dbPTM

LKHA4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	LKHA4_HUMAN
UniProt AC	P09960
Protein Name	Leukotriene A-4 hydrolase
Gene Name	LTA4H
Organism	Homo sapiens (Human).
Sequence Length	611
Subcellular Localization	Cytoplasm.
Protein Description	Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity..
Protein Sequence	MPEIVDTCSLASPASVCRTKHLHLRCSVDFTRRTLTGTAALTVQSQEDNLRSLVLDTKDLTIEKVVINGQEVKYALGERQSYKGSPMEISLPIALSKNQEIVIEISFETSPKSSALQWLTPEQTSGKEHPYLFSQCQAIHCRAILPCQDTPSVKLTYTAEVSVPKELVALMSAIRDGETPDPEDPSRKIYKFIQKVPIPCYLIALVVGALESRQIGPRTLVWSEKEQVEKSAYEFSETESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGELQNSVKTFGETHPFTKLVVDLTDIDPDVAYSSVPYEKGFALLFYLEQLLGGPEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVDWNAWLYSPGLPPIKPNYDMTLTNACIALSQRWITAKEDDLNSFNATDLKDLSSHQLNEFLAQTLQRAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWEDAIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAVRTYQEHKASMHPVTAMLVGKDLKVD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
12	Phosphorylation	VDTCSLASPASVCRT CCCCCCCCCHHHHCC	26.71	25159151
15	Phosphorylation	CSLASPASVCRTKHL CCCCCCHHHHCCCCE	25.74	21815630
17	Glutathionylation	LASPASVCRTKHLHL CCCCHHHHCCCCEEE	4.18	22555962
20	Ubiquitination	PASVCRTKHLHLRCS CHHHHCCCCEEEEEE	25.47	21890473
20	Acetylation	PASVCRTKHLHLRCS CHHHHCCCCEEEEEE	25.47	26210075
20 (in isoform 1)	Ubiquitination	-	25.47	21890473
20 (in isoform 2)	Ubiquitination	-	25.47	21890473
20	Ubiquitination	PASVCRTKHLHLRCS CHHHHCCCCEEEEEE	25.47	22053931
27	Phosphorylation	KHLHLRCSVDFTRRT CCEEEEEEEECCCCC	20.49	27251275
31	Phosphorylation	LRCSVDFTRRTLTGT EEEEEECCCCCCCEE	18.12	25690035
34	Phosphorylation	SVDFTRRTLTGTAAL EEECCCCCCCEEEEE	25.94	-
42	O-linked_Glycosylation	LTGTAALTVQSQEDN CCEEEEEEEECCCHH	16.27	23301498
45	O-linked_Glycosylation	TAALTVQSQEDNLRS EEEEEEECCCHHHHH	31.07	23301498
49	Ubiquitination	TVQSQEDNLRSLVLD EEECCCHHHHHEEEE	36.28	21890473
49	Acetylation	TVQSQEDNLRSLVLD EEECCCHHHHHEEEE	36.28	19608861
49	Ubiquitination	TVQSQEDNLRSLVLD EEECCCHHHHHEEEE	36.28	19608861
58	Acetylation	RSLVLDTKDLTIEKV HHEEEECCCCEEEEE	50.86	23236377
58	Ubiquitination	RSLVLDTKDLTIEKV HHEEEECCCCEEEEE	50.86	-
58	2-Hydroxyisobutyrylation	RSLVLDTKDLTIEKV HHEEEECCCCEEEEE	50.86	-
58	Succinylation	RSLVLDTKDLTIEKV HHEEEECCCCEEEEE	50.86	23954790
64	Ubiquitination	TKDLTIEKVVINGQE CCCCEEEEEEECCEE	37.64	-
73	Acetylation	VINGQEVKYALGERQ EECCEEEEEEECCCC	24.85	19608861
