VPS35_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: VPS35_HUMAN
• UniProt AC: Q96QK1
• Protein Name: Vacuolar protein sorting-associated protein 35
• Gene Name: VPS35
• Organism: Homo sapiens (Human).
• Sequence Length: 796
• Subcellular Localization: Cytoplasm. Membrane; Peripheral membrane protein. Endosome . Early endosome . Late endosome . Localizes to tubular profiles adjacent to endosomes.
• Protein Description: Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). [PubMed: 15078903]
• Protein Sequence: MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MPTTQQSPQD -----CCCCCCCCHH	35.62	24732914
4	Phosphorylation	----MPTTQQSPQDE ----CCCCCCCCHHH	20.81	21955146
7	Phosphorylation	-MPTTQQSPQDEQEK -CCCCCCCCHHHHHH	18.08	23401153
14	Ubiquitination	SPQDEQEKLLDEAIQ CCHHHHHHHHHHHHH	54.78	24816145
24	Ubiquitination	DEAIQAVKVQSFQMK HHHHHHHHHCHHHHH	37.26	21906983
31	Acetylation	KVQSFQMKRCLDKNK HHCHHHHHHHCCHHH	29.22	25953088
38	2-Hydroxyisobutyrylation	KRCLDKNKLMDALKH HHHCCHHHHHHHHHH	51.43	-
38	Malonylation	KRCLDKNKLMDALKH HHHCCHHHHHHHHHH	51.43	26320211
38	Acetylation	KRCLDKNKLMDALKH HHHCCHHHHHHHHHH	51.43	25953088
44	Acetylation	NKLMDALKHASNMLG HHHHHHHHHHHHHHH	38.55	25953088
44	Ubiquitination	NKLMDALKHASNMLG HHHHHHHHHHHHHHH	38.55	32015554
47	Phosphorylation	MDALKHASNMLGELR HHHHHHHHHHHHHHH	23.61	20860994
59	Phosphorylation	ELRTSMLSPKSYYEL HHHHHCCCCHHHHHH	22.95	-
64	Ubiquitination	MLSPKSYYELYMAIS CCCCHHHHHHHHHHH	13.42	24816145
67	Phosphorylation	PKSYYELYMAISDEL CHHHHHHHHHHHCHH	3.44	-
90	Ubiquitination	DEFAKGRKVADLYEL HHHHCCCCHHHHHHH	50.90	33845483
102	Ubiquitination	YELVQYAGNIIPRLY HHHHHHHCCHHHHHH	22.42	24816145
109	Phosphorylation	GNIIPRLYLLITVGV CCHHHHHHHHHHHEE	10.76	20068231
125	Phosphorylation	YVKSFPQSRKDILKD HHHCCCHHHHHHHHH	41.35	20068231
131	Ubiquitination	QSRKDILKDLVEMCR HHHHHHHHHHHHHHH	49.72	24816145
137	Glutathionylation	LKDLVEMCRGVQHPL HHHHHHHHHHCCCHH	1.85	22555962
145	Methylation	RGVQHPLRGLFLRNY HHCCCHHHHHHHHHH	44.19	115919853
177	Phosphorylation	EETTGDISDSMDFVL CCCCCCCCHHCCEEE	28.51	21601212
329	Ubiquitination	IKLFDIFSQQVATVI HHHHHHHHHHHHHHH	21.75	21890473
350	Ubiquitination	PSEDVVSLQVSLINL CCCCCHHHHHHHHHH	3.83	21890473
353	Phosphorylation	DVVSLQVSLINLAMK CCHHHHHHHHHHHHH	15.57	-
367	Ubiquitination	KCYPDRVDYVDKVLE HHCCCCCHHHHHHHH	38.34	21890473
371	Ubiquitination	DRVDYVDKVLETTVE CCCHHHHHHHHHHHH	38.38	29967540
382	Ubiquitination	TTVEIFNKLNLEHIA HHHHHHHHCCHHHHH	28.18	29967540
