHHHHHHCHHHHHHH	16.66	21712546
706	Phosphorylation	DAASPALSLAELRTT HHHCHHHHHHHHHHH	27.86	30576142
712	Phosphorylation	LSLAELRTTCSENEL HHHHHHHHHCCHHHH	44.79	27251275
713	Phosphorylation	SLAELRTTCSENELA HHHHHHHHCCHHHHH	13.96	27251275
715	Phosphorylation	AELRTTCSENELAAE HHHHHHCCHHHHHHH	41.17	28857561
766	Ubiquitination	AEFVNDLKRANSNLV HHHHHHHHHHHHHHH	52.94	32015554
770	Phosphorylation	NDLKRANSNLVAAYE HHHHHHHHHHHHHHH	30.47	28857561
789	Acetylation	KHQNKLKKLESQMMA HHHHHHHHHHHHHHH	68.85	30590313
792	Phosphorylation	NKLKKLESQMMAMVE HHHHHHHHHHHHHHH	33.54	20068231
793	Ubiquitination	KLKKLESQMMAMVER HHHHHHHHHHHHHHH	19.12	32015554
803	Phosphorylation	AMVERHETQVRMLKQ HHHHHHHHHHHHHHH	26.66	20068231
820	Phosphorylation	ALLEEENSRPHTNET HHHHHHHCCCCCCCC	50.93	29255136
824	Phosphorylation	EENSRPHTNETSL-- HHHCCCCCCCCCC--	37.12	29255136
827	Phosphorylation	SRPHTNETSL----- CCCCCCCCCC-----	37.08	30266825
828	Phosphorylation	RPHTNETSL------ CCCCCCCCC------	26.38	30266825
871	Phosphorylation	------------------------------------------------- -------------------------------------------------		27251275
873	Phosphorylation	--------------------------------------------------- ---------------------------------------------------		27251275
892	Phosphorylation	---------------------------------------------------------------------- ----------------------------------------------------------------------		27251275
896	Phosphorylation	-------------------------------------------------------------------------- --------------------------------------------------------------------------		27251275
905	Phosphorylation	----------------------------------------------------------------------------------- -----------------------------------------------------------------------------------		27251275
956	Ubiquitination	-------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------		32015554
1018	Phosphorylation	---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
118	S	Phosphorylation	Kinase	ATR	Q13535	PSP
118	S	Phosphorylation	Kinase	DNAPK	P78527	PSP
120	S	Phosphorylation	Kinase	ATR	Q13535	PSP
120	S	Phosphorylation	Kinase	DNAPK	P78527	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of CRCM_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CRCM_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CLH1_HUMAN	CLTC	physical	17353931
STRN_HUMAN	STRN	physical	17353931
STK24_HUMAN	STK24	physical	17353931
STRN3_HUMAN	STRN3	physical	17353931
STRN4_HUMAN	STRN4	physical	17353931
SYEP_HUMAN	EPRS	physical	17353931
2AAA_HUMAN	PPP2R1A	physical	17353931
TERA_HUMAN	VCP	physical	17353931
KC1D_HUMAN	CSNK1D	physical	17353931
MYH10_HUMAN	MYH10	physical	17353931
K1C18_HUMAN	KRT18	physical	17353931
TPM3_HUMAN	TPM3	physical	17353931
KC1E_HUMAN	CSNK1E	physical	17353931
EIF3A_HUMAN	EIF3A	physical	17353931
PUR4_HUMAN	PFAS	physical	17353931
SYIC_HUMAN	IARS	physical	17353931
SYTC_HUMAN	TARS	physical	17353931
PFKAP_HUMAN	PFKP	physical	17353931
U520_HUMAN	SNRNP200	physical	17353931
C1TC_HUMAN	MTHFD1	physical	17353931
PDC10_HUMAN	PDCD10	physical	17353931
PSME3_HUMAN	PSME3	physical	17353931
SYMC_HUMAN	MARS	physical	17353931
CBR1_HUMAN	CBR1	physical	17353931
VDAC1_HUMAN	VDAC1	physical	17353931
STRP1_HUMAN	STRIP1	physical	17353931
HPRT_HUMAN	HPRT1	physical	17353931
PSMD2_HUMAN	PSMD2	physical	17353931
EZRI_HUMAN	EZR	physical	17353931
PUR6_HUMAN	PAICS	physical	17353931
VPS35_HUMAN	VPS35	physical	17353931
LKHA4_HUMAN	LTA4H	physical	17353931
A4_HUMAN	APP	physical	21832049

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CRCM_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294, AND MASSSPECTROMETRY.	19413330 [Abstract]