73	Ubiquitination	VINGQEVKYALGERQ EECCEEEEEEECCCC	24.85	73
73 (in isoform 2)	Ubiquitination	-	24.85	21890473
73 (in isoform 1)	Ubiquitination	-	24.85	21890473
74	Phosphorylation	INGQEVKYALGERQS ECCEEEEEEECCCCC	16.54	26437602
81	Phosphorylation	YALGERQSYKGSPME EEECCCCCCCCCCCE	34.73	25159151
83	Ubiquitination	LGERQSYKGSPMEIS ECCCCCCCCCCCEEE	59.15	-
85	Phosphorylation	ERQSYKGSPMEISLP CCCCCCCCCCEEEEE	19.54	22210691
87	Sulfoxidation	QSYKGSPMEISLPIA CCCCCCCCEEEEEEE	8.38	30846556
90	Phosphorylation	KGSPMEISLPIALSK CCCCCEEEEEEEECC	18.13	29396449
96	Phosphorylation	ISLPIALSKNQEIVI EEEEEEECCCCEEEE	22.12	29396449
106	Phosphorylation	QEIVIEISFETSPKS CEEEEEEEEECCCCC	12.30	23663014
110	Phosphorylation	IEISFETSPKSSALQ EEEEEECCCCCCCCC	24.11	23663014
127	Ubiquitination	TPEQTSGKEHPYLFS CCCCCCCCCCCCHHH	53.25	-
150	Phosphorylation	AILPCQDTPSVKLTY EEEECCCCCCEEEEE	7.50	23403867
152	Phosphorylation	LPCQDTPSVKLTYTA EECCCCCCEEEEEEE	33.70	23403867
154	Ubiquitination	CQDTPSVKLTYTAEV CCCCCCEEEEEEEEE	39.18	-
156	Phosphorylation	DTPSVKLTYTAEVSV CCCCEEEEEEEEEEC	17.07	28152594
157	Phosphorylation	TPSVKLTYTAEVSVP CCCEEEEEEEEEECC	17.87	28152594
158	Phosphorylation	PSVKLTYTAEVSVPK CCEEEEEEEEEECCH	15.88	28152594
162	Phosphorylation	LTYTAEVSVPKELVA EEEEEEEECCHHHHH	24.98	23403867
171	Sulfoxidation	PKELVALMSAIRDGE CHHHHHHHHHHHCCC	1.51	30846556
172	Phosphorylation	KELVALMSAIRDGET HHHHHHHHHHHCCCC	22.76	20068231
186	Phosphorylation	TPDPEDPSRKIYKFI CCCCCCCCHHHHHHH	59.53	21712546
188	Ubiquitination	DPEDPSRKIYKFIQK CCCCCCHHHHHHHHC	55.51	-
191	Ubiquitination	DPSRKIYKFIQKVPI CCCHHHHHHHHCCCC	39.19	-
195	Acetylation	KIYKFIQKVPIPCYL HHHHHHHCCCCCHHH	45.20	21466224
212	Phosphorylation	LVVGALESRQIGPRT HHHHHHHHCCCCCCE	30.27	19690332
225	Ubiquitination	RTLVWSEKEQVEKSA CEEEECCHHHHHHHH	48.18	-
225	Acetylation	RTLVWSEKEQVEKSA CEEEECCHHHHHHHH	48.18	25953088
230	Ubiquitination	SEKEQVEKSAYEFSE CCHHHHHHHHHCCCH	41.34	21906983
230 (in isoform 2)	Ubiquitination	-	41.34	21890473
230 (in isoform 1)	Ubiquitination	-	41.34	21890473
231	Phosphorylation	EKEQVEKSAYEFSET CHHHHHHHHHCCCHH	23.68	28152594
233	Phosphorylation	EQVEKSAYEFSETES HHHHHHHHCCCHHHH	25.29	28152594
236	Phosphorylation	EKSAYEFSETESMLK HHHHHCCCHHHHHHH	31.50	-
238	Phosphorylation	SAYEFSETESMLKIA HHHCCCHHHHHHHHH	31.96	28348404
240	Phosphorylation	YEFSETESMLKIAED HCCCHHHHHHHHHHH	36.51	28348404