388	Ubiquitination	NKLNLEHIATSSAVS HHCCHHHHHCCHHHH	3.06	21890473
390	Phosphorylation	LNLEHIATSSAVSKE CCHHHHHCCHHHHHH	23.70	20068231
391	Phosphorylation	NLEHIATSSAVSKEL CHHHHHCCHHHHHHH	13.79	20068231
392	Phosphorylation	LEHIATSSAVSKELT HHHHHCCHHHHHHHH	28.47	20068231
395	Phosphorylation	IATSSAVSKELTRLL HHCCHHHHHHHHHHH	21.82	20068231
396	Ubiquitination	ATSSAVSKELTRLLK HCCHHHHHHHHHHHC	50.58	22817900
403	Ubiquitination	KELTRLLKIPVDTYN HHHHHHHCCCHHHHH	49.94	29967540
408	Phosphorylation	LLKIPVDTYNNILTV HHCCCHHHHHCHHHH	28.51	29759185
414	Phosphorylation	DTYNNILTVLKLKHF HHHHCHHHHHHHCCC	21.72	29759185
417	Ubiquitination	NNILTVLKLKHFHPL HCHHHHHHHCCCCHH	51.67	21890473
419	Acetylation	ILTVLKLKHFHPLFE HHHHHHHCCCCHHHH	42.51	27452117
419	Ubiquitination	ILTVLKLKHFHPLFE HHHHHHHCCCCHHHH	42.51	29967540
432	Phosphorylation	FEYFDYESRKSMSCY HHHCCCCCCCCCHHE	38.49	-
435	Phosphorylation	FDYESRKSMSCYVLS CCCCCCCCCHHEHHH	18.15	-
437	Phosphorylation	YESRKSMSCYVLSNV CCCCCCCHHEHHHHH	14.91	-
448	Ubiquitination	LSNVLDYNTEIVSQD HHHHCCCCCCCCCHH	30.79	22053931
486	Ubiquitination	PDPEDFADEQSLVGR CCHHHCCCHHHHHHH	55.23	22053931
507	Phosphorylation	SEDPDQQYLILNTAR CCCCCCCEEEEECCH	7.06	28152594
512	Phosphorylation	QQYLILNTARKHFGA CCEEEEECCHHHCCC	25.13	-
515	Ubiquitination	LILNTARKHFGAGGN EEEECCHHHCCCCCC	40.60	21890473
547	Phosphorylation	AFRYKENSKVDDKWE HHHHHHCCCCCHHHH	34.84	28270605
552	Acetylation	ENSKVDDKWEKKCQK HCCCCCHHHHHHHHH	53.43	25953088
555	Acetylation	KVDDKWEKKCQKIFS CCCHHHHHHHHHHHH	58.70	23749302
572	Ubiquitination	HQTISALIKAELAEL HHHHHHHHHHHHHHC	3.88	24816145
573	Ubiquitination	QTISALIKAELAELP HHHHHHHHHHHHHCC	36.34	33845483
592	Ubiquitination	LQGALAAGEIGFENH HHHHHHHCCCCCCCC	22.80	27667366
595	Ubiquitination	ALAAGEIGFENHETV HHHHCCCCCCCCHHH	21.36	24816145
610	Ubiquitination	AYEFMSQAFSLYEDE HHHHHHHHHHHHHHH	6.68	24816145
627	Ubiquitination	DSKAQLAAITLIIGT CCHHHHHHHHHHHHH	12.14	27667366
629	Phosphorylation	KAQLAAITLIIGTFE HHHHHHHHHHHHHHH	13.74	20068231
630	Ubiquitination	AQLAAITLIIGTFER HHHHHHHHHHHHHHH	1.88	27667366
633	Ubiquitination	AAITLIIGTFERMKC HHHHHHHHHHHHHHC	19.84	24816145
639	Ubiquitination	IGTFERMKCFSEENH HHHHHHHHCCCCCCC	38.05	24816145
658	Phosphorylation	TQCALAASKLLKKPD HHHHHHHHHHCCCCC	20.44	28857561
659	Ubiquitination	QCALAASKLLKKPDQ HHHHHHHHHCCCCCC	53.78	33845483
659	Acetylation	QCALAASKLLKKPDQ HHHHHHHHHCCCCCC	53.78	25953088
662	Acetylation	LAASKLLKKPDQGRA HHHHHHCCCCCCCCC	73.10	25953088