313	Acetylation	LVTNKTWDHFWLNEG EEECCCEEEEEECCC	32.13	19608861
313	Ubiquitination	LVTNKTWDHFWLNEG EEECCCEEEEEECCC	32.13	19608861
337	Acetylation	CGRLFGEKFRHFNAL HHHHHHHHCCCCCCC	48.34	19608861
337	Ubiquitination	CGRLFGEKFRHFNAL HHHHHHHHCCCCCCC	48.34	19608861
337	2-Hydroxyisobutyrylation	CGRLFGEKFRHFNAL HHHHHHHHCCCCCCC	48.34	-
337	Malonylation	CGRLFGEKFRHFNAL HHHHHHHHCCCCCCC	48.34	32601280
355	Ubiquitination	GELQNSVKTFGETHP HHHHHHHCCCCCCCC	37.65	-
355 (in isoform 2)	Ubiquitination	-	37.65	-
355	Acetylation	GELQNSVKTFGETHP HHHHHHHCCCCCCCC	37.65	26051181
365	Ubiquitination	GETHPFTKLVVDLTD CCCCCCCEEEEECCC	39.40	-
379	Phosphorylation	DIDPDVAYSSVPYEK CCCCCCCCCCCCHHH	11.00	25147952
390	Ubiquitination	PYEKGFALLFYLEQL CHHHHHHHHHHHHHH	2.95	21890473
390	Acetylation	PYEKGFALLFYLEQL CHHHHHHHHHHHHHH	2.95	19608861
390	Ubiquitination	PYEKGFALLFYLEQL CHHHHHHHHHHHHHH	2.95	19608861
394	Ubiquitination	GFALLFYLEQLLGGP HHHHHHHHHHHHCCH	2.37	21890473
414	Acetylation	FLKAYVEKFSYKSIT HHHHHHHHCCCCCCC	29.57	19608861
414	Ubiquitination	FLKAYVEKFSYKSIT HHHHHHHHCCCCCCC	29.57	21890473
414	2-Hydroxyisobutyrylation	FLKAYVEKFSYKSIT HHHHHHHHCCCCCCC	29.57	-
414 (in isoform 1)	Ubiquitination	-	29.57	21890473
414 (in isoform 2)	Ubiquitination	-	29.57	21890473
416	Phosphorylation	KAYVEKFSYKSITTD HHHHHHCCCCCCCCC	42.60	29496963
417	Phosphorylation	AYVEKFSYKSITTDD HHHHHCCCCCCCCCH	16.34	22817900
418	Acetylation	YVEKFSYKSITTDDW HHHHCCCCCCCCCHH	33.90	23236377
418	Ubiquitination	YVEKFSYKSITTDDW HHHHCCCCCCCCCHH	33.90	21890473
418 (in isoform 2)	Ubiquitination	-	33.90	21890473
418	2-Hydroxyisobutyrylation	YVEKFSYKSITTDDW HHHHCCCCCCCCCHH	33.90	-
418 (in isoform 1)	Ubiquitination	-	33.90	21890473
430	Phosphorylation	DDWKDFLYSYFKDKV CHHHHHHHHHHCCHH	11.00	-
434	Acetylation	DFLYSYFKDKVDVLN HHHHHHHCCHHCCHH	48.70	23954790
434	Ubiquitination	DFLYSYFKDKVDVLN HHHHHHHCCHHCCHH	48.70	-
434	Succinylation	DFLYSYFKDKVDVLN HHHHHHHCCHHCCHH	48.70	23954790
456	Ubiquitination	LYSPGLPPIKPNYDM HCCCCCCCCCCCCCC	52.88	21890473
480	Acetylation	SQRWITAKEDDLNSF HHHCHHCCHHHHHCC	53.69	23954790
480	Ubiquitination	SQRWITAKEDDLNSF HHHCHHCCHHHHHCC	53.69	21890473
480 (in isoform 2)	Ubiquitination	-	53.69	21890473
480 (in isoform 1)	Ubiquitination	-	53.69	21890473
486	Phosphorylation	AKEDDLNSFNATDLK CCHHHHHCCCCCCHH	27.46	29514088
490	Phosphorylation	DLNSFNATDLKDLSS HHHCCCCCCHHHCCH	42.86	22985185
493	Ubiquitination	SFNATDLKDLSSHQL CCCCCCHHHCCHHHH	60.47	-
496	Phosphorylation	ATDLKDLSSHQLNEF CCCHHHCCHHHHHHH	35.59	20873877
497	Phosphorylation	TDLKDLSSHQLNEFL CCHHHCCHHHHHHHH	23.66	20873877
507	Phosphorylation	LNEFLAQTLQRAPLP HHHHHHHHHHHCCCC	21.17	20873877
519	Ubiquitination	PLPLGHIKRMQEVYN CCCHHHHHHHHHHHC	35.82	-
519	Acetylation	PLPLGHIKRMQEVYN CCCHHHHHHHHHHHC	35.82	25953088
521	Sulfoxidation	PLGHIKRMQEVYNFN CHHHHHHHHHHHCCC	3.19	30846556
549	Acetylation	LCIQSKWEDAIPLAL HHHHHCHHHHHHHHH	41.10	19608861
549	Ubiquitination	LCIQSKWEDAIPLAL HHHHHCHHHHHHHHH	41.10	19608861
557	Ubiquitination	DAIPLALKMATEQGR HHHHHHHHHHHHCCC	22.38	-
566	Ubiquitination	ATEQGRMKFTRPLFK HHHCCCCCCCHHHHH	42.07	-
573	Acetylation	KFTRPLFKDLAAFDK CCCHHHHHHHHHHCC	60.56	19608861
573	Ubiquitination	KFTRPLFKDLAAFDK CCCHHHHHHHHHHCC	60.56	21890473
573	Succinylation	KFTRPLFKDLAAFDK CCCHHHHHHHHHHCC	60.56	23954790
573 (in isoform 1)	Ubiquitination	-	60.56	21890473
580	Ubiquitination	KDLAAFDKSHDQAVR HHHHHHCCCHHHHHH	43.14	-
580	2-Hydroxyisobutyrylation	KDLAAFDKSHDQAVR HHHHHHCCCHHHHHH	43.14	-
580	Acetylation	KDLAAFDKSHDQAVR HHHHHHCCCHHHHHH	43.14	25953088
589	Phosphorylation	HDQAVRTYQEHKASM HHHHHHHHHHHHHCC	11.01	18767875
593	Ubiquitination	VRTYQEHKASMHPVT HHHHHHHHHCCCCCH	41.21	21890473
593 (in isoform 1)	Ubiquitination	-	41.21	21890473
595	Phosphorylation	TYQEHKASMHPVTAM HHHHHHHCCCCCHHH	23.56	18767875
600	Phosphorylation	KASMHPVTAMLVGKD HHCCCCCHHHHHCCC	15.71	18767875
606	Ubiquitination	VTAMLVGKDLKVD-- CHHHHHCCCCCCC--	53.02	-
606	Acetylation	VTAMLVGKDLKVD-- CHHHHHCCCCCCC--	53.02	25953088

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of LKHA4_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
416	S	Phosphorylation		9395533
Phosphorylation at Ser-416 inhibits leukotriene-A4 hydrolase activity.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of LKHA4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TMM62_HUMAN	TMEM62	physical	16169070
SIR1_HUMAN	SIRT1	physical	22939629
ZN593_HUMAN	ZNF593	physical	22939629
PNCB_HUMAN	NAPRT	physical	26344197
TAGL2_HUMAN	TAGLN2	physical	26344197

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
DB01197	Captopril

Regulatory Network of LKHA4_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-73; LYS-337; LYS-414 ANDLYS-573, AND MASS SPECTROMETRY.	19608861 [Abstract]

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