662	2-Hydroxyisobutyrylation	LAASKLLKKPDQGRA HHHHHHCCCCCCCCC	73.10	-
662	Ubiquitination	LAASKLLKKPDQGRA HHHHHHCCCCCCCCC	73.10	24816145
665	Ubiquitination	SKLLKKPDQGRAVST HHHCCCCCCCCCCHH	72.88	27667366
671	Phosphorylation	PDQGRAVSTCAHLFW CCCCCCCHHHHHHHH	19.45	28857561
683	Phosphorylation	LFWSGRNTDKNGEEL HHHCCCCCCCCCCCC	47.17	-
694	2-Hydroxyisobutyrylation	GEELHGGKRVMECLK CCCCCCCHHHHHHHH	46.54	-
694	Ubiquitination	GEELHGGKRVMECLK CCCCCCCHHHHHHHH	46.54	27667366
694	Acetylation	GEELHGGKRVMECLK CCCCCCCHHHHHHHH	46.54	7619357
701	Acetylation	KRVMECLKKALKIAN HHHHHHHHHHHHHHH	47.89	25953088
714	Phosphorylation	ANQCMDPSLQVQLFI HHHHCCHHHHHHHHH	27.46	20068231
759	Phosphorylation	EDLPNLESSEETEQI HHCCCCCCHHHHHHH	46.33	25627689
760	Phosphorylation	DLPNLESSEETEQIN HCCCCCCHHHHHHHH	30.03	25627689
768	Acetylation	EETEQINKHFHNTLE HHHHHHHHHHHHHHH	49.64	21466224
768	Ubiquitination	EETEQINKHFHNTLE HHHHHHHHHHHHHHH	49.64	29967540
783	Phosphorylation	HLRLRRESPESEGPI HHHHHHCCCCCCCCC	31.96	19664994
786	Phosphorylation	LRRESPESEGPIYEG HHHCCCCCCCCCCCC	51.76	30266825
791	Phosphorylation	PESEGPIYEGLIL-- CCCCCCCCCCCCC--	13.69	28796482

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of VPS35_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of VPS35_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of VPS35_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
VPS35_YEAST	VPS35	genetic	17916227
WAC2C_HUMAN	FAM21C	physical	20923837
TBCD5_HUMAN	TBC1D5	physical	22354992
A4_HUMAN	APP	physical	21832049
VPS36_HUMAN	VPS36	physical	22939629
WDR5_HUMAN	WDR5	physical	22939629
VPS35_HUMAN	VPS35	physical	23452853
AN32E_HUMAN	ANP32E	physical	22863883
COR1C_HUMAN	CORO1C	physical	22863883
NIBL1_HUMAN	FAM129B	physical	22863883
H33_HUMAN	H3F3A	physical	22863883
NUDC_HUMAN	NUDC	physical	22863883
SAP_HUMAN	PSAP	physical	22863883
GLYM_HUMAN	SHMT2	physical	22863883
SNX2_HUMAN	SNX2	physical	22863883
SNX6_HUMAN	SNX6	physical	22863883
UBP48_HUMAN	USP48	physical	22863883
SNX3_HUMAN	SNX3	physical	24344282
NRAM2_HUMAN	SLC11A2	physical	24344282
VP26A_HUMAN	VPS26A	physical	24344282
VPS29_HUMAN	VPS29	physical	24344282
VP13A_HUMAN	VPS13A	physical	26344197
VPS29_HUMAN	VPS29	physical	26344197
AIMP2_HUMAN	AIMP2	physical	28383562
LAMP2_HUMAN	LAMP2	physical	28383562

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 614203: Parkinson disease 17 (PARK17)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.